The Fusion Protein Signal-Peptide-Coding Region of Canine Distemper Virus: a Useful Tool for Phylogenetic Reconstruction and Lineage Identification

Autores
Sarute, Nicolás; Gallo Calderon, Marina Beatriz; Perez, Rubén; la Torre, Jose Leonardo; Hernández, Martín; Francia, Lourdes; Panzera, Yanina
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Canine distemper virus (CDV) (Paramyxoviridae, Morbillivirus) is the etiologic agent of a multisystemic infectious disease affecting all the terrestrial carnivore families, with high incidence and mortality in domestic dogs. The hemagglutinin (H) gene has been widely employed to characterize field strains, permitting the identification of the nine CDV lineages worldwide. Recently, it has been established that the fusion protein signal peptide coding region (Fsp) is extremely variable, suggesting its usefulness for characterization studies. However, the divergence among global strains and its phylogenetic resolution has not been evaluated yet. Herein, we constructed two datasets with the Fsp coding region and H gene sequences of the same strains belonging to eight CDV lineages, and used them to compare both genomic regions through phylogenetic and likelihood mapping analyses. The phylogenetic analysis revealed that both datasets clustered the strains in eight CDVs lineages. The amino acid divergence inter-lineages showed values for Fsp peptide four-fold higher than the H protein. The likelihood mapping study revealed that both datasets displayed strong phylogenetic signals in the region of well-resolved topology. These features indicated that the Fsp coding region is useful for evolutionary analysis as it allows the straightforward identification of CDV lineages.
Fil: Nicolas Sarute. UNIVERSIDAD DE LA REPUBLICA.
Fil: Gallo Calderon, Marina. CONSEJO NAC.DE INVEST.CIENTIF.Y TECNICAS. OFICINA DE COORDINACION ADMINISTRATIVA PQUE. CENTENARIO. INST.DE CS. Y TECNOLOGIA "DR. CESAR MILSTEIN".
Fil: Ruben Perez. UNIVERSIDAD DE LA REPUBLICA.
Fil: la Torre, Jose. CONSEJO NAC.DE INVEST.CIENTIF.Y TECNICAS. OFICINA DE COORDINACION ADMINISTRATIVA PQUE. CENTENARIO. INST.DE CS. Y TECNOLOGIA "DR. CESAR MILSTEIN".
Fil: Martín Hernández. UNIVERSIDAD DE LA REPUBLICA.
Fil: Lourdes Francia. UNIVERSIDAD DE LA REPUBLICA.
Fil: Yanina Panzera. UNIVERSIDAD DE LA REPUBLICA.
Materia
DISTEMPER CANINO
GENETIC LINEAGES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/497
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/497
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The Fusion Protein Signal-Peptide-Coding Region of Canine Distemper Virus: a Useful Tool for Phylogenetic Reconstruction and Lineage IdentificationSarute, NicolásGallo Calderon, Marina BeatrizPerez, Rubénla Torre, Jose LeonardoHernández, MartínFrancia, LourdesPanzera, YaninaDISTEMPER CANINOGENETIC LINEAGEShttps://purl.org/becyt/ford/4Canine distemper virus (CDV) (Paramyxoviridae, Morbillivirus) is the etiologic agent of a multisystemic infectious disease affecting all the terrestrial carnivore families, with high incidence and mortality in domestic dogs. The hemagglutinin (H) gene has been widely employed to characterize field strains, permitting the identification of the nine CDV lineages worldwide. Recently, it has been established that the fusion protein signal peptide coding region (Fsp) is extremely variable, suggesting its usefulness for characterization studies. However, the divergence among global strains and its phylogenetic resolution has not been evaluated yet. Herein, we constructed two datasets with the Fsp coding region and H gene sequences of the same strains belonging to eight CDV lineages, and used them to compare both genomic regions through phylogenetic and likelihood mapping analyses. The phylogenetic analysis revealed that both datasets clustered the strains in eight CDVs lineages. The amino acid divergence inter-lineages showed values for Fsp peptide four-fold higher than the H protein. The likelihood mapping study revealed that both datasets displayed strong phylogenetic signals in the region of well-resolved topology. These features indicated that the Fsp coding region is useful for evolutionary analysis as it allows the straightforward identification of CDV lineages.Fil: Nicolas Sarute. UNIVERSIDAD DE LA REPUBLICA.Fil: Gallo Calderon, Marina. CONSEJO NAC.DE INVEST.CIENTIF.Y TECNICAS. OFICINA DE COORDINACION ADMINISTRATIVA PQUE. CENTENARIO. INST.DE CS. Y TECNOLOGIA "DR. CESAR MILSTEIN".Fil: Ruben Perez. UNIVERSIDAD DE LA REPUBLICA.Fil: la Torre, Jose. CONSEJO NAC.DE INVEST.CIENTIF.Y TECNICAS. OFICINA DE COORDINACION ADMINISTRATIVA PQUE. CENTENARIO. INST.DE CS. Y TECNOLOGIA "DR. CESAR MILSTEIN".Fil: Martín Hernández. UNIVERSIDAD DE LA REPUBLICA.Fil: Lourdes Francia. UNIVERSIDAD DE LA REPUBLICA.Fil: Yanina Panzera. UNIVERSIDAD DE LA REPUBLICA.Public Library Science2013-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/497Nicolas Sarute; Gallo Calderon, Marina; Ruben Perez; la Torre, Jose; Martín Hernández; Lourdes Francia; Yanina Panzera; The Fusion Protein Signal-Peptide-Coding Region of Canine Distemper Virus: a Useful Tool for Phylogenetic Reconstruction and Lineage Identification; Public Library Science; Plos One; 8; 2013-5; 1-6;1932-6203enginfo:eu-repo/semantics/altIdentifier/url/http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0063595info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:40:42Zoai:ri.conicet.gov.ar:11336/497instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:40:43.092CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The Fusion Protein Signal-Peptide-Coding Region of Canine Distemper Virus: a Useful Tool for Phylogenetic Reconstruction and Lineage Identification
title The Fusion Protein Signal-Peptide-Coding Region of Canine Distemper Virus: a Useful Tool for Phylogenetic Reconstruction and Lineage Identification
spellingShingle The Fusion Protein Signal-Peptide-Coding Region of Canine Distemper Virus: a Useful Tool for Phylogenetic Reconstruction and Lineage Identification
Sarute, Nicolás
DISTEMPER CANINO
GENETIC LINEAGES
title_short The Fusion Protein Signal-Peptide-Coding Region of Canine Distemper Virus: a Useful Tool for Phylogenetic Reconstruction and Lineage Identification
title_full The Fusion Protein Signal-Peptide-Coding Region of Canine Distemper Virus: a Useful Tool for Phylogenetic Reconstruction and Lineage Identification
title_fullStr The Fusion Protein Signal-Peptide-Coding Region of Canine Distemper Virus: a Useful Tool for Phylogenetic Reconstruction and Lineage Identification
title_full_unstemmed The Fusion Protein Signal-Peptide-Coding Region of Canine Distemper Virus: a Useful Tool for Phylogenetic Reconstruction and Lineage Identification
title_sort The Fusion Protein Signal-Peptide-Coding Region of Canine Distemper Virus: a Useful Tool for Phylogenetic Reconstruction and Lineage Identification
dc.creator.none.fl_str_mv Sarute, Nicolás
Gallo Calderon, Marina Beatriz
Perez, Rubén
la Torre, Jose Leonardo
Hernández, Martín
Francia, Lourdes
Panzera, Yanina
author Sarute, Nicolás
author_facet Sarute, Nicolás
Gallo Calderon, Marina Beatriz
Perez, Rubén
la Torre, Jose Leonardo
Hernández, Martín
Francia, Lourdes
Panzera, Yanina
author_role author
author2 Gallo Calderon, Marina Beatriz
Perez, Rubén
la Torre, Jose Leonardo
Hernández, Martín
Francia, Lourdes
Panzera, Yanina
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv DISTEMPER CANINO
GENETIC LINEAGES
topic DISTEMPER CANINO
GENETIC LINEAGES
purl_subject.fl_str_mv https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Canine distemper virus (CDV) (Paramyxoviridae, Morbillivirus) is the etiologic agent of a multisystemic infectious disease affecting all the terrestrial carnivore families, with high incidence and mortality in domestic dogs. The hemagglutinin (H) gene has been widely employed to characterize field strains, permitting the identification of the nine CDV lineages worldwide. Recently, it has been established that the fusion protein signal peptide coding region (Fsp) is extremely variable, suggesting its usefulness for characterization studies. However, the divergence among global strains and its phylogenetic resolution has not been evaluated yet. Herein, we constructed two datasets with the Fsp coding region and H gene sequences of the same strains belonging to eight CDV lineages, and used them to compare both genomic regions through phylogenetic and likelihood mapping analyses. The phylogenetic analysis revealed that both datasets clustered the strains in eight CDVs lineages. The amino acid divergence inter-lineages showed values for Fsp peptide four-fold higher than the H protein. The likelihood mapping study revealed that both datasets displayed strong phylogenetic signals in the region of well-resolved topology. These features indicated that the Fsp coding region is useful for evolutionary analysis as it allows the straightforward identification of CDV lineages.
Fil: Nicolas Sarute. UNIVERSIDAD DE LA REPUBLICA.
Fil: Gallo Calderon, Marina. CONSEJO NAC.DE INVEST.CIENTIF.Y TECNICAS. OFICINA DE COORDINACION ADMINISTRATIVA PQUE. CENTENARIO. INST.DE CS. Y TECNOLOGIA "DR. CESAR MILSTEIN".
Fil: Ruben Perez. UNIVERSIDAD DE LA REPUBLICA.
Fil: la Torre, Jose. CONSEJO NAC.DE INVEST.CIENTIF.Y TECNICAS. OFICINA DE COORDINACION ADMINISTRATIVA PQUE. CENTENARIO. INST.DE CS. Y TECNOLOGIA "DR. CESAR MILSTEIN".
Fil: Martín Hernández. UNIVERSIDAD DE LA REPUBLICA.
Fil: Lourdes Francia. UNIVERSIDAD DE LA REPUBLICA.
Fil: Yanina Panzera. UNIVERSIDAD DE LA REPUBLICA.
description Canine distemper virus (CDV) (Paramyxoviridae, Morbillivirus) is the etiologic agent of a multisystemic infectious disease affecting all the terrestrial carnivore families, with high incidence and mortality in domestic dogs. The hemagglutinin (H) gene has been widely employed to characterize field strains, permitting the identification of the nine CDV lineages worldwide. Recently, it has been established that the fusion protein signal peptide coding region (Fsp) is extremely variable, suggesting its usefulness for characterization studies. However, the divergence among global strains and its phylogenetic resolution has not been evaluated yet. Herein, we constructed two datasets with the Fsp coding region and H gene sequences of the same strains belonging to eight CDV lineages, and used them to compare both genomic regions through phylogenetic and likelihood mapping analyses. The phylogenetic analysis revealed that both datasets clustered the strains in eight CDVs lineages. The amino acid divergence inter-lineages showed values for Fsp peptide four-fold higher than the H protein. The likelihood mapping study revealed that both datasets displayed strong phylogenetic signals in the region of well-resolved topology. These features indicated that the Fsp coding region is useful for evolutionary analysis as it allows the straightforward identification of CDV lineages.
publishDate 2013
dc.date.none.fl_str_mv 2013-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/497
Nicolas Sarute; Gallo Calderon, Marina; Ruben Perez; la Torre, Jose; Martín Hernández; Lourdes Francia; Yanina Panzera; The Fusion Protein Signal-Peptide-Coding Region of Canine Distemper Virus: a Useful Tool for Phylogenetic Reconstruction and Lineage Identification; Public Library Science; Plos One; 8; 2013-5; 1-6;
1932-6203
url http://hdl.handle.net/11336/497
identifier_str_mv Nicolas Sarute; Gallo Calderon, Marina; Ruben Perez; la Torre, Jose; Martín Hernández; Lourdes Francia; Yanina Panzera; The Fusion Protein Signal-Peptide-Coding Region of Canine Distemper Virus: a Useful Tool for Phylogenetic Reconstruction and Lineage Identification; Public Library Science; Plos One; 8; 2013-5; 1-6;
1932-6203
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0063595
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library Science
publisher.none.fl_str_mv Public Library Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082899895386112
score 13.22299

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