Calbindin D 28k expression and abscence of apoptosis in the cerebellum of Solanum bonariense L intoxicated bovines

Autores
Verdes, José Manuel; Moraña, José Antonio; Battes, Daniel; Gutiérrez, Fernando; Guerrero, Florentina; Goicoa, Ana; Fidalgo, Luis Eusebio; Barbeito, Claudio Gustavo; Zanuzzi, Carolina Natalia; Portiansky, Enrique Leo; Gimeno, Eduardo Juan
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Solanum bonariense intoxication is characterized by cerebellar neuronal vacuolation, degeneration and necrosis. Cerebellar Purkinje cells seem especially susceptible but more research is needed to determine the pathogenesis of neuronal necrosis and mechanism of Purkinje cell susceptibility. Calbindin D 28k (CbD28k) is highly expressed in Purkinje cells, and it has been used as a specific marker of normal and degenerative Purkinje cells. The goals of the present work were to further describe S.bonariense induced disease by describing Purkinje cell specific degenerative changes using CbD28k expression correlated with the apoptotic changes determined using TUNEL and ultrastructural changes. Both dose and duration of S. bonariense intoxication decrease the number of Purkinje cells in all the studied areas. CbD28k immunohistochemistry was an excellent marker of Purkinje cells as immunoreactivity did not change in normal tissues or degenerative tissues. This suggests that rapid neuronal degeneration and death is not induced by excessive calcium excitatory stimulation. As found in previous studies, TUNEL tests and electron microscopy suggest Purkinje cell degeneration and death is not occurring via an apoptotic process. In summary these findings suggest that S.bonariense poisoning induces progressive Purkinje cell death that is not mediated by excitotoxicity or apoptotic activation.Solanum bonariense intoxication is characterized by cerebellar neuronal vacuolation, degeneration and necrosis. Cerebellar Purkinje cells seem especially susceptible but more research is needed to determine the pathogenesis of neuronal necrosis and mechanism of Purkinje cell susceptibility. Calbindin D 28k (CbD28k) is highly expressed in Purkinje cells, and it has been used as a specific marker of normal and degenerative Purkinje cells. The goals of the present work were to further describe S.bonariense induced disease by describing Purkinje cell specific degenerative changes using CbD28k expression correlated with the apoptotic changes determined using TUNEL and ultrastructural changes. Both dose and duration of S. bonariense intoxication decrease the number of Purkinje cells in all the studied areas. CbD28k immunohistochemistry was an excellent marker of Purkinje cells as immunoreactivity did not change in normal tissues or degenerative tissues. This suggests that rapid neuronal degeneration and death is not induced by excessive calcium excitatory stimulation. As found in previous studies, TUNEL tests and electron microscopy suggest Purkinje cell degeneration and death is not occurring via an apoptotic process. In summary these findings suggest that S.bonariense poisoning induces progressive Purkinje cell death that is not mediated by excitotoxicity or apoptotic activation.
Fil: Verdes, José Manuel. Universidad de la República; Uruguay
Fil: Moraña, José Antonio. Universidad de la República; Uruguay
Fil: Battes, Daniel. Universidad de la República; Uruguay
Fil: Gutiérrez, Fernando. Universidad de la República; Uruguay
Fil: Guerrero, Florentina. Universidad de Santiago de Compostela; España
Fil: Goicoa, Ana. Universidad de Santiago de Compostela; España
Fil: Fidalgo, Luis Eusebio. Universidad de Santiago de Compostela; España
Fil: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Zanuzzi, Carolina Natalia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Portiansky, Enrique Leo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Gimeno, Eduardo Juan. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Materia
Apoptosis
Calbindin D28k
Cattle
Cerebellar degeneration
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/254522
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/254522
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Calbindin D 28k expression and abscence of apoptosis in the cerebellum of Solanum bonariense L intoxicated bovinesVerdes, José ManuelMoraña, José AntonioBattes, DanielGutiérrez, FernandoGuerrero, FlorentinaGoicoa, AnaFidalgo, Luis EusebioBarbeito, Claudio GustavoZanuzzi, Carolina NataliaPortiansky, Enrique LeoGimeno, Eduardo JuanApoptosisCalbindin D28kCattleCerebellar degenerationhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Solanum bonariense intoxication is characterized by cerebellar neuronal vacuolation, degeneration and necrosis. Cerebellar Purkinje cells seem especially susceptible but more research is needed to determine the pathogenesis of neuronal necrosis and mechanism of Purkinje cell susceptibility. Calbindin D 28k (CbD28k) is highly expressed in Purkinje cells, and it has been used as a specific marker of normal and degenerative Purkinje cells. The goals of the present work were to further describe S.bonariense induced disease by describing Purkinje cell specific degenerative changes using CbD28k expression correlated with the apoptotic changes determined using TUNEL and ultrastructural changes. Both dose and duration of S. bonariense intoxication decrease the number of Purkinje cells in all the studied areas. CbD28k immunohistochemistry was an excellent marker of Purkinje cells as immunoreactivity did not change in normal tissues or degenerative tissues. This suggests that rapid neuronal degeneration and death is not induced by excessive calcium excitatory stimulation. As found in previous studies, TUNEL tests and electron microscopy suggest Purkinje cell degeneration and death is not occurring via an apoptotic process. In summary these findings suggest that S.bonariense poisoning induces progressive Purkinje cell death that is not mediated by excitotoxicity or apoptotic activation.Solanum bonariense intoxication is characterized by cerebellar neuronal vacuolation, degeneration and necrosis. Cerebellar Purkinje cells seem especially susceptible but more research is needed to determine the pathogenesis of neuronal necrosis and mechanism of Purkinje cell susceptibility. Calbindin D 28k (CbD28k) is highly expressed in Purkinje cells, and it has been used as a specific marker of normal and degenerative Purkinje cells. The goals of the present work were to further describe S.bonariense induced disease by describing Purkinje cell specific degenerative changes using CbD28k expression correlated with the apoptotic changes determined using TUNEL and ultrastructural changes. Both dose and duration of S. bonariense intoxication decrease the number of Purkinje cells in all the studied areas. CbD28k immunohistochemistry was an excellent marker of Purkinje cells as immunoreactivity did not change in normal tissues or degenerative tissues. This suggests that rapid neuronal degeneration and death is not induced by excessive calcium excitatory stimulation. As found in previous studies, TUNEL tests and electron microscopy suggest Purkinje cell degeneration and death is not occurring via an apoptotic process. In summary these findings suggest that S.bonariense poisoning induces progressive Purkinje cell death that is not mediated by excitotoxicity or apoptotic activation.Fil: Verdes, José Manuel. Universidad de la República; UruguayFil: Moraña, José Antonio. Universidad de la República; UruguayFil: Battes, Daniel. Universidad de la República; UruguayFil: Gutiérrez, Fernando. Universidad de la República; UruguayFil: Guerrero, Florentina. Universidad de Santiago de Compostela; EspañaFil: Goicoa, Ana. Universidad de Santiago de Compostela; EspañaFil: Fidalgo, Luis Eusebio. Universidad de Santiago de Compostela; EspañaFil: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Zanuzzi, Carolina Natalia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Portiansky, Enrique Leo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Gimeno, Eduardo Juan. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaSAGE Publications2010-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/254522Verdes, José Manuel; Moraña, José Antonio; Battes, Daniel; Gutiérrez, Fernando; Guerrero, Florentina; et al.; Calbindin D 28k expression and abscence of apoptosis in the cerebellum of Solanum bonariense L intoxicated bovines; SAGE Publications; Veterinary Pathology; 47; 3; 5-2010; 569-5720300-9858CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/full/10.1177/0300985809358040info:eu-repo/semantics/altIdentifier/doi/10.1177/0300985809358040info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:49Zoai:ri.conicet.gov.ar:11336/254522instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:50.26CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Calbindin D 28k expression and abscence of apoptosis in the cerebellum of Solanum bonariense L intoxicated bovines
title Calbindin D 28k expression and abscence of apoptosis in the cerebellum of Solanum bonariense L intoxicated bovines
spellingShingle Calbindin D 28k expression and abscence of apoptosis in the cerebellum of Solanum bonariense L intoxicated bovines
Verdes, José Manuel
Apoptosis
Calbindin D28k
Cattle
Cerebellar degeneration
title_short Calbindin D 28k expression and abscence of apoptosis in the cerebellum of Solanum bonariense L intoxicated bovines
title_full Calbindin D 28k expression and abscence of apoptosis in the cerebellum of Solanum bonariense L intoxicated bovines
title_fullStr Calbindin D 28k expression and abscence of apoptosis in the cerebellum of Solanum bonariense L intoxicated bovines
title_full_unstemmed Calbindin D 28k expression and abscence of apoptosis in the cerebellum of Solanum bonariense L intoxicated bovines
title_sort Calbindin D 28k expression and abscence of apoptosis in the cerebellum of Solanum bonariense L intoxicated bovines
dc.creator.none.fl_str_mv Verdes, José Manuel
Moraña, José Antonio
Battes, Daniel
Gutiérrez, Fernando
Guerrero, Florentina
Goicoa, Ana
Fidalgo, Luis Eusebio
Barbeito, Claudio Gustavo
Zanuzzi, Carolina Natalia
Portiansky, Enrique Leo
Gimeno, Eduardo Juan
author Verdes, José Manuel
author_facet Verdes, José Manuel
Moraña, José Antonio
Battes, Daniel
Gutiérrez, Fernando
Guerrero, Florentina
Goicoa, Ana
Fidalgo, Luis Eusebio
Barbeito, Claudio Gustavo
Zanuzzi, Carolina Natalia
Portiansky, Enrique Leo
Gimeno, Eduardo Juan
author_role author
author2 Moraña, José Antonio
Battes, Daniel
Gutiérrez, Fernando
Guerrero, Florentina
Goicoa, Ana
Fidalgo, Luis Eusebio
Barbeito, Claudio Gustavo
Zanuzzi, Carolina Natalia
Portiansky, Enrique Leo
Gimeno, Eduardo Juan
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Apoptosis
Calbindin D28k
Cattle
Cerebellar degeneration
topic Apoptosis
Calbindin D28k
Cattle
Cerebellar degeneration
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Solanum bonariense intoxication is characterized by cerebellar neuronal vacuolation, degeneration and necrosis. Cerebellar Purkinje cells seem especially susceptible but more research is needed to determine the pathogenesis of neuronal necrosis and mechanism of Purkinje cell susceptibility. Calbindin D 28k (CbD28k) is highly expressed in Purkinje cells, and it has been used as a specific marker of normal and degenerative Purkinje cells. The goals of the present work were to further describe S.bonariense induced disease by describing Purkinje cell specific degenerative changes using CbD28k expression correlated with the apoptotic changes determined using TUNEL and ultrastructural changes. Both dose and duration of S. bonariense intoxication decrease the number of Purkinje cells in all the studied areas. CbD28k immunohistochemistry was an excellent marker of Purkinje cells as immunoreactivity did not change in normal tissues or degenerative tissues. This suggests that rapid neuronal degeneration and death is not induced by excessive calcium excitatory stimulation. As found in previous studies, TUNEL tests and electron microscopy suggest Purkinje cell degeneration and death is not occurring via an apoptotic process. In summary these findings suggest that S.bonariense poisoning induces progressive Purkinje cell death that is not mediated by excitotoxicity or apoptotic activation.Solanum bonariense intoxication is characterized by cerebellar neuronal vacuolation, degeneration and necrosis. Cerebellar Purkinje cells seem especially susceptible but more research is needed to determine the pathogenesis of neuronal necrosis and mechanism of Purkinje cell susceptibility. Calbindin D 28k (CbD28k) is highly expressed in Purkinje cells, and it has been used as a specific marker of normal and degenerative Purkinje cells. The goals of the present work were to further describe S.bonariense induced disease by describing Purkinje cell specific degenerative changes using CbD28k expression correlated with the apoptotic changes determined using TUNEL and ultrastructural changes. Both dose and duration of S. bonariense intoxication decrease the number of Purkinje cells in all the studied areas. CbD28k immunohistochemistry was an excellent marker of Purkinje cells as immunoreactivity did not change in normal tissues or degenerative tissues. This suggests that rapid neuronal degeneration and death is not induced by excessive calcium excitatory stimulation. As found in previous studies, TUNEL tests and electron microscopy suggest Purkinje cell degeneration and death is not occurring via an apoptotic process. In summary these findings suggest that S.bonariense poisoning induces progressive Purkinje cell death that is not mediated by excitotoxicity or apoptotic activation.
Fil: Verdes, José Manuel. Universidad de la República; Uruguay
Fil: Moraña, José Antonio. Universidad de la República; Uruguay
Fil: Battes, Daniel. Universidad de la República; Uruguay
Fil: Gutiérrez, Fernando. Universidad de la República; Uruguay
Fil: Guerrero, Florentina. Universidad de Santiago de Compostela; España
Fil: Goicoa, Ana. Universidad de Santiago de Compostela; España
Fil: Fidalgo, Luis Eusebio. Universidad de Santiago de Compostela; España
Fil: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Zanuzzi, Carolina Natalia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Portiansky, Enrique Leo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Gimeno, Eduardo Juan. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
description Solanum bonariense intoxication is characterized by cerebellar neuronal vacuolation, degeneration and necrosis. Cerebellar Purkinje cells seem especially susceptible but more research is needed to determine the pathogenesis of neuronal necrosis and mechanism of Purkinje cell susceptibility. Calbindin D 28k (CbD28k) is highly expressed in Purkinje cells, and it has been used as a specific marker of normal and degenerative Purkinje cells. The goals of the present work were to further describe S.bonariense induced disease by describing Purkinje cell specific degenerative changes using CbD28k expression correlated with the apoptotic changes determined using TUNEL and ultrastructural changes. Both dose and duration of S. bonariense intoxication decrease the number of Purkinje cells in all the studied areas. CbD28k immunohistochemistry was an excellent marker of Purkinje cells as immunoreactivity did not change in normal tissues or degenerative tissues. This suggests that rapid neuronal degeneration and death is not induced by excessive calcium excitatory stimulation. As found in previous studies, TUNEL tests and electron microscopy suggest Purkinje cell degeneration and death is not occurring via an apoptotic process. In summary these findings suggest that S.bonariense poisoning induces progressive Purkinje cell death that is not mediated by excitotoxicity or apoptotic activation.Solanum bonariense intoxication is characterized by cerebellar neuronal vacuolation, degeneration and necrosis. Cerebellar Purkinje cells seem especially susceptible but more research is needed to determine the pathogenesis of neuronal necrosis and mechanism of Purkinje cell susceptibility. Calbindin D 28k (CbD28k) is highly expressed in Purkinje cells, and it has been used as a specific marker of normal and degenerative Purkinje cells. The goals of the present work were to further describe S.bonariense induced disease by describing Purkinje cell specific degenerative changes using CbD28k expression correlated with the apoptotic changes determined using TUNEL and ultrastructural changes. Both dose and duration of S. bonariense intoxication decrease the number of Purkinje cells in all the studied areas. CbD28k immunohistochemistry was an excellent marker of Purkinje cells as immunoreactivity did not change in normal tissues or degenerative tissues. This suggests that rapid neuronal degeneration and death is not induced by excessive calcium excitatory stimulation. As found in previous studies, TUNEL tests and electron microscopy suggest Purkinje cell degeneration and death is not occurring via an apoptotic process. In summary these findings suggest that S.bonariense poisoning induces progressive Purkinje cell death that is not mediated by excitotoxicity or apoptotic activation.
publishDate 2010
dc.date.none.fl_str_mv 2010-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/254522
Verdes, José Manuel; Moraña, José Antonio; Battes, Daniel; Gutiérrez, Fernando; Guerrero, Florentina; et al.; Calbindin D 28k expression and abscence of apoptosis in the cerebellum of Solanum bonariense L intoxicated bovines; SAGE Publications; Veterinary Pathology; 47; 3; 5-2010; 569-572
0300-9858
CONICET Digital
CONICET
url http://hdl.handle.net/11336/254522
identifier_str_mv Verdes, José Manuel; Moraña, José Antonio; Battes, Daniel; Gutiérrez, Fernando; Guerrero, Florentina; et al.; Calbindin D 28k expression and abscence of apoptosis in the cerebellum of Solanum bonariense L intoxicated bovines; SAGE Publications; Veterinary Pathology; 47; 3; 5-2010; 569-572
0300-9858
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
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dc.publisher.none.fl_str_mv SAGE Publications
publisher.none.fl_str_mv SAGE Publications
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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