Differential behavioral profile induced by the injection of dipotassium chlorazepate within brain areas that project to the nucleus accumbens septi.

Autores
Llano López, Luis H.; Caif, Fernando; Fraile, Miriam; Tinnirello, Belén; Landa-Gargiulo, Adriana I.; Lafuente, José V.; Baiardi, Gustavo Carlos; Gargiulo, Pascual Angel
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The effect of the agonism on g-aminobutyric acid (GABA) receptors was studied within medial prefrontal cortex (mPFC), amygdala (AMY) and ventral hipocampus (VH) in the plus-maze test in male rats bilaterally cannulated. These structures send glutamatergic projections to the nucleus accumbens septi (NAS), in which interaction and integration between these afferent pathways has been described. In a previous study of our group, blockade of glutamatergic transmission within NAS induced an anxiolytic like effect. Methods: Three rat groups received either saline or dipotassium chlorazepate (1 or 2 μg/1 μl solution) 15 min before testing. Time spent in the open arms (TSOA), time per entry (TPE), extreme arrivals (EA), open and closed arms entries (OAE, CAE) and relation- ship between open- and closed-arms quotient (OCAQ) were recorded. Results: In the AMY injected group TSOA, OAE and EA were increased by the higher doses of dipotassium chlorazepate (p < 0.01). In the mPFC, TPE was decreased by both doses (p < 0.05). Injection within ventral hippocampus (VH) decreased TSOA, OAE and OCAQ with lower doses (p < 0.05). When the three studied saline groups were compared, TSOA, OAE, EA and OCAQ were en- hanced in the VH group when compared to mPFC and AMY (p < 0.001). Insertion of inner canula (p < 0.001, p < 0.01, p < 0.01) and saline injection showed an increasing significant difference (p < 0.001 in all cases) with the action of guide cannula alone within VH in TSOA, OAE and EA. Conclusion: We conclude that the injection of dipotassium chlorazepate has a differential effect depending of the brain area, leading to facilitatory and inhibitory effects on anxiety processing.
Fil: Llano López, Luis H.. Universidad Nacional de Cuyo; Argentina
Fil: Caif, Fernando. Universidad Nacional de Cuyo; Argentina
Fil: Fraile, Miriam. Universidad Nacional de Cuyo; Argentina
Fil: Tinnirello, Belén. Universidad Nacional de Cuyo; Argentina
Fil: Landa-Gargiulo, Adriana I.. Universidad Nacional de Cuyo; Argentina
Fil: Lafuente, José V.. Universidad del Pais Vasco. Departamento de Neurociencias; España;
Fil: Baiardi, Gustavo Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Gargiulo, Pascual Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Materia
GABA
AMYGDALA
MEDIAL PREFRONTAL CORTEX
SCHIZOPHRENIA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/3120
Acceder
id CONICETDig_0b67c6054dfa2b6d918881130357ce16
oai_identifier_str oai:ri.conicet.gov.ar:11336/3120
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Differential behavioral profile induced by the injection of dipotassium chlorazepate within brain areas that project to the nucleus accumbens septi.Llano López, Luis H.Caif, FernandoFraile, MiriamTinnirello, BelénLanda-Gargiulo, Adriana I.Lafuente, José V.Baiardi, Gustavo CarlosGargiulo, Pascual AngelGABAAMYGDALAMEDIAL PREFRONTAL CORTEXSCHIZOPHRENIAhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The effect of the agonism on g-aminobutyric acid (GABA) receptors was studied within medial prefrontal cortex (mPFC), amygdala (AMY) and ventral hipocampus (VH) in the plus-maze test in male rats bilaterally cannulated. These structures send glutamatergic projections to the nucleus accumbens septi (NAS), in which interaction and integration between these afferent pathways has been described. In a previous study of our group, blockade of glutamatergic transmission within NAS induced an anxiolytic like effect. Methods: Three rat groups received either saline or dipotassium chlorazepate (1 or 2 μg/1 μl solution) 15 min before testing. Time spent in the open arms (TSOA), time per entry (TPE), extreme arrivals (EA), open and closed arms entries (OAE, CAE) and relation- ship between open- and closed-arms quotient (OCAQ) were recorded. Results: In the AMY injected group TSOA, OAE and EA were increased by the higher doses of dipotassium chlorazepate (p < 0.01). In the mPFC, TPE was decreased by both doses (p < 0.05). Injection within ventral hippocampus (VH) decreased TSOA, OAE and OCAQ with lower doses (p < 0.05). When the three studied saline groups were compared, TSOA, OAE, EA and OCAQ were en- hanced in the VH group when compared to mPFC and AMY (p < 0.001). Insertion of inner canula (p < 0.001, p < 0.01, p < 0.01) and saline injection showed an increasing significant difference (p < 0.001 in all cases) with the action of guide cannula alone within VH in TSOA, OAE and EA. Conclusion: We conclude that the injection of dipotassium chlorazepate has a differential effect depending of the brain area, leading to facilitatory and inhibitory effects on anxiety processing.Fil: Llano López, Luis H.. Universidad Nacional de Cuyo; ArgentinaFil: Caif, Fernando. Universidad Nacional de Cuyo; ArgentinaFil: Fraile, Miriam. Universidad Nacional de Cuyo; ArgentinaFil: Tinnirello, Belén. Universidad Nacional de Cuyo; ArgentinaFil: Landa-Gargiulo, Adriana I.. Universidad Nacional de Cuyo; ArgentinaFil: Lafuente, José V.. Universidad del Pais Vasco. Departamento de Neurociencias; España;Fil: Baiardi, Gustavo Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Gargiulo, Pascual Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaPolish Acad Sciences Inst Pharmacology2013-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/3120Llano López, Luis H.; Caif, Fernando; Fraile, Miriam; Tinnirello, Belén; Landa-Gargiulo, Adriana I.; et al.; Differential behavioral profile induced by the injection of dipotassium chlorazepate within brain areas that project to the nucleus accumbens septi.; Polish Acad Sciences Inst Pharmacology; Pharmacological Reports; 65; 8-2013; 566-5781734-1140enginfo:eu-repo/semantics/altIdentifier/url/http://www.if-pan.krakow.pl/pjp/pdf/2013/3_566.pdfinfo:eu-repo/semantics/altIdentifier/pmid/23950579info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:57:32Zoai:ri.conicet.gov.ar:11336/3120instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:57:32.693CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Differential behavioral profile induced by the injection of dipotassium chlorazepate within brain areas that project to the nucleus accumbens septi.
title Differential behavioral profile induced by the injection of dipotassium chlorazepate within brain areas that project to the nucleus accumbens septi.
spellingShingle Differential behavioral profile induced by the injection of dipotassium chlorazepate within brain areas that project to the nucleus accumbens septi.
Llano López, Luis H.
GABA
AMYGDALA
MEDIAL PREFRONTAL CORTEX
SCHIZOPHRENIA
title_short Differential behavioral profile induced by the injection of dipotassium chlorazepate within brain areas that project to the nucleus accumbens septi.
title_full Differential behavioral profile induced by the injection of dipotassium chlorazepate within brain areas that project to the nucleus accumbens septi.
title_fullStr Differential behavioral profile induced by the injection of dipotassium chlorazepate within brain areas that project to the nucleus accumbens septi.
title_full_unstemmed Differential behavioral profile induced by the injection of dipotassium chlorazepate within brain areas that project to the nucleus accumbens septi.
title_sort Differential behavioral profile induced by the injection of dipotassium chlorazepate within brain areas that project to the nucleus accumbens septi.
dc.creator.none.fl_str_mv Llano López, Luis H.
Caif, Fernando
Fraile, Miriam
Tinnirello, Belén
Landa-Gargiulo, Adriana I.
Lafuente, José V.
Baiardi, Gustavo Carlos
Gargiulo, Pascual Angel
author Llano López, Luis H.
author_facet Llano López, Luis H.
Caif, Fernando
Fraile, Miriam
Tinnirello, Belén
Landa-Gargiulo, Adriana I.
Lafuente, José V.
Baiardi, Gustavo Carlos
Gargiulo, Pascual Angel
author_role author
author2 Caif, Fernando
Fraile, Miriam
Tinnirello, Belén
Landa-Gargiulo, Adriana I.
Lafuente, José V.
Baiardi, Gustavo Carlos
Gargiulo, Pascual Angel
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv GABA
AMYGDALA
MEDIAL PREFRONTAL CORTEX
SCHIZOPHRENIA
topic GABA
AMYGDALA
MEDIAL PREFRONTAL CORTEX
SCHIZOPHRENIA
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The effect of the agonism on g-aminobutyric acid (GABA) receptors was studied within medial prefrontal cortex (mPFC), amygdala (AMY) and ventral hipocampus (VH) in the plus-maze test in male rats bilaterally cannulated. These structures send glutamatergic projections to the nucleus accumbens septi (NAS), in which interaction and integration between these afferent pathways has been described. In a previous study of our group, blockade of glutamatergic transmission within NAS induced an anxiolytic like effect. Methods: Three rat groups received either saline or dipotassium chlorazepate (1 or 2 μg/1 μl solution) 15 min before testing. Time spent in the open arms (TSOA), time per entry (TPE), extreme arrivals (EA), open and closed arms entries (OAE, CAE) and relation- ship between open- and closed-arms quotient (OCAQ) were recorded. Results: In the AMY injected group TSOA, OAE and EA were increased by the higher doses of dipotassium chlorazepate (p < 0.01). In the mPFC, TPE was decreased by both doses (p < 0.05). Injection within ventral hippocampus (VH) decreased TSOA, OAE and OCAQ with lower doses (p < 0.05). When the three studied saline groups were compared, TSOA, OAE, EA and OCAQ were en- hanced in the VH group when compared to mPFC and AMY (p < 0.001). Insertion of inner canula (p < 0.001, p < 0.01, p < 0.01) and saline injection showed an increasing significant difference (p < 0.001 in all cases) with the action of guide cannula alone within VH in TSOA, OAE and EA. Conclusion: We conclude that the injection of dipotassium chlorazepate has a differential effect depending of the brain area, leading to facilitatory and inhibitory effects on anxiety processing.
Fil: Llano López, Luis H.. Universidad Nacional de Cuyo; Argentina
Fil: Caif, Fernando. Universidad Nacional de Cuyo; Argentina
Fil: Fraile, Miriam. Universidad Nacional de Cuyo; Argentina
Fil: Tinnirello, Belén. Universidad Nacional de Cuyo; Argentina
Fil: Landa-Gargiulo, Adriana I.. Universidad Nacional de Cuyo; Argentina
Fil: Lafuente, José V.. Universidad del Pais Vasco. Departamento de Neurociencias; España;
Fil: Baiardi, Gustavo Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Gargiulo, Pascual Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
description The effect of the agonism on g-aminobutyric acid (GABA) receptors was studied within medial prefrontal cortex (mPFC), amygdala (AMY) and ventral hipocampus (VH) in the plus-maze test in male rats bilaterally cannulated. These structures send glutamatergic projections to the nucleus accumbens septi (NAS), in which interaction and integration between these afferent pathways has been described. In a previous study of our group, blockade of glutamatergic transmission within NAS induced an anxiolytic like effect. Methods: Three rat groups received either saline or dipotassium chlorazepate (1 or 2 μg/1 μl solution) 15 min before testing. Time spent in the open arms (TSOA), time per entry (TPE), extreme arrivals (EA), open and closed arms entries (OAE, CAE) and relation- ship between open- and closed-arms quotient (OCAQ) were recorded. Results: In the AMY injected group TSOA, OAE and EA were increased by the higher doses of dipotassium chlorazepate (p < 0.01). In the mPFC, TPE was decreased by both doses (p < 0.05). Injection within ventral hippocampus (VH) decreased TSOA, OAE and OCAQ with lower doses (p < 0.05). When the three studied saline groups were compared, TSOA, OAE, EA and OCAQ were en- hanced in the VH group when compared to mPFC and AMY (p < 0.001). Insertion of inner canula (p < 0.001, p < 0.01, p < 0.01) and saline injection showed an increasing significant difference (p < 0.001 in all cases) with the action of guide cannula alone within VH in TSOA, OAE and EA. Conclusion: We conclude that the injection of dipotassium chlorazepate has a differential effect depending of the brain area, leading to facilitatory and inhibitory effects on anxiety processing.
publishDate 2013
dc.date.none.fl_str_mv 2013-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/3120
Llano López, Luis H.; Caif, Fernando; Fraile, Miriam; Tinnirello, Belén; Landa-Gargiulo, Adriana I.; et al.; Differential behavioral profile induced by the injection of dipotassium chlorazepate within brain areas that project to the nucleus accumbens septi.; Polish Acad Sciences Inst Pharmacology; Pharmacological Reports; 65; 8-2013; 566-578
1734-1140
url http://hdl.handle.net/11336/3120
identifier_str_mv Llano López, Luis H.; Caif, Fernando; Fraile, Miriam; Tinnirello, Belén; Landa-Gargiulo, Adriana I.; et al.; Differential behavioral profile induced by the injection of dipotassium chlorazepate within brain areas that project to the nucleus accumbens septi.; Polish Acad Sciences Inst Pharmacology; Pharmacological Reports; 65; 8-2013; 566-578
1734-1140
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.if-pan.krakow.pl/pjp/pdf/2013/3_566.pdf
info:eu-repo/semantics/altIdentifier/pmid/23950579
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Polish Acad Sciences Inst Pharmacology
publisher.none.fl_str_mv Polish Acad Sciences Inst Pharmacology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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