Physicochemical characterization and cytotoxic studies of nonionic surfactant vesicles using sucrose esters as oral delivery systems

Autores
Valdés, Karina; Morilla, María José; Romero, Eder Lilia; Chávez, Jorge
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Several nanotechnological solutions for mucosal immunization have been proposed, such as nanoparticles, liposomes, solid lipidic particles, micelles, and surfactant vesicles. In recent years, surfactant vesicles have gained increasing scientific attention as an alternative potential drug delivery system to the conventional liposome. This type of vesicle known as niosomes or nonionic surfactant vesicles (NSVs) has a structure and properties similar to those of liposomes. Both of them can transport hydrophilic drugs by encapsulation in the aqueous inner pool or hydrophobic drugs by intercalation into hydrophobic domains. The aim of this study was to prepare and characterize vesicles formed by sucrose esters as protective systems of bioactive molecules for oral administration. Vesicles were prepared using two commercial products formed by mixtures of mono and diesters S-570 and S-770, respectively. Determined parameters were size and zeta potential; the stability of formulations was tested in presence of increasing concentrations of a surfactant, and at several pH values observed in the gastrointestinal tract. Solubilization experiences showed an initial decrease in size for vesicles of both ester mixtures, samples showed detergent resistance at higher Triton X-100 concentrations. Vesicles showed stability at pH 5–7.4 up to 90 min; however, both formulations showed colloidal instability at pH = 2, which corresponds to the isoelectric point of these vesicles. To evaluate the cytotoxicity of both vesicle formulations and separately each pure ester, Caco-2 cells were used. Cytotoxic evaluation indicated that both types of vesicles and free sucrose distearate were safe for Caco-2 viability; however, free sucrose monostearate was toxic for the cells. As a conclusion of these preliminary studies, it can be stated that vesicles formed with mixtures of sucrose esters showed a size in the range of 200 nm maintaining their size when exposed to the action of a surfactant, but showing aggregation at acidic pH.
Fil: Valdés, Karina. Universidad de Chile; Chile
Fil: Morilla, María José. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Romero, Eder Lilia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chávez, Jorge. Universidad de Chile; Chile
Materia
Nonionic Surfactant Vesicles
Sucrose Stearate
Cytotoxicity
Stability
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/32819
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/32819
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Physicochemical characterization and cytotoxic studies of nonionic surfactant vesicles using sucrose esters as oral delivery systemsValdés, KarinaMorilla, María JoséRomero, Eder LiliaChávez, JorgeNonionic Surfactant VesiclesSucrose StearateCytotoxicityStabilityhttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Several nanotechnological solutions for mucosal immunization have been proposed, such as nanoparticles, liposomes, solid lipidic particles, micelles, and surfactant vesicles. In recent years, surfactant vesicles have gained increasing scientific attention as an alternative potential drug delivery system to the conventional liposome. This type of vesicle known as niosomes or nonionic surfactant vesicles (NSVs) has a structure and properties similar to those of liposomes. Both of them can transport hydrophilic drugs by encapsulation in the aqueous inner pool or hydrophobic drugs by intercalation into hydrophobic domains. The aim of this study was to prepare and characterize vesicles formed by sucrose esters as protective systems of bioactive molecules for oral administration. Vesicles were prepared using two commercial products formed by mixtures of mono and diesters S-570 and S-770, respectively. Determined parameters were size and zeta potential; the stability of formulations was tested in presence of increasing concentrations of a surfactant, and at several pH values observed in the gastrointestinal tract. Solubilization experiences showed an initial decrease in size for vesicles of both ester mixtures, samples showed detergent resistance at higher Triton X-100 concentrations. Vesicles showed stability at pH 5–7.4 up to 90 min; however, both formulations showed colloidal instability at pH = 2, which corresponds to the isoelectric point of these vesicles. To evaluate the cytotoxicity of both vesicle formulations and separately each pure ester, Caco-2 cells were used. Cytotoxic evaluation indicated that both types of vesicles and free sucrose distearate were safe for Caco-2 viability; however, free sucrose monostearate was toxic for the cells. As a conclusion of these preliminary studies, it can be stated that vesicles formed with mixtures of sucrose esters showed a size in the range of 200 nm maintaining their size when exposed to the action of a surfactant, but showing aggregation at acidic pH.Fil: Valdés, Karina. Universidad de Chile; ChileFil: Morilla, María José. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Romero, Eder Lilia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Chávez, Jorge. Universidad de Chile; ChileElsevier2014-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/32819Chávez, Jorge; Romero, Eder Lilia; Morilla, María José; Valdés, Karina; Physicochemical characterization and cytotoxic studies of nonionic surfactant vesicles using sucrose esters as oral delivery systems; Elsevier; Colloids and Surfaces B: Biointerfaces; 117; 2-2014; 1-60927-7765CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0927776514000307info:eu-repo/semantics/altIdentifier/doi/10.1016/j.colsurfb.2014.01.029info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:40:31Zoai:ri.conicet.gov.ar:11336/32819instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:40:31.512CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Physicochemical characterization and cytotoxic studies of nonionic surfactant vesicles using sucrose esters as oral delivery systems
title Physicochemical characterization and cytotoxic studies of nonionic surfactant vesicles using sucrose esters as oral delivery systems
spellingShingle Physicochemical characterization and cytotoxic studies of nonionic surfactant vesicles using sucrose esters as oral delivery systems
Valdés, Karina
Nonionic Surfactant Vesicles
Sucrose Stearate
Cytotoxicity
Stability
title_short Physicochemical characterization and cytotoxic studies of nonionic surfactant vesicles using sucrose esters as oral delivery systems
title_full Physicochemical characterization and cytotoxic studies of nonionic surfactant vesicles using sucrose esters as oral delivery systems
title_fullStr Physicochemical characterization and cytotoxic studies of nonionic surfactant vesicles using sucrose esters as oral delivery systems
title_full_unstemmed Physicochemical characterization and cytotoxic studies of nonionic surfactant vesicles using sucrose esters as oral delivery systems
title_sort Physicochemical characterization and cytotoxic studies of nonionic surfactant vesicles using sucrose esters as oral delivery systems
dc.creator.none.fl_str_mv Valdés, Karina
Morilla, María José
Romero, Eder Lilia
Chávez, Jorge
author Valdés, Karina
author_facet Valdés, Karina
Morilla, María José
Romero, Eder Lilia
Chávez, Jorge
author_role author
author2 Morilla, María José
Romero, Eder Lilia
Chávez, Jorge
author2_role author
author
author
dc.subject.none.fl_str_mv Nonionic Surfactant Vesicles
Sucrose Stearate
Cytotoxicity
Stability
topic Nonionic Surfactant Vesicles
Sucrose Stearate
Cytotoxicity
Stability
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Several nanotechnological solutions for mucosal immunization have been proposed, such as nanoparticles, liposomes, solid lipidic particles, micelles, and surfactant vesicles. In recent years, surfactant vesicles have gained increasing scientific attention as an alternative potential drug delivery system to the conventional liposome. This type of vesicle known as niosomes or nonionic surfactant vesicles (NSVs) has a structure and properties similar to those of liposomes. Both of them can transport hydrophilic drugs by encapsulation in the aqueous inner pool or hydrophobic drugs by intercalation into hydrophobic domains. The aim of this study was to prepare and characterize vesicles formed by sucrose esters as protective systems of bioactive molecules for oral administration. Vesicles were prepared using two commercial products formed by mixtures of mono and diesters S-570 and S-770, respectively. Determined parameters were size and zeta potential; the stability of formulations was tested in presence of increasing concentrations of a surfactant, and at several pH values observed in the gastrointestinal tract. Solubilization experiences showed an initial decrease in size for vesicles of both ester mixtures, samples showed detergent resistance at higher Triton X-100 concentrations. Vesicles showed stability at pH 5–7.4 up to 90 min; however, both formulations showed colloidal instability at pH = 2, which corresponds to the isoelectric point of these vesicles. To evaluate the cytotoxicity of both vesicle formulations and separately each pure ester, Caco-2 cells were used. Cytotoxic evaluation indicated that both types of vesicles and free sucrose distearate were safe for Caco-2 viability; however, free sucrose monostearate was toxic for the cells. As a conclusion of these preliminary studies, it can be stated that vesicles formed with mixtures of sucrose esters showed a size in the range of 200 nm maintaining their size when exposed to the action of a surfactant, but showing aggregation at acidic pH.
Fil: Valdés, Karina. Universidad de Chile; Chile
Fil: Morilla, María José. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Romero, Eder Lilia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chávez, Jorge. Universidad de Chile; Chile
description Several nanotechnological solutions for mucosal immunization have been proposed, such as nanoparticles, liposomes, solid lipidic particles, micelles, and surfactant vesicles. In recent years, surfactant vesicles have gained increasing scientific attention as an alternative potential drug delivery system to the conventional liposome. This type of vesicle known as niosomes or nonionic surfactant vesicles (NSVs) has a structure and properties similar to those of liposomes. Both of them can transport hydrophilic drugs by encapsulation in the aqueous inner pool or hydrophobic drugs by intercalation into hydrophobic domains. The aim of this study was to prepare and characterize vesicles formed by sucrose esters as protective systems of bioactive molecules for oral administration. Vesicles were prepared using two commercial products formed by mixtures of mono and diesters S-570 and S-770, respectively. Determined parameters were size and zeta potential; the stability of formulations was tested in presence of increasing concentrations of a surfactant, and at several pH values observed in the gastrointestinal tract. Solubilization experiences showed an initial decrease in size for vesicles of both ester mixtures, samples showed detergent resistance at higher Triton X-100 concentrations. Vesicles showed stability at pH 5–7.4 up to 90 min; however, both formulations showed colloidal instability at pH = 2, which corresponds to the isoelectric point of these vesicles. To evaluate the cytotoxicity of both vesicle formulations and separately each pure ester, Caco-2 cells were used. Cytotoxic evaluation indicated that both types of vesicles and free sucrose distearate were safe for Caco-2 viability; however, free sucrose monostearate was toxic for the cells. As a conclusion of these preliminary studies, it can be stated that vesicles formed with mixtures of sucrose esters showed a size in the range of 200 nm maintaining their size when exposed to the action of a surfactant, but showing aggregation at acidic pH.
publishDate 2014
dc.date.none.fl_str_mv 2014-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/32819
Chávez, Jorge; Romero, Eder Lilia; Morilla, María José; Valdés, Karina; Physicochemical characterization and cytotoxic studies of nonionic surfactant vesicles using sucrose esters as oral delivery systems; Elsevier; Colloids and Surfaces B: Biointerfaces; 117; 2-2014; 1-6
0927-7765
CONICET Digital
CONICET
url http://hdl.handle.net/11336/32819
identifier_str_mv Chávez, Jorge; Romero, Eder Lilia; Morilla, María José; Valdés, Karina; Physicochemical characterization and cytotoxic studies of nonionic surfactant vesicles using sucrose esters as oral delivery systems; Elsevier; Colloids and Surfaces B: Biointerfaces; 117; 2-2014; 1-6
0927-7765
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0927776514000307
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.colsurfb.2014.01.029
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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