Chemical structure and anticoagulant activity of highly pyruvylated sulfated galactans from tropical green seaweeds of the order Bryopsidales

Autores
Arata, Paula Ximena; Quintana, Irene Luisa; Canelon Gonzalez, Dilsia Josefina; Vera, Beatriz E.; Compagnone, Reinaldo S.; Ciancia, Marina
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Sulfated and pyruvylated galactans were isolated from three tropical species of the Bryopsidales, Penicillus capitatus, Udotea flabellum, and Halimeda opuntia. They represent the only important sulfated polysaccharides present in the cell walls of these highly calcified seaweeds of the suborder Halimedineae. Their structural features were studied by chemical analyses and NMR spectroscopy. Their backbone comprises 3-, 6-, and 3,6-linkages, constituted by major amounts of 3-linked 4,6-O-(1′-carboxy)ethylidene-d-galactopyranose units in part sulfated on C-2. Sulfation on C-2 was not found in galactans from other seaweeds of this order. In addition, a complex sulfation pattern, comprising also 4-, 6-, and 4,6-disulfated galactose units was found. A fraction from P. capitatus, F1, showed a moderate anticoagulant activity, evaluated by general coagulation tests and also kinetics of fibrin formation was assayed. Besides, preliminary results suggest that one of the possible mechanisms involved is direct thrombin inhibition.
Fil: Arata, Paula Ximena. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Biología Aplicada y Alimentos; Argentina
Fil: Quintana, Irene Luisa. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Canelon Gonzalez, Dilsia Josefina. Universidad Central de Venezuela; Venezuela
Fil: Vera, Beatriz E.. Universidad Central de Venezuela; Venezuela
Fil: Compagnone, Reinaldo S.. Universidad Central de Venezuela; Venezuela
Fil: Ciancia, Marina. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Biología Aplicada y Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Materia
Sulfated Polysaccharide
Green Seaweed
Bryopsidales
Structure of Polysaccharides
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/17787
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/17787
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Chemical structure and anticoagulant activity of highly pyruvylated sulfated galactans from tropical green seaweeds of the order BryopsidalesArata, Paula XimenaQuintana, Irene LuisaCanelon Gonzalez, Dilsia JosefinaVera, Beatriz E.Compagnone, Reinaldo S.Ciancia, MarinaSulfated PolysaccharideGreen SeaweedBryopsidalesStructure of Polysaccharideshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Sulfated and pyruvylated galactans were isolated from three tropical species of the Bryopsidales, Penicillus capitatus, Udotea flabellum, and Halimeda opuntia. They represent the only important sulfated polysaccharides present in the cell walls of these highly calcified seaweeds of the suborder Halimedineae. Their structural features were studied by chemical analyses and NMR spectroscopy. Their backbone comprises 3-, 6-, and 3,6-linkages, constituted by major amounts of 3-linked 4,6-O-(1′-carboxy)ethylidene-d-galactopyranose units in part sulfated on C-2. Sulfation on C-2 was not found in galactans from other seaweeds of this order. In addition, a complex sulfation pattern, comprising also 4-, 6-, and 4,6-disulfated galactose units was found. A fraction from P. capitatus, F1, showed a moderate anticoagulant activity, evaluated by general coagulation tests and also kinetics of fibrin formation was assayed. Besides, preliminary results suggest that one of the possible mechanisms involved is direct thrombin inhibition.Fil: Arata, Paula Ximena. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Biología Aplicada y Alimentos; ArgentinaFil: Quintana, Irene Luisa. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Canelon Gonzalez, Dilsia Josefina. Universidad Central de Venezuela; VenezuelaFil: Vera, Beatriz E.. Universidad Central de Venezuela; VenezuelaFil: Compagnone, Reinaldo S.. Universidad Central de Venezuela; VenezuelaFil: Ciancia, Marina. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Biología Aplicada y Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaElsevier2015-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/17787Arata, Paula Ximena; Quintana, Irene Luisa; Canelon Gonzalez, Dilsia Josefina; Vera, Beatriz E.; Compagnone, Reinaldo S.; et al.; Chemical structure and anticoagulant activity of highly pyruvylated sulfated galactans from tropical green seaweeds of the order Bryopsidales; Elsevier; Carbohydrate Polymers; 122; 5-2015; 376-3860144-8617enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.carbpol.2014.10.030info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0144861714010352info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:54:20Zoai:ri.conicet.gov.ar:11336/17787instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:54:20.413CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Chemical structure and anticoagulant activity of highly pyruvylated sulfated galactans from tropical green seaweeds of the order Bryopsidales
title Chemical structure and anticoagulant activity of highly pyruvylated sulfated galactans from tropical green seaweeds of the order Bryopsidales
spellingShingle Chemical structure and anticoagulant activity of highly pyruvylated sulfated galactans from tropical green seaweeds of the order Bryopsidales
Arata, Paula Ximena
Sulfated Polysaccharide
Green Seaweed
Bryopsidales
Structure of Polysaccharides
title_short Chemical structure and anticoagulant activity of highly pyruvylated sulfated galactans from tropical green seaweeds of the order Bryopsidales
title_full Chemical structure and anticoagulant activity of highly pyruvylated sulfated galactans from tropical green seaweeds of the order Bryopsidales
title_fullStr Chemical structure and anticoagulant activity of highly pyruvylated sulfated galactans from tropical green seaweeds of the order Bryopsidales
title_full_unstemmed Chemical structure and anticoagulant activity of highly pyruvylated sulfated galactans from tropical green seaweeds of the order Bryopsidales
title_sort Chemical structure and anticoagulant activity of highly pyruvylated sulfated galactans from tropical green seaweeds of the order Bryopsidales
dc.creator.none.fl_str_mv Arata, Paula Ximena
Quintana, Irene Luisa
Canelon Gonzalez, Dilsia Josefina
Vera, Beatriz E.
Compagnone, Reinaldo S.
Ciancia, Marina
author Arata, Paula Ximena
author_facet Arata, Paula Ximena
Quintana, Irene Luisa
Canelon Gonzalez, Dilsia Josefina
Vera, Beatriz E.
Compagnone, Reinaldo S.
Ciancia, Marina
author_role author
author2 Quintana, Irene Luisa
Canelon Gonzalez, Dilsia Josefina
Vera, Beatriz E.
Compagnone, Reinaldo S.
Ciancia, Marina
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Sulfated Polysaccharide
Green Seaweed
Bryopsidales
Structure of Polysaccharides
topic Sulfated Polysaccharide
Green Seaweed
Bryopsidales
Structure of Polysaccharides
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Sulfated and pyruvylated galactans were isolated from three tropical species of the Bryopsidales, Penicillus capitatus, Udotea flabellum, and Halimeda opuntia. They represent the only important sulfated polysaccharides present in the cell walls of these highly calcified seaweeds of the suborder Halimedineae. Their structural features were studied by chemical analyses and NMR spectroscopy. Their backbone comprises 3-, 6-, and 3,6-linkages, constituted by major amounts of 3-linked 4,6-O-(1′-carboxy)ethylidene-d-galactopyranose units in part sulfated on C-2. Sulfation on C-2 was not found in galactans from other seaweeds of this order. In addition, a complex sulfation pattern, comprising also 4-, 6-, and 4,6-disulfated galactose units was found. A fraction from P. capitatus, F1, showed a moderate anticoagulant activity, evaluated by general coagulation tests and also kinetics of fibrin formation was assayed. Besides, preliminary results suggest that one of the possible mechanisms involved is direct thrombin inhibition.
Fil: Arata, Paula Ximena. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Biología Aplicada y Alimentos; Argentina
Fil: Quintana, Irene Luisa. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Canelon Gonzalez, Dilsia Josefina. Universidad Central de Venezuela; Venezuela
Fil: Vera, Beatriz E.. Universidad Central de Venezuela; Venezuela
Fil: Compagnone, Reinaldo S.. Universidad Central de Venezuela; Venezuela
Fil: Ciancia, Marina. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Biología Aplicada y Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
description Sulfated and pyruvylated galactans were isolated from three tropical species of the Bryopsidales, Penicillus capitatus, Udotea flabellum, and Halimeda opuntia. They represent the only important sulfated polysaccharides present in the cell walls of these highly calcified seaweeds of the suborder Halimedineae. Their structural features were studied by chemical analyses and NMR spectroscopy. Their backbone comprises 3-, 6-, and 3,6-linkages, constituted by major amounts of 3-linked 4,6-O-(1′-carboxy)ethylidene-d-galactopyranose units in part sulfated on C-2. Sulfation on C-2 was not found in galactans from other seaweeds of this order. In addition, a complex sulfation pattern, comprising also 4-, 6-, and 4,6-disulfated galactose units was found. A fraction from P. capitatus, F1, showed a moderate anticoagulant activity, evaluated by general coagulation tests and also kinetics of fibrin formation was assayed. Besides, preliminary results suggest that one of the possible mechanisms involved is direct thrombin inhibition.
publishDate 2015
dc.date.none.fl_str_mv 2015-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/17787
Arata, Paula Ximena; Quintana, Irene Luisa; Canelon Gonzalez, Dilsia Josefina; Vera, Beatriz E.; Compagnone, Reinaldo S.; et al.; Chemical structure and anticoagulant activity of highly pyruvylated sulfated galactans from tropical green seaweeds of the order Bryopsidales; Elsevier; Carbohydrate Polymers; 122; 5-2015; 376-386
0144-8617
url http://hdl.handle.net/11336/17787
identifier_str_mv Arata, Paula Ximena; Quintana, Irene Luisa; Canelon Gonzalez, Dilsia Josefina; Vera, Beatriz E.; Compagnone, Reinaldo S.; et al.; Chemical structure and anticoagulant activity of highly pyruvylated sulfated galactans from tropical green seaweeds of the order Bryopsidales; Elsevier; Carbohydrate Polymers; 122; 5-2015; 376-386
0144-8617
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.carbpol.2014.10.030
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0144861714010352
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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