Caffeine determination by flow injection analysis employing Bovine Serum Albumin as a fluorophore

Autores
Alesso, Magdalena; Fernandez, Liliana Patricia
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A new methodology for caffeine determination was developed based on the quenching effect on fluorescent emission of the molecule of Bovine Serum Albumin at λem = 338 nm (λex = 280). A flow injection disposition was designed improving significantly the sampling rate to 60 samples/h using potassium dihydrophosphate 5 · 10-3 mol L-1 buffer (pH 6.8) as carrier and flow rate of 1.5 mL min-1. The experimental and instrumental conditions that influence on analytical quality parameters were systematically investigated, as consider: buffer nature and concentration, fluorophore nature and concentration, and carrier flow rate. The proposal is simple, fast, inexpensive and precise, with a linear range from 6.68 · 10-6 to 4.0 · 10-3 mol L-1 and SD of 0.0668, under optimized conditions. Methodology sensibility and selectivity allowed a variety of sample analyses. It was successfully applied to caffeine quantification in energy drinks, dietary supplements and sliming infusion samples without previous treatment.
Fil: Alesso, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Materia
Bovine Serum Albumin
Caffeine Determination
Energy Supplements
Flow Injection Analysis
Molecular Fluorescence
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/60470
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Caffeine determination by flow injection analysis employing Bovine Serum Albumin as a fluorophoreAlesso, MagdalenaFernandez, Liliana PatriciaBovine Serum AlbuminCaffeine DeterminationEnergy SupplementsFlow Injection AnalysisMolecular Fluorescencehttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A new methodology for caffeine determination was developed based on the quenching effect on fluorescent emission of the molecule of Bovine Serum Albumin at λem = 338 nm (λex = 280). A flow injection disposition was designed improving significantly the sampling rate to 60 samples/h using potassium dihydrophosphate 5 · 10-3 mol L-1 buffer (pH 6.8) as carrier and flow rate of 1.5 mL min-1. The experimental and instrumental conditions that influence on analytical quality parameters were systematically investigated, as consider: buffer nature and concentration, fluorophore nature and concentration, and carrier flow rate. The proposal is simple, fast, inexpensive and precise, with a linear range from 6.68 · 10-6 to 4.0 · 10-3 mol L-1 and SD of 0.0668, under optimized conditions. Methodology sensibility and selectivity allowed a variety of sample analyses. It was successfully applied to caffeine quantification in energy drinks, dietary supplements and sliming infusion samples without previous treatment.Fil: Alesso, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaElsevier Science2016-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/60470Alesso, Magdalena; Fernandez, Liliana Patricia; Caffeine determination by flow injection analysis employing Bovine Serum Albumin as a fluorophore; Elsevier Science; Microchemical Journal; 127; 7-2016; 165-1690026-265XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2016.03.004info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0026265X16300017info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:59Zoai:ri.conicet.gov.ar:11336/60470instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:59.585CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Caffeine determination by flow injection analysis employing Bovine Serum Albumin as a fluorophore
title Caffeine determination by flow injection analysis employing Bovine Serum Albumin as a fluorophore
spellingShingle Caffeine determination by flow injection analysis employing Bovine Serum Albumin as a fluorophore
Alesso, Magdalena
Bovine Serum Albumin
Caffeine Determination
Energy Supplements
Flow Injection Analysis
Molecular Fluorescence
title_short Caffeine determination by flow injection analysis employing Bovine Serum Albumin as a fluorophore
title_full Caffeine determination by flow injection analysis employing Bovine Serum Albumin as a fluorophore
title_fullStr Caffeine determination by flow injection analysis employing Bovine Serum Albumin as a fluorophore
title_full_unstemmed Caffeine determination by flow injection analysis employing Bovine Serum Albumin as a fluorophore
title_sort Caffeine determination by flow injection analysis employing Bovine Serum Albumin as a fluorophore
dc.creator.none.fl_str_mv Alesso, Magdalena
Fernandez, Liliana Patricia
author Alesso, Magdalena
author_facet Alesso, Magdalena
Fernandez, Liliana Patricia
author_role author
author2 Fernandez, Liliana Patricia
author2_role author
dc.subject.none.fl_str_mv Bovine Serum Albumin
Caffeine Determination
Energy Supplements
Flow Injection Analysis
Molecular Fluorescence
topic Bovine Serum Albumin
Caffeine Determination
Energy Supplements
Flow Injection Analysis
Molecular Fluorescence
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A new methodology for caffeine determination was developed based on the quenching effect on fluorescent emission of the molecule of Bovine Serum Albumin at λem = 338 nm (λex = 280). A flow injection disposition was designed improving significantly the sampling rate to 60 samples/h using potassium dihydrophosphate 5 · 10-3 mol L-1 buffer (pH 6.8) as carrier and flow rate of 1.5 mL min-1. The experimental and instrumental conditions that influence on analytical quality parameters were systematically investigated, as consider: buffer nature and concentration, fluorophore nature and concentration, and carrier flow rate. The proposal is simple, fast, inexpensive and precise, with a linear range from 6.68 · 10-6 to 4.0 · 10-3 mol L-1 and SD of 0.0668, under optimized conditions. Methodology sensibility and selectivity allowed a variety of sample analyses. It was successfully applied to caffeine quantification in energy drinks, dietary supplements and sliming infusion samples without previous treatment.
Fil: Alesso, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
description A new methodology for caffeine determination was developed based on the quenching effect on fluorescent emission of the molecule of Bovine Serum Albumin at λem = 338 nm (λex = 280). A flow injection disposition was designed improving significantly the sampling rate to 60 samples/h using potassium dihydrophosphate 5 · 10-3 mol L-1 buffer (pH 6.8) as carrier and flow rate of 1.5 mL min-1. The experimental and instrumental conditions that influence on analytical quality parameters were systematically investigated, as consider: buffer nature and concentration, fluorophore nature and concentration, and carrier flow rate. The proposal is simple, fast, inexpensive and precise, with a linear range from 6.68 · 10-6 to 4.0 · 10-3 mol L-1 and SD of 0.0668, under optimized conditions. Methodology sensibility and selectivity allowed a variety of sample analyses. It was successfully applied to caffeine quantification in energy drinks, dietary supplements and sliming infusion samples without previous treatment.
publishDate 2016
dc.date.none.fl_str_mv 2016-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/60470
Alesso, Magdalena; Fernandez, Liliana Patricia; Caffeine determination by flow injection analysis employing Bovine Serum Albumin as a fluorophore; Elsevier Science; Microchemical Journal; 127; 7-2016; 165-169
0026-265X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/60470
identifier_str_mv Alesso, Magdalena; Fernandez, Liliana Patricia; Caffeine determination by flow injection analysis employing Bovine Serum Albumin as a fluorophore; Elsevier Science; Microchemical Journal; 127; 7-2016; 165-169
0026-265X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2016.03.004
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0026265X16300017
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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