Molecular mechanism for human sperm chemotaxis mediated by progesterone

Autores
Teves, Maria Eugenia; Guidobaldi, Héctor Alejandro; Uñates, Diego Rafael; Sánchez, Raúl Oscar; Miska, Werner; Publicover, Stephen J.; Morales Garcia, Aduén A.; Giojalas, Laura Cecilia
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Sperm chemotaxis is a chemical guiding mechanism that may orient spermatozoa to the egg surface. A picomolar concentration gradient of Progesterone (P), the main steroidal component secreted by the cumulus cells that surround the egg, attracts human spermatozoa. In order to elucidate the molecular mechanism of sperm chemotaxis mediated by P, we combine the application of different strategies: pharmacological inhibition of signaling molecules, measurements of the concentrations of second messengers and activation of the chemotactic signaling. Our data implicate a number of classic signal transduction pathways in the response and provide a model for the sequence of events, where the tmAC-cAMP-PKA pathway is activated first, followed by protein tyrosine phosphorylation (equatorial band and flagellum) and calcium mobilization (through IP3R and SOC channels), whereas the sGC-cGMP-PKG cascade, is activated later. These events lead to sperm orientation towards the source of the chemoattractant. The finding proposes a molecular mechanism which contributes to the understanding of the signal transduction pathway that takes place in a physiological process as chemotaxis. © 2009 Teves et al.
Fil: Teves, Maria Eugenia. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; Argentina
Fil: Guidobaldi, Héctor Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Uñates, Diego Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina
Fil: Sánchez, Raúl Oscar. Universidad de La Frontera; Chile
Fil: Miska, Werner. Justus Liebig University; Alemania
Fil: Publicover, Stephen J.. University of Birmingham. School of Biosciences; Reino Unido
Fil: Morales Garcia, Aduén A.. University of Birmingham. School of Biosciences; Reino Unido
Fil: Giojalas, Laura Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Materia
CHEMOTAXIS
MOLECULAR MECHANISM
PROGESTERONE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/59690
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/59690
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Molecular mechanism for human sperm chemotaxis mediated by progesteroneTeves, Maria EugeniaGuidobaldi, Héctor AlejandroUñates, Diego RafaelSánchez, Raúl OscarMiska, WernerPublicover, Stephen J.Morales Garcia, Aduén A.Giojalas, Laura CeciliaCHEMOTAXISMOLECULAR MECHANISMPROGESTERONEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Sperm chemotaxis is a chemical guiding mechanism that may orient spermatozoa to the egg surface. A picomolar concentration gradient of Progesterone (P), the main steroidal component secreted by the cumulus cells that surround the egg, attracts human spermatozoa. In order to elucidate the molecular mechanism of sperm chemotaxis mediated by P, we combine the application of different strategies: pharmacological inhibition of signaling molecules, measurements of the concentrations of second messengers and activation of the chemotactic signaling. Our data implicate a number of classic signal transduction pathways in the response and provide a model for the sequence of events, where the tmAC-cAMP-PKA pathway is activated first, followed by protein tyrosine phosphorylation (equatorial band and flagellum) and calcium mobilization (through IP3R and SOC channels), whereas the sGC-cGMP-PKG cascade, is activated later. These events lead to sperm orientation towards the source of the chemoattractant. The finding proposes a molecular mechanism which contributes to the understanding of the signal transduction pathway that takes place in a physiological process as chemotaxis. © 2009 Teves et al.Fil: Teves, Maria Eugenia. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; ArgentinaFil: Guidobaldi, Héctor Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Uñates, Diego Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; ArgentinaFil: Sánchez, Raúl Oscar. Universidad de La Frontera; ChileFil: Miska, Werner. Justus Liebig University; AlemaniaFil: Publicover, Stephen J.. University of Birmingham. School of Biosciences; Reino UnidoFil: Morales Garcia, Aduén A.. University of Birmingham. School of Biosciences; Reino UnidoFil: Giojalas, Laura Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaPublic Library of Science2009-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/59690Teves, Maria Eugenia; Guidobaldi, Héctor Alejandro; Uñates, Diego Rafael; Sánchez, Raúl Oscar; Miska, Werner; et al.; Molecular mechanism for human sperm chemotaxis mediated by progesterone; Public Library of Science; Plos One; 4; 12; 12-20091932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0008211info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0008211info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:58:24Zoai:ri.conicet.gov.ar:11336/59690instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:58:24.756CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular mechanism for human sperm chemotaxis mediated by progesterone
title Molecular mechanism for human sperm chemotaxis mediated by progesterone
spellingShingle Molecular mechanism for human sperm chemotaxis mediated by progesterone
Teves, Maria Eugenia
CHEMOTAXIS
MOLECULAR MECHANISM
PROGESTERONE
title_short Molecular mechanism for human sperm chemotaxis mediated by progesterone
title_full Molecular mechanism for human sperm chemotaxis mediated by progesterone
title_fullStr Molecular mechanism for human sperm chemotaxis mediated by progesterone
title_full_unstemmed Molecular mechanism for human sperm chemotaxis mediated by progesterone
title_sort Molecular mechanism for human sperm chemotaxis mediated by progesterone
dc.creator.none.fl_str_mv Teves, Maria Eugenia
Guidobaldi, Héctor Alejandro
Uñates, Diego Rafael
Sánchez, Raúl Oscar
Miska, Werner
Publicover, Stephen J.
Morales Garcia, Aduén A.
Giojalas, Laura Cecilia
author Teves, Maria Eugenia
author_facet Teves, Maria Eugenia
Guidobaldi, Héctor Alejandro
Uñates, Diego Rafael
Sánchez, Raúl Oscar
Miska, Werner
Publicover, Stephen J.
Morales Garcia, Aduén A.
Giojalas, Laura Cecilia
author_role author
author2 Guidobaldi, Héctor Alejandro
Uñates, Diego Rafael
Sánchez, Raúl Oscar
Miska, Werner
Publicover, Stephen J.
Morales Garcia, Aduén A.
Giojalas, Laura Cecilia
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CHEMOTAXIS
MOLECULAR MECHANISM
PROGESTERONE
topic CHEMOTAXIS
MOLECULAR MECHANISM
PROGESTERONE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Sperm chemotaxis is a chemical guiding mechanism that may orient spermatozoa to the egg surface. A picomolar concentration gradient of Progesterone (P), the main steroidal component secreted by the cumulus cells that surround the egg, attracts human spermatozoa. In order to elucidate the molecular mechanism of sperm chemotaxis mediated by P, we combine the application of different strategies: pharmacological inhibition of signaling molecules, measurements of the concentrations of second messengers and activation of the chemotactic signaling. Our data implicate a number of classic signal transduction pathways in the response and provide a model for the sequence of events, where the tmAC-cAMP-PKA pathway is activated first, followed by protein tyrosine phosphorylation (equatorial band and flagellum) and calcium mobilization (through IP3R and SOC channels), whereas the sGC-cGMP-PKG cascade, is activated later. These events lead to sperm orientation towards the source of the chemoattractant. The finding proposes a molecular mechanism which contributes to the understanding of the signal transduction pathway that takes place in a physiological process as chemotaxis. © 2009 Teves et al.
Fil: Teves, Maria Eugenia. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; Argentina
Fil: Guidobaldi, Héctor Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Uñates, Diego Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina
Fil: Sánchez, Raúl Oscar. Universidad de La Frontera; Chile
Fil: Miska, Werner. Justus Liebig University; Alemania
Fil: Publicover, Stephen J.. University of Birmingham. School of Biosciences; Reino Unido
Fil: Morales Garcia, Aduén A.. University of Birmingham. School of Biosciences; Reino Unido
Fil: Giojalas, Laura Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
description Sperm chemotaxis is a chemical guiding mechanism that may orient spermatozoa to the egg surface. A picomolar concentration gradient of Progesterone (P), the main steroidal component secreted by the cumulus cells that surround the egg, attracts human spermatozoa. In order to elucidate the molecular mechanism of sperm chemotaxis mediated by P, we combine the application of different strategies: pharmacological inhibition of signaling molecules, measurements of the concentrations of second messengers and activation of the chemotactic signaling. Our data implicate a number of classic signal transduction pathways in the response and provide a model for the sequence of events, where the tmAC-cAMP-PKA pathway is activated first, followed by protein tyrosine phosphorylation (equatorial band and flagellum) and calcium mobilization (through IP3R and SOC channels), whereas the sGC-cGMP-PKG cascade, is activated later. These events lead to sperm orientation towards the source of the chemoattractant. The finding proposes a molecular mechanism which contributes to the understanding of the signal transduction pathway that takes place in a physiological process as chemotaxis. © 2009 Teves et al.
publishDate 2009
dc.date.none.fl_str_mv 2009-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/59690
Teves, Maria Eugenia; Guidobaldi, Héctor Alejandro; Uñates, Diego Rafael; Sánchez, Raúl Oscar; Miska, Werner; et al.; Molecular mechanism for human sperm chemotaxis mediated by progesterone; Public Library of Science; Plos One; 4; 12; 12-2009
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/59690
identifier_str_mv Teves, Maria Eugenia; Guidobaldi, Héctor Alejandro; Uñates, Diego Rafael; Sánchez, Raúl Oscar; Miska, Werner; et al.; Molecular mechanism for human sperm chemotaxis mediated by progesterone; Public Library of Science; Plos One; 4; 12; 12-2009
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0008211
info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0008211
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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