Effects of sustained swimming on the red and white muscle transcriptome of rainbow trout (Oncorhynchus mykiss) fed a carbohydrate-rich diet

Autores
Magnoni, Leonardo Julián; Crespo, Diego; Ibarz, Antoni; Blasco, Josefina; Fernández Borrás, Jaume; Planas, Josep V.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Training at sustainable swimming speeds can produce changes in fish skeletal muscle that are important for aquaculture due to their growth-potentiating effects. Such changes may be even more relevant when fish are fed diets containing an increasing proportion of carbohydrates as an energy source. We evaluated the effects of moderate-intensity sustained swimming on the transcriptomic response of red and white muscle in rainbow trout fed a carbohydrate-rich diet using microarray and qPCR. Analysis of the red and white muscle transcriptome in resting or swimming (1.3 body lengths/s) for 30 days revealed significant changes in the expression of a large number of genes (395 and 597, respectively), with a total of 218 differentially expressed genes (DEGs) common for both muscles. A large number of the genes involved in glucose use and energy generation, contraction, development, synthesis and catabolism of proteins were up-regulated in red and white muscle. Additionally, DEGs in both muscles were involved in processes of defense response and apoptosis. Skeletal muscle contraction activates a transcriptional program required for the successful adaptation of both muscles to the changing demands imposed by swimming conditions. Future studies should further clarify the mechanisms involved in the adaptation of both tissues to exercise and assess possible benefits of such conditions for cultured fish.
Fil: Magnoni, Leonardo Julián. Universidad de Barcelona. Facultad de Biología; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Crespo, Diego. Universidad de Barcelona. Facultad de Biología; España
Fil: Ibarz, Antoni. Universidad de Barcelona. Facultad de Biología; España
Fil: Blasco, Josefina. Universidad de Barcelona. Facultad de Biología; España
Fil: Fernández Borrás, Jaume. Universidad de Barcelona. Facultad de Biología; España
Fil: Planas, Josep V.. Universidad de Barcelona. Facultad de Biología; España
Materia
Fish Skeletal Muscle
Moderate-Intensity Endurance Exercise
Transcriptional Regulation
Differential Gene Expression
Glucose Utilization
Glut4
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24277
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/24277
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effects of sustained swimming on the red and white muscle transcriptome of rainbow trout (Oncorhynchus mykiss) fed a carbohydrate-rich dietMagnoni, Leonardo JuliánCrespo, DiegoIbarz, AntoniBlasco, JosefinaFernández Borrás, JaumePlanas, Josep V.Fish Skeletal MuscleModerate-Intensity Endurance ExerciseTranscriptional RegulationDifferential Gene ExpressionGlucose UtilizationGlut4https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Training at sustainable swimming speeds can produce changes in fish skeletal muscle that are important for aquaculture due to their growth-potentiating effects. Such changes may be even more relevant when fish are fed diets containing an increasing proportion of carbohydrates as an energy source. We evaluated the effects of moderate-intensity sustained swimming on the transcriptomic response of red and white muscle in rainbow trout fed a carbohydrate-rich diet using microarray and qPCR. Analysis of the red and white muscle transcriptome in resting or swimming (1.3 body lengths/s) for 30 days revealed significant changes in the expression of a large number of genes (395 and 597, respectively), with a total of 218 differentially expressed genes (DEGs) common for both muscles. A large number of the genes involved in glucose use and energy generation, contraction, development, synthesis and catabolism of proteins were up-regulated in red and white muscle. Additionally, DEGs in both muscles were involved in processes of defense response and apoptosis. Skeletal muscle contraction activates a transcriptional program required for the successful adaptation of both muscles to the changing demands imposed by swimming conditions. Future studies should further clarify the mechanisms involved in the adaptation of both tissues to exercise and assess possible benefits of such conditions for cultured fish.Fil: Magnoni, Leonardo Julián. Universidad de Barcelona. Facultad de Biología; España. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Crespo, Diego. Universidad de Barcelona. Facultad de Biología; EspañaFil: Ibarz, Antoni. Universidad de Barcelona. Facultad de Biología; EspañaFil: Blasco, Josefina. Universidad de Barcelona. Facultad de Biología; EspañaFil: Fernández Borrás, Jaume. Universidad de Barcelona. Facultad de Biología; EspañaFil: Planas, Josep V.. Universidad de Barcelona. Facultad de Biología; EspañaElsevier Science Inc2013-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24277Magnoni, Leonardo Julián; Crespo, Diego; Ibarz, Antoni; Blasco, Josefina; Fernández Borrás, Jaume; et al.; Effects of sustained swimming on the red and white muscle transcriptome of rainbow trout (Oncorhynchus mykiss) fed a carbohydrate-rich diet; Elsevier Science Inc; Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology; 166; 3; 8-2013; 510-5211095-6433CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbpa.2013.08.005info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1095643313002201info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:34Zoai:ri.conicet.gov.ar:11336/24277instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:34.825CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of sustained swimming on the red and white muscle transcriptome of rainbow trout (Oncorhynchus mykiss) fed a carbohydrate-rich diet
title Effects of sustained swimming on the red and white muscle transcriptome of rainbow trout (Oncorhynchus mykiss) fed a carbohydrate-rich diet
spellingShingle Effects of sustained swimming on the red and white muscle transcriptome of rainbow trout (Oncorhynchus mykiss) fed a carbohydrate-rich diet
Magnoni, Leonardo Julián
Fish Skeletal Muscle
Moderate-Intensity Endurance Exercise
Transcriptional Regulation
Differential Gene Expression
Glucose Utilization
Glut4
title_short Effects of sustained swimming on the red and white muscle transcriptome of rainbow trout (Oncorhynchus mykiss) fed a carbohydrate-rich diet
title_full Effects of sustained swimming on the red and white muscle transcriptome of rainbow trout (Oncorhynchus mykiss) fed a carbohydrate-rich diet
title_fullStr Effects of sustained swimming on the red and white muscle transcriptome of rainbow trout (Oncorhynchus mykiss) fed a carbohydrate-rich diet
title_full_unstemmed Effects of sustained swimming on the red and white muscle transcriptome of rainbow trout (Oncorhynchus mykiss) fed a carbohydrate-rich diet
title_sort Effects of sustained swimming on the red and white muscle transcriptome of rainbow trout (Oncorhynchus mykiss) fed a carbohydrate-rich diet
dc.creator.none.fl_str_mv Magnoni, Leonardo Julián
Crespo, Diego
Ibarz, Antoni
Blasco, Josefina
Fernández Borrás, Jaume
Planas, Josep V.
author Magnoni, Leonardo Julián
author_facet Magnoni, Leonardo Julián
Crespo, Diego
Ibarz, Antoni
Blasco, Josefina
Fernández Borrás, Jaume
Planas, Josep V.
author_role author
author2 Crespo, Diego
Ibarz, Antoni
Blasco, Josefina
Fernández Borrás, Jaume
Planas, Josep V.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Fish Skeletal Muscle
Moderate-Intensity Endurance Exercise
Transcriptional Regulation
Differential Gene Expression
Glucose Utilization
Glut4
topic Fish Skeletal Muscle
Moderate-Intensity Endurance Exercise
Transcriptional Regulation
Differential Gene Expression
Glucose Utilization
Glut4
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Training at sustainable swimming speeds can produce changes in fish skeletal muscle that are important for aquaculture due to their growth-potentiating effects. Such changes may be even more relevant when fish are fed diets containing an increasing proportion of carbohydrates as an energy source. We evaluated the effects of moderate-intensity sustained swimming on the transcriptomic response of red and white muscle in rainbow trout fed a carbohydrate-rich diet using microarray and qPCR. Analysis of the red and white muscle transcriptome in resting or swimming (1.3 body lengths/s) for 30 days revealed significant changes in the expression of a large number of genes (395 and 597, respectively), with a total of 218 differentially expressed genes (DEGs) common for both muscles. A large number of the genes involved in glucose use and energy generation, contraction, development, synthesis and catabolism of proteins were up-regulated in red and white muscle. Additionally, DEGs in both muscles were involved in processes of defense response and apoptosis. Skeletal muscle contraction activates a transcriptional program required for the successful adaptation of both muscles to the changing demands imposed by swimming conditions. Future studies should further clarify the mechanisms involved in the adaptation of both tissues to exercise and assess possible benefits of such conditions for cultured fish.
Fil: Magnoni, Leonardo Julián. Universidad de Barcelona. Facultad de Biología; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Crespo, Diego. Universidad de Barcelona. Facultad de Biología; España
Fil: Ibarz, Antoni. Universidad de Barcelona. Facultad de Biología; España
Fil: Blasco, Josefina. Universidad de Barcelona. Facultad de Biología; España
Fil: Fernández Borrás, Jaume. Universidad de Barcelona. Facultad de Biología; España
Fil: Planas, Josep V.. Universidad de Barcelona. Facultad de Biología; España
description Training at sustainable swimming speeds can produce changes in fish skeletal muscle that are important for aquaculture due to their growth-potentiating effects. Such changes may be even more relevant when fish are fed diets containing an increasing proportion of carbohydrates as an energy source. We evaluated the effects of moderate-intensity sustained swimming on the transcriptomic response of red and white muscle in rainbow trout fed a carbohydrate-rich diet using microarray and qPCR. Analysis of the red and white muscle transcriptome in resting or swimming (1.3 body lengths/s) for 30 days revealed significant changes in the expression of a large number of genes (395 and 597, respectively), with a total of 218 differentially expressed genes (DEGs) common for both muscles. A large number of the genes involved in glucose use and energy generation, contraction, development, synthesis and catabolism of proteins were up-regulated in red and white muscle. Additionally, DEGs in both muscles were involved in processes of defense response and apoptosis. Skeletal muscle contraction activates a transcriptional program required for the successful adaptation of both muscles to the changing demands imposed by swimming conditions. Future studies should further clarify the mechanisms involved in the adaptation of both tissues to exercise and assess possible benefits of such conditions for cultured fish.
publishDate 2013
dc.date.none.fl_str_mv 2013-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24277
Magnoni, Leonardo Julián; Crespo, Diego; Ibarz, Antoni; Blasco, Josefina; Fernández Borrás, Jaume; et al.; Effects of sustained swimming on the red and white muscle transcriptome of rainbow trout (Oncorhynchus mykiss) fed a carbohydrate-rich diet; Elsevier Science Inc; Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology; 166; 3; 8-2013; 510-521
1095-6433
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24277
identifier_str_mv Magnoni, Leonardo Julián; Crespo, Diego; Ibarz, Antoni; Blasco, Josefina; Fernández Borrás, Jaume; et al.; Effects of sustained swimming on the red and white muscle transcriptome of rainbow trout (Oncorhynchus mykiss) fed a carbohydrate-rich diet; Elsevier Science Inc; Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology; 166; 3; 8-2013; 510-521
1095-6433
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbpa.2013.08.005
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1095643313002201
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc
publisher.none.fl_str_mv Elsevier Science Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269917549690880
score 13.13397

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