A role for the chemokine receptor CCR6 in mammalian sperm motility and chemotaxis

Autores
Caballero Campo, Pedro; Buffone, Mariano Gabriel; Benencia, Fabián; Conejo García, José R.; Rinaudo, Paolo F.; Gerton, George L.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Although recent evidence indicates that several chemokines and defensins, well-known as inflammatory mediators, are expressed in the male and female reproductive tracts, the location and functional significance of chemokine networks in sperm physiology and sperm reproductive tract interactions are poorly understood. To address this deficiency in our knowledge, we examined the expression and function in sperm of CCR6, a receptor common to several chemoattractant peptides, and screened several reproductive tract fluids for the presence of specific ligands. CCR6 protein is present in mouse and human sperm and mainly localized in the sperm tail with other minor patterns in sperm from mice (neck and acrosomal region) and men (neck and midpiece regions). As expected from the protein immunoblotting and immunofluorescence results, mouse Ccr6 mRNA is expressed in the testis. Furthermore, the Defb29 mRNA encoding the CCR6 ligand, β-defensin DEFB29, is expressed at high levels in the epididymis. As determined by protein chip analysis, several chemokines (including some that act through CCR6, such as CCL20/MIP-3α (formerly macrophage inflammatory protein 3α) and protein hormones were present in human follicular fluid, endometrial secretions, and seminal plasma. In functional chemotaxis assays, capacitated human sperm exhibited a directional movement towards CCL20, and displayed modifications in motility parameters. Our data indicate that chemokine ligand/receptor interactions in the male and female genital tracts promote sperm motility and chemotaxis under non-inflammatory conditions. Therefore, some of the physiological reactions mediated by CCR6 ligands in male reproduction extend beyond a pro-inflammatory response and might find application in clinical reproduction and/or contraception.
Fil: Caballero Campo, Pedro. Clínica Tambre. Unidad de Reproducción Humana; España. University of California; Estados Unidos
Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Benencia, Fabián. Ohio University; Estados Unidos
Fil: Conejo García, José R.. The Wistar Institute; Estados Unidos
Fil: Rinaudo, Paolo F.. University of California; Estados Unidos
Fil: Gerton, George L.. University of Pennsylvania; Estados Unidos
Materia
SPERM
CCR6
CHEMOTAXIS
SPERM MOTILITY
CHEMOKINE CCL20
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/19277
Acceder
id CONICETDig_12c690c7223fd546faea13447d0c6ee3
oai_identifier_str oai:ri.conicet.gov.ar:11336/19277
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A role for the chemokine receptor CCR6 in mammalian sperm motility and chemotaxisCaballero Campo, PedroBuffone, Mariano GabrielBenencia, FabiánConejo García, José R.Rinaudo, Paolo F.Gerton, George L.SPERMCCR6CHEMOTAXISSPERM MOTILITYCHEMOKINE CCL20https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Although recent evidence indicates that several chemokines and defensins, well-known as inflammatory mediators, are expressed in the male and female reproductive tracts, the location and functional significance of chemokine networks in sperm physiology and sperm reproductive tract interactions are poorly understood. To address this deficiency in our knowledge, we examined the expression and function in sperm of CCR6, a receptor common to several chemoattractant peptides, and screened several reproductive tract fluids for the presence of specific ligands. CCR6 protein is present in mouse and human sperm and mainly localized in the sperm tail with other minor patterns in sperm from mice (neck and acrosomal region) and men (neck and midpiece regions). As expected from the protein immunoblotting and immunofluorescence results, mouse Ccr6 mRNA is expressed in the testis. Furthermore, the Defb29 mRNA encoding the CCR6 ligand, β-defensin DEFB29, is expressed at high levels in the epididymis. As determined by protein chip analysis, several chemokines (including some that act through CCR6, such as CCL20/MIP-3α (formerly macrophage inflammatory protein 3α) and protein hormones were present in human follicular fluid, endometrial secretions, and seminal plasma. In functional chemotaxis assays, capacitated human sperm exhibited a directional movement towards CCL20, and displayed modifications in motility parameters. Our data indicate that chemokine ligand/receptor interactions in the male and female genital tracts promote sperm motility and chemotaxis under non-inflammatory conditions. Therefore, some of the physiological reactions mediated by CCR6 ligands in male reproduction extend beyond a pro-inflammatory response and might find application in clinical reproduction and/or contraception.Fil: Caballero Campo, Pedro. Clínica Tambre. Unidad de Reproducción Humana; España. University of California; Estados UnidosFil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Benencia, Fabián. Ohio University; Estados UnidosFil: Conejo García, José R.. The Wistar Institute; Estados UnidosFil: Rinaudo, Paolo F.. University of California; Estados UnidosFil: Gerton, George L.. University of Pennsylvania; Estados UnidosWiley2014-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/19277Caballero Campo, Pedro; Buffone, Mariano Gabriel; Benencia, Fabián; Conejo García, José R.; Rinaudo, Paolo F.; et al.; A role for the chemokine receptor CCR6 in mammalian sperm motility and chemotaxis; Wiley; Journal Of Cellular Physiology; 229; 1; 1-1-2014; 68-780021-95411097-4652enginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jcp.24418/abstractinfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3807638/info:eu-repo/semantics/altIdentifier/doi/10.1002/jcp.24418info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:59:54Zoai:ri.conicet.gov.ar:11336/19277instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:59:54.513CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A role for the chemokine receptor CCR6 in mammalian sperm motility and chemotaxis
title A role for the chemokine receptor CCR6 in mammalian sperm motility and chemotaxis
spellingShingle A role for the chemokine receptor CCR6 in mammalian sperm motility and chemotaxis
Caballero Campo, Pedro
SPERM
CCR6
CHEMOTAXIS
SPERM MOTILITY
CHEMOKINE CCL20
title_short A role for the chemokine receptor CCR6 in mammalian sperm motility and chemotaxis
title_full A role for the chemokine receptor CCR6 in mammalian sperm motility and chemotaxis
title_fullStr A role for the chemokine receptor CCR6 in mammalian sperm motility and chemotaxis
title_full_unstemmed A role for the chemokine receptor CCR6 in mammalian sperm motility and chemotaxis
title_sort A role for the chemokine receptor CCR6 in mammalian sperm motility and chemotaxis
dc.creator.none.fl_str_mv Caballero Campo, Pedro
Buffone, Mariano Gabriel
Benencia, Fabián
Conejo García, José R.
Rinaudo, Paolo F.
Gerton, George L.
author Caballero Campo, Pedro
author_facet Caballero Campo, Pedro
Buffone, Mariano Gabriel
Benencia, Fabián
Conejo García, José R.
Rinaudo, Paolo F.
Gerton, George L.
author_role author
author2 Buffone, Mariano Gabriel
Benencia, Fabián
Conejo García, José R.
Rinaudo, Paolo F.
Gerton, George L.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv SPERM
CCR6
CHEMOTAXIS
SPERM MOTILITY
CHEMOKINE CCL20
topic SPERM
CCR6
CHEMOTAXIS
SPERM MOTILITY
CHEMOKINE CCL20
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Although recent evidence indicates that several chemokines and defensins, well-known as inflammatory mediators, are expressed in the male and female reproductive tracts, the location and functional significance of chemokine networks in sperm physiology and sperm reproductive tract interactions are poorly understood. To address this deficiency in our knowledge, we examined the expression and function in sperm of CCR6, a receptor common to several chemoattractant peptides, and screened several reproductive tract fluids for the presence of specific ligands. CCR6 protein is present in mouse and human sperm and mainly localized in the sperm tail with other minor patterns in sperm from mice (neck and acrosomal region) and men (neck and midpiece regions). As expected from the protein immunoblotting and immunofluorescence results, mouse Ccr6 mRNA is expressed in the testis. Furthermore, the Defb29 mRNA encoding the CCR6 ligand, β-defensin DEFB29, is expressed at high levels in the epididymis. As determined by protein chip analysis, several chemokines (including some that act through CCR6, such as CCL20/MIP-3α (formerly macrophage inflammatory protein 3α) and protein hormones were present in human follicular fluid, endometrial secretions, and seminal plasma. In functional chemotaxis assays, capacitated human sperm exhibited a directional movement towards CCL20, and displayed modifications in motility parameters. Our data indicate that chemokine ligand/receptor interactions in the male and female genital tracts promote sperm motility and chemotaxis under non-inflammatory conditions. Therefore, some of the physiological reactions mediated by CCR6 ligands in male reproduction extend beyond a pro-inflammatory response and might find application in clinical reproduction and/or contraception.
Fil: Caballero Campo, Pedro. Clínica Tambre. Unidad de Reproducción Humana; España. University of California; Estados Unidos
Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Benencia, Fabián. Ohio University; Estados Unidos
Fil: Conejo García, José R.. The Wistar Institute; Estados Unidos
Fil: Rinaudo, Paolo F.. University of California; Estados Unidos
Fil: Gerton, George L.. University of Pennsylvania; Estados Unidos
description Although recent evidence indicates that several chemokines and defensins, well-known as inflammatory mediators, are expressed in the male and female reproductive tracts, the location and functional significance of chemokine networks in sperm physiology and sperm reproductive tract interactions are poorly understood. To address this deficiency in our knowledge, we examined the expression and function in sperm of CCR6, a receptor common to several chemoattractant peptides, and screened several reproductive tract fluids for the presence of specific ligands. CCR6 protein is present in mouse and human sperm and mainly localized in the sperm tail with other minor patterns in sperm from mice (neck and acrosomal region) and men (neck and midpiece regions). As expected from the protein immunoblotting and immunofluorescence results, mouse Ccr6 mRNA is expressed in the testis. Furthermore, the Defb29 mRNA encoding the CCR6 ligand, β-defensin DEFB29, is expressed at high levels in the epididymis. As determined by protein chip analysis, several chemokines (including some that act through CCR6, such as CCL20/MIP-3α (formerly macrophage inflammatory protein 3α) and protein hormones were present in human follicular fluid, endometrial secretions, and seminal plasma. In functional chemotaxis assays, capacitated human sperm exhibited a directional movement towards CCL20, and displayed modifications in motility parameters. Our data indicate that chemokine ligand/receptor interactions in the male and female genital tracts promote sperm motility and chemotaxis under non-inflammatory conditions. Therefore, some of the physiological reactions mediated by CCR6 ligands in male reproduction extend beyond a pro-inflammatory response and might find application in clinical reproduction and/or contraception.
publishDate 2014
dc.date.none.fl_str_mv 2014-01-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/19277
Caballero Campo, Pedro; Buffone, Mariano Gabriel; Benencia, Fabián; Conejo García, José R.; Rinaudo, Paolo F.; et al.; A role for the chemokine receptor CCR6 in mammalian sperm motility and chemotaxis; Wiley; Journal Of Cellular Physiology; 229; 1; 1-1-2014; 68-78
0021-9541
1097-4652
url http://hdl.handle.net/11336/19277
identifier_str_mv Caballero Campo, Pedro; Buffone, Mariano Gabriel; Benencia, Fabián; Conejo García, José R.; Rinaudo, Paolo F.; et al.; A role for the chemokine receptor CCR6 in mammalian sperm motility and chemotaxis; Wiley; Journal Of Cellular Physiology; 229; 1; 1-1-2014; 68-78
0021-9541
1097-4652
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jcp.24418/abstract
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3807638/
info:eu-repo/semantics/altIdentifier/doi/10.1002/jcp.24418
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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