Profiling Lgals9 splice variant expression at the fetal-maternal interface: implications in normal and pathological human pregnancy

Autores
Heusschen, Roy; Freitag, Nancy; Tirado González, Irene; Barrientos, Gabriela Laura; Moschansky, Petra; Muñoz Fernández, Raquel; Leno Durán, Ester; Klapp, Burghard F.; Thijssen, Victor L.J.L.; Blois, Sandra M.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Disruption of fetal-maternal tolerance mechanisms can contribute to pregnancy complications, including spontaneous abortion. Galectin-9 (LGALS9), a tandem repeat lectin associated with immune modulation, is expressed in the endometrium during the mid and late secretory phases and in decidua during human early pregnancy. However, the role of LGALS9 during pregnancy remains poorly understood. We used real-time PCR and immunohistochemical staining to analyze the expression of Lgals9/LGALS9 during mouse gestation as well as in human tissues obtained from normal pregnancy and spontaneous abortions. In mice, three Lgals9 splice variants were detected, the expression of which was differentially regulated during gestation. Furthermore, decidual Lgals9 expression was deregulated in a mouse model of spontaneous abortion, whereas placental levels did not change. We further found that the LGALS9 D5 isoform suppresses interferon gamma production by decidual natural killer cells. In human patients, six Lgals9 splice variants were detected, and a decrease in Lgals9 D5/10 was associated with spontaneous abortion. Altogether, these results show a differential regulation of Lgals9 isoform expression during normal and pathological pregnancies and designate Lgals9 as a potential marker for adverse pregnancy outcomes.
Fil: Heusschen, Roy. VU University Medical Center; Países Bajos
Fil: Freitag, Nancy. Medicine University of Berlin; Alemania
Fil: Tirado González, Irene. Medicine University of Berlin; Alemania
Fil: Barrientos, Gabriela Laura. Medicine University of Berlin; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Moschansky, Petra. Medicine University of Berlin; Alemania
Fil: Muñoz Fernández, Raquel. Consejo Superior de Investigaciones Cientificas; España
Fil: Leno Durán, Ester. Universidad de Granada; España
Fil: Klapp, Burghard F.. Medicine University of Berlin; Alemania
Fil: Thijssen, Victor L.J.L.. VU University Medical Center; Países Bajos
Fil: Blois, Sandra M.. Medicine University of Berlin; Alemania
Materia
Ifng
Lgals9
Normal Pregnancy
Splice Variants
Spontaneous Abortion
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/16511
Acceder
id CONICETDig_83a2e351dccef14208e41a16946788fb
oai_identifier_str oai:ri.conicet.gov.ar:11336/16511
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Profiling Lgals9 splice variant expression at the fetal-maternal interface: implications in normal and pathological human pregnancyHeusschen, RoyFreitag, NancyTirado González, IreneBarrientos, Gabriela LauraMoschansky, PetraMuñoz Fernández, RaquelLeno Durán, EsterKlapp, Burghard F.Thijssen, Victor L.J.L.Blois, Sandra M.IfngLgals9Normal PregnancySplice VariantsSpontaneous Abortionhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Disruption of fetal-maternal tolerance mechanisms can contribute to pregnancy complications, including spontaneous abortion. Galectin-9 (LGALS9), a tandem repeat lectin associated with immune modulation, is expressed in the endometrium during the mid and late secretory phases and in decidua during human early pregnancy. However, the role of LGALS9 during pregnancy remains poorly understood. We used real-time PCR and immunohistochemical staining to analyze the expression of Lgals9/LGALS9 during mouse gestation as well as in human tissues obtained from normal pregnancy and spontaneous abortions. In mice, three Lgals9 splice variants were detected, the expression of which was differentially regulated during gestation. Furthermore, decidual Lgals9 expression was deregulated in a mouse model of spontaneous abortion, whereas placental levels did not change. We further found that the LGALS9 D5 isoform suppresses interferon gamma production by decidual natural killer cells. In human patients, six Lgals9 splice variants were detected, and a decrease in Lgals9 D5/10 was associated with spontaneous abortion. Altogether, these results show a differential regulation of Lgals9 isoform expression during normal and pathological pregnancies and designate Lgals9 as a potential marker for adverse pregnancy outcomes.Fil: Heusschen, Roy. VU University Medical Center; Países BajosFil: Freitag, Nancy. Medicine University of Berlin; AlemaniaFil: Tirado González, Irene. Medicine University of Berlin; AlemaniaFil: Barrientos, Gabriela Laura. Medicine University of Berlin; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Moschansky, Petra. Medicine University of Berlin; AlemaniaFil: Muñoz Fernández, Raquel. Consejo Superior de Investigaciones Cientificas; EspañaFil: Leno Durán, Ester. Universidad de Granada; EspañaFil: Klapp, Burghard F.. Medicine University of Berlin; AlemaniaFil: Thijssen, Victor L.J.L.. VU University Medical Center; Países BajosFil: Blois, Sandra M.. Medicine University of Berlin; AlemaniaOxford University Press2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16511Heusschen, Roy; Freitag, Nancy; Tirado González, Irene; Barrientos, Gabriela Laura; Moschansky, Petra; et al.; Profiling Lgals9 splice variant expression at the fetal-maternal interface: implications in normal and pathological human pregnancy; Oxford University Press; Biology Of Reproduction; 88; 1; 2013; 1-10; 220006-33631529-7268enginfo:eu-repo/semantics/altIdentifier/doi/10.1095/biolreprod.112.105460info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/biolreprod/article/2514168/Profilinginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:36:30Zoai:ri.conicet.gov.ar:11336/16511instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:36:30.24CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Profiling Lgals9 splice variant expression at the fetal-maternal interface: implications in normal and pathological human pregnancy
title Profiling Lgals9 splice variant expression at the fetal-maternal interface: implications in normal and pathological human pregnancy
spellingShingle Profiling Lgals9 splice variant expression at the fetal-maternal interface: implications in normal and pathological human pregnancy
Heusschen, Roy
Ifng
Lgals9
Normal Pregnancy
Splice Variants
Spontaneous Abortion
title_short Profiling Lgals9 splice variant expression at the fetal-maternal interface: implications in normal and pathological human pregnancy
title_full Profiling Lgals9 splice variant expression at the fetal-maternal interface: implications in normal and pathological human pregnancy
title_fullStr Profiling Lgals9 splice variant expression at the fetal-maternal interface: implications in normal and pathological human pregnancy
title_full_unstemmed Profiling Lgals9 splice variant expression at the fetal-maternal interface: implications in normal and pathological human pregnancy
title_sort Profiling Lgals9 splice variant expression at the fetal-maternal interface: implications in normal and pathological human pregnancy
dc.creator.none.fl_str_mv Heusschen, Roy
Freitag, Nancy
Tirado González, Irene
Barrientos, Gabriela Laura
Moschansky, Petra
Muñoz Fernández, Raquel
Leno Durán, Ester
Klapp, Burghard F.
Thijssen, Victor L.J.L.
Blois, Sandra M.
author Heusschen, Roy
author_facet Heusschen, Roy
Freitag, Nancy
Tirado González, Irene
Barrientos, Gabriela Laura
Moschansky, Petra
Muñoz Fernández, Raquel
Leno Durán, Ester
Klapp, Burghard F.
Thijssen, Victor L.J.L.
Blois, Sandra M.
author_role author
author2 Freitag, Nancy
Tirado González, Irene
Barrientos, Gabriela Laura
Moschansky, Petra
Muñoz Fernández, Raquel
Leno Durán, Ester
Klapp, Burghard F.
Thijssen, Victor L.J.L.
Blois, Sandra M.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ifng
Lgals9
Normal Pregnancy
Splice Variants
Spontaneous Abortion
topic Ifng
Lgals9
Normal Pregnancy
Splice Variants
Spontaneous Abortion
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Disruption of fetal-maternal tolerance mechanisms can contribute to pregnancy complications, including spontaneous abortion. Galectin-9 (LGALS9), a tandem repeat lectin associated with immune modulation, is expressed in the endometrium during the mid and late secretory phases and in decidua during human early pregnancy. However, the role of LGALS9 during pregnancy remains poorly understood. We used real-time PCR and immunohistochemical staining to analyze the expression of Lgals9/LGALS9 during mouse gestation as well as in human tissues obtained from normal pregnancy and spontaneous abortions. In mice, three Lgals9 splice variants were detected, the expression of which was differentially regulated during gestation. Furthermore, decidual Lgals9 expression was deregulated in a mouse model of spontaneous abortion, whereas placental levels did not change. We further found that the LGALS9 D5 isoform suppresses interferon gamma production by decidual natural killer cells. In human patients, six Lgals9 splice variants were detected, and a decrease in Lgals9 D5/10 was associated with spontaneous abortion. Altogether, these results show a differential regulation of Lgals9 isoform expression during normal and pathological pregnancies and designate Lgals9 as a potential marker for adverse pregnancy outcomes.
Fil: Heusschen, Roy. VU University Medical Center; Países Bajos
Fil: Freitag, Nancy. Medicine University of Berlin; Alemania
Fil: Tirado González, Irene. Medicine University of Berlin; Alemania
Fil: Barrientos, Gabriela Laura. Medicine University of Berlin; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Moschansky, Petra. Medicine University of Berlin; Alemania
Fil: Muñoz Fernández, Raquel. Consejo Superior de Investigaciones Cientificas; España
Fil: Leno Durán, Ester. Universidad de Granada; España
Fil: Klapp, Burghard F.. Medicine University of Berlin; Alemania
Fil: Thijssen, Victor L.J.L.. VU University Medical Center; Países Bajos
Fil: Blois, Sandra M.. Medicine University of Berlin; Alemania
description Disruption of fetal-maternal tolerance mechanisms can contribute to pregnancy complications, including spontaneous abortion. Galectin-9 (LGALS9), a tandem repeat lectin associated with immune modulation, is expressed in the endometrium during the mid and late secretory phases and in decidua during human early pregnancy. However, the role of LGALS9 during pregnancy remains poorly understood. We used real-time PCR and immunohistochemical staining to analyze the expression of Lgals9/LGALS9 during mouse gestation as well as in human tissues obtained from normal pregnancy and spontaneous abortions. In mice, three Lgals9 splice variants were detected, the expression of which was differentially regulated during gestation. Furthermore, decidual Lgals9 expression was deregulated in a mouse model of spontaneous abortion, whereas placental levels did not change. We further found that the LGALS9 D5 isoform suppresses interferon gamma production by decidual natural killer cells. In human patients, six Lgals9 splice variants were detected, and a decrease in Lgals9 D5/10 was associated with spontaneous abortion. Altogether, these results show a differential regulation of Lgals9 isoform expression during normal and pathological pregnancies and designate Lgals9 as a potential marker for adverse pregnancy outcomes.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/16511
Heusschen, Roy; Freitag, Nancy; Tirado González, Irene; Barrientos, Gabriela Laura; Moschansky, Petra; et al.; Profiling Lgals9 splice variant expression at the fetal-maternal interface: implications in normal and pathological human pregnancy; Oxford University Press; Biology Of Reproduction; 88; 1; 2013; 1-10; 22
0006-3363
1529-7268
url http://hdl.handle.net/11336/16511
identifier_str_mv Heusschen, Roy; Freitag, Nancy; Tirado González, Irene; Barrientos, Gabriela Laura; Moschansky, Petra; et al.; Profiling Lgals9 splice variant expression at the fetal-maternal interface: implications in normal and pathological human pregnancy; Oxford University Press; Biology Of Reproduction; 88; 1; 2013; 1-10; 22
0006-3363
1529-7268
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1095/biolreprod.112.105460
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/biolreprod/article/2514168/Profiling
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083488013352960
score 13.22299

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