Multiplexed imaging of intracellular protein networks

Autores
Grecco, Hernan Edgardo; Imtiaz, S.; Zamir, E.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cellular functions emerge from the collective action of a large number of different proteins. Understanding how these protein networks operate requires monitoring their components in intact cells. Due to intercellular and intracellular molecular variability, it is important to monitor simultaneously multiple components at high spatiotemporal resolution. However, inherent trade-offs narrow the boundaries of achievable multiplexed imaging. Pushing these boundaries is essential for a better understanding of cellular processes. Here the motivations, challenges and approaches for multiplexed imaging of intracellular protein networks are discussed.
Fil: Grecco, Hernan Edgardo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina
Fil: Imtiaz, S.. Institut Max Planck fur Molekulare Physiologie; Alemania
Fil: Zamir, E.. Institut Max Planck fur Molekulare Physiologie; Alemania
Materia
Multicolor Imaging
High-Throughput Microscopy
Multispectral Imaging
Spectral Unmixing
Immunofluorescence
Fluorescent Proteins
Cyclic Immunofluorescence
Live Cell Imaging
Cell-To-Cell Variability
Spatial Organization
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/47647
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/47647
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Multiplexed imaging of intracellular protein networksGrecco, Hernan EdgardoImtiaz, S.Zamir, E.Multicolor ImagingHigh-Throughput MicroscopyMultispectral ImagingSpectral UnmixingImmunofluorescenceFluorescent ProteinsCyclic ImmunofluorescenceLive Cell ImagingCell-To-Cell VariabilitySpatial Organizationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Cellular functions emerge from the collective action of a large number of different proteins. Understanding how these protein networks operate requires monitoring their components in intact cells. Due to intercellular and intracellular molecular variability, it is important to monitor simultaneously multiple components at high spatiotemporal resolution. However, inherent trade-offs narrow the boundaries of achievable multiplexed imaging. Pushing these boundaries is essential for a better understanding of cellular processes. Here the motivations, challenges and approaches for multiplexed imaging of intracellular protein networks are discussed.Fil: Grecco, Hernan Edgardo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; ArgentinaFil: Imtiaz, S.. Institut Max Planck fur Molekulare Physiologie; AlemaniaFil: Zamir, E.. Institut Max Planck fur Molekulare Physiologie; AlemaniaWiley-liss, Div John Wiley & Sons Inc2016-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/47647Grecco, Hernan Edgardo; Imtiaz, S.; Zamir, E.; Multiplexed imaging of intracellular protein networks; Wiley-liss, Div John Wiley & Sons Inc; Cytometry Part A; 5-20161552-4922CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/cyto.a.22876/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1002/cyto.a.22876info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:50:42Zoai:ri.conicet.gov.ar:11336/47647instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:50:42.467CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Multiplexed imaging of intracellular protein networks
title Multiplexed imaging of intracellular protein networks
spellingShingle Multiplexed imaging of intracellular protein networks
Grecco, Hernan Edgardo
Multicolor Imaging
High-Throughput Microscopy
Multispectral Imaging
Spectral Unmixing
Immunofluorescence
Fluorescent Proteins
Cyclic Immunofluorescence
Live Cell Imaging
Cell-To-Cell Variability
Spatial Organization
title_short Multiplexed imaging of intracellular protein networks
title_full Multiplexed imaging of intracellular protein networks
title_fullStr Multiplexed imaging of intracellular protein networks
title_full_unstemmed Multiplexed imaging of intracellular protein networks
title_sort Multiplexed imaging of intracellular protein networks
dc.creator.none.fl_str_mv Grecco, Hernan Edgardo
Imtiaz, S.
Zamir, E.
author Grecco, Hernan Edgardo
author_facet Grecco, Hernan Edgardo
Imtiaz, S.
Zamir, E.
author_role author
author2 Imtiaz, S.
Zamir, E.
author2_role author
author
dc.subject.none.fl_str_mv Multicolor Imaging
High-Throughput Microscopy
Multispectral Imaging
Spectral Unmixing
Immunofluorescence
Fluorescent Proteins
Cyclic Immunofluorescence
Live Cell Imaging
Cell-To-Cell Variability
Spatial Organization
topic Multicolor Imaging
High-Throughput Microscopy
Multispectral Imaging
Spectral Unmixing
Immunofluorescence
Fluorescent Proteins
Cyclic Immunofluorescence
Live Cell Imaging
Cell-To-Cell Variability
Spatial Organization
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Cellular functions emerge from the collective action of a large number of different proteins. Understanding how these protein networks operate requires monitoring their components in intact cells. Due to intercellular and intracellular molecular variability, it is important to monitor simultaneously multiple components at high spatiotemporal resolution. However, inherent trade-offs narrow the boundaries of achievable multiplexed imaging. Pushing these boundaries is essential for a better understanding of cellular processes. Here the motivations, challenges and approaches for multiplexed imaging of intracellular protein networks are discussed.
Fil: Grecco, Hernan Edgardo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina
Fil: Imtiaz, S.. Institut Max Planck fur Molekulare Physiologie; Alemania
Fil: Zamir, E.. Institut Max Planck fur Molekulare Physiologie; Alemania
description Cellular functions emerge from the collective action of a large number of different proteins. Understanding how these protein networks operate requires monitoring their components in intact cells. Due to intercellular and intracellular molecular variability, it is important to monitor simultaneously multiple components at high spatiotemporal resolution. However, inherent trade-offs narrow the boundaries of achievable multiplexed imaging. Pushing these boundaries is essential for a better understanding of cellular processes. Here the motivations, challenges and approaches for multiplexed imaging of intracellular protein networks are discussed.
publishDate 2016
dc.date.none.fl_str_mv 2016-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/47647
Grecco, Hernan Edgardo; Imtiaz, S.; Zamir, E.; Multiplexed imaging of intracellular protein networks; Wiley-liss, Div John Wiley & Sons Inc; Cytometry Part A; 5-2016
1552-4922
CONICET Digital
CONICET
url http://hdl.handle.net/11336/47647
identifier_str_mv Grecco, Hernan Edgardo; Imtiaz, S.; Zamir, E.; Multiplexed imaging of intracellular protein networks; Wiley-liss, Div John Wiley & Sons Inc; Cytometry Part A; 5-2016
1552-4922
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/cyto.a.22876/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1002/cyto.a.22876
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613562425671680
score 13.070432

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