Clinical outcome and vertical transmission variability among canine Neospora caninum isolates in a pregnant mouse model of infection

Autores
Dellarupe, Andrea; Regidor Cerrillo, Javier; Jimenez Ruiz, Elena; Schares, Gereon; Unzaga, Juan Manuel; Venturini, María Cecilia; Ortega Mora, Luis M.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We compared the clinical outcome and vertical transmission of six canine Neospora caninum isolates using a pregnant BALB/c model. Four of the isolates were obtained from oocysts of naturally infected dogs (Nc-Ger2, Nc-Ger3, Nc-Ger6 and Nc-6Arg) and two were from diseased dogs with neurological signs (Nc-Bahia and Nc-Liv). The dams were inoculated with 2×106 tachyzoites of each isolate at day 7 of pregnancy. Morbidity, mortality and the antibody responses were evaluated in both the dams and the offspring, as was parasite transmission to the progeny. The mortality rates varied from 100% in Nc-Bahia and Nc-Liv-infected pups to 19% or less for those infected with the isolates from oocysts. The vertical transmission rates varied from 9 to 53% for N. caninum from oocysts, compared with 100% for the Nc-Liv and Nc-Bahia isolates. All dams showed specific IgG responses against tachyzoite and rNc-GRA7 antigens, confirming Neospora infection. The highest IgG levels were detected in mice inoculated with the Nc-Liv and Nc-Bahia isolates. These results demonstrate marked differences in virulence between the N. caninum isolates obtained from oocysts and neurologically affected dogs. This variability could help us to explain the differences in the outcome of the infection in definitive and intermediate hosts.
Fil: Dellarupe, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Complutense de Madrid; España. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Regidor Cerrillo, Javier. Universidad Complutense de Madrid; España
Fil: Jimenez Ruiz, Elena. Universidad Complutense de Madrid; España
Fil: Schares, Gereon. Friedrich Loeffler Institut; Alemania
Fil: Unzaga, Juan Manuel. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Venturini, María Cecilia. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ortega Mora, Luis M.. Universidad Complutense de Madrid; España
Materia
N. Caninum
In Vivo
Vertical Transmission
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/31409
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/31409
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Clinical outcome and vertical transmission variability among canine Neospora caninum isolates in a pregnant mouse model of infectionDellarupe, AndreaRegidor Cerrillo, JavierJimenez Ruiz, ElenaSchares, GereonUnzaga, Juan ManuelVenturini, María CeciliaOrtega Mora, Luis M.N. CaninumIn VivoVertical Transmissionhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4We compared the clinical outcome and vertical transmission of six canine Neospora caninum isolates using a pregnant BALB/c model. Four of the isolates were obtained from oocysts of naturally infected dogs (Nc-Ger2, Nc-Ger3, Nc-Ger6 and Nc-6Arg) and two were from diseased dogs with neurological signs (Nc-Bahia and Nc-Liv). The dams were inoculated with 2×106 tachyzoites of each isolate at day 7 of pregnancy. Morbidity, mortality and the antibody responses were evaluated in both the dams and the offspring, as was parasite transmission to the progeny. The mortality rates varied from 100% in Nc-Bahia and Nc-Liv-infected pups to 19% or less for those infected with the isolates from oocysts. The vertical transmission rates varied from 9 to 53% for N. caninum from oocysts, compared with 100% for the Nc-Liv and Nc-Bahia isolates. All dams showed specific IgG responses against tachyzoite and rNc-GRA7 antigens, confirming Neospora infection. The highest IgG levels were detected in mice inoculated with the Nc-Liv and Nc-Bahia isolates. These results demonstrate marked differences in virulence between the N. caninum isolates obtained from oocysts and neurologically affected dogs. This variability could help us to explain the differences in the outcome of the infection in definitive and intermediate hosts.Fil: Dellarupe, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Complutense de Madrid; España. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; ArgentinaFil: Regidor Cerrillo, Javier. Universidad Complutense de Madrid; EspañaFil: Jimenez Ruiz, Elena. Universidad Complutense de Madrid; EspañaFil: Schares, Gereon. Friedrich Loeffler Institut; AlemaniaFil: Unzaga, Juan Manuel. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; ArgentinaFil: Venturini, María Cecilia. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ortega Mora, Luis M.. Universidad Complutense de Madrid; EspañaCambridge University Press2013-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/31409Ortega Mora, Luis M.; Venturini, María Cecilia; Unzaga, Juan Manuel; Schares, Gereon; Jimenez Ruiz, Elena; Regidor Cerrillo, Javier; et al.; Clinical outcome and vertical transmission variability among canine Neospora caninum isolates in a pregnant mouse model of infection; Cambridge University Press; Parasitology; 141; 3; 10-2013; 356-3660031-1820CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1017/S0031182013001479info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/parasitology/article/clinical-outcome-and-vertical-transmission-variability-among-canine-neospora-caninum-isolates-in-a-pregnant-mouse-model-of-infection/952DEFE4FBD93098AD855BD3B408492Finfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:05:10Zoai:ri.conicet.gov.ar:11336/31409instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:05:10.332CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Clinical outcome and vertical transmission variability among canine Neospora caninum isolates in a pregnant mouse model of infection
title Clinical outcome and vertical transmission variability among canine Neospora caninum isolates in a pregnant mouse model of infection
spellingShingle Clinical outcome and vertical transmission variability among canine Neospora caninum isolates in a pregnant mouse model of infection
Dellarupe, Andrea
N. Caninum
In Vivo
Vertical Transmission
title_short Clinical outcome and vertical transmission variability among canine Neospora caninum isolates in a pregnant mouse model of infection
title_full Clinical outcome and vertical transmission variability among canine Neospora caninum isolates in a pregnant mouse model of infection
title_fullStr Clinical outcome and vertical transmission variability among canine Neospora caninum isolates in a pregnant mouse model of infection
title_full_unstemmed Clinical outcome and vertical transmission variability among canine Neospora caninum isolates in a pregnant mouse model of infection
title_sort Clinical outcome and vertical transmission variability among canine Neospora caninum isolates in a pregnant mouse model of infection
dc.creator.none.fl_str_mv Dellarupe, Andrea
Regidor Cerrillo, Javier
Jimenez Ruiz, Elena
Schares, Gereon
Unzaga, Juan Manuel
Venturini, María Cecilia
Ortega Mora, Luis M.
author Dellarupe, Andrea
author_facet Dellarupe, Andrea
Regidor Cerrillo, Javier
Jimenez Ruiz, Elena
Schares, Gereon
Unzaga, Juan Manuel
Venturini, María Cecilia
Ortega Mora, Luis M.
author_role author
author2 Regidor Cerrillo, Javier
Jimenez Ruiz, Elena
Schares, Gereon
Unzaga, Juan Manuel
Venturini, María Cecilia
Ortega Mora, Luis M.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv N. Caninum
In Vivo
Vertical Transmission
topic N. Caninum
In Vivo
Vertical Transmission
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv We compared the clinical outcome and vertical transmission of six canine Neospora caninum isolates using a pregnant BALB/c model. Four of the isolates were obtained from oocysts of naturally infected dogs (Nc-Ger2, Nc-Ger3, Nc-Ger6 and Nc-6Arg) and two were from diseased dogs with neurological signs (Nc-Bahia and Nc-Liv). The dams were inoculated with 2×106 tachyzoites of each isolate at day 7 of pregnancy. Morbidity, mortality and the antibody responses were evaluated in both the dams and the offspring, as was parasite transmission to the progeny. The mortality rates varied from 100% in Nc-Bahia and Nc-Liv-infected pups to 19% or less for those infected with the isolates from oocysts. The vertical transmission rates varied from 9 to 53% for N. caninum from oocysts, compared with 100% for the Nc-Liv and Nc-Bahia isolates. All dams showed specific IgG responses against tachyzoite and rNc-GRA7 antigens, confirming Neospora infection. The highest IgG levels were detected in mice inoculated with the Nc-Liv and Nc-Bahia isolates. These results demonstrate marked differences in virulence between the N. caninum isolates obtained from oocysts and neurologically affected dogs. This variability could help us to explain the differences in the outcome of the infection in definitive and intermediate hosts.
Fil: Dellarupe, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Complutense de Madrid; España. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Regidor Cerrillo, Javier. Universidad Complutense de Madrid; España
Fil: Jimenez Ruiz, Elena. Universidad Complutense de Madrid; España
Fil: Schares, Gereon. Friedrich Loeffler Institut; Alemania
Fil: Unzaga, Juan Manuel. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Venturini, María Cecilia. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ortega Mora, Luis M.. Universidad Complutense de Madrid; España
description We compared the clinical outcome and vertical transmission of six canine Neospora caninum isolates using a pregnant BALB/c model. Four of the isolates were obtained from oocysts of naturally infected dogs (Nc-Ger2, Nc-Ger3, Nc-Ger6 and Nc-6Arg) and two were from diseased dogs with neurological signs (Nc-Bahia and Nc-Liv). The dams were inoculated with 2×106 tachyzoites of each isolate at day 7 of pregnancy. Morbidity, mortality and the antibody responses were evaluated in both the dams and the offspring, as was parasite transmission to the progeny. The mortality rates varied from 100% in Nc-Bahia and Nc-Liv-infected pups to 19% or less for those infected with the isolates from oocysts. The vertical transmission rates varied from 9 to 53% for N. caninum from oocysts, compared with 100% for the Nc-Liv and Nc-Bahia isolates. All dams showed specific IgG responses against tachyzoite and rNc-GRA7 antigens, confirming Neospora infection. The highest IgG levels were detected in mice inoculated with the Nc-Liv and Nc-Bahia isolates. These results demonstrate marked differences in virulence between the N. caninum isolates obtained from oocysts and neurologically affected dogs. This variability could help us to explain the differences in the outcome of the infection in definitive and intermediate hosts.
publishDate 2013
dc.date.none.fl_str_mv 2013-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/31409
Ortega Mora, Luis M.; Venturini, María Cecilia; Unzaga, Juan Manuel; Schares, Gereon; Jimenez Ruiz, Elena; Regidor Cerrillo, Javier; et al.; Clinical outcome and vertical transmission variability among canine Neospora caninum isolates in a pregnant mouse model of infection; Cambridge University Press; Parasitology; 141; 3; 10-2013; 356-366
0031-1820
CONICET Digital
CONICET
url http://hdl.handle.net/11336/31409
identifier_str_mv Ortega Mora, Luis M.; Venturini, María Cecilia; Unzaga, Juan Manuel; Schares, Gereon; Jimenez Ruiz, Elena; Regidor Cerrillo, Javier; et al.; Clinical outcome and vertical transmission variability among canine Neospora caninum isolates in a pregnant mouse model of infection; Cambridge University Press; Parasitology; 141; 3; 10-2013; 356-366
0031-1820
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1017/S0031182013001479
info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/parasitology/article/clinical-outcome-and-vertical-transmission-variability-among-canine-neospora-caninum-isolates-in-a-pregnant-mouse-model-of-infection/952DEFE4FBD93098AD855BD3B408492F
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cambridge University Press
publisher.none.fl_str_mv Cambridge University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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