Toxoplasma gondii infection in sentinel and free-range chickens from Argentina

Autores
Moré, Gastón Andrés; Maksimov, P.; Pardini, Lais Luján; Herrmann, D. C.; Bacigalupe, Diana; Maksimov, A.; Basso, Walter Ubaldo; Conraths, F.J.; Schares, G.; Venturini, María Cecilia
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study aimed at isolating and genotyping Toxoplasma gondii from serologically positivefree-range chickens from Argentina, and to evaluate the use of sentinel animals during ashort time period of exposure to determine environmental contamination with T. gondiioocysts. Two groups of chickens on six farms were compared in this study: (i) young, 2?3month-old broiler-type chickens reared as sentinel animals on the farms and (ii) adultchickens reared on the same farms for more than one year. Seroconversion rates of 7.0% or5.7% were observed in sentinel broiler chickens reared for a period of 74 days (January?April2010) or 88 days (August?November 2010) respectively, as shown by a T. gondii specificimmunofluorescent antibody test. Fifty-three percent(17 of 32) of adult chickens were positive and showed higher titres than sentinel animals. Isolation of T. gondiifrom tissues (brainand heart) of serologically positive chickens was achieved from six of seven free-range adultbirds with IFAT titres of 200 and higher. The isolated parasites were analysed by multi-locuspolymerase chain reaction?restriction fragmentlength polymorphism (PCR-RFLP). The isolated T. gondii showed three different genotypes: two genotypes consisted in atypical allelecombinations, and the remaining genotype had exclusively clonal type II alleles. All isolates obtained at a single farm, corresponded to the same genotype. The T. gondii genotypesobserved are identical to those described in cats, dogs, chickens and capybaras elsewherein South America. Two isolates, which showed different allele combinations in PCR-RFLP,were characterized in a mouse virulence assay. While one isolate showed a low virulencea second isolate was of intermediate virulence to mice.
Fil: Moré, Gastón Andrés. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Maksimov, P.. Federal Research Institute for Animal Health; Alemania
Fil: Pardini, Lais Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Herrmann, D. C.. Federal Research Institute for Animal Health; Alemania
Fil: Bacigalupe, Diana. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Maksimov, A.. Federal Research Institute for Animal Health; Alemania
Fil: Basso, Walter Ubaldo. Universitat Zurich; Suiza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Conraths, F.J.. Federal Research Institute for Animal Health; Alemania
Fil: Schares, G.. Federal Research Institute for Animal Health; Alemania
Fil: Venturini, María Cecilia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Materia
TOXOPLASMA GONDII
CHICKEN
GENOTYPE
MULTI-LOCUS GENOTYPING
SENTINEL
VIRULENCE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/273209
Acceder
id CONICETDig_081a3ec30d42b3f8efe0fe15ab0c3c78
oai_identifier_str oai:ri.conicet.gov.ar:11336/273209
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Toxoplasma gondii infection in sentinel and free-range chickens from ArgentinaMoré, Gastón AndrésMaksimov, P.Pardini, Lais LujánHerrmann, D. C.Bacigalupe, DianaMaksimov, A.Basso, Walter UbaldoConraths, F.J.Schares, G.Venturini, María CeciliaTOXOPLASMA GONDIICHICKENGENOTYPEMULTI-LOCUS GENOTYPINGSENTINELVIRULENCEhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4This study aimed at isolating and genotyping Toxoplasma gondii from serologically positivefree-range chickens from Argentina, and to evaluate the use of sentinel animals during ashort time period of exposure to determine environmental contamination with T. gondiioocysts. Two groups of chickens on six farms were compared in this study: (i) young, 2?3month-old broiler-type chickens reared as sentinel animals on the farms and (ii) adultchickens reared on the same farms for more than one year. Seroconversion rates of 7.0% or5.7% were observed in sentinel broiler chickens reared for a period of 74 days (January?April2010) or 88 days (August?November 2010) respectively, as shown by a T. gondii specificimmunofluorescent antibody test. Fifty-three percent(17 of 32) of adult chickens were positive and showed higher titres than sentinel animals. Isolation of T. gondiifrom tissues (brainand heart) of serologically positive chickens was achieved from six of seven free-range adultbirds with IFAT titres of 200 and higher. The isolated parasites were analysed by multi-locuspolymerase chain reaction?restriction fragmentlength polymorphism (PCR-RFLP). The isolated T. gondii showed three different genotypes: two genotypes consisted in atypical allelecombinations, and the remaining genotype had exclusively clonal type II alleles. All isolates obtained at a single farm, corresponded to the same genotype. The T. gondii genotypesobserved are identical to those described in cats, dogs, chickens and capybaras elsewherein South America. Two isolates, which showed different allele combinations in PCR-RFLP,were characterized in a mouse virulence assay. While one isolate showed a low virulencea second isolate was of intermediate virulence to mice.Fil: Moré, Gastón Andrés. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Maksimov, P.. Federal Research Institute for Animal Health; AlemaniaFil: Pardini, Lais Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; ArgentinaFil: Herrmann, D. C.. Federal Research Institute for Animal Health; AlemaniaFil: Bacigalupe, Diana. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; ArgentinaFil: Maksimov, A.. Federal Research Institute for Animal Health; AlemaniaFil: Basso, Walter Ubaldo. Universitat Zurich; Suiza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; ArgentinaFil: Conraths, F.J.. Federal Research Institute for Animal Health; AlemaniaFil: Schares, G.. Federal Research Institute for Animal Health; AlemaniaFil: Venturini, María Cecilia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; ArgentinaElsevier Science2012-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/273209Moré, Gastón Andrés; Maksimov, P.; Pardini, Lais Luján; Herrmann, D. C.; Bacigalupe, Diana; et al.; Toxoplasma gondii infection in sentinel and free-range chickens from Argentina; Elsevier Science; Veterinary Parasitology; 184; 2-4; 3-2012; 116-1210304-4017CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0304401711006145info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetpar.2011.09.012info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:23:22Zoai:ri.conicet.gov.ar:11336/273209instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:23:22.889CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Toxoplasma gondii infection in sentinel and free-range chickens from Argentina
title Toxoplasma gondii infection in sentinel and free-range chickens from Argentina
spellingShingle Toxoplasma gondii infection in sentinel and free-range chickens from Argentina
Moré, Gastón Andrés
TOXOPLASMA GONDII
CHICKEN
GENOTYPE
MULTI-LOCUS GENOTYPING
SENTINEL
VIRULENCE
title_short Toxoplasma gondii infection in sentinel and free-range chickens from Argentina
title_full Toxoplasma gondii infection in sentinel and free-range chickens from Argentina
title_fullStr Toxoplasma gondii infection in sentinel and free-range chickens from Argentina
title_full_unstemmed Toxoplasma gondii infection in sentinel and free-range chickens from Argentina
title_sort Toxoplasma gondii infection in sentinel and free-range chickens from Argentina
dc.creator.none.fl_str_mv Moré, Gastón Andrés
Maksimov, P.
Pardini, Lais Luján
Herrmann, D. C.
Bacigalupe, Diana
Maksimov, A.
Basso, Walter Ubaldo
Conraths, F.J.
Schares, G.
Venturini, María Cecilia
author Moré, Gastón Andrés
author_facet Moré, Gastón Andrés
Maksimov, P.
Pardini, Lais Luján
Herrmann, D. C.
Bacigalupe, Diana
Maksimov, A.
Basso, Walter Ubaldo
Conraths, F.J.
Schares, G.
Venturini, María Cecilia
author_role author
author2 Maksimov, P.
Pardini, Lais Luján
Herrmann, D. C.
Bacigalupe, Diana
Maksimov, A.
Basso, Walter Ubaldo
Conraths, F.J.
Schares, G.
Venturini, María Cecilia
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv TOXOPLASMA GONDII
CHICKEN
GENOTYPE
MULTI-LOCUS GENOTYPING
SENTINEL
VIRULENCE
topic TOXOPLASMA GONDII
CHICKEN
GENOTYPE
MULTI-LOCUS GENOTYPING
SENTINEL
VIRULENCE
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv This study aimed at isolating and genotyping Toxoplasma gondii from serologically positivefree-range chickens from Argentina, and to evaluate the use of sentinel animals during ashort time period of exposure to determine environmental contamination with T. gondiioocysts. Two groups of chickens on six farms were compared in this study: (i) young, 2?3month-old broiler-type chickens reared as sentinel animals on the farms and (ii) adultchickens reared on the same farms for more than one year. Seroconversion rates of 7.0% or5.7% were observed in sentinel broiler chickens reared for a period of 74 days (January?April2010) or 88 days (August?November 2010) respectively, as shown by a T. gondii specificimmunofluorescent antibody test. Fifty-three percent(17 of 32) of adult chickens were positive and showed higher titres than sentinel animals. Isolation of T. gondiifrom tissues (brainand heart) of serologically positive chickens was achieved from six of seven free-range adultbirds with IFAT titres of 200 and higher. The isolated parasites were analysed by multi-locuspolymerase chain reaction?restriction fragmentlength polymorphism (PCR-RFLP). The isolated T. gondii showed three different genotypes: two genotypes consisted in atypical allelecombinations, and the remaining genotype had exclusively clonal type II alleles. All isolates obtained at a single farm, corresponded to the same genotype. The T. gondii genotypesobserved are identical to those described in cats, dogs, chickens and capybaras elsewherein South America. Two isolates, which showed different allele combinations in PCR-RFLP,were characterized in a mouse virulence assay. While one isolate showed a low virulencea second isolate was of intermediate virulence to mice.
Fil: Moré, Gastón Andrés. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Maksimov, P.. Federal Research Institute for Animal Health; Alemania
Fil: Pardini, Lais Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Herrmann, D. C.. Federal Research Institute for Animal Health; Alemania
Fil: Bacigalupe, Diana. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Maksimov, A.. Federal Research Institute for Animal Health; Alemania
Fil: Basso, Walter Ubaldo. Universitat Zurich; Suiza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Conraths, F.J.. Federal Research Institute for Animal Health; Alemania
Fil: Schares, G.. Federal Research Institute for Animal Health; Alemania
Fil: Venturini, María Cecilia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
description This study aimed at isolating and genotyping Toxoplasma gondii from serologically positivefree-range chickens from Argentina, and to evaluate the use of sentinel animals during ashort time period of exposure to determine environmental contamination with T. gondiioocysts. Two groups of chickens on six farms were compared in this study: (i) young, 2?3month-old broiler-type chickens reared as sentinel animals on the farms and (ii) adultchickens reared on the same farms for more than one year. Seroconversion rates of 7.0% or5.7% were observed in sentinel broiler chickens reared for a period of 74 days (January?April2010) or 88 days (August?November 2010) respectively, as shown by a T. gondii specificimmunofluorescent antibody test. Fifty-three percent(17 of 32) of adult chickens were positive and showed higher titres than sentinel animals. Isolation of T. gondiifrom tissues (brainand heart) of serologically positive chickens was achieved from six of seven free-range adultbirds with IFAT titres of 200 and higher. The isolated parasites were analysed by multi-locuspolymerase chain reaction?restriction fragmentlength polymorphism (PCR-RFLP). The isolated T. gondii showed three different genotypes: two genotypes consisted in atypical allelecombinations, and the remaining genotype had exclusively clonal type II alleles. All isolates obtained at a single farm, corresponded to the same genotype. The T. gondii genotypesobserved are identical to those described in cats, dogs, chickens and capybaras elsewherein South America. Two isolates, which showed different allele combinations in PCR-RFLP,were characterized in a mouse virulence assay. While one isolate showed a low virulencea second isolate was of intermediate virulence to mice.
publishDate 2012
dc.date.none.fl_str_mv 2012-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/273209
Moré, Gastón Andrés; Maksimov, P.; Pardini, Lais Luján; Herrmann, D. C.; Bacigalupe, Diana; et al.; Toxoplasma gondii infection in sentinel and free-range chickens from Argentina; Elsevier Science; Veterinary Parasitology; 184; 2-4; 3-2012; 116-121
0304-4017
CONICET Digital
CONICET
url http://hdl.handle.net/11336/273209
identifier_str_mv Moré, Gastón Andrés; Maksimov, P.; Pardini, Lais Luján; Herrmann, D. C.; Bacigalupe, Diana; et al.; Toxoplasma gondii infection in sentinel and free-range chickens from Argentina; Elsevier Science; Veterinary Parasitology; 184; 2-4; 3-2012; 116-121
0304-4017
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0304401711006145
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetpar.2011.09.012
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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