Lactobacillus delbrueckii crl 581 differentially modulates tlr3-triggered antiviral innate immune response in intestinal epithelial cells and macrophages

Autores
Elean, Mariano Daniel; Albarracín, Leonardo Miguel; Fukuyama, Kohtaro; Zhou, Binghui; Tomokiyo, Mikado; Kitahara, Shugo; Araki, Shota; Suda, Yoshihito; Saavedra, Maria Lucila; Villena, Julio Cesar; Hebert, Elvira Maria; Kitazawa, Haruki
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lactobacillus delbrueckii subsp. lactis CRL 581 beneficially modulates the intestinal antiviral innate immune response triggered by the Toll-like receptor 3 (TLR3) agonist poly(I:C) in vivo. This study aimed to characterize further the immunomodulatory properties of the technologically relevant starter culture L. delbrueckii subsp. lactis CRL 581 by evaluating its interaction with intestinal epithelial cells and macrophages in the context of innate immune responses triggered by TLR3. Our results showed that the CRL 581 strain was able to adhere to porcine intestinal epithelial (PIE) cells and mucins. The CRL 581 strain also augmented the expression of antiviral factors (IFN-α, IFN-β, Mx1, OAS1, and OAS2) and reduced inflammatory cytokines in PIE cells triggered by TLR3 stimulation. In addition, the influence of L. delbrueckii subsp. lactis CRL 581 on the response of murine RAW macrophages to the activation of TLR3 was evaluated. The CRL 581 strain was capable of enhancing the expression of IFN-α, IFN-β, IFN-γ, Mx1, OAS1, TNF-α, and IL-1β. Of note, the CRL 581 strain also augmented the expression of IL-10 in macrophages. The results of this study show that the high proteolytic strain L. delbrueckii spp. lactis CRL 581 was able to beneficially modulate the intestinal innate antiviral immune response by regulating the response of both epithelial cells and macrophages relative to TLR3 activation.
Fil: Elean, Mariano Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Fukuyama, Kohtaro. Tohoku University; Japón
Fil: Zhou, Binghui. Tohoku University; Japón
Fil: Tomokiyo, Mikado. Tohoku University; Japón
Fil: Kitahara, Shugo. Tohoku University; Japón
Fil: Araki, Shota. Tohoku University; Japón
Fil: Suda, Yoshihito. Miyagi University; Japón
Fil: Saavedra, Maria Lucila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Hebert, Elvira Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Kitazawa, Haruki. Tohoku University; Japón
Materia
ANTIVIRAL RESPONSE
IMMUNOBIOTICS
INTESTINAL EPITHELIAL CELLS
LACTOBACILLUS DELBRUECKII SUBSP. LACTIS CRL 581
MACROPHAGES
TLR3
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/153658
Acceder
id CONICETDig_98119f06bc7feddf7797691a720eed54
oai_identifier_str oai:ri.conicet.gov.ar:11336/153658
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Lactobacillus delbrueckii crl 581 differentially modulates tlr3-triggered antiviral innate immune response in intestinal epithelial cells and macrophagesElean, Mariano DanielAlbarracín, Leonardo MiguelFukuyama, KohtaroZhou, BinghuiTomokiyo, MikadoKitahara, ShugoAraki, ShotaSuda, YoshihitoSaavedra, Maria LucilaVillena, Julio CesarHebert, Elvira MariaKitazawa, HarukiANTIVIRAL RESPONSEIMMUNOBIOTICSINTESTINAL EPITHELIAL CELLSLACTOBACILLUS DELBRUECKII SUBSP. LACTIS CRL 581MACROPHAGESTLR3https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Lactobacillus delbrueckii subsp. lactis CRL 581 beneficially modulates the intestinal antiviral innate immune response triggered by the Toll-like receptor 3 (TLR3) agonist poly(I:C) in vivo. This study aimed to characterize further the immunomodulatory properties of the technologically relevant starter culture L. delbrueckii subsp. lactis CRL 581 by evaluating its interaction with intestinal epithelial cells and macrophages in the context of innate immune responses triggered by TLR3. Our results showed that the CRL 581 strain was able to adhere to porcine intestinal epithelial (PIE) cells and mucins. The CRL 581 strain also augmented the expression of antiviral factors (IFN-α, IFN-β, Mx1, OAS1, and OAS2) and reduced inflammatory cytokines in PIE cells triggered by TLR3 stimulation. In addition, the influence of L. delbrueckii subsp. lactis CRL 581 on the response of murine RAW macrophages to the activation of TLR3 was evaluated. The CRL 581 strain was capable of enhancing the expression of IFN-α, IFN-β, IFN-γ, Mx1, OAS1, TNF-α, and IL-1β. Of note, the CRL 581 strain also augmented the expression of IL-10 in macrophages. The results of this study show that the high proteolytic strain L. delbrueckii spp. lactis CRL 581 was able to beneficially modulate the intestinal innate antiviral immune response by regulating the response of both epithelial cells and macrophages relative to TLR3 activation.Fil: Elean, Mariano Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Fukuyama, Kohtaro. Tohoku University; JapónFil: Zhou, Binghui. Tohoku University; JapónFil: Tomokiyo, Mikado. Tohoku University; JapónFil: Kitahara, Shugo. Tohoku University; JapónFil: Araki, Shota. Tohoku University; JapónFil: Suda, Yoshihito. Miyagi University; JapónFil: Saavedra, Maria Lucila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Hebert, Elvira Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Kitazawa, Haruki. Tohoku University; JapónMDPI2021-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/153658Elean, Mariano Daniel; Albarracín, Leonardo Miguel; Fukuyama, Kohtaro; Zhou, Binghui; Tomokiyo, Mikado; et al.; Lactobacillus delbrueckii crl 581 differentially modulates tlr3-triggered antiviral innate immune response in intestinal epithelial cells and macrophages; MDPI; Microorganisms; 9; 12; 11-2021; 1-182076-2607CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms9122449info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-2607/9/12/2449info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:44Zoai:ri.conicet.gov.ar:11336/153658instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:44.783CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Lactobacillus delbrueckii crl 581 differentially modulates tlr3-triggered antiviral innate immune response in intestinal epithelial cells and macrophages
title Lactobacillus delbrueckii crl 581 differentially modulates tlr3-triggered antiviral innate immune response in intestinal epithelial cells and macrophages
spellingShingle Lactobacillus delbrueckii crl 581 differentially modulates tlr3-triggered antiviral innate immune response in intestinal epithelial cells and macrophages
Elean, Mariano Daniel
ANTIVIRAL RESPONSE
IMMUNOBIOTICS
INTESTINAL EPITHELIAL CELLS
LACTOBACILLUS DELBRUECKII SUBSP. LACTIS CRL 581
MACROPHAGES
TLR3
title_short Lactobacillus delbrueckii crl 581 differentially modulates tlr3-triggered antiviral innate immune response in intestinal epithelial cells and macrophages
title_full Lactobacillus delbrueckii crl 581 differentially modulates tlr3-triggered antiviral innate immune response in intestinal epithelial cells and macrophages
title_fullStr Lactobacillus delbrueckii crl 581 differentially modulates tlr3-triggered antiviral innate immune response in intestinal epithelial cells and macrophages
title_full_unstemmed Lactobacillus delbrueckii crl 581 differentially modulates tlr3-triggered antiviral innate immune response in intestinal epithelial cells and macrophages
title_sort Lactobacillus delbrueckii crl 581 differentially modulates tlr3-triggered antiviral innate immune response in intestinal epithelial cells and macrophages
dc.creator.none.fl_str_mv Elean, Mariano Daniel
Albarracín, Leonardo Miguel
Fukuyama, Kohtaro
Zhou, Binghui
Tomokiyo, Mikado
Kitahara, Shugo
Araki, Shota
Suda, Yoshihito
Saavedra, Maria Lucila
Villena, Julio Cesar
Hebert, Elvira Maria
Kitazawa, Haruki
author Elean, Mariano Daniel
author_facet Elean, Mariano Daniel
Albarracín, Leonardo Miguel
Fukuyama, Kohtaro
Zhou, Binghui
Tomokiyo, Mikado
Kitahara, Shugo
Araki, Shota
Suda, Yoshihito
Saavedra, Maria Lucila
Villena, Julio Cesar
Hebert, Elvira Maria
Kitazawa, Haruki
author_role author
author2 Albarracín, Leonardo Miguel
Fukuyama, Kohtaro
Zhou, Binghui
Tomokiyo, Mikado
Kitahara, Shugo
Araki, Shota
Suda, Yoshihito
Saavedra, Maria Lucila
Villena, Julio Cesar
Hebert, Elvira Maria
Kitazawa, Haruki
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANTIVIRAL RESPONSE
IMMUNOBIOTICS
INTESTINAL EPITHELIAL CELLS
LACTOBACILLUS DELBRUECKII SUBSP. LACTIS CRL 581
MACROPHAGES
TLR3
topic ANTIVIRAL RESPONSE
IMMUNOBIOTICS
INTESTINAL EPITHELIAL CELLS
LACTOBACILLUS DELBRUECKII SUBSP. LACTIS CRL 581
MACROPHAGES
TLR3
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Lactobacillus delbrueckii subsp. lactis CRL 581 beneficially modulates the intestinal antiviral innate immune response triggered by the Toll-like receptor 3 (TLR3) agonist poly(I:C) in vivo. This study aimed to characterize further the immunomodulatory properties of the technologically relevant starter culture L. delbrueckii subsp. lactis CRL 581 by evaluating its interaction with intestinal epithelial cells and macrophages in the context of innate immune responses triggered by TLR3. Our results showed that the CRL 581 strain was able to adhere to porcine intestinal epithelial (PIE) cells and mucins. The CRL 581 strain also augmented the expression of antiviral factors (IFN-α, IFN-β, Mx1, OAS1, and OAS2) and reduced inflammatory cytokines in PIE cells triggered by TLR3 stimulation. In addition, the influence of L. delbrueckii subsp. lactis CRL 581 on the response of murine RAW macrophages to the activation of TLR3 was evaluated. The CRL 581 strain was capable of enhancing the expression of IFN-α, IFN-β, IFN-γ, Mx1, OAS1, TNF-α, and IL-1β. Of note, the CRL 581 strain also augmented the expression of IL-10 in macrophages. The results of this study show that the high proteolytic strain L. delbrueckii spp. lactis CRL 581 was able to beneficially modulate the intestinal innate antiviral immune response by regulating the response of both epithelial cells and macrophages relative to TLR3 activation.
Fil: Elean, Mariano Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Fukuyama, Kohtaro. Tohoku University; Japón
Fil: Zhou, Binghui. Tohoku University; Japón
Fil: Tomokiyo, Mikado. Tohoku University; Japón
Fil: Kitahara, Shugo. Tohoku University; Japón
Fil: Araki, Shota. Tohoku University; Japón
Fil: Suda, Yoshihito. Miyagi University; Japón
Fil: Saavedra, Maria Lucila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Hebert, Elvira Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Kitazawa, Haruki. Tohoku University; Japón
description Lactobacillus delbrueckii subsp. lactis CRL 581 beneficially modulates the intestinal antiviral innate immune response triggered by the Toll-like receptor 3 (TLR3) agonist poly(I:C) in vivo. This study aimed to characterize further the immunomodulatory properties of the technologically relevant starter culture L. delbrueckii subsp. lactis CRL 581 by evaluating its interaction with intestinal epithelial cells and macrophages in the context of innate immune responses triggered by TLR3. Our results showed that the CRL 581 strain was able to adhere to porcine intestinal epithelial (PIE) cells and mucins. The CRL 581 strain also augmented the expression of antiviral factors (IFN-α, IFN-β, Mx1, OAS1, and OAS2) and reduced inflammatory cytokines in PIE cells triggered by TLR3 stimulation. In addition, the influence of L. delbrueckii subsp. lactis CRL 581 on the response of murine RAW macrophages to the activation of TLR3 was evaluated. The CRL 581 strain was capable of enhancing the expression of IFN-α, IFN-β, IFN-γ, Mx1, OAS1, TNF-α, and IL-1β. Of note, the CRL 581 strain also augmented the expression of IL-10 in macrophages. The results of this study show that the high proteolytic strain L. delbrueckii spp. lactis CRL 581 was able to beneficially modulate the intestinal innate antiviral immune response by regulating the response of both epithelial cells and macrophages relative to TLR3 activation.
publishDate 2021
dc.date.none.fl_str_mv 2021-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/153658
Elean, Mariano Daniel; Albarracín, Leonardo Miguel; Fukuyama, Kohtaro; Zhou, Binghui; Tomokiyo, Mikado; et al.; Lactobacillus delbrueckii crl 581 differentially modulates tlr3-triggered antiviral innate immune response in intestinal epithelial cells and macrophages; MDPI; Microorganisms; 9; 12; 11-2021; 1-18
2076-2607
CONICET Digital
CONICET
url http://hdl.handle.net/11336/153658
identifier_str_mv Elean, Mariano Daniel; Albarracín, Leonardo Miguel; Fukuyama, Kohtaro; Zhou, Binghui; Tomokiyo, Mikado; et al.; Lactobacillus delbrueckii crl 581 differentially modulates tlr3-triggered antiviral innate immune response in intestinal epithelial cells and macrophages; MDPI; Microorganisms; 9; 12; 11-2021; 1-18
2076-2607
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms9122449
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-2607/9/12/2449
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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