Human sperm chemotaxis: Both the oocyte and its surrounding cumulus cells secrete sperm chemoattractants

Autores
Sun, Fei; Bahat, Anat; Gakamsky, Anna; Girsh, Eliezer; Katz, Nathan; Giojalas, Laura Cecilia; Tur-Kaspa, Ilan; Eisenbach, Michael
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Human sperm chemotaxis to pre-ovulatory follicular fluid is well established in vitro. However, it is not known whether the female's oocyte-cumulus complex secretes sperm chemoattractants subsequent to ovulation (for enabling sperm chemotaxis within the Fallopian tube) and, if so, which of these cell types - the oocyte or the cumulus oophorus - is the physiological origin of the secreted chemoattractant. Methods: By employing a directionality-based chemotaxis assay, we examined whether media conditioned with either individual, mature (metaphase II) human oocytes or the surrounding cumulus cells attract human sperm by chemotaxis. Results: We observed sperm chemotaxis to each of these media, suggesting that both the oocyte and the cumulus cells secrete sperm chemoattractants. Conclusions: These observations suggest that sperm chemoattractants are secreted not only prior to ovulation within the follicle, as earlier studies have demonstrated, but also after oocyte maturation outside the follicle, and that there are two chemoattractant origins: the mature oocyte and the surrounding cumulus cells.
Fil: Sun, Fei. Weizmann Institute of Science. Department of Biological Chemistry; Israel
Fil: Bahat, Anat. Weizmann Institute of Science. Department of Biological Chemistry; Israel
Fil: Gakamsky, Anna. Weizmann Institute of Science. Department of Biological Chemistry; Israel
Fil: Girsh, Eliezer. Ben Gurion University of the Negev; Israel
Fil: Katz, Nathan. Ben Gurion University of the Negev; Israel
Fil: Giojalas, Laura Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; Argentina
Fil: Tur-Kaspa, Ilan. Ben Gurion University of the Negev; Israel
Fil: Eisenbach, Michael. Weizmann Institute of Science. Department of Biological Chemistry; Israel
Materia
Chemotaxis
Cumulus Cells
Oocyte-Conditioned Medium
Ovum
Sperm Chemoattractant
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/57580
Acceder
id CONICETDig_fe56bf88aa81e2e437c2583435ca0d77
oai_identifier_str oai:ri.conicet.gov.ar:11336/57580
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Human sperm chemotaxis: Both the oocyte and its surrounding cumulus cells secrete sperm chemoattractantsSun, FeiBahat, AnatGakamsky, AnnaGirsh, EliezerKatz, NathanGiojalas, Laura CeciliaTur-Kaspa, IlanEisenbach, MichaelChemotaxisCumulus CellsOocyte-Conditioned MediumOvumSperm Chemoattractanthttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: Human sperm chemotaxis to pre-ovulatory follicular fluid is well established in vitro. However, it is not known whether the female's oocyte-cumulus complex secretes sperm chemoattractants subsequent to ovulation (for enabling sperm chemotaxis within the Fallopian tube) and, if so, which of these cell types - the oocyte or the cumulus oophorus - is the physiological origin of the secreted chemoattractant. Methods: By employing a directionality-based chemotaxis assay, we examined whether media conditioned with either individual, mature (metaphase II) human oocytes or the surrounding cumulus cells attract human sperm by chemotaxis. Results: We observed sperm chemotaxis to each of these media, suggesting that both the oocyte and the cumulus cells secrete sperm chemoattractants. Conclusions: These observations suggest that sperm chemoattractants are secreted not only prior to ovulation within the follicle, as earlier studies have demonstrated, but also after oocyte maturation outside the follicle, and that there are two chemoattractant origins: the mature oocyte and the surrounding cumulus cells.Fil: Sun, Fei. Weizmann Institute of Science. Department of Biological Chemistry; IsraelFil: Bahat, Anat. Weizmann Institute of Science. Department of Biological Chemistry; IsraelFil: Gakamsky, Anna. Weizmann Institute of Science. Department of Biological Chemistry; IsraelFil: Girsh, Eliezer. Ben Gurion University of the Negev; IsraelFil: Katz, Nathan. Ben Gurion University of the Negev; IsraelFil: Giojalas, Laura Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; ArgentinaFil: Tur-Kaspa, Ilan. Ben Gurion University of the Negev; IsraelFil: Eisenbach, Michael. Weizmann Institute of Science. Department of Biological Chemistry; IsraelOxford University Press2005-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/57580Sun, Fei; Bahat, Anat; Gakamsky, Anna; Girsh, Eliezer; Katz, Nathan; et al.; Human sperm chemotaxis: Both the oocyte and its surrounding cumulus cells secrete sperm chemoattractants; Oxford University Press; Human Reproduction; 20; 3; 3-2005; 761-7670268-11611460-2350CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/humrep/article/20/3/761/2356534info:eu-repo/semantics/altIdentifier/doi/10.1093/humrep/deh657info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:04:54Zoai:ri.conicet.gov.ar:11336/57580instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:04:54.523CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Human sperm chemotaxis: Both the oocyte and its surrounding cumulus cells secrete sperm chemoattractants
title Human sperm chemotaxis: Both the oocyte and its surrounding cumulus cells secrete sperm chemoattractants
spellingShingle Human sperm chemotaxis: Both the oocyte and its surrounding cumulus cells secrete sperm chemoattractants
Sun, Fei
Chemotaxis
Cumulus Cells
Oocyte-Conditioned Medium
Ovum
Sperm Chemoattractant
title_short Human sperm chemotaxis: Both the oocyte and its surrounding cumulus cells secrete sperm chemoattractants
title_full Human sperm chemotaxis: Both the oocyte and its surrounding cumulus cells secrete sperm chemoattractants
title_fullStr Human sperm chemotaxis: Both the oocyte and its surrounding cumulus cells secrete sperm chemoattractants
title_full_unstemmed Human sperm chemotaxis: Both the oocyte and its surrounding cumulus cells secrete sperm chemoattractants
title_sort Human sperm chemotaxis: Both the oocyte and its surrounding cumulus cells secrete sperm chemoattractants
dc.creator.none.fl_str_mv Sun, Fei
Bahat, Anat
Gakamsky, Anna
Girsh, Eliezer
Katz, Nathan
Giojalas, Laura Cecilia
Tur-Kaspa, Ilan
Eisenbach, Michael
author Sun, Fei
author_facet Sun, Fei
Bahat, Anat
Gakamsky, Anna
Girsh, Eliezer
Katz, Nathan
Giojalas, Laura Cecilia
Tur-Kaspa, Ilan
Eisenbach, Michael
author_role author
author2 Bahat, Anat
Gakamsky, Anna
Girsh, Eliezer
Katz, Nathan
Giojalas, Laura Cecilia
Tur-Kaspa, Ilan
Eisenbach, Michael
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Chemotaxis
Cumulus Cells
Oocyte-Conditioned Medium
Ovum
Sperm Chemoattractant
topic Chemotaxis
Cumulus Cells
Oocyte-Conditioned Medium
Ovum
Sperm Chemoattractant
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: Human sperm chemotaxis to pre-ovulatory follicular fluid is well established in vitro. However, it is not known whether the female's oocyte-cumulus complex secretes sperm chemoattractants subsequent to ovulation (for enabling sperm chemotaxis within the Fallopian tube) and, if so, which of these cell types - the oocyte or the cumulus oophorus - is the physiological origin of the secreted chemoattractant. Methods: By employing a directionality-based chemotaxis assay, we examined whether media conditioned with either individual, mature (metaphase II) human oocytes or the surrounding cumulus cells attract human sperm by chemotaxis. Results: We observed sperm chemotaxis to each of these media, suggesting that both the oocyte and the cumulus cells secrete sperm chemoattractants. Conclusions: These observations suggest that sperm chemoattractants are secreted not only prior to ovulation within the follicle, as earlier studies have demonstrated, but also after oocyte maturation outside the follicle, and that there are two chemoattractant origins: the mature oocyte and the surrounding cumulus cells.
Fil: Sun, Fei. Weizmann Institute of Science. Department of Biological Chemistry; Israel
Fil: Bahat, Anat. Weizmann Institute of Science. Department of Biological Chemistry; Israel
Fil: Gakamsky, Anna. Weizmann Institute of Science. Department of Biological Chemistry; Israel
Fil: Girsh, Eliezer. Ben Gurion University of the Negev; Israel
Fil: Katz, Nathan. Ben Gurion University of the Negev; Israel
Fil: Giojalas, Laura Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; Argentina
Fil: Tur-Kaspa, Ilan. Ben Gurion University of the Negev; Israel
Fil: Eisenbach, Michael. Weizmann Institute of Science. Department of Biological Chemistry; Israel
description Background: Human sperm chemotaxis to pre-ovulatory follicular fluid is well established in vitro. However, it is not known whether the female's oocyte-cumulus complex secretes sperm chemoattractants subsequent to ovulation (for enabling sperm chemotaxis within the Fallopian tube) and, if so, which of these cell types - the oocyte or the cumulus oophorus - is the physiological origin of the secreted chemoattractant. Methods: By employing a directionality-based chemotaxis assay, we examined whether media conditioned with either individual, mature (metaphase II) human oocytes or the surrounding cumulus cells attract human sperm by chemotaxis. Results: We observed sperm chemotaxis to each of these media, suggesting that both the oocyte and the cumulus cells secrete sperm chemoattractants. Conclusions: These observations suggest that sperm chemoattractants are secreted not only prior to ovulation within the follicle, as earlier studies have demonstrated, but also after oocyte maturation outside the follicle, and that there are two chemoattractant origins: the mature oocyte and the surrounding cumulus cells.
publishDate 2005
dc.date.none.fl_str_mv 2005-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/57580
Sun, Fei; Bahat, Anat; Gakamsky, Anna; Girsh, Eliezer; Katz, Nathan; et al.; Human sperm chemotaxis: Both the oocyte and its surrounding cumulus cells secrete sperm chemoattractants; Oxford University Press; Human Reproduction; 20; 3; 3-2005; 761-767
0268-1161
1460-2350
CONICET Digital
CONICET
url http://hdl.handle.net/11336/57580
identifier_str_mv Sun, Fei; Bahat, Anat; Gakamsky, Anna; Girsh, Eliezer; Katz, Nathan; et al.; Human sperm chemotaxis: Both the oocyte and its surrounding cumulus cells secrete sperm chemoattractants; Oxford University Press; Human Reproduction; 20; 3; 3-2005; 761-767
0268-1161
1460-2350
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/humrep/article/20/3/761/2356534
info:eu-repo/semantics/altIdentifier/doi/10.1093/humrep/deh657
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269880775081984
score 13.13397

Publicaciones similares