<i>Toxoplasma gondii</i> and <i>Neospora caninum</i> infections in synanthropic rodents from Argentina
- Autores
- Dellarupe, Andrea; Fitte, Bruno; Pardini, Lais; Campero, Lucía María; Bernstein, Mariana; Robles, María del Rosario; Moré, Gastón Andrés; Venturini, María Cecilia; Unzaga, Juan Manuel
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Toxoplasma gondii and Neospora caninum are closely related coccidian parasites (phylum Apicomplexa). This is the first study from urban synanthropic rodent species that involved serological and molecular diagnosis of T. gondii and N. caninum infection, and genotyping of T. gondii in Argentina. A total of 127 rodent samples were trap captured: Mus musculus (n = 78), Rattus norvegicus (n = 26) and Rattus rattus (n = 23). Antibodies against T. gondii and N. caninum were detected by IFAT in 32.8% (40/122) and 0.8% (1/122) of rodent samples, respectively, demonstrating contact with these protozoans. Additionally, T. gondii DNA was detected in 3.3% (4/123) of rodent central nervous system samples and 2 samples were genotyped by multilocus nPCR-RFLP. Neospora caninum DNA was not detected by PCR. The 2 genotyped samples were type III allele for all markers except for SAG-1 (type I for Rat1Arg and type II/III for Rat2Arg) and were identified as #48 and #2 (likely) according to the allele combinations reported on Toxo DB (Toxo-DB). The results of the present study revealed a wide distribution of T. gondii and less for N. caninum, in synanthropic rats and mice in the studied area.
Toxoplasma gondii e Neospora caninum são parasitas coccídeos intimamente relacionados (filo Apicomplexa). Este é o primeiro estudo de espécies de roedores sinantrópicos urbanos, o qual envolveu diagnósticos sorológicos e moleculares da infecção por T. gondii e N. caninum e genotipagem de T. gondii na Argentina. Um total de 127 amostras de roedores foram obtidas: Mus musculus (n = 78), Rattus norvegicus (n = 26) e Rattus rattus (n = 23). Anticorpos contra T. gondii e N. caninum foram detectados pela IFAT em 32,8% (40/122) e 0,8% (1/122) das amostras de roedores, respectivamente, demonstrando contato com esses protozoários. Adicionalmente, o DNA de T. gondii foi detectado em 3,3% (4/123) das amostras do sistema nervoso central de roedores e duas amostras foram genotipadas por nPCR-RFLP multilocus. O DNA de N. caninum não foi detectado por PCR. As 2 amostras genotipadas eram do tipo III para todos os marcadores, exceto para SAG-1 (tipo I para Rat1Arg e tipo II / III para Rat2Arg) e foram identificadas como # 48 e # 2 (provavelmente) de acordo com as combinações de alelos relatadas no Toxo DB (Toxo-DB). Os resultados do presente estudo indicam uma ampla distribuição de T. gondii e menor para N. caninum, em ratos e camundongos sinantrópicos na área estudada.
Facultad de Ciencias Veterinarias
Centro de Estudios Parasitológicos y de Vectores - Materia
-
Ciencias Veterinarias
Toxoplasma gondii
Neospora caninum
Synanthropic rodent
Serology
Genotyping - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/123713
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<i>Toxoplasma gondii</i> and <i>Neospora caninum</i> infections in synanthropic rodents from ArgentinaInfecções por Toxoplasma gondii e Neospora caninum em roedores sinantrópicos da ArgentinaDellarupe, AndreaFitte, BrunoPardini, LaisCampero, Lucía MaríaBernstein, MarianaRobles, María del RosarioMoré, Gastón AndrésVenturini, María CeciliaUnzaga, Juan ManuelCiencias VeterinariasToxoplasma gondiiNeospora caninumSynanthropic rodentSerologyGenotypingToxoplasma gondii and Neospora caninum are closely related coccidian parasites (phylum Apicomplexa). This is the first study from urban synanthropic rodent species that involved serological and molecular diagnosis of T. gondii and N. caninum infection, and genotyping of T. gondii in Argentina. A total of 127 rodent samples were trap captured: Mus musculus (n = 78), Rattus norvegicus (n = 26) and Rattus rattus (n = 23). Antibodies against T. gondii and N. caninum were detected by IFAT in 32.8% (40/122) and 0.8% (1/122) of rodent samples, respectively, demonstrating contact with these protozoans. Additionally, T. gondii DNA was detected in 3.3% (4/123) of rodent central nervous system samples and 2 samples were genotyped by multilocus nPCR-RFLP. Neospora caninum DNA was not detected by PCR. The 2 genotyped samples were type III allele for all markers except for SAG-1 (type I for Rat1Arg and type II/III for Rat2Arg) and were identified as #48 and #2 (likely) according to the allele combinations reported on Toxo DB (Toxo-DB). The results of the present study revealed a wide distribution of T. gondii and less for N. caninum, in synanthropic rats and mice in the studied area.Toxoplasma gondii e Neospora caninum são parasitas coccídeos intimamente relacionados (filo Apicomplexa). Este é o primeiro estudo de espécies de roedores sinantrópicos urbanos, o qual envolveu diagnósticos sorológicos e moleculares da infecção por T. gondii e N. caninum e genotipagem de T. gondii na Argentina. Um total de 127 amostras de roedores foram obtidas: Mus musculus (n = 78), Rattus norvegicus (n = 26) e Rattus rattus (n = 23). Anticorpos contra T. gondii e N. caninum foram detectados pela IFAT em 32,8% (40/122) e 0,8% (1/122) das amostras de roedores, respectivamente, demonstrando contato com esses protozoários. Adicionalmente, o DNA de T. gondii foi detectado em 3,3% (4/123) das amostras do sistema nervoso central de roedores e duas amostras foram genotipadas por nPCR-RFLP multilocus. O DNA de N. caninum não foi detectado por PCR. As 2 amostras genotipadas eram do tipo III para todos os marcadores, exceto para SAG-1 (tipo I para Rat1Arg e tipo II / III para Rat2Arg) e foram identificadas como # 48 e # 2 (provavelmente) de acordo com as combinações de alelos relatadas no Toxo DB (Toxo-DB). Os resultados do presente estudo indicam uma ampla distribuição de T. gondii e menor para N. caninum, em ratos e camundongos sinantrópicos na área estudada.Facultad de Ciencias VeterinariasCentro de Estudios Parasitológicos y de Vectores2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf113-118http://sedici.unlp.edu.ar/handle/10915/123713enginfo:eu-repo/semantics/altIdentifier/issn/1984-2961info:eu-repo/semantics/altIdentifier/issn/0103-846Xinfo:eu-repo/semantics/altIdentifier/pmid/30916257info:eu-repo/semantics/altIdentifier/doi/10.1590/s1984-29612019009info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T11:01:33Zoai:sedici.unlp.edu.ar:10915/123713Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 11:01:34.154SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
<i>Toxoplasma gondii</i> and <i>Neospora caninum</i> infections in synanthropic rodents from Argentina Infecções por Toxoplasma gondii e Neospora caninum em roedores sinantrópicos da Argentina |
title |
<i>Toxoplasma gondii</i> and <i>Neospora caninum</i> infections in synanthropic rodents from Argentina |
spellingShingle |
<i>Toxoplasma gondii</i> and <i>Neospora caninum</i> infections in synanthropic rodents from Argentina Dellarupe, Andrea Ciencias Veterinarias Toxoplasma gondii Neospora caninum Synanthropic rodent Serology Genotyping |
title_short |
<i>Toxoplasma gondii</i> and <i>Neospora caninum</i> infections in synanthropic rodents from Argentina |
title_full |
<i>Toxoplasma gondii</i> and <i>Neospora caninum</i> infections in synanthropic rodents from Argentina |
title_fullStr |
<i>Toxoplasma gondii</i> and <i>Neospora caninum</i> infections in synanthropic rodents from Argentina |
title_full_unstemmed |
<i>Toxoplasma gondii</i> and <i>Neospora caninum</i> infections in synanthropic rodents from Argentina |
title_sort |
<i>Toxoplasma gondii</i> and <i>Neospora caninum</i> infections in synanthropic rodents from Argentina |
dc.creator.none.fl_str_mv |
Dellarupe, Andrea Fitte, Bruno Pardini, Lais Campero, Lucía María Bernstein, Mariana Robles, María del Rosario Moré, Gastón Andrés Venturini, María Cecilia Unzaga, Juan Manuel |
author |
Dellarupe, Andrea |
author_facet |
Dellarupe, Andrea Fitte, Bruno Pardini, Lais Campero, Lucía María Bernstein, Mariana Robles, María del Rosario Moré, Gastón Andrés Venturini, María Cecilia Unzaga, Juan Manuel |
author_role |
author |
author2 |
Fitte, Bruno Pardini, Lais Campero, Lucía María Bernstein, Mariana Robles, María del Rosario Moré, Gastón Andrés Venturini, María Cecilia Unzaga, Juan Manuel |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
Ciencias Veterinarias Toxoplasma gondii Neospora caninum Synanthropic rodent Serology Genotyping |
topic |
Ciencias Veterinarias Toxoplasma gondii Neospora caninum Synanthropic rodent Serology Genotyping |
dc.description.none.fl_txt_mv |
Toxoplasma gondii and Neospora caninum are closely related coccidian parasites (phylum Apicomplexa). This is the first study from urban synanthropic rodent species that involved serological and molecular diagnosis of T. gondii and N. caninum infection, and genotyping of T. gondii in Argentina. A total of 127 rodent samples were trap captured: Mus musculus (n = 78), Rattus norvegicus (n = 26) and Rattus rattus (n = 23). Antibodies against T. gondii and N. caninum were detected by IFAT in 32.8% (40/122) and 0.8% (1/122) of rodent samples, respectively, demonstrating contact with these protozoans. Additionally, T. gondii DNA was detected in 3.3% (4/123) of rodent central nervous system samples and 2 samples were genotyped by multilocus nPCR-RFLP. Neospora caninum DNA was not detected by PCR. The 2 genotyped samples were type III allele for all markers except for SAG-1 (type I for Rat1Arg and type II/III for Rat2Arg) and were identified as #48 and #2 (likely) according to the allele combinations reported on Toxo DB (Toxo-DB). The results of the present study revealed a wide distribution of T. gondii and less for N. caninum, in synanthropic rats and mice in the studied area. Toxoplasma gondii e Neospora caninum são parasitas coccídeos intimamente relacionados (filo Apicomplexa). Este é o primeiro estudo de espécies de roedores sinantrópicos urbanos, o qual envolveu diagnósticos sorológicos e moleculares da infecção por T. gondii e N. caninum e genotipagem de T. gondii na Argentina. Um total de 127 amostras de roedores foram obtidas: Mus musculus (n = 78), Rattus norvegicus (n = 26) e Rattus rattus (n = 23). Anticorpos contra T. gondii e N. caninum foram detectados pela IFAT em 32,8% (40/122) e 0,8% (1/122) das amostras de roedores, respectivamente, demonstrando contato com esses protozoários. Adicionalmente, o DNA de T. gondii foi detectado em 3,3% (4/123) das amostras do sistema nervoso central de roedores e duas amostras foram genotipadas por nPCR-RFLP multilocus. O DNA de N. caninum não foi detectado por PCR. As 2 amostras genotipadas eram do tipo III para todos os marcadores, exceto para SAG-1 (tipo I para Rat1Arg e tipo II / III para Rat2Arg) e foram identificadas como # 48 e # 2 (provavelmente) de acordo com as combinações de alelos relatadas no Toxo DB (Toxo-DB). Os resultados do presente estudo indicam uma ampla distribuição de T. gondii e menor para N. caninum, em ratos e camundongos sinantrópicos na área estudada. Facultad de Ciencias Veterinarias Centro de Estudios Parasitológicos y de Vectores |
description |
Toxoplasma gondii and Neospora caninum are closely related coccidian parasites (phylum Apicomplexa). This is the first study from urban synanthropic rodent species that involved serological and molecular diagnosis of T. gondii and N. caninum infection, and genotyping of T. gondii in Argentina. A total of 127 rodent samples were trap captured: Mus musculus (n = 78), Rattus norvegicus (n = 26) and Rattus rattus (n = 23). Antibodies against T. gondii and N. caninum were detected by IFAT in 32.8% (40/122) and 0.8% (1/122) of rodent samples, respectively, demonstrating contact with these protozoans. Additionally, T. gondii DNA was detected in 3.3% (4/123) of rodent central nervous system samples and 2 samples were genotyped by multilocus nPCR-RFLP. Neospora caninum DNA was not detected by PCR. The 2 genotyped samples were type III allele for all markers except for SAG-1 (type I for Rat1Arg and type II/III for Rat2Arg) and were identified as #48 and #2 (likely) according to the allele combinations reported on Toxo DB (Toxo-DB). The results of the present study revealed a wide distribution of T. gondii and less for N. caninum, in synanthropic rats and mice in the studied area. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019 |
dc.type.none.fl_str_mv |
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format |
article |
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publishedVersion |
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eng |
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eng |
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info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
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openAccess |
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