Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditis

Autores
Pardini, Lais Luján; Moré, Gastón Andrés; Rudzinski, Marcelo Nicolas; Gos, Maria Laura; Campero, Lucía María; Meyer, Alejandro; Bernstein, Mariana; Unzaga, Juan Manuel; Venturini, María Cecilia
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to detect, isolate and genetically characterize Toxoplasma gondii from tissues obtained from free range chickens which were breed in farms from patients with toxoplasmic retinochoroiditis in Misiones, Argentina. Thirty three samples of head (refrigerated = 18 and frozen = 15) from free range chickens were processed. Refrigerated (n = 18) chicken central nervous systems (CNS) were bioassay in mice. DNA was obtained from all samples (n = 33) and PCR was performed using TOX5-TOX8 T. gondii specific primers. Positive PCR samples were characterized by nested-PCR and restriction fragment length polymorphism using the markers SAG2, BTUB, GRA6, SAG3, PK1, L358, C22-8, C29-2 and Apico. T. gondii DNA was amplified in 30.3% (10/33) of CNS samples. Isolates were obtained in 27.7% (5/18) of inoculated CNS samples (TgCk11-9Arg, TgCk13-5Arg, TgCk14-5Arg, TgCk14-6Arg and TgCk14-7Arg). Seven samples showed a restriction pattern to all markers and were identified as atypical with several alleles type III. Genotyping of T. gondii from samples of patients with retinochoroiditis in the same area could improve the understanding of the epidemiology of toxoplasmosis in the region.
Fil: Pardini, Lais Luján. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Moré, Gastón Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Rudzinski, Marcelo Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de uveítis y glaucoma; Argentina. Universidad Católica de las Misiones; Argentina
Fil: Gos, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Campero, Lucía María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Meyer, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Laboratorio de análisis clínicos Dr Meyer; Argentina
Fil: Bernstein, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Unzaga, Juan Manuel. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Venturini, María Cecilia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Materia
Free-Range Chickens
Genotyping
Isolates
Retichoroiditis
Toxoplasma Gondii
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/54303

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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditisPardini, Lais LujánMoré, Gastón AndrésRudzinski, Marcelo NicolasGos, Maria LauraCampero, Lucía MaríaMeyer, AlejandroBernstein, MarianaUnzaga, Juan ManuelVenturini, María CeciliaFree-Range ChickensGenotypingIsolatesRetichoroiditisToxoplasma Gondiihttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The aim of this study was to detect, isolate and genetically characterize Toxoplasma gondii from tissues obtained from free range chickens which were breed in farms from patients with toxoplasmic retinochoroiditis in Misiones, Argentina. Thirty three samples of head (refrigerated = 18 and frozen = 15) from free range chickens were processed. Refrigerated (n = 18) chicken central nervous systems (CNS) were bioassay in mice. DNA was obtained from all samples (n = 33) and PCR was performed using TOX5-TOX8 T. gondii specific primers. Positive PCR samples were characterized by nested-PCR and restriction fragment length polymorphism using the markers SAG2, BTUB, GRA6, SAG3, PK1, L358, C22-8, C29-2 and Apico. T. gondii DNA was amplified in 30.3% (10/33) of CNS samples. Isolates were obtained in 27.7% (5/18) of inoculated CNS samples (TgCk11-9Arg, TgCk13-5Arg, TgCk14-5Arg, TgCk14-6Arg and TgCk14-7Arg). Seven samples showed a restriction pattern to all markers and were identified as atypical with several alleles type III. Genotyping of T. gondii from samples of patients with retinochoroiditis in the same area could improve the understanding of the epidemiology of toxoplasmosis in the region.Fil: Pardini, Lais Luján. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; ArgentinaFil: Moré, Gastón Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; ArgentinaFil: Rudzinski, Marcelo Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de uveítis y glaucoma; Argentina. Universidad Católica de las Misiones; ArgentinaFil: Gos, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; ArgentinaFil: Campero, Lucía María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; ArgentinaFil: Meyer, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Laboratorio de análisis clínicos Dr Meyer; ArgentinaFil: Bernstein, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; ArgentinaFil: Unzaga, Juan Manuel. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; ArgentinaFil: Venturini, María Cecilia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; ArgentinaAcademic Press Inc Elsevier Science2016-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/54303Pardini, Lais Luján; Moré, Gastón Andrés; Rudzinski, Marcelo Nicolas; Gos, Maria Laura; Campero, Lucía María; et al.; Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditis; Academic Press Inc Elsevier Science; Experimental Parasitology; 166; 7-2016; 16-200014-4894CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.exppara.2016.03.006info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0014489416300327info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:06Zoai:ri.conicet.gov.ar:11336/54303instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:06.758CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditis
title Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditis
spellingShingle Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditis
Pardini, Lais Luján
Free-Range Chickens
Genotyping
Isolates
Retichoroiditis
Toxoplasma Gondii
title_short Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditis
title_full Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditis
title_fullStr Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditis
title_full_unstemmed Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditis
title_sort Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditis
dc.creator.none.fl_str_mv Pardini, Lais Luján
Moré, Gastón Andrés
Rudzinski, Marcelo Nicolas
Gos, Maria Laura
Campero, Lucía María
Meyer, Alejandro
Bernstein, Mariana
Unzaga, Juan Manuel
Venturini, María Cecilia
author Pardini, Lais Luján
author_facet Pardini, Lais Luján
Moré, Gastón Andrés
Rudzinski, Marcelo Nicolas
Gos, Maria Laura
Campero, Lucía María
Meyer, Alejandro
Bernstein, Mariana
Unzaga, Juan Manuel
Venturini, María Cecilia
author_role author
author2 Moré, Gastón Andrés
Rudzinski, Marcelo Nicolas
Gos, Maria Laura
Campero, Lucía María
Meyer, Alejandro
Bernstein, Mariana
Unzaga, Juan Manuel
Venturini, María Cecilia
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Free-Range Chickens
Genotyping
Isolates
Retichoroiditis
Toxoplasma Gondii
topic Free-Range Chickens
Genotyping
Isolates
Retichoroiditis
Toxoplasma Gondii
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The aim of this study was to detect, isolate and genetically characterize Toxoplasma gondii from tissues obtained from free range chickens which were breed in farms from patients with toxoplasmic retinochoroiditis in Misiones, Argentina. Thirty three samples of head (refrigerated = 18 and frozen = 15) from free range chickens were processed. Refrigerated (n = 18) chicken central nervous systems (CNS) were bioassay in mice. DNA was obtained from all samples (n = 33) and PCR was performed using TOX5-TOX8 T. gondii specific primers. Positive PCR samples were characterized by nested-PCR and restriction fragment length polymorphism using the markers SAG2, BTUB, GRA6, SAG3, PK1, L358, C22-8, C29-2 and Apico. T. gondii DNA was amplified in 30.3% (10/33) of CNS samples. Isolates were obtained in 27.7% (5/18) of inoculated CNS samples (TgCk11-9Arg, TgCk13-5Arg, TgCk14-5Arg, TgCk14-6Arg and TgCk14-7Arg). Seven samples showed a restriction pattern to all markers and were identified as atypical with several alleles type III. Genotyping of T. gondii from samples of patients with retinochoroiditis in the same area could improve the understanding of the epidemiology of toxoplasmosis in the region.
Fil: Pardini, Lais Luján. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Moré, Gastón Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Rudzinski, Marcelo Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de uveítis y glaucoma; Argentina. Universidad Católica de las Misiones; Argentina
Fil: Gos, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Campero, Lucía María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Meyer, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Laboratorio de análisis clínicos Dr Meyer; Argentina
Fil: Bernstein, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Unzaga, Juan Manuel. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Venturini, María Cecilia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
description The aim of this study was to detect, isolate and genetically characterize Toxoplasma gondii from tissues obtained from free range chickens which were breed in farms from patients with toxoplasmic retinochoroiditis in Misiones, Argentina. Thirty three samples of head (refrigerated = 18 and frozen = 15) from free range chickens were processed. Refrigerated (n = 18) chicken central nervous systems (CNS) were bioassay in mice. DNA was obtained from all samples (n = 33) and PCR was performed using TOX5-TOX8 T. gondii specific primers. Positive PCR samples were characterized by nested-PCR and restriction fragment length polymorphism using the markers SAG2, BTUB, GRA6, SAG3, PK1, L358, C22-8, C29-2 and Apico. T. gondii DNA was amplified in 30.3% (10/33) of CNS samples. Isolates were obtained in 27.7% (5/18) of inoculated CNS samples (TgCk11-9Arg, TgCk13-5Arg, TgCk14-5Arg, TgCk14-6Arg and TgCk14-7Arg). Seven samples showed a restriction pattern to all markers and were identified as atypical with several alleles type III. Genotyping of T. gondii from samples of patients with retinochoroiditis in the same area could improve the understanding of the epidemiology of toxoplasmosis in the region.
publishDate 2016
dc.date.none.fl_str_mv 2016-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/54303
Pardini, Lais Luján; Moré, Gastón Andrés; Rudzinski, Marcelo Nicolas; Gos, Maria Laura; Campero, Lucía María; et al.; Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditis; Academic Press Inc Elsevier Science; Experimental Parasitology; 166; 7-2016; 16-20
0014-4894
CONICET Digital
CONICET
url http://hdl.handle.net/11336/54303
identifier_str_mv Pardini, Lais Luján; Moré, Gastón Andrés; Rudzinski, Marcelo Nicolas; Gos, Maria Laura; Campero, Lucía María; et al.; Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditis; Academic Press Inc Elsevier Science; Experimental Parasitology; 166; 7-2016; 16-20
0014-4894
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.exppara.2016.03.006
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0014489416300327
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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