Functional Ca2+ Channels between Channel Clusters are Necessary for the Propagation of IP3R-Mediated Ca2+ Waves

Autores
Piegari, Estefanía; Ponce Dawson, Silvina Martha
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The specificity and universality of intracellular Ca2+ signals rely on the variety of spatio-temporal patterns that the Ca2+ concentration can display. Ca2+ release into the cytosol through inositol 1,4,5-trisphosphate receptors (IP 3 Rs) is key for this variety. The opening probability of IP3Rs depends on the cytosolic Ca2+ concentration. All of the dynamics are then well described by an excitable system in which the signal propagation depends on the ability of the Ca2+ released through one IP3R to induce the opening of other IP3Rs. In most cell types, IP3Rs are organized in clusters, i.e., the cytosol is a "patchy" excitable system in which the signals can remain localized (i.e., involving the release through one or more IP3Rs in a cluster), or become global depending on the efficiency of the Ca2+ -mediated coupling between clusters. The spatial range over which the signals propagate determines the responses that the cell eventually produces. This points to the importance of understanding the mechanisms that make the propagation possible. Our previous qualitative comparison between experiments and numerical simulations seemed to indicate that Ca2+ release not only occurs within the close vicinity of the clearly identifiable release sites (IP3R clusters) but that there are also functional IP3Rs in between them. In this paper, we present a quantitative comparison between experiments and models that corroborate this preliminary conclusion. This result has implications on how the Ca2+-mediated coupling between clusters works and how it can eventually be disrupted by the different Ca2+ trapping mechanisms.
Fil: Piegari, Estefanía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Fil: Ponce Dawson, Silvina Martha. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Materia
CALCIUM
IP3R
CALCIUM-INDUCED-CALCIUM-RELEASE
WAVES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/121079

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spelling Functional Ca2+ Channels between Channel Clusters are Necessary for the Propagation of IP3R-Mediated Ca2+ WavesPiegari, EstefaníaPonce Dawson, Silvina MarthaCALCIUMIP3RCALCIUM-INDUCED-CALCIUM-RELEASEWAVEShttps://purl.org/becyt/ford/1.3https://purl.org/becyt/ford/1The specificity and universality of intracellular Ca2+ signals rely on the variety of spatio-temporal patterns that the Ca2+ concentration can display. Ca2+ release into the cytosol through inositol 1,4,5-trisphosphate receptors (IP 3 Rs) is key for this variety. The opening probability of IP3Rs depends on the cytosolic Ca2+ concentration. All of the dynamics are then well described by an excitable system in which the signal propagation depends on the ability of the Ca2+ released through one IP3R to induce the opening of other IP3Rs. In most cell types, IP3Rs are organized in clusters, i.e., the cytosol is a "patchy" excitable system in which the signals can remain localized (i.e., involving the release through one or more IP3Rs in a cluster), or become global depending on the efficiency of the Ca2+ -mediated coupling between clusters. The spatial range over which the signals propagate determines the responses that the cell eventually produces. This points to the importance of understanding the mechanisms that make the propagation possible. Our previous qualitative comparison between experiments and numerical simulations seemed to indicate that Ca2+ release not only occurs within the close vicinity of the clearly identifiable release sites (IP3R clusters) but that there are also functional IP3Rs in between them. In this paper, we present a quantitative comparison between experiments and models that corroborate this preliminary conclusion. This result has implications on how the Ca2+-mediated coupling between clusters works and how it can eventually be disrupted by the different Ca2+ trapping mechanisms.Fil: Piegari, Estefanía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; ArgentinaFil: Ponce Dawson, Silvina Martha. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; ArgentinaMultidisciplinary Digital Publishing Institute2019-06-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/121079Piegari, Estefanía; Ponce Dawson, Silvina Martha; Functional Ca2+ Channels between Channel Clusters are Necessary for the Propagation of IP3R-Mediated Ca2+ Waves; Multidisciplinary Digital Publishing Institute; Mathematical and Computational Applications; 24; 2; 11-6-2019; 1-142297-8747CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2297-8747/24/2/61info:eu-repo/semantics/altIdentifier/doi/ 10.3390/mca24020061info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:47:12Zoai:ri.conicet.gov.ar:11336/121079instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:47:13.037CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Functional Ca2+ Channels between Channel Clusters are Necessary for the Propagation of IP3R-Mediated Ca2+ Waves
title Functional Ca2+ Channels between Channel Clusters are Necessary for the Propagation of IP3R-Mediated Ca2+ Waves
spellingShingle Functional Ca2+ Channels between Channel Clusters are Necessary for the Propagation of IP3R-Mediated Ca2+ Waves
Piegari, Estefanía
CALCIUM
IP3R
CALCIUM-INDUCED-CALCIUM-RELEASE
WAVES
title_short Functional Ca2+ Channels between Channel Clusters are Necessary for the Propagation of IP3R-Mediated Ca2+ Waves
title_full Functional Ca2+ Channels between Channel Clusters are Necessary for the Propagation of IP3R-Mediated Ca2+ Waves
title_fullStr Functional Ca2+ Channels between Channel Clusters are Necessary for the Propagation of IP3R-Mediated Ca2+ Waves
title_full_unstemmed Functional Ca2+ Channels between Channel Clusters are Necessary for the Propagation of IP3R-Mediated Ca2+ Waves
title_sort Functional Ca2+ Channels between Channel Clusters are Necessary for the Propagation of IP3R-Mediated Ca2+ Waves
dc.creator.none.fl_str_mv Piegari, Estefanía
Ponce Dawson, Silvina Martha
author Piegari, Estefanía
author_facet Piegari, Estefanía
Ponce Dawson, Silvina Martha
author_role author
author2 Ponce Dawson, Silvina Martha
author2_role author
dc.subject.none.fl_str_mv CALCIUM
IP3R
CALCIUM-INDUCED-CALCIUM-RELEASE
WAVES
topic CALCIUM
IP3R
CALCIUM-INDUCED-CALCIUM-RELEASE
WAVES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.3
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The specificity and universality of intracellular Ca2+ signals rely on the variety of spatio-temporal patterns that the Ca2+ concentration can display. Ca2+ release into the cytosol through inositol 1,4,5-trisphosphate receptors (IP 3 Rs) is key for this variety. The opening probability of IP3Rs depends on the cytosolic Ca2+ concentration. All of the dynamics are then well described by an excitable system in which the signal propagation depends on the ability of the Ca2+ released through one IP3R to induce the opening of other IP3Rs. In most cell types, IP3Rs are organized in clusters, i.e., the cytosol is a "patchy" excitable system in which the signals can remain localized (i.e., involving the release through one or more IP3Rs in a cluster), or become global depending on the efficiency of the Ca2+ -mediated coupling between clusters. The spatial range over which the signals propagate determines the responses that the cell eventually produces. This points to the importance of understanding the mechanisms that make the propagation possible. Our previous qualitative comparison between experiments and numerical simulations seemed to indicate that Ca2+ release not only occurs within the close vicinity of the clearly identifiable release sites (IP3R clusters) but that there are also functional IP3Rs in between them. In this paper, we present a quantitative comparison between experiments and models that corroborate this preliminary conclusion. This result has implications on how the Ca2+-mediated coupling between clusters works and how it can eventually be disrupted by the different Ca2+ trapping mechanisms.
Fil: Piegari, Estefanía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Fil: Ponce Dawson, Silvina Martha. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
description The specificity and universality of intracellular Ca2+ signals rely on the variety of spatio-temporal patterns that the Ca2+ concentration can display. Ca2+ release into the cytosol through inositol 1,4,5-trisphosphate receptors (IP 3 Rs) is key for this variety. The opening probability of IP3Rs depends on the cytosolic Ca2+ concentration. All of the dynamics are then well described by an excitable system in which the signal propagation depends on the ability of the Ca2+ released through one IP3R to induce the opening of other IP3Rs. In most cell types, IP3Rs are organized in clusters, i.e., the cytosol is a "patchy" excitable system in which the signals can remain localized (i.e., involving the release through one or more IP3Rs in a cluster), or become global depending on the efficiency of the Ca2+ -mediated coupling between clusters. The spatial range over which the signals propagate determines the responses that the cell eventually produces. This points to the importance of understanding the mechanisms that make the propagation possible. Our previous qualitative comparison between experiments and numerical simulations seemed to indicate that Ca2+ release not only occurs within the close vicinity of the clearly identifiable release sites (IP3R clusters) but that there are also functional IP3Rs in between them. In this paper, we present a quantitative comparison between experiments and models that corroborate this preliminary conclusion. This result has implications on how the Ca2+-mediated coupling between clusters works and how it can eventually be disrupted by the different Ca2+ trapping mechanisms.
publishDate 2019
dc.date.none.fl_str_mv 2019-06-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/121079
Piegari, Estefanía; Ponce Dawson, Silvina Martha; Functional Ca2+ Channels between Channel Clusters are Necessary for the Propagation of IP3R-Mediated Ca2+ Waves; Multidisciplinary Digital Publishing Institute; Mathematical and Computational Applications; 24; 2; 11-6-2019; 1-14
2297-8747
CONICET Digital
CONICET
url http://hdl.handle.net/11336/121079
identifier_str_mv Piegari, Estefanía; Ponce Dawson, Silvina Martha; Functional Ca2+ Channels between Channel Clusters are Necessary for the Propagation of IP3R-Mediated Ca2+ Waves; Multidisciplinary Digital Publishing Institute; Mathematical and Computational Applications; 24; 2; 11-6-2019; 1-14
2297-8747
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2297-8747/24/2/61
info:eu-repo/semantics/altIdentifier/doi/ 10.3390/mca24020061
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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