Assessment of the Effect of Triton X-114 on the Physicochemical Properties of an Antibody Fragment

Autores
Pellegrini Malpiedi, Luciana; Nerli, Bibiana Beatriz; Abdalla, Dulcinéia Saes Parra; Pessoa, Adalberto Jr.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The effect of Triton X-114 on the physicochemical properties of a single-chain antibody fragment (scFv) has been studied. According to the far UV circular dichroism spectroscopy, the secondary structure of the recombinant antibody was not significantly affected by the presence of Triton. From the antibody tertiary structure analysis, it was found that the surfactant could be located around the tryptophan molecules accessible to the solvent, diminishing the polarity of its environment but maintaining most of the protein structure integrity. However, in certain conditions of high temperature and high concentration of denaturant molecules, the presence of TX could compromise the antibody fragment stability. These results represent a previous step in designing scFv purification protocols and should be considered prior to developing scFv liquid–liquid extraction procedures
Fil: Pellegrini Malpiedi, Luciana. Universidade de Sao Paulo; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Quimicos Rosario. Universidad Nacional de Rosario. Facultad de Cs.bioquímicas y Farmaceuticas. Instituto de Procesos Biotecnológicos y Quimicos Rosario; Argentina
Fil: Nerli, Bibiana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Quimicos Rosario. Universidad Nacional de Rosario. Facultad de Cs.bioquímicas y Farmaceuticas. Instituto de Procesos Biotecnológicos y Quimicos Rosario; Argentina
Fil: Abdalla, Dulcinéia Saes Parra. Universidade de Sao Paulo; Brasil
Fil: Pessoa, Adalberto Jr.. Universidade de Sao Paulo; Brasil
Materia
Nonionic Surfactant
Single-Chain Antibody Fragment
Spectroscopic Study
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/30302
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/30302
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Assessment of the Effect of Triton X-114 on the Physicochemical Properties of an Antibody FragmentPellegrini Malpiedi, LucianaNerli, Bibiana BeatrizAbdalla, Dulcinéia Saes ParraPessoa, Adalberto Jr.Nonionic SurfactantSingle-Chain Antibody FragmentSpectroscopic Studyhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The effect of Triton X-114 on the physicochemical properties of a single-chain antibody fragment (scFv) has been studied. According to the far UV circular dichroism spectroscopy, the secondary structure of the recombinant antibody was not significantly affected by the presence of Triton. From the antibody tertiary structure analysis, it was found that the surfactant could be located around the tryptophan molecules accessible to the solvent, diminishing the polarity of its environment but maintaining most of the protein structure integrity. However, in certain conditions of high temperature and high concentration of denaturant molecules, the presence of TX could compromise the antibody fragment stability. These results represent a previous step in designing scFv purification protocols and should be considered prior to developing scFv liquid–liquid extraction proceduresFil: Pellegrini Malpiedi, Luciana. Universidade de Sao Paulo; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Quimicos Rosario. Universidad Nacional de Rosario. Facultad de Cs.bioquímicas y Farmaceuticas. Instituto de Procesos Biotecnológicos y Quimicos Rosario; ArgentinaFil: Nerli, Bibiana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Quimicos Rosario. Universidad Nacional de Rosario. Facultad de Cs.bioquímicas y Farmaceuticas. Instituto de Procesos Biotecnológicos y Quimicos Rosario; ArgentinaFil: Abdalla, Dulcinéia Saes Parra. Universidade de Sao Paulo; BrasilFil: Pessoa, Adalberto Jr.. Universidade de Sao Paulo; BrasilAmerican Chemical Society2014-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/30302Pellegrini Malpiedi, Luciana; Nerli, Bibiana Beatriz; Abdalla, Dulcinéia Saes Parra; Pessoa, Adalberto Jr.; Assessment of the Effect of Triton X-114 on the Physicochemical Properties of an Antibody Fragment; American Chemical Society; Biotechnology Progress; 30; 3; 5-2014; 554-5618756-7938CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/btpr.1882info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/btpr.1882/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:38:05Zoai:ri.conicet.gov.ar:11336/30302instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:38:05.607CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Assessment of the Effect of Triton X-114 on the Physicochemical Properties of an Antibody Fragment
title Assessment of the Effect of Triton X-114 on the Physicochemical Properties of an Antibody Fragment
spellingShingle Assessment of the Effect of Triton X-114 on the Physicochemical Properties of an Antibody Fragment
Pellegrini Malpiedi, Luciana
Nonionic Surfactant
Single-Chain Antibody Fragment
Spectroscopic Study
title_short Assessment of the Effect of Triton X-114 on the Physicochemical Properties of an Antibody Fragment
title_full Assessment of the Effect of Triton X-114 on the Physicochemical Properties of an Antibody Fragment
title_fullStr Assessment of the Effect of Triton X-114 on the Physicochemical Properties of an Antibody Fragment
title_full_unstemmed Assessment of the Effect of Triton X-114 on the Physicochemical Properties of an Antibody Fragment
title_sort Assessment of the Effect of Triton X-114 on the Physicochemical Properties of an Antibody Fragment
dc.creator.none.fl_str_mv Pellegrini Malpiedi, Luciana
Nerli, Bibiana Beatriz
Abdalla, Dulcinéia Saes Parra
Pessoa, Adalberto Jr.
author Pellegrini Malpiedi, Luciana
author_facet Pellegrini Malpiedi, Luciana
Nerli, Bibiana Beatriz
Abdalla, Dulcinéia Saes Parra
Pessoa, Adalberto Jr.
author_role author
author2 Nerli, Bibiana Beatriz
Abdalla, Dulcinéia Saes Parra
Pessoa, Adalberto Jr.
author2_role author
author
author
dc.subject.none.fl_str_mv Nonionic Surfactant
Single-Chain Antibody Fragment
Spectroscopic Study
topic Nonionic Surfactant
Single-Chain Antibody Fragment
Spectroscopic Study
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The effect of Triton X-114 on the physicochemical properties of a single-chain antibody fragment (scFv) has been studied. According to the far UV circular dichroism spectroscopy, the secondary structure of the recombinant antibody was not significantly affected by the presence of Triton. From the antibody tertiary structure analysis, it was found that the surfactant could be located around the tryptophan molecules accessible to the solvent, diminishing the polarity of its environment but maintaining most of the protein structure integrity. However, in certain conditions of high temperature and high concentration of denaturant molecules, the presence of TX could compromise the antibody fragment stability. These results represent a previous step in designing scFv purification protocols and should be considered prior to developing scFv liquid–liquid extraction procedures
Fil: Pellegrini Malpiedi, Luciana. Universidade de Sao Paulo; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Quimicos Rosario. Universidad Nacional de Rosario. Facultad de Cs.bioquímicas y Farmaceuticas. Instituto de Procesos Biotecnológicos y Quimicos Rosario; Argentina
Fil: Nerli, Bibiana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Quimicos Rosario. Universidad Nacional de Rosario. Facultad de Cs.bioquímicas y Farmaceuticas. Instituto de Procesos Biotecnológicos y Quimicos Rosario; Argentina
Fil: Abdalla, Dulcinéia Saes Parra. Universidade de Sao Paulo; Brasil
Fil: Pessoa, Adalberto Jr.. Universidade de Sao Paulo; Brasil
description The effect of Triton X-114 on the physicochemical properties of a single-chain antibody fragment (scFv) has been studied. According to the far UV circular dichroism spectroscopy, the secondary structure of the recombinant antibody was not significantly affected by the presence of Triton. From the antibody tertiary structure analysis, it was found that the surfactant could be located around the tryptophan molecules accessible to the solvent, diminishing the polarity of its environment but maintaining most of the protein structure integrity. However, in certain conditions of high temperature and high concentration of denaturant molecules, the presence of TX could compromise the antibody fragment stability. These results represent a previous step in designing scFv purification protocols and should be considered prior to developing scFv liquid–liquid extraction procedures
publishDate 2014
dc.date.none.fl_str_mv 2014-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/30302
Pellegrini Malpiedi, Luciana; Nerli, Bibiana Beatriz; Abdalla, Dulcinéia Saes Parra; Pessoa, Adalberto Jr.; Assessment of the Effect of Triton X-114 on the Physicochemical Properties of an Antibody Fragment; American Chemical Society; Biotechnology Progress; 30; 3; 5-2014; 554-561
8756-7938
CONICET Digital
CONICET
url http://hdl.handle.net/11336/30302
identifier_str_mv Pellegrini Malpiedi, Luciana; Nerli, Bibiana Beatriz; Abdalla, Dulcinéia Saes Parra; Pessoa, Adalberto Jr.; Assessment of the Effect of Triton X-114 on the Physicochemical Properties of an Antibody Fragment; American Chemical Society; Biotechnology Progress; 30; 3; 5-2014; 554-561
8756-7938
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/btpr.1882
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/btpr.1882/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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