Evaluation of the antibacterial effects of vancomycin hydrochloride released from agar-gelatin-bioactive glass composites

Autores
Rivadeneira, Josefina; Di Virgilio, Ana Laura; Audisio, Marcela Carina; Boccaccini, Aldo R.; Gorustovich Alonso, Alejandro Adrian
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this work was to evaluate the perfomance of agar-gelatin (AG) composites and AG-containing 45S5 bioactive glass (BG) microparticles (AGBG) in relation to their water uptake capacity, sustained release of a drug over time, and antibacterial effects. The composites were fabricated by the gel-casting method. To impart the local drug release capacity, vancomycin hydrochloride (VC) was loaded in the composites in concentrations of 0.5 and 1 mg ml(-1). VC release was assessed in distilled water at 37 °C up to 72 h and quantified spectrophotometrically. The antibacterial activity of composites was evaluated by the inhibition zone test and the plate count method. The experiments were performed in vitro up to 48 h on three staphylococcus strains: Staphylococcus aureus ATCC29213, S. aureus ATCC6538 and Staphylococcus epidermidis ATCC12228. The results showed that the addition of BG to AG composites did not affect the degree of water uptake. The release of VC was significantly affected by the presence of BG. VC release was higher from AGBGVC films than from AGVC ones over prolonged incubation times. Bacterial inhibition zones were found around the composites. The halos were larger when the cells were put in contact with AGVC composites than when they were put in contact with AGBGVC ones. Nevertheless, the viable count method demonstrated that the composites inhibited Staphylococcus cell growth with no statistical differences. In conclusion, the addition of BG did not reflect an improvement in the parameters studied. On the other hand, composites loaded with VC would have a role in prophylaxis against bacterial infection.
Fil: Rivadeneira, Josefina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingenieria "Hilario Fernandez Long". Grupo Vinculado al Intecin - Grupo Interdisciplinario en Materiales; Argentina
Fil: Di Virgilio, Ana Laura. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Audisio, Marcela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química (i); Argentina
Fil: Boccaccini, Aldo R.. Universitat Erlangen-Nuremberg; Alemania
Fil: Gorustovich Alonso, Alejandro Adrian. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingenieria "Hilario Fernandez Long". Grupo Vinculado al Intecin - Grupo Interdisciplinario en Materiales; Argentina
Materia
Biopolymers
Bioactive Glass
Vancomycin
Staphylococci
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7117
Acceder
id CONICETDig_09fd84e3c39af42f97c771638807e46b
oai_identifier_str oai:ri.conicet.gov.ar:11336/7117
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of the antibacterial effects of vancomycin hydrochloride released from agar-gelatin-bioactive glass compositesRivadeneira, JosefinaDi Virgilio, Ana LauraAudisio, Marcela CarinaBoccaccini, Aldo R.Gorustovich Alonso, Alejandro AdrianBiopolymersBioactive GlassVancomycinStaphylococcihttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3The aim of this work was to evaluate the perfomance of agar-gelatin (AG) composites and AG-containing 45S5 bioactive glass (BG) microparticles (AGBG) in relation to their water uptake capacity, sustained release of a drug over time, and antibacterial effects. The composites were fabricated by the gel-casting method. To impart the local drug release capacity, vancomycin hydrochloride (VC) was loaded in the composites in concentrations of 0.5 and 1 mg ml(-1). VC release was assessed in distilled water at 37 °C up to 72 h and quantified spectrophotometrically. The antibacterial activity of composites was evaluated by the inhibition zone test and the plate count method. The experiments were performed in vitro up to 48 h on three staphylococcus strains: Staphylococcus aureus ATCC29213, S. aureus ATCC6538 and Staphylococcus epidermidis ATCC12228. The results showed that the addition of BG to AG composites did not affect the degree of water uptake. The release of VC was significantly affected by the presence of BG. VC release was higher from AGBGVC films than from AGVC ones over prolonged incubation times. Bacterial inhibition zones were found around the composites. The halos were larger when the cells were put in contact with AGVC composites than when they were put in contact with AGBGVC ones. Nevertheless, the viable count method demonstrated that the composites inhibited Staphylococcus cell growth with no statistical differences. In conclusion, the addition of BG did not reflect an improvement in the parameters studied. On the other hand, composites loaded with VC would have a role in prophylaxis against bacterial infection.Fil: Rivadeneira, Josefina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingenieria "Hilario Fernandez Long". Grupo Vinculado al Intecin - Grupo Interdisciplinario en Materiales; ArgentinaFil: Di Virgilio, Ana Laura. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; ArgentinaFil: Audisio, Marcela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química (i); ArgentinaFil: Boccaccini, Aldo R.. Universitat Erlangen-Nuremberg; AlemaniaFil: Gorustovich Alonso, Alejandro Adrian. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingenieria "Hilario Fernandez Long". Grupo Vinculado al Intecin - Grupo Interdisciplinario en Materiales; ArgentinaIOP Publishing2015-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7117Rivadeneira, Josefina; Di Virgilio, Ana Laura; Audisio, Marcela Carina; Boccaccini, Aldo R.; Gorustovich Alonso, Alejandro Adrian; Evaluation of the antibacterial effects of vancomycin hydrochloride released from agar-gelatin-bioactive glass composites; IOP Publishing; Biomedical Materials; 10; 1; 2-2015; 15011-150111748-6041enginfo:eu-repo/semantics/altIdentifier/url/http://iopscience.iop.org/article/10.1088/1748-6041/10/1/015011info:eu-repo/semantics/altIdentifier/doi/10.1088/1748-6041/10/1/015011info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:17:53Zoai:ri.conicet.gov.ar:11336/7117instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:17:53.907CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of the antibacterial effects of vancomycin hydrochloride released from agar-gelatin-bioactive glass composites
title Evaluation of the antibacterial effects of vancomycin hydrochloride released from agar-gelatin-bioactive glass composites
spellingShingle Evaluation of the antibacterial effects of vancomycin hydrochloride released from agar-gelatin-bioactive glass composites
Rivadeneira, Josefina
Biopolymers
Bioactive Glass
Vancomycin
Staphylococci
title_short Evaluation of the antibacterial effects of vancomycin hydrochloride released from agar-gelatin-bioactive glass composites
title_full Evaluation of the antibacterial effects of vancomycin hydrochloride released from agar-gelatin-bioactive glass composites
title_fullStr Evaluation of the antibacterial effects of vancomycin hydrochloride released from agar-gelatin-bioactive glass composites
title_full_unstemmed Evaluation of the antibacterial effects of vancomycin hydrochloride released from agar-gelatin-bioactive glass composites
title_sort Evaluation of the antibacterial effects of vancomycin hydrochloride released from agar-gelatin-bioactive glass composites
dc.creator.none.fl_str_mv Rivadeneira, Josefina
Di Virgilio, Ana Laura
Audisio, Marcela Carina
Boccaccini, Aldo R.
Gorustovich Alonso, Alejandro Adrian
author Rivadeneira, Josefina
author_facet Rivadeneira, Josefina
Di Virgilio, Ana Laura
Audisio, Marcela Carina
Boccaccini, Aldo R.
Gorustovich Alonso, Alejandro Adrian
author_role author
author2 Di Virgilio, Ana Laura
Audisio, Marcela Carina
Boccaccini, Aldo R.
Gorustovich Alonso, Alejandro Adrian
author2_role author
author
author
author
dc.subject.none.fl_str_mv Biopolymers
Bioactive Glass
Vancomycin
Staphylococci
topic Biopolymers
Bioactive Glass
Vancomycin
Staphylococci
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The aim of this work was to evaluate the perfomance of agar-gelatin (AG) composites and AG-containing 45S5 bioactive glass (BG) microparticles (AGBG) in relation to their water uptake capacity, sustained release of a drug over time, and antibacterial effects. The composites were fabricated by the gel-casting method. To impart the local drug release capacity, vancomycin hydrochloride (VC) was loaded in the composites in concentrations of 0.5 and 1 mg ml(-1). VC release was assessed in distilled water at 37 °C up to 72 h and quantified spectrophotometrically. The antibacterial activity of composites was evaluated by the inhibition zone test and the plate count method. The experiments were performed in vitro up to 48 h on three staphylococcus strains: Staphylococcus aureus ATCC29213, S. aureus ATCC6538 and Staphylococcus epidermidis ATCC12228. The results showed that the addition of BG to AG composites did not affect the degree of water uptake. The release of VC was significantly affected by the presence of BG. VC release was higher from AGBGVC films than from AGVC ones over prolonged incubation times. Bacterial inhibition zones were found around the composites. The halos were larger when the cells were put in contact with AGVC composites than when they were put in contact with AGBGVC ones. Nevertheless, the viable count method demonstrated that the composites inhibited Staphylococcus cell growth with no statistical differences. In conclusion, the addition of BG did not reflect an improvement in the parameters studied. On the other hand, composites loaded with VC would have a role in prophylaxis against bacterial infection.
Fil: Rivadeneira, Josefina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingenieria "Hilario Fernandez Long". Grupo Vinculado al Intecin - Grupo Interdisciplinario en Materiales; Argentina
Fil: Di Virgilio, Ana Laura. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Audisio, Marcela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química (i); Argentina
Fil: Boccaccini, Aldo R.. Universitat Erlangen-Nuremberg; Alemania
Fil: Gorustovich Alonso, Alejandro Adrian. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingenieria "Hilario Fernandez Long". Grupo Vinculado al Intecin - Grupo Interdisciplinario en Materiales; Argentina
description The aim of this work was to evaluate the perfomance of agar-gelatin (AG) composites and AG-containing 45S5 bioactive glass (BG) microparticles (AGBG) in relation to their water uptake capacity, sustained release of a drug over time, and antibacterial effects. The composites were fabricated by the gel-casting method. To impart the local drug release capacity, vancomycin hydrochloride (VC) was loaded in the composites in concentrations of 0.5 and 1 mg ml(-1). VC release was assessed in distilled water at 37 °C up to 72 h and quantified spectrophotometrically. The antibacterial activity of composites was evaluated by the inhibition zone test and the plate count method. The experiments were performed in vitro up to 48 h on three staphylococcus strains: Staphylococcus aureus ATCC29213, S. aureus ATCC6538 and Staphylococcus epidermidis ATCC12228. The results showed that the addition of BG to AG composites did not affect the degree of water uptake. The release of VC was significantly affected by the presence of BG. VC release was higher from AGBGVC films than from AGVC ones over prolonged incubation times. Bacterial inhibition zones were found around the composites. The halos were larger when the cells were put in contact with AGVC composites than when they were put in contact with AGBGVC ones. Nevertheless, the viable count method demonstrated that the composites inhibited Staphylococcus cell growth with no statistical differences. In conclusion, the addition of BG did not reflect an improvement in the parameters studied. On the other hand, composites loaded with VC would have a role in prophylaxis against bacterial infection.
publishDate 2015
dc.date.none.fl_str_mv 2015-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7117
Rivadeneira, Josefina; Di Virgilio, Ana Laura; Audisio, Marcela Carina; Boccaccini, Aldo R.; Gorustovich Alonso, Alejandro Adrian; Evaluation of the antibacterial effects of vancomycin hydrochloride released from agar-gelatin-bioactive glass composites; IOP Publishing; Biomedical Materials; 10; 1; 2-2015; 15011-15011
1748-6041
url http://hdl.handle.net/11336/7117
identifier_str_mv Rivadeneira, Josefina; Di Virgilio, Ana Laura; Audisio, Marcela Carina; Boccaccini, Aldo R.; Gorustovich Alonso, Alejandro Adrian; Evaluation of the antibacterial effects of vancomycin hydrochloride released from agar-gelatin-bioactive glass composites; IOP Publishing; Biomedical Materials; 10; 1; 2-2015; 15011-15011
1748-6041
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://iopscience.iop.org/article/10.1088/1748-6041/10/1/015011
info:eu-repo/semantics/altIdentifier/doi/10.1088/1748-6041/10/1/015011
info:eu-repo/semantics/altIdentifier/doi/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv IOP Publishing
publisher.none.fl_str_mv IOP Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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