Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditis
- Autores
- Pardini, Lais Luján; Moré, Gastón Andrés; Rudzinski, Marcelo Nicolas; Gos, Maria Laura; Campero, Lucía María; Meyer, Alejandro; Bernstein, Mariana; Unzaga, Juan Manuel; Venturini, María Cecilia
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The aim of this study was to detect, isolate and genetically characterize Toxoplasma gondii from tissues obtained from free range chickens which were breed in farms from patients with toxoplasmic retinochoroiditis in Misiones, Argentina. Thirty three samples of head (refrigerated = 18 and frozen = 15) from free range chickens were processed. Refrigerated (n = 18) chicken central nervous systems (CNS) were bioassay in mice. DNA was obtained from all samples (n = 33) and PCR was performed using TOX5-TOX8 T. gondii specific primers. Positive PCR samples were characterized by nested-PCR and restriction fragment length polymorphism using the markers SAG2, BTUB, GRA6, SAG3, PK1, L358, C22-8, C29-2 and Apico. T. gondii DNA was amplified in 30.3% (10/33) of CNS samples. Isolates were obtained in 27.7% (5/18) of inoculated CNS samples (TgCk11-9Arg, TgCk13-5Arg, TgCk14-5Arg, TgCk14-6Arg and TgCk14-7Arg). Seven samples showed a restriction pattern to all markers and were identified as atypical with several alleles type III. Genotyping of T. gondii from samples of patients with retinochoroiditis in the same area could improve the understanding of the epidemiology of toxoplasmosis in the region.
Fil: Pardini, Lais Luján. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Moré, Gastón Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Rudzinski, Marcelo Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de uveítis y glaucoma; Argentina. Universidad Católica de las Misiones; Argentina
Fil: Gos, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Campero, Lucía María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Meyer, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Laboratorio de análisis clínicos Dr Meyer; Argentina
Fil: Bernstein, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Unzaga, Juan Manuel. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Venturini, María Cecilia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina - Materia
-
Free-Range Chickens
Genotyping
Isolates
Retichoroiditis
Toxoplasma Gondii - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/54303
Ver los metadatos del registro completo
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Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditisPardini, Lais LujánMoré, Gastón AndrésRudzinski, Marcelo NicolasGos, Maria LauraCampero, Lucía MaríaMeyer, AlejandroBernstein, MarianaUnzaga, Juan ManuelVenturini, María CeciliaFree-Range ChickensGenotypingIsolatesRetichoroiditisToxoplasma Gondiihttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The aim of this study was to detect, isolate and genetically characterize Toxoplasma gondii from tissues obtained from free range chickens which were breed in farms from patients with toxoplasmic retinochoroiditis in Misiones, Argentina. Thirty three samples of head (refrigerated = 18 and frozen = 15) from free range chickens were processed. Refrigerated (n = 18) chicken central nervous systems (CNS) were bioassay in mice. DNA was obtained from all samples (n = 33) and PCR was performed using TOX5-TOX8 T. gondii specific primers. Positive PCR samples were characterized by nested-PCR and restriction fragment length polymorphism using the markers SAG2, BTUB, GRA6, SAG3, PK1, L358, C22-8, C29-2 and Apico. T. gondii DNA was amplified in 30.3% (10/33) of CNS samples. Isolates were obtained in 27.7% (5/18) of inoculated CNS samples (TgCk11-9Arg, TgCk13-5Arg, TgCk14-5Arg, TgCk14-6Arg and TgCk14-7Arg). Seven samples showed a restriction pattern to all markers and were identified as atypical with several alleles type III. Genotyping of T. gondii from samples of patients with retinochoroiditis in the same area could improve the understanding of the epidemiology of toxoplasmosis in the region.Fil: Pardini, Lais Luján. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; ArgentinaFil: Moré, Gastón Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; ArgentinaFil: Rudzinski, Marcelo Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de uveítis y glaucoma; Argentina. Universidad Católica de las Misiones; ArgentinaFil: Gos, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; ArgentinaFil: Campero, Lucía María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; ArgentinaFil: Meyer, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Laboratorio de análisis clínicos Dr Meyer; ArgentinaFil: Bernstein, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; ArgentinaFil: Unzaga, Juan Manuel. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; ArgentinaFil: Venturini, María Cecilia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; ArgentinaAcademic Press Inc Elsevier Science2016-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/54303Pardini, Lais Luján; Moré, Gastón Andrés; Rudzinski, Marcelo Nicolas; Gos, Maria Laura; Campero, Lucía María; et al.; Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditis; Academic Press Inc Elsevier Science; Experimental Parasitology; 166; 7-2016; 16-200014-4894CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.exppara.2016.03.006info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0014489416300327info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:06Zoai:ri.conicet.gov.ar:11336/54303instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:06.758CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditis |
title |
Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditis |
spellingShingle |
Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditis Pardini, Lais Luján Free-Range Chickens Genotyping Isolates Retichoroiditis Toxoplasma Gondii |
title_short |
Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditis |
title_full |
Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditis |
title_fullStr |
Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditis |
title_full_unstemmed |
Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditis |
title_sort |
Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditis |
dc.creator.none.fl_str_mv |
Pardini, Lais Luján Moré, Gastón Andrés Rudzinski, Marcelo Nicolas Gos, Maria Laura Campero, Lucía María Meyer, Alejandro Bernstein, Mariana Unzaga, Juan Manuel Venturini, María Cecilia |
author |
Pardini, Lais Luján |
author_facet |
Pardini, Lais Luján Moré, Gastón Andrés Rudzinski, Marcelo Nicolas Gos, Maria Laura Campero, Lucía María Meyer, Alejandro Bernstein, Mariana Unzaga, Juan Manuel Venturini, María Cecilia |
author_role |
author |
author2 |
Moré, Gastón Andrés Rudzinski, Marcelo Nicolas Gos, Maria Laura Campero, Lucía María Meyer, Alejandro Bernstein, Mariana Unzaga, Juan Manuel Venturini, María Cecilia |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
Free-Range Chickens Genotyping Isolates Retichoroiditis Toxoplasma Gondii |
topic |
Free-Range Chickens Genotyping Isolates Retichoroiditis Toxoplasma Gondii |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/4.3 https://purl.org/becyt/ford/4 |
dc.description.none.fl_txt_mv |
The aim of this study was to detect, isolate and genetically characterize Toxoplasma gondii from tissues obtained from free range chickens which were breed in farms from patients with toxoplasmic retinochoroiditis in Misiones, Argentina. Thirty three samples of head (refrigerated = 18 and frozen = 15) from free range chickens were processed. Refrigerated (n = 18) chicken central nervous systems (CNS) were bioassay in mice. DNA was obtained from all samples (n = 33) and PCR was performed using TOX5-TOX8 T. gondii specific primers. Positive PCR samples were characterized by nested-PCR and restriction fragment length polymorphism using the markers SAG2, BTUB, GRA6, SAG3, PK1, L358, C22-8, C29-2 and Apico. T. gondii DNA was amplified in 30.3% (10/33) of CNS samples. Isolates were obtained in 27.7% (5/18) of inoculated CNS samples (TgCk11-9Arg, TgCk13-5Arg, TgCk14-5Arg, TgCk14-6Arg and TgCk14-7Arg). Seven samples showed a restriction pattern to all markers and were identified as atypical with several alleles type III. Genotyping of T. gondii from samples of patients with retinochoroiditis in the same area could improve the understanding of the epidemiology of toxoplasmosis in the region. Fil: Pardini, Lais Luján. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina Fil: Moré, Gastón Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina Fil: Rudzinski, Marcelo Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de uveítis y glaucoma; Argentina. Universidad Católica de las Misiones; Argentina Fil: Gos, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina Fil: Campero, Lucía María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina Fil: Meyer, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Laboratorio de análisis clínicos Dr Meyer; Argentina Fil: Bernstein, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina Fil: Unzaga, Juan Manuel. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina Fil: Venturini, María Cecilia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina |
description |
The aim of this study was to detect, isolate and genetically characterize Toxoplasma gondii from tissues obtained from free range chickens which were breed in farms from patients with toxoplasmic retinochoroiditis in Misiones, Argentina. Thirty three samples of head (refrigerated = 18 and frozen = 15) from free range chickens were processed. Refrigerated (n = 18) chicken central nervous systems (CNS) were bioassay in mice. DNA was obtained from all samples (n = 33) and PCR was performed using TOX5-TOX8 T. gondii specific primers. Positive PCR samples were characterized by nested-PCR and restriction fragment length polymorphism using the markers SAG2, BTUB, GRA6, SAG3, PK1, L358, C22-8, C29-2 and Apico. T. gondii DNA was amplified in 30.3% (10/33) of CNS samples. Isolates were obtained in 27.7% (5/18) of inoculated CNS samples (TgCk11-9Arg, TgCk13-5Arg, TgCk14-5Arg, TgCk14-6Arg and TgCk14-7Arg). Seven samples showed a restriction pattern to all markers and were identified as atypical with several alleles type III. Genotyping of T. gondii from samples of patients with retinochoroiditis in the same area could improve the understanding of the epidemiology of toxoplasmosis in the region. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-07 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/54303 Pardini, Lais Luján; Moré, Gastón Andrés; Rudzinski, Marcelo Nicolas; Gos, Maria Laura; Campero, Lucía María; et al.; Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditis; Academic Press Inc Elsevier Science; Experimental Parasitology; 166; 7-2016; 16-20 0014-4894 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/54303 |
identifier_str_mv |
Pardini, Lais Luján; Moré, Gastón Andrés; Rudzinski, Marcelo Nicolas; Gos, Maria Laura; Campero, Lucía María; et al.; Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditis; Academic Press Inc Elsevier Science; Experimental Parasitology; 166; 7-2016; 16-20 0014-4894 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.exppara.2016.03.006 info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0014489416300327 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Academic Press Inc Elsevier Science |
publisher.none.fl_str_mv |
Academic Press Inc Elsevier Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.13397 |