Human sperm chemotaxis depends on critical levels of reactive oxygen species
- Autores
- Sánchez, Raúl Oscar; Sepúlveda, Carolina; Risopatrón, Jennie; Villegas, Juana; Giojalas, Laura Cecilia
- Año de publicación
- 2010
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Objective: To verify whether chemotaxis is in part an oxidative process mediated by reactive oxygen species (ROS). Design: In this prospective study, after removal of seminal plasma, the sperm suspension received no treatment (control), ROS formation by stimulation with phorbol 12-myristate 13-acetate (PMA), antioxidant treatment (with catalase), or PMA stimulus in the presence of catalase. At time zero and after 3 hours of incubation, the percentage of capacitated and oriented spermatozoa and the ROS levels were determined. Setting: Andrology laboratory in a medical research institution. Patient(s): Normal semen was obtained from eight men. Intervention(s): The semen samples were evaluated to determine the effect of ROS production by stimulation with PMA and/or antioxidant treatment (with catalase) on the percentage of capacitated and oriented spermatozoa. Main Outcome Measure(s): The sperm capacitation, chemotaxis and reactive oxygen species were assessed before and after PMA and/or antioxidant treatment. Result(s): Prolonged exposure to high quantities of ROS decrease the sperm chemotactic response, probably because of oxidative damage of the cell. However, this effect may be reduced by the addition of antioxidants like catalase. Conclusion(s): Similar to capacitation, chemotaxis seems to depend on the production of ROS, but in the latter process there may be a critical level of ROS necessary for chemotaxis to occur.
Fil: Sánchez, Raúl Oscar. Universidad de La Frontera; Chile
Fil: Sepúlveda, Carolina. Universidad de La Frontera; Chile
Fil: Risopatrón, Jennie. Universidad de La Frontera; Chile
Fil: Villegas, Juana. Universidad de La Frontera; Chile
Fil: Giojalas, Laura Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; Argentina - Materia
-
Antioxidant
Chemotaxis
Human Spermatozoa
Ros
Sperm Capacition - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/57744
Ver los metadatos del registro completo
id |
CONICETDig_13eb21e2931355bae163ca3c0ac3bf9f |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/57744 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Human sperm chemotaxis depends on critical levels of reactive oxygen speciesSánchez, Raúl OscarSepúlveda, CarolinaRisopatrón, JennieVillegas, JuanaGiojalas, Laura CeciliaAntioxidantChemotaxisHuman SpermatozoaRosSperm Capacitionhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Objective: To verify whether chemotaxis is in part an oxidative process mediated by reactive oxygen species (ROS). Design: In this prospective study, after removal of seminal plasma, the sperm suspension received no treatment (control), ROS formation by stimulation with phorbol 12-myristate 13-acetate (PMA), antioxidant treatment (with catalase), or PMA stimulus in the presence of catalase. At time zero and after 3 hours of incubation, the percentage of capacitated and oriented spermatozoa and the ROS levels were determined. Setting: Andrology laboratory in a medical research institution. Patient(s): Normal semen was obtained from eight men. Intervention(s): The semen samples were evaluated to determine the effect of ROS production by stimulation with PMA and/or antioxidant treatment (with catalase) on the percentage of capacitated and oriented spermatozoa. Main Outcome Measure(s): The sperm capacitation, chemotaxis and reactive oxygen species were assessed before and after PMA and/or antioxidant treatment. Result(s): Prolonged exposure to high quantities of ROS decrease the sperm chemotactic response, probably because of oxidative damage of the cell. However, this effect may be reduced by the addition of antioxidants like catalase. Conclusion(s): Similar to capacitation, chemotaxis seems to depend on the production of ROS, but in the latter process there may be a critical level of ROS necessary for chemotaxis to occur.Fil: Sánchez, Raúl Oscar. Universidad de La Frontera; ChileFil: Sepúlveda, Carolina. Universidad de La Frontera; ChileFil: Risopatrón, Jennie. Universidad de La Frontera; ChileFil: Villegas, Juana. Universidad de La Frontera; ChileFil: Giojalas, Laura Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; ArgentinaElsevier Science Inc2010-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/57744Sánchez, Raúl Oscar; Sepúlveda, Carolina; Risopatrón, Jennie; Villegas, Juana; Giojalas, Laura Cecilia; Human sperm chemotaxis depends on critical levels of reactive oxygen species; Elsevier Science Inc; Fertility and Sterility; 93; 1; 1-1-2010; 150-1530015-0282CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0015028208039472info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fertnstert.2008.09.049info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:31Zoai:ri.conicet.gov.ar:11336/57744instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:32.027CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Human sperm chemotaxis depends on critical levels of reactive oxygen species |
title |
Human sperm chemotaxis depends on critical levels of reactive oxygen species |
spellingShingle |
Human sperm chemotaxis depends on critical levels of reactive oxygen species Sánchez, Raúl Oscar Antioxidant Chemotaxis Human Spermatozoa Ros Sperm Capacition |
title_short |
Human sperm chemotaxis depends on critical levels of reactive oxygen species |
title_full |
Human sperm chemotaxis depends on critical levels of reactive oxygen species |
title_fullStr |
Human sperm chemotaxis depends on critical levels of reactive oxygen species |
title_full_unstemmed |
Human sperm chemotaxis depends on critical levels of reactive oxygen species |
title_sort |
Human sperm chemotaxis depends on critical levels of reactive oxygen species |
dc.creator.none.fl_str_mv |
Sánchez, Raúl Oscar Sepúlveda, Carolina Risopatrón, Jennie Villegas, Juana Giojalas, Laura Cecilia |
author |
Sánchez, Raúl Oscar |
author_facet |
Sánchez, Raúl Oscar Sepúlveda, Carolina Risopatrón, Jennie Villegas, Juana Giojalas, Laura Cecilia |
author_role |
author |
author2 |
Sepúlveda, Carolina Risopatrón, Jennie Villegas, Juana Giojalas, Laura Cecilia |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Antioxidant Chemotaxis Human Spermatozoa Ros Sperm Capacition |
topic |
Antioxidant Chemotaxis Human Spermatozoa Ros Sperm Capacition |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Objective: To verify whether chemotaxis is in part an oxidative process mediated by reactive oxygen species (ROS). Design: In this prospective study, after removal of seminal plasma, the sperm suspension received no treatment (control), ROS formation by stimulation with phorbol 12-myristate 13-acetate (PMA), antioxidant treatment (with catalase), or PMA stimulus in the presence of catalase. At time zero and after 3 hours of incubation, the percentage of capacitated and oriented spermatozoa and the ROS levels were determined. Setting: Andrology laboratory in a medical research institution. Patient(s): Normal semen was obtained from eight men. Intervention(s): The semen samples were evaluated to determine the effect of ROS production by stimulation with PMA and/or antioxidant treatment (with catalase) on the percentage of capacitated and oriented spermatozoa. Main Outcome Measure(s): The sperm capacitation, chemotaxis and reactive oxygen species were assessed before and after PMA and/or antioxidant treatment. Result(s): Prolonged exposure to high quantities of ROS decrease the sperm chemotactic response, probably because of oxidative damage of the cell. However, this effect may be reduced by the addition of antioxidants like catalase. Conclusion(s): Similar to capacitation, chemotaxis seems to depend on the production of ROS, but in the latter process there may be a critical level of ROS necessary for chemotaxis to occur. Fil: Sánchez, Raúl Oscar. Universidad de La Frontera; Chile Fil: Sepúlveda, Carolina. Universidad de La Frontera; Chile Fil: Risopatrón, Jennie. Universidad de La Frontera; Chile Fil: Villegas, Juana. Universidad de La Frontera; Chile Fil: Giojalas, Laura Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; Argentina |
description |
Objective: To verify whether chemotaxis is in part an oxidative process mediated by reactive oxygen species (ROS). Design: In this prospective study, after removal of seminal plasma, the sperm suspension received no treatment (control), ROS formation by stimulation with phorbol 12-myristate 13-acetate (PMA), antioxidant treatment (with catalase), or PMA stimulus in the presence of catalase. At time zero and after 3 hours of incubation, the percentage of capacitated and oriented spermatozoa and the ROS levels were determined. Setting: Andrology laboratory in a medical research institution. Patient(s): Normal semen was obtained from eight men. Intervention(s): The semen samples were evaluated to determine the effect of ROS production by stimulation with PMA and/or antioxidant treatment (with catalase) on the percentage of capacitated and oriented spermatozoa. Main Outcome Measure(s): The sperm capacitation, chemotaxis and reactive oxygen species were assessed before and after PMA and/or antioxidant treatment. Result(s): Prolonged exposure to high quantities of ROS decrease the sperm chemotactic response, probably because of oxidative damage of the cell. However, this effect may be reduced by the addition of antioxidants like catalase. Conclusion(s): Similar to capacitation, chemotaxis seems to depend on the production of ROS, but in the latter process there may be a critical level of ROS necessary for chemotaxis to occur. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-01-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/57744 Sánchez, Raúl Oscar; Sepúlveda, Carolina; Risopatrón, Jennie; Villegas, Juana; Giojalas, Laura Cecilia; Human sperm chemotaxis depends on critical levels of reactive oxygen species; Elsevier Science Inc; Fertility and Sterility; 93; 1; 1-1-2010; 150-153 0015-0282 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/57744 |
identifier_str_mv |
Sánchez, Raúl Oscar; Sepúlveda, Carolina; Risopatrón, Jennie; Villegas, Juana; Giojalas, Laura Cecilia; Human sperm chemotaxis depends on critical levels of reactive oxygen species; Elsevier Science Inc; Fertility and Sterility; 93; 1; 1-1-2010; 150-153 0015-0282 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0015028208039472 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fertnstert.2008.09.049 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science Inc |
publisher.none.fl_str_mv |
Elsevier Science Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1842269290985684992 |
score |
13.13397 |