Cell wall variability in the green seaweed Codium vermilara (Bryopsidales Chlorophyta) from Argentine coast

Autores
Fernández, Paula Virginia; Ciancia, Marina; Estevez, Jose Manuel
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cell wall chemistry in the coencocytic green seaweed Codium vermilara (Olivi) Chiaje (Bryopsidales, Chlorophyta) is well understood. These cell walls are composed of major amounts of neutral beta 1,4-D-mannans (Mn), sulfated polysaccharides (SPs), which include pyranosic arabinan sulfates (ArpS), pyruvylated galactan sulfates (pGaS), and mannan sulfates (MnS); also minor amounts of O-glycoproteins are present. In this study, cell wall samples of C. vermilara were investigated with regard to their monosaccharide composition and infrared spectra (by Fourier Transformed Infrared spectroscopy coupled to Principal Component (FTIR-PC) analysis). Samples from three different populations of C. vermilara from Argentine coast showed: 1) an important variation in the relative arabinan content, which increases from north to south, and 2) a measurable degree of cell wall variability in the sulfate distribution between the different sulfated polysaccharides, independently of the amount of each polysaccharide present and of total sulfate content. When cell wall composition was analyzed over three consecutive years in a single geographical location, the quantity of Mn and overall sulfate content on SPs remained constant, whereas pGaS:ArpS molar ratio changed over the time. Besides, similar cell wall composition was found between actively-growing and resting zones of the thallus, suggesting that cell wall composition is independent of growth stage and development. Overall, these results suggest that C. vermilara has developed a mechanism to adjust the total level of cell wall sulfation by modulating ArpS:pGaS:MnS molar ratio and also by adjusting the of sulfation level in each type of polymer, whereas non-sulfated Mn, as the main structural polysaccharide, does not change over the time or growing stage.
Fil: Fernández, Paula Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Ciancia, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Estevez, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Materia
cell wall
temporal and geographical variation
mannans
sulfated polysaccharides
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/278205
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/278205
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cell wall variability in the green seaweed Codium vermilara (Bryopsidales Chlorophyta) from Argentine coastFernández, Paula VirginiaCiancia, MarinaEstevez, Jose Manuelcell walltemporal and geographical variationmannanssulfated polysaccharideshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Cell wall chemistry in the coencocytic green seaweed Codium vermilara (Olivi) Chiaje (Bryopsidales, Chlorophyta) is well understood. These cell walls are composed of major amounts of neutral beta 1,4-D-mannans (Mn), sulfated polysaccharides (SPs), which include pyranosic arabinan sulfates (ArpS), pyruvylated galactan sulfates (pGaS), and mannan sulfates (MnS); also minor amounts of O-glycoproteins are present. In this study, cell wall samples of C. vermilara were investigated with regard to their monosaccharide composition and infrared spectra (by Fourier Transformed Infrared spectroscopy coupled to Principal Component (FTIR-PC) analysis). Samples from three different populations of C. vermilara from Argentine coast showed: 1) an important variation in the relative arabinan content, which increases from north to south, and 2) a measurable degree of cell wall variability in the sulfate distribution between the different sulfated polysaccharides, independently of the amount of each polysaccharide present and of total sulfate content. When cell wall composition was analyzed over three consecutive years in a single geographical location, the quantity of Mn and overall sulfate content on SPs remained constant, whereas pGaS:ArpS molar ratio changed over the time. Besides, similar cell wall composition was found between actively-growing and resting zones of the thallus, suggesting that cell wall composition is independent of growth stage and development. Overall, these results suggest that C. vermilara has developed a mechanism to adjust the total level of cell wall sulfation by modulating ArpS:pGaS:MnS molar ratio and also by adjusting the of sulfation level in each type of polymer, whereas non-sulfated Mn, as the main structural polysaccharide, does not change over the time or growing stage.Fil: Fernández, Paula Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Ciancia, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Estevez, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaWiley Blackwell Publishing, Inc2011-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/278205Fernández, Paula Virginia; Ciancia, Marina; Estevez, Jose Manuel; Cell wall variability in the green seaweed Codium vermilara (Bryopsidales Chlorophyta) from Argentine coast; Wiley Blackwell Publishing, Inc; Journal of Phycology; 47; 4; 8-2011; 802-8100022-3646CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1529-8817.2011.01006.x/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1529-8817.2011.01006.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-23T13:18:45Zoai:ri.conicet.gov.ar:11336/278205instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-23 13:18:45.404CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cell wall variability in the green seaweed Codium vermilara (Bryopsidales Chlorophyta) from Argentine coast
title Cell wall variability in the green seaweed Codium vermilara (Bryopsidales Chlorophyta) from Argentine coast
spellingShingle Cell wall variability in the green seaweed Codium vermilara (Bryopsidales Chlorophyta) from Argentine coast
Fernández, Paula Virginia
cell wall
temporal and geographical variation
mannans
sulfated polysaccharides
title_short Cell wall variability in the green seaweed Codium vermilara (Bryopsidales Chlorophyta) from Argentine coast
title_full Cell wall variability in the green seaweed Codium vermilara (Bryopsidales Chlorophyta) from Argentine coast
title_fullStr Cell wall variability in the green seaweed Codium vermilara (Bryopsidales Chlorophyta) from Argentine coast
title_full_unstemmed Cell wall variability in the green seaweed Codium vermilara (Bryopsidales Chlorophyta) from Argentine coast
title_sort Cell wall variability in the green seaweed Codium vermilara (Bryopsidales Chlorophyta) from Argentine coast
dc.creator.none.fl_str_mv Fernández, Paula Virginia
Ciancia, Marina
Estevez, Jose Manuel
author Fernández, Paula Virginia
author_facet Fernández, Paula Virginia
Ciancia, Marina
Estevez, Jose Manuel
author_role author
author2 Ciancia, Marina
Estevez, Jose Manuel
author2_role author
author
dc.subject.none.fl_str_mv cell wall
temporal and geographical variation
mannans
sulfated polysaccharides
topic cell wall
temporal and geographical variation
mannans
sulfated polysaccharides
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Cell wall chemistry in the coencocytic green seaweed Codium vermilara (Olivi) Chiaje (Bryopsidales, Chlorophyta) is well understood. These cell walls are composed of major amounts of neutral beta 1,4-D-mannans (Mn), sulfated polysaccharides (SPs), which include pyranosic arabinan sulfates (ArpS), pyruvylated galactan sulfates (pGaS), and mannan sulfates (MnS); also minor amounts of O-glycoproteins are present. In this study, cell wall samples of C. vermilara were investigated with regard to their monosaccharide composition and infrared spectra (by Fourier Transformed Infrared spectroscopy coupled to Principal Component (FTIR-PC) analysis). Samples from three different populations of C. vermilara from Argentine coast showed: 1) an important variation in the relative arabinan content, which increases from north to south, and 2) a measurable degree of cell wall variability in the sulfate distribution between the different sulfated polysaccharides, independently of the amount of each polysaccharide present and of total sulfate content. When cell wall composition was analyzed over three consecutive years in a single geographical location, the quantity of Mn and overall sulfate content on SPs remained constant, whereas pGaS:ArpS molar ratio changed over the time. Besides, similar cell wall composition was found between actively-growing and resting zones of the thallus, suggesting that cell wall composition is independent of growth stage and development. Overall, these results suggest that C. vermilara has developed a mechanism to adjust the total level of cell wall sulfation by modulating ArpS:pGaS:MnS molar ratio and also by adjusting the of sulfation level in each type of polymer, whereas non-sulfated Mn, as the main structural polysaccharide, does not change over the time or growing stage.
Fil: Fernández, Paula Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Ciancia, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Estevez, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
description Cell wall chemistry in the coencocytic green seaweed Codium vermilara (Olivi) Chiaje (Bryopsidales, Chlorophyta) is well understood. These cell walls are composed of major amounts of neutral beta 1,4-D-mannans (Mn), sulfated polysaccharides (SPs), which include pyranosic arabinan sulfates (ArpS), pyruvylated galactan sulfates (pGaS), and mannan sulfates (MnS); also minor amounts of O-glycoproteins are present. In this study, cell wall samples of C. vermilara were investigated with regard to their monosaccharide composition and infrared spectra (by Fourier Transformed Infrared spectroscopy coupled to Principal Component (FTIR-PC) analysis). Samples from three different populations of C. vermilara from Argentine coast showed: 1) an important variation in the relative arabinan content, which increases from north to south, and 2) a measurable degree of cell wall variability in the sulfate distribution between the different sulfated polysaccharides, independently of the amount of each polysaccharide present and of total sulfate content. When cell wall composition was analyzed over three consecutive years in a single geographical location, the quantity of Mn and overall sulfate content on SPs remained constant, whereas pGaS:ArpS molar ratio changed over the time. Besides, similar cell wall composition was found between actively-growing and resting zones of the thallus, suggesting that cell wall composition is independent of growth stage and development. Overall, these results suggest that C. vermilara has developed a mechanism to adjust the total level of cell wall sulfation by modulating ArpS:pGaS:MnS molar ratio and also by adjusting the of sulfation level in each type of polymer, whereas non-sulfated Mn, as the main structural polysaccharide, does not change over the time or growing stage.
publishDate 2011
dc.date.none.fl_str_mv 2011-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/278205
Fernández, Paula Virginia; Ciancia, Marina; Estevez, Jose Manuel; Cell wall variability in the green seaweed Codium vermilara (Bryopsidales Chlorophyta) from Argentine coast; Wiley Blackwell Publishing, Inc; Journal of Phycology; 47; 4; 8-2011; 802-810
0022-3646
CONICET Digital
CONICET
url http://hdl.handle.net/11336/278205
identifier_str_mv Fernández, Paula Virginia; Ciancia, Marina; Estevez, Jose Manuel; Cell wall variability in the green seaweed Codium vermilara (Bryopsidales Chlorophyta) from Argentine coast; Wiley Blackwell Publishing, Inc; Journal of Phycology; 47; 4; 8-2011; 802-810
0022-3646
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1529-8817.2011.01006.x/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1529-8817.2011.01006.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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