Cell wall polymer mapping in the coenocytic seaweed Codium vermilara (Bryopsidales, Chlorophyta)

Autores
Fernández, Paula Virginia; Ciancia, Marina; Miravalles, Alicia Beatriz; Estevez, Jose Manuel
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cell walls in the coenocytic green seaweed Codium vermilara (Olivi) Chiaje (Bryopsidales, Chlorophyta) are composed of ∼32% (w/w) β-(1→4)-d-mannans, ∼12% sulfated polysaccharides (SPs), and small amounts of hydroxyproline-rich glycoprotein-like (HRGP-L) compounds of the arabinogalactan proteins (AGPs) and arabinosides (extensins). Similar quantities of mannans and SPs were reported previously in the related seaweed C. fragile (Suringar) Hariot. Overall, both seaweed cell walls comprise ∼40%-44% of their dry weights. Within the SP group, a variety of polysaccharide structures from pyruvylated arabinogalactan sulfate and pyruvylated galactan sulfate to pyranosic arabinan sulfate are present in Codium cell walls. In this paper, the in situ distribution of the main cell-wall polymers in the green seaweed C. vermilara was studied, comparing their arrangements with those observed in cell walls from C. fragile. The utricle cell wall in C. vermilara showed by TEM a sandwich structure of two fibrillar-like layers of similar width delimiting a middle amorphous-like zone. By immuno- and chemical imaging, the in situ distribution of β-(1→4)-d-mannans and HRGP-like epitopes was shown to consist of two distinct cell-wall layers, whereas SPs are distributed in the middle area of the wall. The overall cell-wall polymer arrangement of the SPs, HRGP-like epitopes, and mannans in the utricles of C. vermilara is different from the ubiquitous green algae C. fragile, in spite of both being phylogenetically very close. In addition, a preliminary cell-wall model of the utricle moiety is proposed for both seaweeds, C. fragile and C.
Fil: Fernández, Paula Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Ciancia, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Miravalles, Alicia Beatriz. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Estevez, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Materia
Β-(1→4)-D-MANNANS
CARBOHYDRATE IMMUNOLABELING
CELL-WALL ARRANGEMENT
CHEMICAL IMAGING
CODIUM VERMILARA
COENOCYTIC GREEN SEAWEED
HYDROXYPROLINE-RICH GLYCOPROTEIN-LIKE
SR-FTIR MICROSPECTROSCOPY
SULFATED POLYSACCHARIDES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/277288
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/277288
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cell wall polymer mapping in the coenocytic seaweed Codium vermilara (Bryopsidales, Chlorophyta)Fernández, Paula VirginiaCiancia, MarinaMiravalles, Alicia BeatrizEstevez, Jose ManuelΒ-(1→4)-D-MANNANSCARBOHYDRATE IMMUNOLABELINGCELL-WALL ARRANGEMENTCHEMICAL IMAGINGCODIUM VERMILARACOENOCYTIC GREEN SEAWEEDHYDROXYPROLINE-RICH GLYCOPROTEIN-LIKESR-FTIR MICROSPECTROSCOPYSULFATED POLYSACCHARIDEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Cell walls in the coenocytic green seaweed Codium vermilara (Olivi) Chiaje (Bryopsidales, Chlorophyta) are composed of ∼32% (w/w) β-(1→4)-d-mannans, ∼12% sulfated polysaccharides (SPs), and small amounts of hydroxyproline-rich glycoprotein-like (HRGP-L) compounds of the arabinogalactan proteins (AGPs) and arabinosides (extensins). Similar quantities of mannans and SPs were reported previously in the related seaweed C. fragile (Suringar) Hariot. Overall, both seaweed cell walls comprise ∼40%-44% of their dry weights. Within the SP group, a variety of polysaccharide structures from pyruvylated arabinogalactan sulfate and pyruvylated galactan sulfate to pyranosic arabinan sulfate are present in Codium cell walls. In this paper, the in situ distribution of the main cell-wall polymers in the green seaweed C. vermilara was studied, comparing their arrangements with those observed in cell walls from C. fragile. The utricle cell wall in C. vermilara showed by TEM a sandwich structure of two fibrillar-like layers of similar width delimiting a middle amorphous-like zone. By immuno- and chemical imaging, the in situ distribution of β-(1→4)-d-mannans and HRGP-like epitopes was shown to consist of two distinct cell-wall layers, whereas SPs are distributed in the middle area of the wall. The overall cell-wall polymer arrangement of the SPs, HRGP-like epitopes, and mannans in the utricles of C. vermilara is different from the ubiquitous green algae C. fragile, in spite of both being phylogenetically very close. In addition, a preliminary cell-wall model of the utricle moiety is proposed for both seaweeds, C. fragile and C.Fil: Fernández, Paula Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Ciancia, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Miravalles, Alicia Beatriz. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Estevez, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaWiley Blackwell Publishing, Inc2010-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/277288Fernández, Paula Virginia; Ciancia, Marina; Miravalles, Alicia Beatriz; Estevez, Jose Manuel; Cell wall polymer mapping in the coenocytic seaweed Codium vermilara (Bryopsidales, Chlorophyta); Wiley Blackwell Publishing, Inc; Journal of Phycology; 46; 3; 6-2010; 456-4650022-3646CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/j.1529-8817.2010.00821.xinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1529-8817.2010.00821.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-23T13:15:52Zoai:ri.conicet.gov.ar:11336/277288instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-23 13:15:52.335CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cell wall polymer mapping in the coenocytic seaweed Codium vermilara (Bryopsidales, Chlorophyta)
title Cell wall polymer mapping in the coenocytic seaweed Codium vermilara (Bryopsidales, Chlorophyta)
spellingShingle Cell wall polymer mapping in the coenocytic seaweed Codium vermilara (Bryopsidales, Chlorophyta)
Fernández, Paula Virginia
Β-(1→4)-D-MANNANS
CARBOHYDRATE IMMUNOLABELING
CELL-WALL ARRANGEMENT
CHEMICAL IMAGING
CODIUM VERMILARA
COENOCYTIC GREEN SEAWEED
HYDROXYPROLINE-RICH GLYCOPROTEIN-LIKE
SR-FTIR MICROSPECTROSCOPY
SULFATED POLYSACCHARIDES
title_short Cell wall polymer mapping in the coenocytic seaweed Codium vermilara (Bryopsidales, Chlorophyta)
title_full Cell wall polymer mapping in the coenocytic seaweed Codium vermilara (Bryopsidales, Chlorophyta)
title_fullStr Cell wall polymer mapping in the coenocytic seaweed Codium vermilara (Bryopsidales, Chlorophyta)
title_full_unstemmed Cell wall polymer mapping in the coenocytic seaweed Codium vermilara (Bryopsidales, Chlorophyta)
title_sort Cell wall polymer mapping in the coenocytic seaweed Codium vermilara (Bryopsidales, Chlorophyta)
dc.creator.none.fl_str_mv Fernández, Paula Virginia
Ciancia, Marina
Miravalles, Alicia Beatriz
Estevez, Jose Manuel
author Fernández, Paula Virginia
author_facet Fernández, Paula Virginia
Ciancia, Marina
Miravalles, Alicia Beatriz
Estevez, Jose Manuel
author_role author
author2 Ciancia, Marina
Miravalles, Alicia Beatriz
Estevez, Jose Manuel
author2_role author
author
author
dc.subject.none.fl_str_mv Β-(1→4)-D-MANNANS
CARBOHYDRATE IMMUNOLABELING
CELL-WALL ARRANGEMENT
CHEMICAL IMAGING
CODIUM VERMILARA
COENOCYTIC GREEN SEAWEED
HYDROXYPROLINE-RICH GLYCOPROTEIN-LIKE
SR-FTIR MICROSPECTROSCOPY
SULFATED POLYSACCHARIDES
topic Β-(1→4)-D-MANNANS
CARBOHYDRATE IMMUNOLABELING
CELL-WALL ARRANGEMENT
CHEMICAL IMAGING
CODIUM VERMILARA
COENOCYTIC GREEN SEAWEED
HYDROXYPROLINE-RICH GLYCOPROTEIN-LIKE
SR-FTIR MICROSPECTROSCOPY
SULFATED POLYSACCHARIDES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Cell walls in the coenocytic green seaweed Codium vermilara (Olivi) Chiaje (Bryopsidales, Chlorophyta) are composed of ∼32% (w/w) β-(1→4)-d-mannans, ∼12% sulfated polysaccharides (SPs), and small amounts of hydroxyproline-rich glycoprotein-like (HRGP-L) compounds of the arabinogalactan proteins (AGPs) and arabinosides (extensins). Similar quantities of mannans and SPs were reported previously in the related seaweed C. fragile (Suringar) Hariot. Overall, both seaweed cell walls comprise ∼40%-44% of their dry weights. Within the SP group, a variety of polysaccharide structures from pyruvylated arabinogalactan sulfate and pyruvylated galactan sulfate to pyranosic arabinan sulfate are present in Codium cell walls. In this paper, the in situ distribution of the main cell-wall polymers in the green seaweed C. vermilara was studied, comparing their arrangements with those observed in cell walls from C. fragile. The utricle cell wall in C. vermilara showed by TEM a sandwich structure of two fibrillar-like layers of similar width delimiting a middle amorphous-like zone. By immuno- and chemical imaging, the in situ distribution of β-(1→4)-d-mannans and HRGP-like epitopes was shown to consist of two distinct cell-wall layers, whereas SPs are distributed in the middle area of the wall. The overall cell-wall polymer arrangement of the SPs, HRGP-like epitopes, and mannans in the utricles of C. vermilara is different from the ubiquitous green algae C. fragile, in spite of both being phylogenetically very close. In addition, a preliminary cell-wall model of the utricle moiety is proposed for both seaweeds, C. fragile and C.
Fil: Fernández, Paula Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Ciancia, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Miravalles, Alicia Beatriz. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Estevez, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
description Cell walls in the coenocytic green seaweed Codium vermilara (Olivi) Chiaje (Bryopsidales, Chlorophyta) are composed of ∼32% (w/w) β-(1→4)-d-mannans, ∼12% sulfated polysaccharides (SPs), and small amounts of hydroxyproline-rich glycoprotein-like (HRGP-L) compounds of the arabinogalactan proteins (AGPs) and arabinosides (extensins). Similar quantities of mannans and SPs were reported previously in the related seaweed C. fragile (Suringar) Hariot. Overall, both seaweed cell walls comprise ∼40%-44% of their dry weights. Within the SP group, a variety of polysaccharide structures from pyruvylated arabinogalactan sulfate and pyruvylated galactan sulfate to pyranosic arabinan sulfate are present in Codium cell walls. In this paper, the in situ distribution of the main cell-wall polymers in the green seaweed C. vermilara was studied, comparing their arrangements with those observed in cell walls from C. fragile. The utricle cell wall in C. vermilara showed by TEM a sandwich structure of two fibrillar-like layers of similar width delimiting a middle amorphous-like zone. By immuno- and chemical imaging, the in situ distribution of β-(1→4)-d-mannans and HRGP-like epitopes was shown to consist of two distinct cell-wall layers, whereas SPs are distributed in the middle area of the wall. The overall cell-wall polymer arrangement of the SPs, HRGP-like epitopes, and mannans in the utricles of C. vermilara is different from the ubiquitous green algae C. fragile, in spite of both being phylogenetically very close. In addition, a preliminary cell-wall model of the utricle moiety is proposed for both seaweeds, C. fragile and C.
publishDate 2010
dc.date.none.fl_str_mv 2010-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/277288
Fernández, Paula Virginia; Ciancia, Marina; Miravalles, Alicia Beatriz; Estevez, Jose Manuel; Cell wall polymer mapping in the coenocytic seaweed Codium vermilara (Bryopsidales, Chlorophyta); Wiley Blackwell Publishing, Inc; Journal of Phycology; 46; 3; 6-2010; 456-465
0022-3646
CONICET Digital
CONICET
url http://hdl.handle.net/11336/277288
identifier_str_mv Fernández, Paula Virginia; Ciancia, Marina; Miravalles, Alicia Beatriz; Estevez, Jose Manuel; Cell wall polymer mapping in the coenocytic seaweed Codium vermilara (Bryopsidales, Chlorophyta); Wiley Blackwell Publishing, Inc; Journal of Phycology; 46; 3; 6-2010; 456-465
0022-3646
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/j.1529-8817.2010.00821.x
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1529-8817.2010.00821.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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