Changes in Ca2+ removal can mask the effects of geometry during IP3R mediated Ca2+ signals

Autores
Piegari, Estefanía; Villarruel, Cecilia Liliana; Ponce Dawson, Silvina Martha
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Calcium (Ca2+) signals are ubiquitous. Most intracellular Ca2+ signals involve the release of Ca2+ from the endoplasmic reticulum (ER) through Inositol 1,4,5-Trisphosphate Receptors (IP3Rs). The non-uniform spatial organization of IP3Rs and the fact that their individual openings are coupled via cytosolic Ca2+ are key factors for the variety of spatio-temporal distributions of the cytosolic [Ca2+] and the versatility of the signals. In this paper we combine experiments performed in untreated and in progesterone-treated Xenopus laevis oocytes and mathematical models to investigate how the interplay between geometry (the IP3R spatial distribution) and dynamics (the processes that characterize the release, transport, and removal of cytosolic Ca2+) affects the resulting signals. Signal propagation looks more continuous and spatially uniform in treated (mature) than in untreated (immature) oocytes. This could be due to the different underlying IP3R spatial distribution that has been observed in both cell types. The models, however, show that the rate of cytosolic Ca2+ removal, which is also different in both cell types, plays a key role affecting the coupling between Ca2+ release sites in such a way that the effect of the underlying IP3R spatial distribution can be modified.
Fil: Piegari, Estefanía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Fil: Villarruel, Cecilia Liliana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Fil: Ponce Dawson, Silvina Martha. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Materia
BUFFERS
CALCIUM SIGNALING
IP3R DISTRIBUTION
OOCYTE MATURATION
SPATIOTEMPORAL DISTRIBUTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/147739
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Changes in Ca2+ removal can mask the effects of geometry during IP3R mediated Ca2+ signalsPiegari, EstefaníaVillarruel, Cecilia LilianaPonce Dawson, Silvina MarthaBUFFERSCALCIUM SIGNALINGIP3R DISTRIBUTIONOOCYTE MATURATIONSPATIOTEMPORAL DISTRIBUTIONhttps://purl.org/becyt/ford/1.3https://purl.org/becyt/ford/1Calcium (Ca2+) signals are ubiquitous. Most intracellular Ca2+ signals involve the release of Ca2+ from the endoplasmic reticulum (ER) through Inositol 1,4,5-Trisphosphate Receptors (IP3Rs). The non-uniform spatial organization of IP3Rs and the fact that their individual openings are coupled via cytosolic Ca2+ are key factors for the variety of spatio-temporal distributions of the cytosolic [Ca2+] and the versatility of the signals. In this paper we combine experiments performed in untreated and in progesterone-treated Xenopus laevis oocytes and mathematical models to investigate how the interplay between geometry (the IP3R spatial distribution) and dynamics (the processes that characterize the release, transport, and removal of cytosolic Ca2+) affects the resulting signals. Signal propagation looks more continuous and spatially uniform in treated (mature) than in untreated (immature) oocytes. This could be due to the different underlying IP3R spatial distribution that has been observed in both cell types. The models, however, show that the rate of cytosolic Ca2+ removal, which is also different in both cell types, plays a key role affecting the coupling between Ca2+ release sites in such a way that the effect of the underlying IP3R spatial distribution can be modified.Fil: Piegari, Estefanía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; ArgentinaFil: Villarruel, Cecilia Liliana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; ArgentinaFil: Ponce Dawson, Silvina Martha. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; ArgentinaFrontiers Media2019-07-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/147739Piegari, Estefanía; Villarruel, Cecilia Liliana; Ponce Dawson, Silvina Martha; Changes in Ca2+ removal can mask the effects of geometry during IP3R mediated Ca2+ signals; Frontiers Media; Frontiers in Physiology; 10; 31-7-2019; 1-161664-042XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fphys.2019.00964/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fphys.2019.00964info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:44:36Zoai:ri.conicet.gov.ar:11336/147739instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:44:36.796CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Changes in Ca2+ removal can mask the effects of geometry during IP3R mediated Ca2+ signals
title Changes in Ca2+ removal can mask the effects of geometry during IP3R mediated Ca2+ signals
spellingShingle Changes in Ca2+ removal can mask the effects of geometry during IP3R mediated Ca2+ signals
Piegari, Estefanía
BUFFERS
CALCIUM SIGNALING
IP3R DISTRIBUTION
OOCYTE MATURATION
SPATIOTEMPORAL DISTRIBUTION
title_short Changes in Ca2+ removal can mask the effects of geometry during IP3R mediated Ca2+ signals
title_full Changes in Ca2+ removal can mask the effects of geometry during IP3R mediated Ca2+ signals
title_fullStr Changes in Ca2+ removal can mask the effects of geometry during IP3R mediated Ca2+ signals
title_full_unstemmed Changes in Ca2+ removal can mask the effects of geometry during IP3R mediated Ca2+ signals
title_sort Changes in Ca2+ removal can mask the effects of geometry during IP3R mediated Ca2+ signals
dc.creator.none.fl_str_mv Piegari, Estefanía
Villarruel, Cecilia Liliana
Ponce Dawson, Silvina Martha
author Piegari, Estefanía
author_facet Piegari, Estefanía
Villarruel, Cecilia Liliana
Ponce Dawson, Silvina Martha
author_role author
author2 Villarruel, Cecilia Liliana
Ponce Dawson, Silvina Martha
author2_role author
author
dc.subject.none.fl_str_mv BUFFERS
CALCIUM SIGNALING
IP3R DISTRIBUTION
OOCYTE MATURATION
SPATIOTEMPORAL DISTRIBUTION
topic BUFFERS
CALCIUM SIGNALING
IP3R DISTRIBUTION
OOCYTE MATURATION
SPATIOTEMPORAL DISTRIBUTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.3
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Calcium (Ca2+) signals are ubiquitous. Most intracellular Ca2+ signals involve the release of Ca2+ from the endoplasmic reticulum (ER) through Inositol 1,4,5-Trisphosphate Receptors (IP3Rs). The non-uniform spatial organization of IP3Rs and the fact that their individual openings are coupled via cytosolic Ca2+ are key factors for the variety of spatio-temporal distributions of the cytosolic [Ca2+] and the versatility of the signals. In this paper we combine experiments performed in untreated and in progesterone-treated Xenopus laevis oocytes and mathematical models to investigate how the interplay between geometry (the IP3R spatial distribution) and dynamics (the processes that characterize the release, transport, and removal of cytosolic Ca2+) affects the resulting signals. Signal propagation looks more continuous and spatially uniform in treated (mature) than in untreated (immature) oocytes. This could be due to the different underlying IP3R spatial distribution that has been observed in both cell types. The models, however, show that the rate of cytosolic Ca2+ removal, which is also different in both cell types, plays a key role affecting the coupling between Ca2+ release sites in such a way that the effect of the underlying IP3R spatial distribution can be modified.
Fil: Piegari, Estefanía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Fil: Villarruel, Cecilia Liliana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Fil: Ponce Dawson, Silvina Martha. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
description Calcium (Ca2+) signals are ubiquitous. Most intracellular Ca2+ signals involve the release of Ca2+ from the endoplasmic reticulum (ER) through Inositol 1,4,5-Trisphosphate Receptors (IP3Rs). The non-uniform spatial organization of IP3Rs and the fact that their individual openings are coupled via cytosolic Ca2+ are key factors for the variety of spatio-temporal distributions of the cytosolic [Ca2+] and the versatility of the signals. In this paper we combine experiments performed in untreated and in progesterone-treated Xenopus laevis oocytes and mathematical models to investigate how the interplay between geometry (the IP3R spatial distribution) and dynamics (the processes that characterize the release, transport, and removal of cytosolic Ca2+) affects the resulting signals. Signal propagation looks more continuous and spatially uniform in treated (mature) than in untreated (immature) oocytes. This could be due to the different underlying IP3R spatial distribution that has been observed in both cell types. The models, however, show that the rate of cytosolic Ca2+ removal, which is also different in both cell types, plays a key role affecting the coupling between Ca2+ release sites in such a way that the effect of the underlying IP3R spatial distribution can be modified.
publishDate 2019
dc.date.none.fl_str_mv 2019-07-31
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/147739
Piegari, Estefanía; Villarruel, Cecilia Liliana; Ponce Dawson, Silvina Martha; Changes in Ca2+ removal can mask the effects of geometry during IP3R mediated Ca2+ signals; Frontiers Media; Frontiers in Physiology; 10; 31-7-2019; 1-16
1664-042X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/147739
identifier_str_mv Piegari, Estefanía; Villarruel, Cecilia Liliana; Ponce Dawson, Silvina Martha; Changes in Ca2+ removal can mask the effects of geometry during IP3R mediated Ca2+ signals; Frontiers Media; Frontiers in Physiology; 10; 31-7-2019; 1-16
1664-042X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fphys.2019.00964/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fphys.2019.00964
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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