Immunobiotic Lactobacillus jenseni TL2937 alleviates dextran sodium sulfate-induced colitis in mice

Autores
Islam, M. Aminul; Garcia Castillo, Valeria; Sato, Nana; Albarracín, Leonardo Miguel; Kitazawa, Haruki; Villena, Julio Cesar
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Immunobiotics have emerged as promising interventions to alleviate inflammatorydamage in inflammatory bowel disease (IBD). However, the beneficial properties of immunobioticsare strain dependent and therefore, each strain has to be evaluated in order to demonstrate itspotential application in IBD. Lactobacillus jensenii TL2937 attenuates gut acute inflammatoryresponse triggered by Toll-like receptor 4 activation. However, its effect on colitis has not beenevaluated before.Objectives: the aim of this work was to study whether the TL2937 strain was able to protect againstthe development of colitis in a dextran sodium sulfate (DSS)-induced mouse model.Methods: L. jensenii TL2937 was administered to five-week-old female BALB/c mice before (4 days)the induction of colitis, at a dose of 108 cells/mouse/day. Untreated mice were used as controls.Colitis was established by the administration of 2.5% (w/v) DSS in drinking water for 7 days. Micewere examined daily during 18 days for body weight, general appearance, stool consistency, and thepresence of fecal blood. In addition, myeloperoxidase activity and cytokine production wereevaluated in colon tissue samples.Results: Mice fed with L. jensenii TL2937 had lower disease activity index and alterations of colonlength when compared to control mice. In addition, reduced myeloperoxidase activity, lowerproduction of pro-inflammatory (TNF, IL-1, IL-8, MCP-1, IL-15 and IL-17) and higher levels ofimmunoregulatory (IL-10 and IL-27) cytokines were found in TL2937-treated mice. These findingsindicate that L. jensenii TL2937 is able to alleviate DSS-induced colitis and suggest a novel applicationfor this probiotic strain.
Fil: Islam, M. Aminul. Tohoku University; Japón. Bangladesh Agricultural University; Bangladesh
Fil: Garcia Castillo, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad de Concepción; Chile
Fil: Sato, Nana. Tohoku University; Japón
Fil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología; Argentina. Tohoku University; Japón
Fil: Kitazawa, Haruki. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón
8th Congress of European Microbiologists
Glasgow
Reino Unido
Federation of European Microbiological Societies
Society for Applied Microbiology
Materia
IMMUNOBIOTIC
LACTOBACILLUS JENSENI TL2937
COLITIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/215986
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/215986
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Immunobiotic Lactobacillus jenseni TL2937 alleviates dextran sodium sulfate-induced colitis in miceIslam, M. AminulGarcia Castillo, ValeriaSato, NanaAlbarracín, Leonardo MiguelKitazawa, HarukiVillena, Julio CesarIMMUNOBIOTICLACTOBACILLUS JENSENI TL2937COLITIShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Immunobiotics have emerged as promising interventions to alleviate inflammatorydamage in inflammatory bowel disease (IBD). However, the beneficial properties of immunobioticsare strain dependent and therefore, each strain has to be evaluated in order to demonstrate itspotential application in IBD. Lactobacillus jensenii TL2937 attenuates gut acute inflammatoryresponse triggered by Toll-like receptor 4 activation. However, its effect on colitis has not beenevaluated before.Objectives: the aim of this work was to study whether the TL2937 strain was able to protect againstthe development of colitis in a dextran sodium sulfate (DSS)-induced mouse model.Methods: L. jensenii TL2937 was administered to five-week-old female BALB/c mice before (4 days)the induction of colitis, at a dose of 108 cells/mouse/day. Untreated mice were used as controls.Colitis was established by the administration of 2.5% (w/v) DSS in drinking water for 7 days. Micewere examined daily during 18 days for body weight, general appearance, stool consistency, and thepresence of fecal blood. In addition, myeloperoxidase activity and cytokine production wereevaluated in colon tissue samples.Results: Mice fed with L. jensenii TL2937 had lower disease activity index and alterations of colonlength when compared to control mice. In addition, reduced myeloperoxidase activity, lowerproduction of pro-inflammatory (TNF, IL-1, IL-8, MCP-1, IL-15 and IL-17) and higher levels ofimmunoregulatory (IL-10 and IL-27) cytokines were found in TL2937-treated mice. These findingsindicate that L. jensenii TL2937 is able to alleviate DSS-induced colitis and suggest a novel applicationfor this probiotic strain.Fil: Islam, M. Aminul. Tohoku University; Japón. Bangladesh Agricultural University; BangladeshFil: Garcia Castillo, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad de Concepción; ChileFil: Sato, Nana. Tohoku University; JapónFil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología; Argentina. Tohoku University; JapónFil: Kitazawa, Haruki. Tohoku University; JapónFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón8th Congress of European MicrobiologistsGlasgowReino UnidoFederation of European Microbiological SocietiesSociety for Applied MicrobiologyFederation of European Microbiological Societies2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/215986Immunobiotic Lactobacillus jenseni TL2937 alleviates dextran sodium sulfate-induced colitis in mice; 8th Congress of European Microbiologists; Glasgow; Reino Unido; 2019; 979-979CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://fems2019.org/fileadmin/user_upload/FEMS/fems2019_abstractbook.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:02:47Zoai:ri.conicet.gov.ar:11336/215986instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:02:47.8CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Immunobiotic Lactobacillus jenseni TL2937 alleviates dextran sodium sulfate-induced colitis in mice
title Immunobiotic Lactobacillus jenseni TL2937 alleviates dextran sodium sulfate-induced colitis in mice
spellingShingle Immunobiotic Lactobacillus jenseni TL2937 alleviates dextran sodium sulfate-induced colitis in mice
Islam, M. Aminul
IMMUNOBIOTIC
LACTOBACILLUS JENSENI TL2937
COLITIS
title_short Immunobiotic Lactobacillus jenseni TL2937 alleviates dextran sodium sulfate-induced colitis in mice
title_full Immunobiotic Lactobacillus jenseni TL2937 alleviates dextran sodium sulfate-induced colitis in mice
title_fullStr Immunobiotic Lactobacillus jenseni TL2937 alleviates dextran sodium sulfate-induced colitis in mice
title_full_unstemmed Immunobiotic Lactobacillus jenseni TL2937 alleviates dextran sodium sulfate-induced colitis in mice
title_sort Immunobiotic Lactobacillus jenseni TL2937 alleviates dextran sodium sulfate-induced colitis in mice
dc.creator.none.fl_str_mv Islam, M. Aminul
Garcia Castillo, Valeria
Sato, Nana
Albarracín, Leonardo Miguel
Kitazawa, Haruki
Villena, Julio Cesar
author Islam, M. Aminul
author_facet Islam, M. Aminul
Garcia Castillo, Valeria
Sato, Nana
Albarracín, Leonardo Miguel
Kitazawa, Haruki
Villena, Julio Cesar
author_role author
author2 Garcia Castillo, Valeria
Sato, Nana
Albarracín, Leonardo Miguel
Kitazawa, Haruki
Villena, Julio Cesar
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv IMMUNOBIOTIC
LACTOBACILLUS JENSENI TL2937
COLITIS
topic IMMUNOBIOTIC
LACTOBACILLUS JENSENI TL2937
COLITIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Immunobiotics have emerged as promising interventions to alleviate inflammatorydamage in inflammatory bowel disease (IBD). However, the beneficial properties of immunobioticsare strain dependent and therefore, each strain has to be evaluated in order to demonstrate itspotential application in IBD. Lactobacillus jensenii TL2937 attenuates gut acute inflammatoryresponse triggered by Toll-like receptor 4 activation. However, its effect on colitis has not beenevaluated before.Objectives: the aim of this work was to study whether the TL2937 strain was able to protect againstthe development of colitis in a dextran sodium sulfate (DSS)-induced mouse model.Methods: L. jensenii TL2937 was administered to five-week-old female BALB/c mice before (4 days)the induction of colitis, at a dose of 108 cells/mouse/day. Untreated mice were used as controls.Colitis was established by the administration of 2.5% (w/v) DSS in drinking water for 7 days. Micewere examined daily during 18 days for body weight, general appearance, stool consistency, and thepresence of fecal blood. In addition, myeloperoxidase activity and cytokine production wereevaluated in colon tissue samples.Results: Mice fed with L. jensenii TL2937 had lower disease activity index and alterations of colonlength when compared to control mice. In addition, reduced myeloperoxidase activity, lowerproduction of pro-inflammatory (TNF, IL-1, IL-8, MCP-1, IL-15 and IL-17) and higher levels ofimmunoregulatory (IL-10 and IL-27) cytokines were found in TL2937-treated mice. These findingsindicate that L. jensenii TL2937 is able to alleviate DSS-induced colitis and suggest a novel applicationfor this probiotic strain.
Fil: Islam, M. Aminul. Tohoku University; Japón. Bangladesh Agricultural University; Bangladesh
Fil: Garcia Castillo, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad de Concepción; Chile
Fil: Sato, Nana. Tohoku University; Japón
Fil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología; Argentina. Tohoku University; Japón
Fil: Kitazawa, Haruki. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón
8th Congress of European Microbiologists
Glasgow
Reino Unido
Federation of European Microbiological Societies
Society for Applied Microbiology
description Immunobiotics have emerged as promising interventions to alleviate inflammatorydamage in inflammatory bowel disease (IBD). However, the beneficial properties of immunobioticsare strain dependent and therefore, each strain has to be evaluated in order to demonstrate itspotential application in IBD. Lactobacillus jensenii TL2937 attenuates gut acute inflammatoryresponse triggered by Toll-like receptor 4 activation. However, its effect on colitis has not beenevaluated before.Objectives: the aim of this work was to study whether the TL2937 strain was able to protect againstthe development of colitis in a dextran sodium sulfate (DSS)-induced mouse model.Methods: L. jensenii TL2937 was administered to five-week-old female BALB/c mice before (4 days)the induction of colitis, at a dose of 108 cells/mouse/day. Untreated mice were used as controls.Colitis was established by the administration of 2.5% (w/v) DSS in drinking water for 7 days. Micewere examined daily during 18 days for body weight, general appearance, stool consistency, and thepresence of fecal blood. In addition, myeloperoxidase activity and cytokine production wereevaluated in colon tissue samples.Results: Mice fed with L. jensenii TL2937 had lower disease activity index and alterations of colonlength when compared to control mice. In addition, reduced myeloperoxidase activity, lowerproduction of pro-inflammatory (TNF, IL-1, IL-8, MCP-1, IL-15 and IL-17) and higher levels ofimmunoregulatory (IL-10 and IL-27) cytokines were found in TL2937-treated mice. These findingsindicate that L. jensenii TL2937 is able to alleviate DSS-induced colitis and suggest a novel applicationfor this probiotic strain.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/215986
Immunobiotic Lactobacillus jenseni TL2937 alleviates dextran sodium sulfate-induced colitis in mice; 8th Congress of European Microbiologists; Glasgow; Reino Unido; 2019; 979-979
CONICET Digital
CONICET
url http://hdl.handle.net/11336/215986
identifier_str_mv Immunobiotic Lactobacillus jenseni TL2937 alleviates dextran sodium sulfate-induced colitis in mice; 8th Congress of European Microbiologists; Glasgow; Reino Unido; 2019; 979-979
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://fems2019.org/fileadmin/user_upload/FEMS/fems2019_abstractbook.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Federation of European Microbiological Societies
publisher.none.fl_str_mv Federation of European Microbiological Societies
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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