Physicochemical characterization and cytotoxic studies of nonionic surfactant vesicles using sucrose esters as oral delivery systems
- Autores
- Valdés, Karina; Morilla, María José; Romero, Eder Lilia; Chávez, Jorge
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Several nanotechnological solutions for mucosal immunization have been proposed, such as nanoparticles, liposomes, solid lipidic particles, micelles, and surfactant vesicles. In recent years, surfactant vesicles have gained increasing scientific attention as an alternative potential drug delivery system to the conventional liposome. This type of vesicle known as niosomes or nonionic surfactant vesicles (NSVs) has a structure and properties similar to those of liposomes. Both of them can transport hydrophilic drugs by encapsulation in the aqueous inner pool or hydrophobic drugs by intercalation into hydrophobic domains. The aim of this study was to prepare and characterize vesicles formed by sucrose esters as protective systems of bioactive molecules for oral administration. Vesicles were prepared using two commercial products formed by mixtures of mono and diesters S-570 and S-770, respectively. Determined parameters were size and zeta potential; the stability of formulations was tested in presence of increasing concentrations of a surfactant, and at several pH values observed in the gastrointestinal tract. Solubilization experiences showed an initial decrease in size for vesicles of both ester mixtures, samples showed detergent resistance at higher Triton X-100 concentrations. Vesicles showed stability at pH 5–7.4 up to 90 min; however, both formulations showed colloidal instability at pH = 2, which corresponds to the isoelectric point of these vesicles. To evaluate the cytotoxicity of both vesicle formulations and separately each pure ester, Caco-2 cells were used. Cytotoxic evaluation indicated that both types of vesicles and free sucrose distearate were safe for Caco-2 viability; however, free sucrose monostearate was toxic for the cells. As a conclusion of these preliminary studies, it can be stated that vesicles formed with mixtures of sucrose esters showed a size in the range of 200 nm maintaining their size when exposed to the action of a surfactant, but showing aggregation at acidic pH.
Fil: Valdés, Karina. Universidad de Chile; Chile
Fil: Morilla, María José. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Romero, Eder Lilia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chávez, Jorge. Universidad de Chile; Chile - Materia
-
Nonionic Surfactant Vesicles
Sucrose Stearate
Cytotoxicity
Stability - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/32819
Ver los metadatos del registro completo
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Physicochemical characterization and cytotoxic studies of nonionic surfactant vesicles using sucrose esters as oral delivery systemsValdés, KarinaMorilla, María JoséRomero, Eder LiliaChávez, JorgeNonionic Surfactant VesiclesSucrose StearateCytotoxicityStabilityhttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Several nanotechnological solutions for mucosal immunization have been proposed, such as nanoparticles, liposomes, solid lipidic particles, micelles, and surfactant vesicles. In recent years, surfactant vesicles have gained increasing scientific attention as an alternative potential drug delivery system to the conventional liposome. This type of vesicle known as niosomes or nonionic surfactant vesicles (NSVs) has a structure and properties similar to those of liposomes. Both of them can transport hydrophilic drugs by encapsulation in the aqueous inner pool or hydrophobic drugs by intercalation into hydrophobic domains. The aim of this study was to prepare and characterize vesicles formed by sucrose esters as protective systems of bioactive molecules for oral administration. Vesicles were prepared using two commercial products formed by mixtures of mono and diesters S-570 and S-770, respectively. Determined parameters were size and zeta potential; the stability of formulations was tested in presence of increasing concentrations of a surfactant, and at several pH values observed in the gastrointestinal tract. Solubilization experiences showed an initial decrease in size for vesicles of both ester mixtures, samples showed detergent resistance at higher Triton X-100 concentrations. Vesicles showed stability at pH 5–7.4 up to 90 min; however, both formulations showed colloidal instability at pH = 2, which corresponds to the isoelectric point of these vesicles. To evaluate the cytotoxicity of both vesicle formulations and separately each pure ester, Caco-2 cells were used. Cytotoxic evaluation indicated that both types of vesicles and free sucrose distearate were safe for Caco-2 viability; however, free sucrose monostearate was toxic for the cells. As a conclusion of these preliminary studies, it can be stated that vesicles formed with mixtures of sucrose esters showed a size in the range of 200 nm maintaining their size when exposed to the action of a surfactant, but showing aggregation at acidic pH.Fil: Valdés, Karina. Universidad de Chile; ChileFil: Morilla, María José. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Romero, Eder Lilia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Chávez, Jorge. Universidad de Chile; ChileElsevier2014-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/32819Chávez, Jorge; Romero, Eder Lilia; Morilla, María José; Valdés, Karina; Physicochemical characterization and cytotoxic studies of nonionic surfactant vesicles using sucrose esters as oral delivery systems; Elsevier; Colloids and Surfaces B: Biointerfaces; 117; 2-2014; 1-60927-7765CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0927776514000307info:eu-repo/semantics/altIdentifier/doi/10.1016/j.colsurfb.2014.01.029info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:46:50Zoai:ri.conicet.gov.ar:11336/32819instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:46:50.741CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Physicochemical characterization and cytotoxic studies of nonionic surfactant vesicles using sucrose esters as oral delivery systems |
title |
Physicochemical characterization and cytotoxic studies of nonionic surfactant vesicles using sucrose esters as oral delivery systems |
spellingShingle |
Physicochemical characterization and cytotoxic studies of nonionic surfactant vesicles using sucrose esters as oral delivery systems Valdés, Karina Nonionic Surfactant Vesicles Sucrose Stearate Cytotoxicity Stability |
title_short |
Physicochemical characterization and cytotoxic studies of nonionic surfactant vesicles using sucrose esters as oral delivery systems |
title_full |
Physicochemical characterization and cytotoxic studies of nonionic surfactant vesicles using sucrose esters as oral delivery systems |
title_fullStr |
Physicochemical characterization and cytotoxic studies of nonionic surfactant vesicles using sucrose esters as oral delivery systems |
title_full_unstemmed |
Physicochemical characterization and cytotoxic studies of nonionic surfactant vesicles using sucrose esters as oral delivery systems |
title_sort |
Physicochemical characterization and cytotoxic studies of nonionic surfactant vesicles using sucrose esters as oral delivery systems |
dc.creator.none.fl_str_mv |
Valdés, Karina Morilla, María José Romero, Eder Lilia Chávez, Jorge |
author |
Valdés, Karina |
author_facet |
Valdés, Karina Morilla, María José Romero, Eder Lilia Chávez, Jorge |
author_role |
author |
author2 |
Morilla, María José Romero, Eder Lilia Chávez, Jorge |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Nonionic Surfactant Vesicles Sucrose Stearate Cytotoxicity Stability |
topic |
Nonionic Surfactant Vesicles Sucrose Stearate Cytotoxicity Stability |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.10 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
Several nanotechnological solutions for mucosal immunization have been proposed, such as nanoparticles, liposomes, solid lipidic particles, micelles, and surfactant vesicles. In recent years, surfactant vesicles have gained increasing scientific attention as an alternative potential drug delivery system to the conventional liposome. This type of vesicle known as niosomes or nonionic surfactant vesicles (NSVs) has a structure and properties similar to those of liposomes. Both of them can transport hydrophilic drugs by encapsulation in the aqueous inner pool or hydrophobic drugs by intercalation into hydrophobic domains. The aim of this study was to prepare and characterize vesicles formed by sucrose esters as protective systems of bioactive molecules for oral administration. Vesicles were prepared using two commercial products formed by mixtures of mono and diesters S-570 and S-770, respectively. Determined parameters were size and zeta potential; the stability of formulations was tested in presence of increasing concentrations of a surfactant, and at several pH values observed in the gastrointestinal tract. Solubilization experiences showed an initial decrease in size for vesicles of both ester mixtures, samples showed detergent resistance at higher Triton X-100 concentrations. Vesicles showed stability at pH 5–7.4 up to 90 min; however, both formulations showed colloidal instability at pH = 2, which corresponds to the isoelectric point of these vesicles. To evaluate the cytotoxicity of both vesicle formulations and separately each pure ester, Caco-2 cells were used. Cytotoxic evaluation indicated that both types of vesicles and free sucrose distearate were safe for Caco-2 viability; however, free sucrose monostearate was toxic for the cells. As a conclusion of these preliminary studies, it can be stated that vesicles formed with mixtures of sucrose esters showed a size in the range of 200 nm maintaining their size when exposed to the action of a surfactant, but showing aggregation at acidic pH. Fil: Valdés, Karina. Universidad de Chile; Chile Fil: Morilla, María José. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Romero, Eder Lilia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Chávez, Jorge. Universidad de Chile; Chile |
description |
Several nanotechnological solutions for mucosal immunization have been proposed, such as nanoparticles, liposomes, solid lipidic particles, micelles, and surfactant vesicles. In recent years, surfactant vesicles have gained increasing scientific attention as an alternative potential drug delivery system to the conventional liposome. This type of vesicle known as niosomes or nonionic surfactant vesicles (NSVs) has a structure and properties similar to those of liposomes. Both of them can transport hydrophilic drugs by encapsulation in the aqueous inner pool or hydrophobic drugs by intercalation into hydrophobic domains. The aim of this study was to prepare and characterize vesicles formed by sucrose esters as protective systems of bioactive molecules for oral administration. Vesicles were prepared using two commercial products formed by mixtures of mono and diesters S-570 and S-770, respectively. Determined parameters were size and zeta potential; the stability of formulations was tested in presence of increasing concentrations of a surfactant, and at several pH values observed in the gastrointestinal tract. Solubilization experiences showed an initial decrease in size for vesicles of both ester mixtures, samples showed detergent resistance at higher Triton X-100 concentrations. Vesicles showed stability at pH 5–7.4 up to 90 min; however, both formulations showed colloidal instability at pH = 2, which corresponds to the isoelectric point of these vesicles. To evaluate the cytotoxicity of both vesicle formulations and separately each pure ester, Caco-2 cells were used. Cytotoxic evaluation indicated that both types of vesicles and free sucrose distearate were safe for Caco-2 viability; however, free sucrose monostearate was toxic for the cells. As a conclusion of these preliminary studies, it can be stated that vesicles formed with mixtures of sucrose esters showed a size in the range of 200 nm maintaining their size when exposed to the action of a surfactant, but showing aggregation at acidic pH. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-02 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/32819 Chávez, Jorge; Romero, Eder Lilia; Morilla, María José; Valdés, Karina; Physicochemical characterization and cytotoxic studies of nonionic surfactant vesicles using sucrose esters as oral delivery systems; Elsevier; Colloids and Surfaces B: Biointerfaces; 117; 2-2014; 1-6 0927-7765 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/32819 |
identifier_str_mv |
Chávez, Jorge; Romero, Eder Lilia; Morilla, María José; Valdés, Karina; Physicochemical characterization and cytotoxic studies of nonionic surfactant vesicles using sucrose esters as oral delivery systems; Elsevier; Colloids and Surfaces B: Biointerfaces; 117; 2-2014; 1-6 0927-7765 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0927776514000307 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.colsurfb.2014.01.029 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613461872476160 |
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13.070432 |