<i>Toxoplasma gondii</i> and <i>Neospora caninum</i> infections in synanthropic rodents from Argentina

Autores
Dellarupe, Andrea; Fitte, Bruno; Pardini, Lais; Campero, Lucía María; Bernstein, Mariana; Robles, María del Rosario; Moré, Gastón Andrés; Venturini, María Cecilia; Unzaga, Juan Manuel
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Toxoplasma gondii and Neospora caninum are closely related coccidian parasites (phylum Apicomplexa). This is the first study from urban synanthropic rodent species that involved serological and molecular diagnosis of T. gondii and N. caninum infection, and genotyping of T. gondii in Argentina. A total of 127 rodent samples were trap captured: Mus musculus (n = 78), Rattus norvegicus (n = 26) and Rattus rattus (n = 23). Antibodies against T. gondii and N. caninum were detected by IFAT in 32.8% (40/122) and 0.8% (1/122) of rodent samples, respectively, demonstrating contact with these protozoans. Additionally, T. gondii DNA was detected in 3.3% (4/123) of rodent central nervous system samples and 2 samples were genotyped by multilocus nPCR-RFLP. Neospora caninum DNA was not detected by PCR. The 2 genotyped samples were type III allele for all markers except for SAG-1 (type I for Rat1Arg and type II/III for Rat2Arg) and were identified as #48 and #2 (likely) according to the allele combinations reported on Toxo DB (Toxo-DB). The results of the present study revealed a wide distribution of T. gondii and less for N. caninum, in synanthropic rats and mice in the studied area.
Toxoplasma gondii e Neospora caninum são parasitas coccídeos intimamente relacionados (filo Apicomplexa). Este é o primeiro estudo de espécies de roedores sinantrópicos urbanos, o qual envolveu diagnósticos sorológicos e moleculares da infecção por T. gondii e N. caninum e genotipagem de T. gondii na Argentina. Um total de 127 amostras de roedores foram obtidas: Mus musculus (n = 78), Rattus norvegicus (n = 26) e Rattus rattus (n = 23). Anticorpos contra T. gondii e N. caninum foram detectados pela IFAT em 32,8% (40/122) e 0,8% (1/122) das amostras de roedores, respectivamente, demonstrando contato com esses protozoários. Adicionalmente, o DNA de T. gondii foi detectado em 3,3% (4/123) das amostras do sistema nervoso central de roedores e duas amostras foram genotipadas por nPCR-RFLP multilocus. O DNA de N. caninum não foi detectado por PCR. As 2 amostras genotipadas eram do tipo III para todos os marcadores, exceto para SAG-1 (tipo I para Rat1Arg e tipo II / III para Rat2Arg) e foram identificadas como # 48 e # 2 (provavelmente) de acordo com as combinações de alelos relatadas no Toxo DB (Toxo-DB). Os resultados do presente estudo indicam uma ampla distribuição de T. gondii e menor para N. caninum, em ratos e camundongos sinantrópicos na área estudada.
Facultad de Ciencias Veterinarias
Centro de Estudios Parasitológicos y de Vectores
Materia
Ciencias Veterinarias
Toxoplasma gondii
Neospora caninum
Synanthropic rodent
Serology
Genotyping
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/123713

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network_name_str SEDICI (UNLP)
spelling <i>Toxoplasma gondii</i> and <i>Neospora caninum</i> infections in synanthropic rodents from ArgentinaInfecções por Toxoplasma gondii e Neospora caninum em roedores sinantrópicos da ArgentinaDellarupe, AndreaFitte, BrunoPardini, LaisCampero, Lucía MaríaBernstein, MarianaRobles, María del RosarioMoré, Gastón AndrésVenturini, María CeciliaUnzaga, Juan ManuelCiencias VeterinariasToxoplasma gondiiNeospora caninumSynanthropic rodentSerologyGenotypingToxoplasma gondii and Neospora caninum are closely related coccidian parasites (phylum Apicomplexa). This is the first study from urban synanthropic rodent species that involved serological and molecular diagnosis of T. gondii and N. caninum infection, and genotyping of T. gondii in Argentina. A total of 127 rodent samples were trap captured: Mus musculus (n = 78), Rattus norvegicus (n = 26) and Rattus rattus (n = 23). Antibodies against T. gondii and N. caninum were detected by IFAT in 32.8% (40/122) and 0.8% (1/122) of rodent samples, respectively, demonstrating contact with these protozoans. Additionally, T. gondii DNA was detected in 3.3% (4/123) of rodent central nervous system samples and 2 samples were genotyped by multilocus nPCR-RFLP. Neospora caninum DNA was not detected by PCR. The 2 genotyped samples were type III allele for all markers except for SAG-1 (type I for Rat1Arg and type II/III for Rat2Arg) and were identified as #48 and #2 (likely) according to the allele combinations reported on Toxo DB (Toxo-DB). The results of the present study revealed a wide distribution of T. gondii and less for N. caninum, in synanthropic rats and mice in the studied area.Toxoplasma gondii e Neospora caninum são parasitas coccídeos intimamente relacionados (filo Apicomplexa). Este é o primeiro estudo de espécies de roedores sinantrópicos urbanos, o qual envolveu diagnósticos sorológicos e moleculares da infecção por T. gondii e N. caninum e genotipagem de T. gondii na Argentina. Um total de 127 amostras de roedores foram obtidas: Mus musculus (n = 78), Rattus norvegicus (n = 26) e Rattus rattus (n = 23). Anticorpos contra T. gondii e N. caninum foram detectados pela IFAT em 32,8% (40/122) e 0,8% (1/122) das amostras de roedores, respectivamente, demonstrando contato com esses protozoários. Adicionalmente, o DNA de T. gondii foi detectado em 3,3% (4/123) das amostras do sistema nervoso central de roedores e duas amostras foram genotipadas por nPCR-RFLP multilocus. O DNA de N. caninum não foi detectado por PCR. As 2 amostras genotipadas eram do tipo III para todos os marcadores, exceto para SAG-1 (tipo I para Rat1Arg e tipo II / III para Rat2Arg) e foram identificadas como # 48 e # 2 (provavelmente) de acordo com as combinações de alelos relatadas no Toxo DB (Toxo-DB). Os resultados do presente estudo indicam uma ampla distribuição de T. gondii e menor para N. caninum, em ratos e camundongos sinantrópicos na área estudada.Facultad de Ciencias VeterinariasCentro de Estudios Parasitológicos y de Vectores2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf113-118http://sedici.unlp.edu.ar/handle/10915/123713enginfo:eu-repo/semantics/altIdentifier/issn/1984-2961info:eu-repo/semantics/altIdentifier/issn/0103-846Xinfo:eu-repo/semantics/altIdentifier/pmid/30916257info:eu-repo/semantics/altIdentifier/doi/10.1590/s1984-29612019009info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T11:01:33Zoai:sedici.unlp.edu.ar:10915/123713Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 11:01:34.154SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv <i>Toxoplasma gondii</i> and <i>Neospora caninum</i> infections in synanthropic rodents from Argentina
Infecções por Toxoplasma gondii e Neospora caninum em roedores sinantrópicos da Argentina
title <i>Toxoplasma gondii</i> and <i>Neospora caninum</i> infections in synanthropic rodents from Argentina
spellingShingle <i>Toxoplasma gondii</i> and <i>Neospora caninum</i> infections in synanthropic rodents from Argentina
Dellarupe, Andrea
Ciencias Veterinarias
Toxoplasma gondii
Neospora caninum
Synanthropic rodent
Serology
Genotyping
title_short <i>Toxoplasma gondii</i> and <i>Neospora caninum</i> infections in synanthropic rodents from Argentina
title_full <i>Toxoplasma gondii</i> and <i>Neospora caninum</i> infections in synanthropic rodents from Argentina
title_fullStr <i>Toxoplasma gondii</i> and <i>Neospora caninum</i> infections in synanthropic rodents from Argentina
title_full_unstemmed <i>Toxoplasma gondii</i> and <i>Neospora caninum</i> infections in synanthropic rodents from Argentina
title_sort <i>Toxoplasma gondii</i> and <i>Neospora caninum</i> infections in synanthropic rodents from Argentina
dc.creator.none.fl_str_mv Dellarupe, Andrea
Fitte, Bruno
Pardini, Lais
Campero, Lucía María
Bernstein, Mariana
Robles, María del Rosario
Moré, Gastón Andrés
Venturini, María Cecilia
Unzaga, Juan Manuel
author Dellarupe, Andrea
author_facet Dellarupe, Andrea
Fitte, Bruno
Pardini, Lais
Campero, Lucía María
Bernstein, Mariana
Robles, María del Rosario
Moré, Gastón Andrés
Venturini, María Cecilia
Unzaga, Juan Manuel
author_role author
author2 Fitte, Bruno
Pardini, Lais
Campero, Lucía María
Bernstein, Mariana
Robles, María del Rosario
Moré, Gastón Andrés
Venturini, María Cecilia
Unzaga, Juan Manuel
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
Toxoplasma gondii
Neospora caninum
Synanthropic rodent
Serology
Genotyping
topic Ciencias Veterinarias
Toxoplasma gondii
Neospora caninum
Synanthropic rodent
Serology
Genotyping
dc.description.none.fl_txt_mv Toxoplasma gondii and Neospora caninum are closely related coccidian parasites (phylum Apicomplexa). This is the first study from urban synanthropic rodent species that involved serological and molecular diagnosis of T. gondii and N. caninum infection, and genotyping of T. gondii in Argentina. A total of 127 rodent samples were trap captured: Mus musculus (n = 78), Rattus norvegicus (n = 26) and Rattus rattus (n = 23). Antibodies against T. gondii and N. caninum were detected by IFAT in 32.8% (40/122) and 0.8% (1/122) of rodent samples, respectively, demonstrating contact with these protozoans. Additionally, T. gondii DNA was detected in 3.3% (4/123) of rodent central nervous system samples and 2 samples were genotyped by multilocus nPCR-RFLP. Neospora caninum DNA was not detected by PCR. The 2 genotyped samples were type III allele for all markers except for SAG-1 (type I for Rat1Arg and type II/III for Rat2Arg) and were identified as #48 and #2 (likely) according to the allele combinations reported on Toxo DB (Toxo-DB). The results of the present study revealed a wide distribution of T. gondii and less for N. caninum, in synanthropic rats and mice in the studied area.
Toxoplasma gondii e Neospora caninum são parasitas coccídeos intimamente relacionados (filo Apicomplexa). Este é o primeiro estudo de espécies de roedores sinantrópicos urbanos, o qual envolveu diagnósticos sorológicos e moleculares da infecção por T. gondii e N. caninum e genotipagem de T. gondii na Argentina. Um total de 127 amostras de roedores foram obtidas: Mus musculus (n = 78), Rattus norvegicus (n = 26) e Rattus rattus (n = 23). Anticorpos contra T. gondii e N. caninum foram detectados pela IFAT em 32,8% (40/122) e 0,8% (1/122) das amostras de roedores, respectivamente, demonstrando contato com esses protozoários. Adicionalmente, o DNA de T. gondii foi detectado em 3,3% (4/123) das amostras do sistema nervoso central de roedores e duas amostras foram genotipadas por nPCR-RFLP multilocus. O DNA de N. caninum não foi detectado por PCR. As 2 amostras genotipadas eram do tipo III para todos os marcadores, exceto para SAG-1 (tipo I para Rat1Arg e tipo II / III para Rat2Arg) e foram identificadas como # 48 e # 2 (provavelmente) de acordo com as combinações de alelos relatadas no Toxo DB (Toxo-DB). Os resultados do presente estudo indicam uma ampla distribuição de T. gondii e menor para N. caninum, em ratos e camundongos sinantrópicos na área estudada.
Facultad de Ciencias Veterinarias
Centro de Estudios Parasitológicos y de Vectores
description Toxoplasma gondii and Neospora caninum are closely related coccidian parasites (phylum Apicomplexa). This is the first study from urban synanthropic rodent species that involved serological and molecular diagnosis of T. gondii and N. caninum infection, and genotyping of T. gondii in Argentina. A total of 127 rodent samples were trap captured: Mus musculus (n = 78), Rattus norvegicus (n = 26) and Rattus rattus (n = 23). Antibodies against T. gondii and N. caninum were detected by IFAT in 32.8% (40/122) and 0.8% (1/122) of rodent samples, respectively, demonstrating contact with these protozoans. Additionally, T. gondii DNA was detected in 3.3% (4/123) of rodent central nervous system samples and 2 samples were genotyped by multilocus nPCR-RFLP. Neospora caninum DNA was not detected by PCR. The 2 genotyped samples were type III allele for all markers except for SAG-1 (type I for Rat1Arg and type II/III for Rat2Arg) and were identified as #48 and #2 (likely) according to the allele combinations reported on Toxo DB (Toxo-DB). The results of the present study revealed a wide distribution of T. gondii and less for N. caninum, in synanthropic rats and mice in the studied area.
publishDate 2019
dc.date.none.fl_str_mv 2019
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info:eu-repo/semantics/altIdentifier/doi/10.1590/s1984-29612019009
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
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