The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris

Autores
Tizzano, Marco Antonio; Sguazza, Guillermo Hernán; Picotto, Leandro Daniel; Echeverria, Maria Gabriela; Pecoraro, Marcelo Ricardo
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Canine distemper virus (CDV) is a pathogen which affects members of the Canidae family, causing an acute, often fatal, systemic disease. CDV is an RNA virus of the family Paramyxoviridae that contains two envelope glycoproteins: F and HA. In this study, we focused on the envelope glycoprotein F as the main target for neutralizing antibodies produced after infection or vaccination. The complete coding region of the protein (60 kDa) was expressed in the methylotrophic yeast Pichia pastoris, obtained in a recombinant form and secreted to the culture medium. Later, to analyze its immunogenicity, the protein was combined with an oily adjuvant and used to inoculate mice. The results provide evidence supporting a potential application of this recombinant protein as a subunit vaccine.
Fil: Tizzano, Marco Antonio. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Sguazza, Guillermo Hernán. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Picotto, Leandro Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Echeverria, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Pecoraro, Marcelo Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Materia
CANINE DISTEMPER VIRUS
GLYCOPROTEIN F
PICHIA PASTORIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/136593
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastorisTizzano, Marco AntonioSguazza, Guillermo HernánPicotto, Leandro DanielEcheverria, Maria GabrielaPecoraro, Marcelo RicardoCANINE DISTEMPER VIRUSGLYCOPROTEIN FPICHIA PASTORIShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Canine distemper virus (CDV) is a pathogen which affects members of the Canidae family, causing an acute, often fatal, systemic disease. CDV is an RNA virus of the family Paramyxoviridae that contains two envelope glycoproteins: F and HA. In this study, we focused on the envelope glycoprotein F as the main target for neutralizing antibodies produced after infection or vaccination. The complete coding region of the protein (60 kDa) was expressed in the methylotrophic yeast Pichia pastoris, obtained in a recombinant form and secreted to the culture medium. Later, to analyze its immunogenicity, the protein was combined with an oily adjuvant and used to inoculate mice. The results provide evidence supporting a potential application of this recombinant protein as a subunit vaccine.Fil: Tizzano, Marco Antonio. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Sguazza, Guillermo Hernán. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Picotto, Leandro Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Echeverria, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Pecoraro, Marcelo Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaAcademic Press Ltd - Elsevier Science Ltd2020-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/136593Tizzano, Marco Antonio; Sguazza, Guillermo Hernán; Picotto, Leandro Daniel; Echeverria, Maria Gabriela; Pecoraro, Marcelo Ricardo; The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris; Academic Press Ltd - Elsevier Science Ltd; Microbial Pathogenesis; 142; 5-2020; 1-17; 1040940882-40101096-1208CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0882401019321278info:eu-repo/semantics/altIdentifier/doi/10.1016/j.micpath.2020.104094info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:03:04Zoai:ri.conicet.gov.ar:11336/136593instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:03:05.314CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris
title The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris
spellingShingle The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris
Tizzano, Marco Antonio
CANINE DISTEMPER VIRUS
GLYCOPROTEIN F
PICHIA PASTORIS
title_short The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris
title_full The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris
title_fullStr The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris
title_full_unstemmed The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris
title_sort The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris
dc.creator.none.fl_str_mv Tizzano, Marco Antonio
Sguazza, Guillermo Hernán
Picotto, Leandro Daniel
Echeverria, Maria Gabriela
Pecoraro, Marcelo Ricardo
author Tizzano, Marco Antonio
author_facet Tizzano, Marco Antonio
Sguazza, Guillermo Hernán
Picotto, Leandro Daniel
Echeverria, Maria Gabriela
Pecoraro, Marcelo Ricardo
author_role author
author2 Sguazza, Guillermo Hernán
Picotto, Leandro Daniel
Echeverria, Maria Gabriela
Pecoraro, Marcelo Ricardo
author2_role author
author
author
author
dc.subject.none.fl_str_mv CANINE DISTEMPER VIRUS
GLYCOPROTEIN F
PICHIA PASTORIS
topic CANINE DISTEMPER VIRUS
GLYCOPROTEIN F
PICHIA PASTORIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Canine distemper virus (CDV) is a pathogen which affects members of the Canidae family, causing an acute, often fatal, systemic disease. CDV is an RNA virus of the family Paramyxoviridae that contains two envelope glycoproteins: F and HA. In this study, we focused on the envelope glycoprotein F as the main target for neutralizing antibodies produced after infection or vaccination. The complete coding region of the protein (60 kDa) was expressed in the methylotrophic yeast Pichia pastoris, obtained in a recombinant form and secreted to the culture medium. Later, to analyze its immunogenicity, the protein was combined with an oily adjuvant and used to inoculate mice. The results provide evidence supporting a potential application of this recombinant protein as a subunit vaccine.
Fil: Tizzano, Marco Antonio. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Sguazza, Guillermo Hernán. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Picotto, Leandro Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Echeverria, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Pecoraro, Marcelo Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
description Canine distemper virus (CDV) is a pathogen which affects members of the Canidae family, causing an acute, often fatal, systemic disease. CDV is an RNA virus of the family Paramyxoviridae that contains two envelope glycoproteins: F and HA. In this study, we focused on the envelope glycoprotein F as the main target for neutralizing antibodies produced after infection or vaccination. The complete coding region of the protein (60 kDa) was expressed in the methylotrophic yeast Pichia pastoris, obtained in a recombinant form and secreted to the culture medium. Later, to analyze its immunogenicity, the protein was combined with an oily adjuvant and used to inoculate mice. The results provide evidence supporting a potential application of this recombinant protein as a subunit vaccine.
publishDate 2020
dc.date.none.fl_str_mv 2020-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/136593
Tizzano, Marco Antonio; Sguazza, Guillermo Hernán; Picotto, Leandro Daniel; Echeverria, Maria Gabriela; Pecoraro, Marcelo Ricardo; The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris; Academic Press Ltd - Elsevier Science Ltd; Microbial Pathogenesis; 142; 5-2020; 1-17; 104094
0882-4010
1096-1208
CONICET Digital
CONICET
url http://hdl.handle.net/11336/136593
identifier_str_mv Tizzano, Marco Antonio; Sguazza, Guillermo Hernán; Picotto, Leandro Daniel; Echeverria, Maria Gabriela; Pecoraro, Marcelo Ricardo; The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris; Academic Press Ltd - Elsevier Science Ltd; Microbial Pathogenesis; 142; 5-2020; 1-17; 104094
0882-4010
1096-1208
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0882401019321278
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.micpath.2020.104094
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Ltd - Elsevier Science Ltd
publisher.none.fl_str_mv Academic Press Ltd - Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269781405728768
score 13.13397

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