Differential expression of protein in muscle of rats treated with fluoride and exercise

Autores
Lombarte, Mercedes; Fina, Brenda Lorena; Lima, Aline Leite; Lupo, Maela; Rabelo Buzalaf, Marilia Afonso
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Chronic ingestion of fluoride (F) has been reported to produce insulin resistance. Conversely, exercising (E) increases the peripheral response to insulin, enhancing the effect of the hormone on target tissues. Previous work has shown that E can reverse insulin resistance caused by ingesting F. Therefore, the objective of this work was: to study the pattern of protein expression in rats that reversed the insulin resistance induced by F when performing E. Healthy female Sprague-Dawley rats (50 days) were divided into 3 groups (n = 6 per group): Control: drinking water F 0 ppm, F: drinking water F 15ppm and FE: drinking water F 15ppm + E 30 min per day on a treadmill. The treatments were applied for 30 days, then the animals were euthanized and the gastrocnemius muscles were obtained for proteomic analysis. Proteins related to carbohydrate metabolism are less expressed in group F than in the control. In contrast, the decrease in carbohydrate metabolism proteins was not observed in rats treated with F and E. Conclusion: physical exercise reverts the resistance to insulin induced by fluoride counteracting fluoride effects on proteins related to carbohydrate metabolism.
Fil: Lombarte, Mercedes. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Ósea; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Fina, Brenda Lorena. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Ósea; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Lima, Aline Leite. Universidade de Sao Paulo; Brasil
Fil: Lupo, Maela. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Ósea; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Rabelo Buzalaf, Marilia Afonso. Universidade de Sao Paulo; Brasil
Materia
EXERCISE
PROTEINE EXPRESSION
MUSCLE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/156972
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Differential expression of protein in muscle of rats treated with fluoride and exerciseLombarte, MercedesFina, Brenda LorenaLima, Aline LeiteLupo, MaelaRabelo Buzalaf, Marilia AfonsoEXERCISEPROTEINE EXPRESSIONMUSCLEhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Chronic ingestion of fluoride (F) has been reported to produce insulin resistance. Conversely, exercising (E) increases the peripheral response to insulin, enhancing the effect of the hormone on target tissues. Previous work has shown that E can reverse insulin resistance caused by ingesting F. Therefore, the objective of this work was: to study the pattern of protein expression in rats that reversed the insulin resistance induced by F when performing E. Healthy female Sprague-Dawley rats (50 days) were divided into 3 groups (n = 6 per group): Control: drinking water F 0 ppm, F: drinking water F 15ppm and FE: drinking water F 15ppm + E 30 min per day on a treadmill. The treatments were applied for 30 days, then the animals were euthanized and the gastrocnemius muscles were obtained for proteomic analysis. Proteins related to carbohydrate metabolism are less expressed in group F than in the control. In contrast, the decrease in carbohydrate metabolism proteins was not observed in rats treated with F and E. Conclusion: physical exercise reverts the resistance to insulin induced by fluoride counteracting fluoride effects on proteins related to carbohydrate metabolism.Fil: Lombarte, Mercedes. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Ósea; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Fina, Brenda Lorena. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Ósea; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Lima, Aline Leite. Universidade de Sao Paulo; BrasilFil: Lupo, Maela. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Ósea; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Rabelo Buzalaf, Marilia Afonso. Universidade de Sao Paulo; BrasilInternational Society for Fluoride Research2021-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/156972Lombarte, Mercedes; Fina, Brenda Lorena; Lima, Aline Leite; Lupo, Maela; Rabelo Buzalaf, Marilia Afonso; Differential expression of protein in muscle of rats treated with fluoride and exercise; International Society for Fluoride Research; Fluoride; 2021; 8-2021; 1-120015-47252253-4083CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.fluorideresearch.online/info:eu-repo/semantics/altIdentifier/url/https://www.fluorideresearch.online/epub/files/150.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:29:45Zoai:ri.conicet.gov.ar:11336/156972instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:29:45.51CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Differential expression of protein in muscle of rats treated with fluoride and exercise
title Differential expression of protein in muscle of rats treated with fluoride and exercise
spellingShingle Differential expression of protein in muscle of rats treated with fluoride and exercise
Lombarte, Mercedes
EXERCISE
PROTEINE EXPRESSION
MUSCLE
title_short Differential expression of protein in muscle of rats treated with fluoride and exercise
title_full Differential expression of protein in muscle of rats treated with fluoride and exercise
title_fullStr Differential expression of protein in muscle of rats treated with fluoride and exercise
title_full_unstemmed Differential expression of protein in muscle of rats treated with fluoride and exercise
title_sort Differential expression of protein in muscle of rats treated with fluoride and exercise
dc.creator.none.fl_str_mv Lombarte, Mercedes
Fina, Brenda Lorena
Lima, Aline Leite
Lupo, Maela
Rabelo Buzalaf, Marilia Afonso
author Lombarte, Mercedes
author_facet Lombarte, Mercedes
Fina, Brenda Lorena
Lima, Aline Leite
Lupo, Maela
Rabelo Buzalaf, Marilia Afonso
author_role author
author2 Fina, Brenda Lorena
Lima, Aline Leite
Lupo, Maela
Rabelo Buzalaf, Marilia Afonso
author2_role author
author
author
author
dc.subject.none.fl_str_mv EXERCISE
PROTEINE EXPRESSION
MUSCLE
topic EXERCISE
PROTEINE EXPRESSION
MUSCLE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Chronic ingestion of fluoride (F) has been reported to produce insulin resistance. Conversely, exercising (E) increases the peripheral response to insulin, enhancing the effect of the hormone on target tissues. Previous work has shown that E can reverse insulin resistance caused by ingesting F. Therefore, the objective of this work was: to study the pattern of protein expression in rats that reversed the insulin resistance induced by F when performing E. Healthy female Sprague-Dawley rats (50 days) were divided into 3 groups (n = 6 per group): Control: drinking water F 0 ppm, F: drinking water F 15ppm and FE: drinking water F 15ppm + E 30 min per day on a treadmill. The treatments were applied for 30 days, then the animals were euthanized and the gastrocnemius muscles were obtained for proteomic analysis. Proteins related to carbohydrate metabolism are less expressed in group F than in the control. In contrast, the decrease in carbohydrate metabolism proteins was not observed in rats treated with F and E. Conclusion: physical exercise reverts the resistance to insulin induced by fluoride counteracting fluoride effects on proteins related to carbohydrate metabolism.
Fil: Lombarte, Mercedes. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Ósea; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Fina, Brenda Lorena. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Ósea; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Lima, Aline Leite. Universidade de Sao Paulo; Brasil
Fil: Lupo, Maela. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Ósea; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Rabelo Buzalaf, Marilia Afonso. Universidade de Sao Paulo; Brasil
description Chronic ingestion of fluoride (F) has been reported to produce insulin resistance. Conversely, exercising (E) increases the peripheral response to insulin, enhancing the effect of the hormone on target tissues. Previous work has shown that E can reverse insulin resistance caused by ingesting F. Therefore, the objective of this work was: to study the pattern of protein expression in rats that reversed the insulin resistance induced by F when performing E. Healthy female Sprague-Dawley rats (50 days) were divided into 3 groups (n = 6 per group): Control: drinking water F 0 ppm, F: drinking water F 15ppm and FE: drinking water F 15ppm + E 30 min per day on a treadmill. The treatments were applied for 30 days, then the animals were euthanized and the gastrocnemius muscles were obtained for proteomic analysis. Proteins related to carbohydrate metabolism are less expressed in group F than in the control. In contrast, the decrease in carbohydrate metabolism proteins was not observed in rats treated with F and E. Conclusion: physical exercise reverts the resistance to insulin induced by fluoride counteracting fluoride effects on proteins related to carbohydrate metabolism.
publishDate 2021
dc.date.none.fl_str_mv 2021-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/156972
Lombarte, Mercedes; Fina, Brenda Lorena; Lima, Aline Leite; Lupo, Maela; Rabelo Buzalaf, Marilia Afonso; Differential expression of protein in muscle of rats treated with fluoride and exercise; International Society for Fluoride Research; Fluoride; 2021; 8-2021; 1-12
0015-4725
2253-4083
CONICET Digital
CONICET
url http://hdl.handle.net/11336/156972
identifier_str_mv Lombarte, Mercedes; Fina, Brenda Lorena; Lima, Aline Leite; Lupo, Maela; Rabelo Buzalaf, Marilia Afonso; Differential expression of protein in muscle of rats treated with fluoride and exercise; International Society for Fluoride Research; Fluoride; 2021; 8-2021; 1-12
0015-4725
2253-4083
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.fluorideresearch.online/
info:eu-repo/semantics/altIdentifier/url/https://www.fluorideresearch.online/epub/files/150.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv International Society for Fluoride Research
publisher.none.fl_str_mv International Society for Fluoride Research
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082770340675584
score 12.891075

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