Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditis

Autores: Pardini, Lais Luján; Moré, Gastón Andrés; Rudzinski, Marcelo Nicolas; Gos, Maria Laura; Campero, Lucía María; Meyer, Alejandro; Bernstein, Mariana; Unzaga, Juan Manuel; Venturini, María Cecilia
Año de publicación: 2016
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: The aim of this study was to detect, isolate and genetically characterize Toxoplasma gondii from tissues obtained from free range chickens which were breed in farms from patients with toxoplasmic retinochoroiditis in Misiones, Argentina. Thirty three samples of head (refrigerated = 18 and frozen = 15) from free range chickens were processed. Refrigerated (n = 18) chicken central nervous systems (CNS) were bioassay in mice. DNA was obtained from all samples (n = 33) and PCR was performed using TOX5-TOX8 T. gondii specific primers. Positive PCR samples were characterized by nested-PCR and restriction fragment length polymorphism using the markers SAG2, BTUB, GRA6, SAG3, PK1, L358, C22-8, C29-2 and Apico. T. gondii DNA was amplified in 30.3% (10/33) of CNS samples. Isolates were obtained in 27.7% (5/18) of inoculated CNS samples (TgCk11-9Arg, TgCk13-5Arg, TgCk14-5Arg, TgCk14-6Arg and TgCk14-7Arg). Seven samples showed a restriction pattern to all markers and were identified as atypical with several alleles type III. Genotyping of T. gondii from samples of patients with retinochoroiditis in the same area could improve the understanding of the epidemiology of toxoplasmosis in the region.
Fil: Pardini, Lais Luján. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Moré, Gastón Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Rudzinski, Marcelo Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de uveítis y glaucoma; Argentina. Universidad Católica de las Misiones; Argentina
Fil: Gos, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Campero, Lucía María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Meyer, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Laboratorio de análisis clínicos Dr Meyer; Argentina
Fil: Bernstein, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Unzaga, Juan Manuel. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Venturini, María Cecilia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Materia: Free-Range Chickens
Genotyping
Isolates
Retichoroiditis
Toxoplasma Gondii
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/54303

Acceder

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network_name_str	CONICET Digital (CONICET)
spelling	Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditisPardini, Lais LujánMoré, Gastón AndrésRudzinski, Marcelo NicolasGos, Maria LauraCampero, Lucía MaríaMeyer, AlejandroBernstein, MarianaUnzaga, Juan ManuelVenturini, María CeciliaFree-Range ChickensGenotypingIsolatesRetichoroiditisToxoplasma Gondiihttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The aim of this study was to detect, isolate and genetically characterize Toxoplasma gondii from tissues obtained from free range chickens which were breed in farms from patients with toxoplasmic retinochoroiditis in Misiones, Argentina. Thirty three samples of head (refrigerated = 18 and frozen = 15) from free range chickens were processed. Refrigerated (n = 18) chicken central nervous systems (CNS) were bioassay in mice. DNA was obtained from all samples (n = 33) and PCR was performed using TOX5-TOX8 T. gondii specific primers. Positive PCR samples were characterized by nested-PCR and restriction fragment length polymorphism using the markers SAG2, BTUB, GRA6, SAG3, PK1, L358, C22-8, C29-2 and Apico. T. gondii DNA was amplified in 30.3% (10/33) of CNS samples. Isolates were obtained in 27.7% (5/18) of inoculated CNS samples (TgCk11-9Arg, TgCk13-5Arg, TgCk14-5Arg, TgCk14-6Arg and TgCk14-7Arg). Seven samples showed a restriction pattern to all markers and were identified as atypical with several alleles type III. Genotyping of T. gondii from samples of patients with retinochoroiditis in the same area could improve the understanding of the epidemiology of toxoplasmosis in the region.Fil: Pardini, Lais Luján. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; ArgentinaFil: Moré, Gastón Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; ArgentinaFil: Rudzinski, Marcelo Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de uveítis y glaucoma; Argentina. Universidad Católica de las Misiones; ArgentinaFil: Gos, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; ArgentinaFil: Campero, Lucía María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; ArgentinaFil: Meyer, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Laboratorio de análisis clínicos Dr Meyer; ArgentinaFil: Bernstein, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; ArgentinaFil: Unzaga, Juan Manuel. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; ArgentinaFil: Venturini, María Cecilia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; ArgentinaAcademic Press Inc Elsevier Science2016-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/54303Pardini, Lais Luján; Moré, Gastón Andrés; Rudzinski, Marcelo Nicolas; Gos, Maria Laura; Campero, Lucía María; et al.; Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditis; Academic Press Inc Elsevier Science; Experimental Parasitology; 166; 7-2016; 16-200014-4894CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.exppara.2016.03.006info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0014489416300327info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:42:41Zoai:ri.conicet.gov.ar:11336/54303instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:42:41.607CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditis
title	Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditis
spellingShingle	Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditis Pardini, Lais Luján Free-Range Chickens Genotyping Isolates Retichoroiditis Toxoplasma Gondii
title_short	Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditis
title_full	Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditis
title_fullStr	Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditis
title_full_unstemmed	Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditis
title_sort	Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditis
dc.creator.none.fl_str_mv	Pardini, Lais Luján Moré, Gastón Andrés Rudzinski, Marcelo Nicolas Gos, Maria Laura Campero, Lucía María Meyer, Alejandro Bernstein, Mariana Unzaga, Juan Manuel Venturini, María Cecilia
author	Pardini, Lais Luján
author_facet	Pardini, Lais Luján Moré, Gastón Andrés Rudzinski, Marcelo Nicolas Gos, Maria Laura Campero, Lucía María Meyer, Alejandro Bernstein, Mariana Unzaga, Juan Manuel Venturini, María Cecilia
author_role	author
author2	Moré, Gastón Andrés Rudzinski, Marcelo Nicolas Gos, Maria Laura Campero, Lucía María Meyer, Alejandro Bernstein, Mariana Unzaga, Juan Manuel Venturini, María Cecilia
author2_role	author author author author author author author author
dc.subject.none.fl_str_mv	Free-Range Chickens Genotyping Isolates Retichoroiditis Toxoplasma Gondii
topic	Free-Range Chickens Genotyping Isolates Retichoroiditis Toxoplasma Gondii
purl_subject.fl_str_mv	https://purl.org/becyt/ford/4.3 https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv	The aim of this study was to detect, isolate and genetically characterize Toxoplasma gondii from tissues obtained from free range chickens which were breed in farms from patients with toxoplasmic retinochoroiditis in Misiones, Argentina. Thirty three samples of head (refrigerated = 18 and frozen = 15) from free range chickens were processed. Refrigerated (n = 18) chicken central nervous systems (CNS) were bioassay in mice. DNA was obtained from all samples (n = 33) and PCR was performed using TOX5-TOX8 T. gondii specific primers. Positive PCR samples were characterized by nested-PCR and restriction fragment length polymorphism using the markers SAG2, BTUB, GRA6, SAG3, PK1, L358, C22-8, C29-2 and Apico. T. gondii DNA was amplified in 30.3% (10/33) of CNS samples. Isolates were obtained in 27.7% (5/18) of inoculated CNS samples (TgCk11-9Arg, TgCk13-5Arg, TgCk14-5Arg, TgCk14-6Arg and TgCk14-7Arg). Seven samples showed a restriction pattern to all markers and were identified as atypical with several alleles type III. Genotyping of T. gondii from samples of patients with retinochoroiditis in the same area could improve the understanding of the epidemiology of toxoplasmosis in the region. Fil: Pardini, Lais Luján. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina Fil: Moré, Gastón Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina Fil: Rudzinski, Marcelo Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de uveítis y glaucoma; Argentina. Universidad Católica de las Misiones; Argentina Fil: Gos, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina Fil: Campero, Lucía María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina Fil: Meyer, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Laboratorio de análisis clínicos Dr Meyer; Argentina Fil: Bernstein, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina Fil: Unzaga, Juan Manuel. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina Fil: Venturini, María Cecilia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
description	The aim of this study was to detect, isolate and genetically characterize Toxoplasma gondii from tissues obtained from free range chickens which were breed in farms from patients with toxoplasmic retinochoroiditis in Misiones, Argentina. Thirty three samples of head (refrigerated = 18 and frozen = 15) from free range chickens were processed. Refrigerated (n = 18) chicken central nervous systems (CNS) were bioassay in mice. DNA was obtained from all samples (n = 33) and PCR was performed using TOX5-TOX8 T. gondii specific primers. Positive PCR samples were characterized by nested-PCR and restriction fragment length polymorphism using the markers SAG2, BTUB, GRA6, SAG3, PK1, L358, C22-8, C29-2 and Apico. T. gondii DNA was amplified in 30.3% (10/33) of CNS samples. Isolates were obtained in 27.7% (5/18) of inoculated CNS samples (TgCk11-9Arg, TgCk13-5Arg, TgCk14-5Arg, TgCk14-6Arg and TgCk14-7Arg). Seven samples showed a restriction pattern to all markers and were identified as atypical with several alleles type III. Genotyping of T. gondii from samples of patients with retinochoroiditis in the same area could improve the understanding of the epidemiology of toxoplasmosis in the region.
publishDate	2016
dc.date.none.fl_str_mv	2016-07
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/54303 Pardini, Lais Luján; Moré, Gastón Andrés; Rudzinski, Marcelo Nicolas; Gos, Maria Laura; Campero, Lucía María; et al.; Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditis; Academic Press Inc Elsevier Science; Experimental Parasitology; 166; 7-2016; 16-20 0014-4894 CONICET Digital CONICET
url	http://hdl.handle.net/11336/54303
identifier_str_mv	Pardini, Lais Luján; Moré, Gastón Andrés; Rudzinski, Marcelo Nicolas; Gos, Maria Laura; Campero, Lucía María; et al.; Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditis; Academic Press Inc Elsevier Science; Experimental Parasitology; 166; 7-2016; 16-20 0014-4894 CONICET Digital CONICET
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repo/semantics/altIdentifier/doi/10.1016/j.exppara.2016.03.006 info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0014489416300327
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv	openAccess
rights_invalid_str_mv	https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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dc.publisher.none.fl_str_mv	Academic Press Inc Elsevier Science
publisher.none.fl_str_mv	Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv	reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas
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repository.mail.fl_str_mv	dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditis

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