Human sperm chemotaxis depends on critical levels of reactive oxygen species

Autores
Sánchez, Raúl Oscar; Sepúlveda, Carolina; Risopatrón, Jennie; Villegas, Juana; Giojalas, Laura Cecilia
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective: To verify whether chemotaxis is in part an oxidative process mediated by reactive oxygen species (ROS). Design: In this prospective study, after removal of seminal plasma, the sperm suspension received no treatment (control), ROS formation by stimulation with phorbol 12-myristate 13-acetate (PMA), antioxidant treatment (with catalase), or PMA stimulus in the presence of catalase. At time zero and after 3 hours of incubation, the percentage of capacitated and oriented spermatozoa and the ROS levels were determined. Setting: Andrology laboratory in a medical research institution. Patient(s): Normal semen was obtained from eight men. Intervention(s): The semen samples were evaluated to determine the effect of ROS production by stimulation with PMA and/or antioxidant treatment (with catalase) on the percentage of capacitated and oriented spermatozoa. Main Outcome Measure(s): The sperm capacitation, chemotaxis and reactive oxygen species were assessed before and after PMA and/or antioxidant treatment. Result(s): Prolonged exposure to high quantities of ROS decrease the sperm chemotactic response, probably because of oxidative damage of the cell. However, this effect may be reduced by the addition of antioxidants like catalase. Conclusion(s): Similar to capacitation, chemotaxis seems to depend on the production of ROS, but in the latter process there may be a critical level of ROS necessary for chemotaxis to occur.
Fil: Sánchez, Raúl Oscar. Universidad de La Frontera; Chile
Fil: Sepúlveda, Carolina. Universidad de La Frontera; Chile
Fil: Risopatrón, Jennie. Universidad de La Frontera; Chile
Fil: Villegas, Juana. Universidad de La Frontera; Chile
Fil: Giojalas, Laura Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; Argentina
Materia
Antioxidant
Chemotaxis
Human Spermatozoa
Ros
Sperm Capacition
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/57744
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/57744
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Human sperm chemotaxis depends on critical levels of reactive oxygen speciesSánchez, Raúl OscarSepúlveda, CarolinaRisopatrón, JennieVillegas, JuanaGiojalas, Laura CeciliaAntioxidantChemotaxisHuman SpermatozoaRosSperm Capacitionhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Objective: To verify whether chemotaxis is in part an oxidative process mediated by reactive oxygen species (ROS). Design: In this prospective study, after removal of seminal plasma, the sperm suspension received no treatment (control), ROS formation by stimulation with phorbol 12-myristate 13-acetate (PMA), antioxidant treatment (with catalase), or PMA stimulus in the presence of catalase. At time zero and after 3 hours of incubation, the percentage of capacitated and oriented spermatozoa and the ROS levels were determined. Setting: Andrology laboratory in a medical research institution. Patient(s): Normal semen was obtained from eight men. Intervention(s): The semen samples were evaluated to determine the effect of ROS production by stimulation with PMA and/or antioxidant treatment (with catalase) on the percentage of capacitated and oriented spermatozoa. Main Outcome Measure(s): The sperm capacitation, chemotaxis and reactive oxygen species were assessed before and after PMA and/or antioxidant treatment. Result(s): Prolonged exposure to high quantities of ROS decrease the sperm chemotactic response, probably because of oxidative damage of the cell. However, this effect may be reduced by the addition of antioxidants like catalase. Conclusion(s): Similar to capacitation, chemotaxis seems to depend on the production of ROS, but in the latter process there may be a critical level of ROS necessary for chemotaxis to occur.Fil: Sánchez, Raúl Oscar. Universidad de La Frontera; ChileFil: Sepúlveda, Carolina. Universidad de La Frontera; ChileFil: Risopatrón, Jennie. Universidad de La Frontera; ChileFil: Villegas, Juana. Universidad de La Frontera; ChileFil: Giojalas, Laura Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; ArgentinaElsevier Science Inc2010-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/57744Sánchez, Raúl Oscar; Sepúlveda, Carolina; Risopatrón, Jennie; Villegas, Juana; Giojalas, Laura Cecilia; Human sperm chemotaxis depends on critical levels of reactive oxygen species; Elsevier Science Inc; Fertility and Sterility; 93; 1; 1-1-2010; 150-1530015-0282CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0015028208039472info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fertnstert.2008.09.049info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:55:44Zoai:ri.conicet.gov.ar:11336/57744instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:55:44.436CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Human sperm chemotaxis depends on critical levels of reactive oxygen species
title Human sperm chemotaxis depends on critical levels of reactive oxygen species
spellingShingle Human sperm chemotaxis depends on critical levels of reactive oxygen species
Sánchez, Raúl Oscar
Antioxidant
Chemotaxis
Human Spermatozoa
Ros
Sperm Capacition
title_short Human sperm chemotaxis depends on critical levels of reactive oxygen species
title_full Human sperm chemotaxis depends on critical levels of reactive oxygen species
title_fullStr Human sperm chemotaxis depends on critical levels of reactive oxygen species
title_full_unstemmed Human sperm chemotaxis depends on critical levels of reactive oxygen species
title_sort Human sperm chemotaxis depends on critical levels of reactive oxygen species
dc.creator.none.fl_str_mv Sánchez, Raúl Oscar
Sepúlveda, Carolina
Risopatrón, Jennie
Villegas, Juana
Giojalas, Laura Cecilia
author Sánchez, Raúl Oscar
author_facet Sánchez, Raúl Oscar
Sepúlveda, Carolina
Risopatrón, Jennie
Villegas, Juana
Giojalas, Laura Cecilia
author_role author
author2 Sepúlveda, Carolina
Risopatrón, Jennie
Villegas, Juana
Giojalas, Laura Cecilia
author2_role author
author
author
author
dc.subject.none.fl_str_mv Antioxidant
Chemotaxis
Human Spermatozoa
Ros
Sperm Capacition
topic Antioxidant
Chemotaxis
Human Spermatozoa
Ros
Sperm Capacition
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Objective: To verify whether chemotaxis is in part an oxidative process mediated by reactive oxygen species (ROS). Design: In this prospective study, after removal of seminal plasma, the sperm suspension received no treatment (control), ROS formation by stimulation with phorbol 12-myristate 13-acetate (PMA), antioxidant treatment (with catalase), or PMA stimulus in the presence of catalase. At time zero and after 3 hours of incubation, the percentage of capacitated and oriented spermatozoa and the ROS levels were determined. Setting: Andrology laboratory in a medical research institution. Patient(s): Normal semen was obtained from eight men. Intervention(s): The semen samples were evaluated to determine the effect of ROS production by stimulation with PMA and/or antioxidant treatment (with catalase) on the percentage of capacitated and oriented spermatozoa. Main Outcome Measure(s): The sperm capacitation, chemotaxis and reactive oxygen species were assessed before and after PMA and/or antioxidant treatment. Result(s): Prolonged exposure to high quantities of ROS decrease the sperm chemotactic response, probably because of oxidative damage of the cell. However, this effect may be reduced by the addition of antioxidants like catalase. Conclusion(s): Similar to capacitation, chemotaxis seems to depend on the production of ROS, but in the latter process there may be a critical level of ROS necessary for chemotaxis to occur.
Fil: Sánchez, Raúl Oscar. Universidad de La Frontera; Chile
Fil: Sepúlveda, Carolina. Universidad de La Frontera; Chile
Fil: Risopatrón, Jennie. Universidad de La Frontera; Chile
Fil: Villegas, Juana. Universidad de La Frontera; Chile
Fil: Giojalas, Laura Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; Argentina
description Objective: To verify whether chemotaxis is in part an oxidative process mediated by reactive oxygen species (ROS). Design: In this prospective study, after removal of seminal plasma, the sperm suspension received no treatment (control), ROS formation by stimulation with phorbol 12-myristate 13-acetate (PMA), antioxidant treatment (with catalase), or PMA stimulus in the presence of catalase. At time zero and after 3 hours of incubation, the percentage of capacitated and oriented spermatozoa and the ROS levels were determined. Setting: Andrology laboratory in a medical research institution. Patient(s): Normal semen was obtained from eight men. Intervention(s): The semen samples were evaluated to determine the effect of ROS production by stimulation with PMA and/or antioxidant treatment (with catalase) on the percentage of capacitated and oriented spermatozoa. Main Outcome Measure(s): The sperm capacitation, chemotaxis and reactive oxygen species were assessed before and after PMA and/or antioxidant treatment. Result(s): Prolonged exposure to high quantities of ROS decrease the sperm chemotactic response, probably because of oxidative damage of the cell. However, this effect may be reduced by the addition of antioxidants like catalase. Conclusion(s): Similar to capacitation, chemotaxis seems to depend on the production of ROS, but in the latter process there may be a critical level of ROS necessary for chemotaxis to occur.
publishDate 2010
dc.date.none.fl_str_mv 2010-01-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/57744
Sánchez, Raúl Oscar; Sepúlveda, Carolina; Risopatrón, Jennie; Villegas, Juana; Giojalas, Laura Cecilia; Human sperm chemotaxis depends on critical levels of reactive oxygen species; Elsevier Science Inc; Fertility and Sterility; 93; 1; 1-1-2010; 150-153
0015-0282
CONICET Digital
CONICET
url http://hdl.handle.net/11336/57744
identifier_str_mv Sánchez, Raúl Oscar; Sepúlveda, Carolina; Risopatrón, Jennie; Villegas, Juana; Giojalas, Laura Cecilia; Human sperm chemotaxis depends on critical levels of reactive oxygen species; Elsevier Science Inc; Fertility and Sterility; 93; 1; 1-1-2010; 150-153
0015-0282
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0015028208039472
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fertnstert.2008.09.049
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc
publisher.none.fl_str_mv Elsevier Science Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613678507229184
score 13.070432

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