Imaging amyloid precursor protein in vivo: An axonal transport assay

Autores
Falzone, Tomas Luis; Stokin, Gorazd B.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fusion of fluorescent probes to axonally transported proteins represents an established approach that enables live imaging of axonal transport. In this approach, in vivo examination of fluorescent particle dynamics provides information about the length, directionality, and the velocity by which axonally transported proteins travel along axons. Analysis of these parameters provides information about the distribution of axonal proteins and their dynamics in and between different subcellular compartments. Establishing the movement behavior of amyloid precursor protein within axons indicated that live imaging approaches offer the opportunity to significantly enhance our understanding of the biology as well as pathology of axonal transport. This chapter provides a fluorescence-based procedure for measuring axonal transport of APP in cultured newborn mouse hippocampal neurons. © 2012 Springer Science+Business Media, LLC.
Fil: Falzone, Tomas Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencias; Argentina
Fil: Stokin, Gorazd B.. University Medical Center; Eslovenia
Materia
Amyloid Precursor Protein
Axonal Transport
Fluorescent Probes
In Vivo Fluorescent Imaging
Primary Hippocampal Cell Culture
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/67300
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/67300
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Imaging amyloid precursor protein in vivo: An axonal transport assayFalzone, Tomas LuisStokin, Gorazd B.Amyloid Precursor ProteinAxonal TransportFluorescent ProbesIn Vivo Fluorescent ImagingPrimary Hippocampal Cell Culturehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Fusion of fluorescent probes to axonally transported proteins represents an established approach that enables live imaging of axonal transport. In this approach, in vivo examination of fluorescent particle dynamics provides information about the length, directionality, and the velocity by which axonally transported proteins travel along axons. Analysis of these parameters provides information about the distribution of axonal proteins and their dynamics in and between different subcellular compartments. Establishing the movement behavior of amyloid precursor protein within axons indicated that live imaging approaches offer the opportunity to significantly enhance our understanding of the biology as well as pathology of axonal transport. This chapter provides a fluorescence-based procedure for measuring axonal transport of APP in cultured newborn mouse hippocampal neurons. © 2012 Springer Science+Business Media, LLC.Fil: Falzone, Tomas Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencias; ArgentinaFil: Stokin, Gorazd B.. University Medical Center; EsloveniaHumana Press2012-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/67300Falzone, Tomas Luis; Stokin, Gorazd B.; Imaging amyloid precursor protein in vivo: An axonal transport assay; Humana Press; Methods in Molecular Biology; 846; 8-2012; 295-3031064-3745CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/978-1-61779-536-7_25info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/protocol/10.1007%2F978-1-61779-536-7_25info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:51:59Zoai:ri.conicet.gov.ar:11336/67300instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:51:59.599CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Imaging amyloid precursor protein in vivo: An axonal transport assay
title Imaging amyloid precursor protein in vivo: An axonal transport assay
spellingShingle Imaging amyloid precursor protein in vivo: An axonal transport assay
Falzone, Tomas Luis
Amyloid Precursor Protein
Axonal Transport
Fluorescent Probes
In Vivo Fluorescent Imaging
Primary Hippocampal Cell Culture
title_short Imaging amyloid precursor protein in vivo: An axonal transport assay
title_full Imaging amyloid precursor protein in vivo: An axonal transport assay
title_fullStr Imaging amyloid precursor protein in vivo: An axonal transport assay
title_full_unstemmed Imaging amyloid precursor protein in vivo: An axonal transport assay
title_sort Imaging amyloid precursor protein in vivo: An axonal transport assay
dc.creator.none.fl_str_mv Falzone, Tomas Luis
Stokin, Gorazd B.
author Falzone, Tomas Luis
author_facet Falzone, Tomas Luis
Stokin, Gorazd B.
author_role author
author2 Stokin, Gorazd B.
author2_role author
dc.subject.none.fl_str_mv Amyloid Precursor Protein
Axonal Transport
Fluorescent Probes
In Vivo Fluorescent Imaging
Primary Hippocampal Cell Culture
topic Amyloid Precursor Protein
Axonal Transport
Fluorescent Probes
In Vivo Fluorescent Imaging
Primary Hippocampal Cell Culture
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Fusion of fluorescent probes to axonally transported proteins represents an established approach that enables live imaging of axonal transport. In this approach, in vivo examination of fluorescent particle dynamics provides information about the length, directionality, and the velocity by which axonally transported proteins travel along axons. Analysis of these parameters provides information about the distribution of axonal proteins and their dynamics in and between different subcellular compartments. Establishing the movement behavior of amyloid precursor protein within axons indicated that live imaging approaches offer the opportunity to significantly enhance our understanding of the biology as well as pathology of axonal transport. This chapter provides a fluorescence-based procedure for measuring axonal transport of APP in cultured newborn mouse hippocampal neurons. © 2012 Springer Science+Business Media, LLC.
Fil: Falzone, Tomas Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencias; Argentina
Fil: Stokin, Gorazd B.. University Medical Center; Eslovenia
description Fusion of fluorescent probes to axonally transported proteins represents an established approach that enables live imaging of axonal transport. In this approach, in vivo examination of fluorescent particle dynamics provides information about the length, directionality, and the velocity by which axonally transported proteins travel along axons. Analysis of these parameters provides information about the distribution of axonal proteins and their dynamics in and between different subcellular compartments. Establishing the movement behavior of amyloid precursor protein within axons indicated that live imaging approaches offer the opportunity to significantly enhance our understanding of the biology as well as pathology of axonal transport. This chapter provides a fluorescence-based procedure for measuring axonal transport of APP in cultured newborn mouse hippocampal neurons. © 2012 Springer Science+Business Media, LLC.
publishDate 2012
dc.date.none.fl_str_mv 2012-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/67300
Falzone, Tomas Luis; Stokin, Gorazd B.; Imaging amyloid precursor protein in vivo: An axonal transport assay; Humana Press; Methods in Molecular Biology; 846; 8-2012; 295-303
1064-3745
CONICET Digital
CONICET
url http://hdl.handle.net/11336/67300
identifier_str_mv Falzone, Tomas Luis; Stokin, Gorazd B.; Imaging amyloid precursor protein in vivo: An axonal transport assay; Humana Press; Methods in Molecular Biology; 846; 8-2012; 295-303
1064-3745
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/978-1-61779-536-7_25
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/protocol/10.1007%2F978-1-61779-536-7_25
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Humana Press
publisher.none.fl_str_mv Humana Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269128683945984
score 13.13397

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