Caffeine quantification in dietary supplements by fluorescence using bovine serum albumin

Autores
Alesso, Magdalena; Santarossa, Debora Gimena; Kaplan, Marcos Manuel; Talio, María Carolina; Acosta, Maria Gimena; Fernandez, Liliana Patricia
Año de publicación
2014
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Caffeine (1,3,7-trimethylxanthine; CF), is a substance found naturally in the leaves, beans and fruits of a variety of plants. Although it is regularly consumed by a large percentage of adults, in Argentina recently the young have been added to this group. Attending to the health risks, many countries have started action to establish the regulatory boundaries around CF. A new method for the determination of caffeine based on the quenching of Bovine serum albumin (BSA) fluorescence. When the alkaloid CF is presents (quencher), the protein?s fluorescence emission is diminished. The diminution of the signal is proportional at caffeine concentration. The effect of experimental parameters, including the nature of the buffer and pH, and the nature of the surfactant and concentration were investigated by means univariation assays. Under optimum experimental conditions, a detection and quantification limits of 1.9 and 6.4 mg l−1 were obtained, respectively. A linear range was achieved varying from concentrations of 6.4 to 94 mg l−1 (r2 = 0.997). Satisfactory recovery values were obtained using the method of standard addition, confirming the feasibility of this method for caffeine determination in energising dietary supplements and energy drinks. In order to obtain the known advantages of on-line procedure, a flow injection manifold will be sketched and quality analytical parameter will be evaluated.
Fil: Alesso, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Santarossa, Debora Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Kaplan, Marcos Manuel. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina
Fil: Talio, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Acosta, Maria Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
3ra Reunión Internacional de Ciencias Farmacéuticas
Córdoba
Argentina
Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica
Materia
CAFFEINE MONITORING
BOVINE SERUM ALBUMIN
FLUORESCENT QUENCHING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/242856
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/242856
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Caffeine quantification in dietary supplements by fluorescence using bovine serum albuminAlesso, MagdalenaSantarossa, Debora GimenaKaplan, Marcos ManuelTalio, María CarolinaAcosta, Maria GimenaFernandez, Liliana PatriciaCAFFEINE MONITORINGBOVINE SERUM ALBUMINFLUORESCENT QUENCHINGhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Caffeine (1,3,7-trimethylxanthine; CF), is a substance found naturally in the leaves, beans and fruits of a variety of plants. Although it is regularly consumed by a large percentage of adults, in Argentina recently the young have been added to this group. Attending to the health risks, many countries have started action to establish the regulatory boundaries around CF. A new method for the determination of caffeine based on the quenching of Bovine serum albumin (BSA) fluorescence. When the alkaloid CF is presents (quencher), the protein?s fluorescence emission is diminished. The diminution of the signal is proportional at caffeine concentration. The effect of experimental parameters, including the nature of the buffer and pH, and the nature of the surfactant and concentration were investigated by means univariation assays. Under optimum experimental conditions, a detection and quantification limits of 1.9 and 6.4 mg l−1 were obtained, respectively. A linear range was achieved varying from concentrations of 6.4 to 94 mg l−1 (r2 = 0.997). Satisfactory recovery values were obtained using the method of standard addition, confirming the feasibility of this method for caffeine determination in energising dietary supplements and energy drinks. In order to obtain the known advantages of on-line procedure, a flow injection manifold will be sketched and quality analytical parameter will be evaluated.Fil: Alesso, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Santarossa, Debora Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Kaplan, Marcos Manuel. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; ArgentinaFil: Talio, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Acosta, Maria Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina3ra Reunión Internacional de Ciencias FarmacéuticasCórdobaArgentinaUniversidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología FarmacéuticaUniversidad Nacional de Córdoba2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/242856Caffeine quantification in dietary supplements by fluorescence using bovine serum albumin; 3ra Reunión Internacional de Ciencias Farmacéuticas; Córdoba; Argentina; 2014; 52-520975-8232CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.ijpsr.com/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:56Zoai:ri.conicet.gov.ar:11336/242856instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:56.682CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Caffeine quantification in dietary supplements by fluorescence using bovine serum albumin
title Caffeine quantification in dietary supplements by fluorescence using bovine serum albumin
spellingShingle Caffeine quantification in dietary supplements by fluorescence using bovine serum albumin
Alesso, Magdalena
CAFFEINE MONITORING
BOVINE SERUM ALBUMIN
FLUORESCENT QUENCHING
title_short Caffeine quantification in dietary supplements by fluorescence using bovine serum albumin
title_full Caffeine quantification in dietary supplements by fluorescence using bovine serum albumin
title_fullStr Caffeine quantification in dietary supplements by fluorescence using bovine serum albumin
title_full_unstemmed Caffeine quantification in dietary supplements by fluorescence using bovine serum albumin
title_sort Caffeine quantification in dietary supplements by fluorescence using bovine serum albumin
dc.creator.none.fl_str_mv Alesso, Magdalena
Santarossa, Debora Gimena
Kaplan, Marcos Manuel
Talio, María Carolina
Acosta, Maria Gimena
Fernandez, Liliana Patricia
author Alesso, Magdalena
author_facet Alesso, Magdalena
Santarossa, Debora Gimena
Kaplan, Marcos Manuel
Talio, María Carolina
Acosta, Maria Gimena
Fernandez, Liliana Patricia
author_role author
author2 Santarossa, Debora Gimena
Kaplan, Marcos Manuel
Talio, María Carolina
Acosta, Maria Gimena
Fernandez, Liliana Patricia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CAFFEINE MONITORING
BOVINE SERUM ALBUMIN
FLUORESCENT QUENCHING
topic CAFFEINE MONITORING
BOVINE SERUM ALBUMIN
FLUORESCENT QUENCHING
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Caffeine (1,3,7-trimethylxanthine; CF), is a substance found naturally in the leaves, beans and fruits of a variety of plants. Although it is regularly consumed by a large percentage of adults, in Argentina recently the young have been added to this group. Attending to the health risks, many countries have started action to establish the regulatory boundaries around CF. A new method for the determination of caffeine based on the quenching of Bovine serum albumin (BSA) fluorescence. When the alkaloid CF is presents (quencher), the protein?s fluorescence emission is diminished. The diminution of the signal is proportional at caffeine concentration. The effect of experimental parameters, including the nature of the buffer and pH, and the nature of the surfactant and concentration were investigated by means univariation assays. Under optimum experimental conditions, a detection and quantification limits of 1.9 and 6.4 mg l−1 were obtained, respectively. A linear range was achieved varying from concentrations of 6.4 to 94 mg l−1 (r2 = 0.997). Satisfactory recovery values were obtained using the method of standard addition, confirming the feasibility of this method for caffeine determination in energising dietary supplements and energy drinks. In order to obtain the known advantages of on-line procedure, a flow injection manifold will be sketched and quality analytical parameter will be evaluated.
Fil: Alesso, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Santarossa, Debora Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Kaplan, Marcos Manuel. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina
Fil: Talio, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Acosta, Maria Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
3ra Reunión Internacional de Ciencias Farmacéuticas
Córdoba
Argentina
Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica
description Caffeine (1,3,7-trimethylxanthine; CF), is a substance found naturally in the leaves, beans and fruits of a variety of plants. Although it is regularly consumed by a large percentage of adults, in Argentina recently the young have been added to this group. Attending to the health risks, many countries have started action to establish the regulatory boundaries around CF. A new method for the determination of caffeine based on the quenching of Bovine serum albumin (BSA) fluorescence. When the alkaloid CF is presents (quencher), the protein?s fluorescence emission is diminished. The diminution of the signal is proportional at caffeine concentration. The effect of experimental parameters, including the nature of the buffer and pH, and the nature of the surfactant and concentration were investigated by means univariation assays. Under optimum experimental conditions, a detection and quantification limits of 1.9 and 6.4 mg l−1 were obtained, respectively. A linear range was achieved varying from concentrations of 6.4 to 94 mg l−1 (r2 = 0.997). Satisfactory recovery values were obtained using the method of standard addition, confirming the feasibility of this method for caffeine determination in energising dietary supplements and energy drinks. In order to obtain the known advantages of on-line procedure, a flow injection manifold will be sketched and quality analytical parameter will be evaluated.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/242856
Caffeine quantification in dietary supplements by fluorescence using bovine serum albumin; 3ra Reunión Internacional de Ciencias Farmacéuticas; Córdoba; Argentina; 2014; 52-52
0975-8232
CONICET Digital
CONICET
url http://hdl.handle.net/11336/242856
identifier_str_mv Caffeine quantification in dietary supplements by fluorescence using bovine serum albumin; 3ra Reunión Internacional de Ciencias Farmacéuticas; Córdoba; Argentina; 2014; 52-52
0975-8232
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.ijpsr.com/
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application/pdf
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Universidad Nacional de Córdoba
publisher.none.fl_str_mv Universidad Nacional de Córdoba
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
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repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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