Altered lipidome and antioxidative activity of small, dense HDL in normolipidemic rheumatoid arthritis: Relevance of inflammation

Autores
Gomez Rosso, Leonardo Adrián; Lhomme, Marie; Meroño, Tomás; Sorroche, Patricia Beatriz; Catoggio, Luis; Soriano, Enrique; Saucedo, Carla; Malah, Verónica; Dauteuille, Carolane; Boero, Laura Estela; Lesnik, Philippe; Robillard, Paul; Chapman, M. John; Brites, Fernando Daniel; Kontush, Anatol
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
OBJECTIVE: High-density lipoprotein (HDL) particles exert potent antiatherogenic activities, including antioxidative actions, which are relevant to attenuation of atherosclerosis progression. Such activities are enriched in small, dense HDL and can be compromised under conditions of chronic inflammation like rheumatoid arthritis (RA). However, structure-function relationships of HDL largely remain indeterminate. METHODS: The relationships between HDL structure and function were evaluated in normolipidemic patients with active RA (DAS28 > 3.2; n = 12) and in normolipidemic age-matched controls (n = 10). Small, dense HDL3b and 3c particles were isolated from plasma or serum by density gradient ultracentrifugation and their physicochemical characteristics, lipidome (by LC/MS/MS) and antioxidative function (as protection of normolipidemic LDL from free radical-induced oxidation) were evaluated. RESULTS: As expected, active RA patients featured significantly elevated plasma levels of high-sensitivity C-reactive protein (hsCRP; p < 0.001) and serum amyloid A (SAA; p < 0.01) relative to controls. Antioxidative activity and weight % chemical composition of small, dense HDL did not differ between RA patients and controls (p > 0.05), whereas HDL phosphosphingolipidome was significantly altered in RA. Subgroup analyses revealed that RA patients featuring high levels of inflammation (hsCRP>10 mg/l) possessed small, dense HDL with reduced antioxidative activities (p < 0.01). Furthermore, antioxidative activity of HDL was inversely correlated with plasma hsCRP (p < 0.01). CONCLUSIONS: These data revealed that (i) despite normolipidemic state, the lipidome of small, dense HDL was altered in RA and (ii) high levels of inflammation can be responsible for the functional deficiency of small, dense HDL in RA.
Fil: Gomez Rosso, Leonardo Adrián. Inserm; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Lhomme, Marie. Inserm; Francia. Universite Pierre et Marie Curie; Francia
Fil: Meroño, Tomás. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Sorroche, Patricia Beatriz. Instituto Universidad Escuela de Medicina del Hospital Italiano; Argentina
Fil: Catoggio, Luis. Instituto Universidad Escuela de Medicina del Hospital Italiano; Argentina
Fil: Soriano, Enrique. Instituto Universidad Escuela de Medicina del Hospital Italiano; Argentina
Fil: Saucedo, Carla. Instituto Universidad Escuela de Medicina del Hospital Italiano; Argentina
Fil: Malah, Verónica. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Dauteuille, Carolane. Inserm; Francia. Universite Pierre et Marie Curie; Francia
Fil: Boero, Laura Estela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Lesnik, Philippe. Inserm; Francia. Universite Pierre et Marie Curie; Francia
Fil: Robillard, Paul. Inserm; Francia. Universite Pierre et Marie Curie; Francia
Fil: Chapman, M. John. Inserm; Francia. Universite Pierre et Marie Curie; Francia
Fil: Brites, Fernando Daniel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Kontush, Anatol. Inserm; Francia. Universite Pierre et Marie Curie; Francia
Materia
Antioxidative Activity
Cardiovascular Disease
Inflammation
Rheumatoid Arthritis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/30536
Acceder
id CONICETDig_c3a8fe9d87d2ffdaef360c366eb490c9
oai_identifier_str oai:ri.conicet.gov.ar:11336/30536
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Altered lipidome and antioxidative activity of small, dense HDL in normolipidemic rheumatoid arthritis: Relevance of inflammationGomez Rosso, Leonardo AdriánLhomme, MarieMeroño, TomásSorroche, Patricia BeatrizCatoggio, LuisSoriano, EnriqueSaucedo, CarlaMalah, VerónicaDauteuille, CarolaneBoero, Laura EstelaLesnik, PhilippeRobillard, PaulChapman, M. JohnBrites, Fernando DanielKontush, AnatolAntioxidative ActivityCardiovascular DiseaseInflammationRheumatoid Arthritishttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3OBJECTIVE: High-density lipoprotein (HDL) particles exert potent antiatherogenic activities, including antioxidative actions, which are relevant to attenuation of atherosclerosis progression. Such activities are enriched in small, dense HDL and can be compromised under conditions of chronic inflammation like rheumatoid arthritis (RA). However, structure-function relationships of HDL largely remain indeterminate. METHODS: The relationships between HDL structure and function were evaluated in normolipidemic patients with active RA (DAS28 > 3.2; n = 12) and in normolipidemic age-matched controls (n = 10). Small, dense HDL3b and 3c particles were isolated from plasma or serum by density gradient ultracentrifugation and their physicochemical characteristics, lipidome (by LC/MS/MS) and antioxidative function (as protection of normolipidemic LDL from free radical-induced oxidation) were evaluated. RESULTS: As expected, active RA patients featured significantly elevated plasma levels of high-sensitivity C-reactive protein (hsCRP; p < 0.001) and serum amyloid A (SAA; p < 0.01) relative to controls. Antioxidative activity and weight % chemical composition of small, dense HDL did not differ between RA patients and controls (p > 0.05), whereas HDL phosphosphingolipidome was significantly altered in RA. Subgroup analyses revealed that RA patients featuring high levels of inflammation (hsCRP>10 mg/l) possessed small, dense HDL with reduced antioxidative activities (p < 0.01). Furthermore, antioxidative activity of HDL was inversely correlated with plasma hsCRP (p < 0.01). CONCLUSIONS: These data revealed that (i) despite normolipidemic state, the lipidome of small, dense HDL was altered in RA and (ii) high levels of inflammation can be responsible for the functional deficiency of small, dense HDL in RA.Fil: Gomez Rosso, Leonardo Adrián. Inserm; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Lhomme, Marie. Inserm; Francia. Universite Pierre et Marie Curie; FranciaFil: Meroño, Tomás. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Sorroche, Patricia Beatriz. Instituto Universidad Escuela de Medicina del Hospital Italiano; ArgentinaFil: Catoggio, Luis. Instituto Universidad Escuela de Medicina del Hospital Italiano; ArgentinaFil: Soriano, Enrique. Instituto Universidad Escuela de Medicina del Hospital Italiano; ArgentinaFil: Saucedo, Carla. Instituto Universidad Escuela de Medicina del Hospital Italiano; ArgentinaFil: Malah, Verónica. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaFil: Dauteuille, Carolane. Inserm; Francia. Universite Pierre et Marie Curie; FranciaFil: Boero, Laura Estela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Lesnik, Philippe. Inserm; Francia. Universite Pierre et Marie Curie; FranciaFil: Robillard, Paul. Inserm; Francia. Universite Pierre et Marie Curie; FranciaFil: Chapman, M. John. Inserm; Francia. Universite Pierre et Marie Curie; FranciaFil: Brites, Fernando Daniel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Kontush, Anatol. Inserm; Francia. Universite Pierre et Marie Curie; FranciaElsevier Ireland2014-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/30536Gomez Rosso, Leonardo Adrián; Lhomme, Marie; Meroño, Tomás; Sorroche, Patricia Beatriz; Catoggio, Luis; et al.; Altered lipidome and antioxidative activity of small, dense HDL in normolipidemic rheumatoid arthritis: Relevance of inflammation; Elsevier Ireland; Atherosclerosis; 237; 2; 10-2014; 652-6600021-9150CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.atherosclerosis.2014.09.034info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0021915014014579info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:02:37Zoai:ri.conicet.gov.ar:11336/30536instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:02:37.579CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Altered lipidome and antioxidative activity of small, dense HDL in normolipidemic rheumatoid arthritis: Relevance of inflammation
title Altered lipidome and antioxidative activity of small, dense HDL in normolipidemic rheumatoid arthritis: Relevance of inflammation
spellingShingle Altered lipidome and antioxidative activity of small, dense HDL in normolipidemic rheumatoid arthritis: Relevance of inflammation
Gomez Rosso, Leonardo Adrián
Antioxidative Activity
Cardiovascular Disease
Inflammation
Rheumatoid Arthritis
title_short Altered lipidome and antioxidative activity of small, dense HDL in normolipidemic rheumatoid arthritis: Relevance of inflammation
title_full Altered lipidome and antioxidative activity of small, dense HDL in normolipidemic rheumatoid arthritis: Relevance of inflammation
title_fullStr Altered lipidome and antioxidative activity of small, dense HDL in normolipidemic rheumatoid arthritis: Relevance of inflammation
title_full_unstemmed Altered lipidome and antioxidative activity of small, dense HDL in normolipidemic rheumatoid arthritis: Relevance of inflammation
title_sort Altered lipidome and antioxidative activity of small, dense HDL in normolipidemic rheumatoid arthritis: Relevance of inflammation
dc.creator.none.fl_str_mv Gomez Rosso, Leonardo Adrián
Lhomme, Marie
Meroño, Tomás
Sorroche, Patricia Beatriz
Catoggio, Luis
Soriano, Enrique
Saucedo, Carla
Malah, Verónica
Dauteuille, Carolane
Boero, Laura Estela
Lesnik, Philippe
Robillard, Paul
Chapman, M. John
Brites, Fernando Daniel
Kontush, Anatol
author Gomez Rosso, Leonardo Adrián
author_facet Gomez Rosso, Leonardo Adrián
Lhomme, Marie
Meroño, Tomás
Sorroche, Patricia Beatriz
Catoggio, Luis
Soriano, Enrique
Saucedo, Carla
Malah, Verónica
Dauteuille, Carolane
Boero, Laura Estela
Lesnik, Philippe
Robillard, Paul
Chapman, M. John
Brites, Fernando Daniel
Kontush, Anatol
author_role author
author2 Lhomme, Marie
Meroño, Tomás
Sorroche, Patricia Beatriz
Catoggio, Luis
Soriano, Enrique
Saucedo, Carla
Malah, Verónica
Dauteuille, Carolane
Boero, Laura Estela
Lesnik, Philippe
Robillard, Paul
Chapman, M. John
Brites, Fernando Daniel
Kontush, Anatol
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Antioxidative Activity
Cardiovascular Disease
Inflammation
Rheumatoid Arthritis
topic Antioxidative Activity
Cardiovascular Disease
Inflammation
Rheumatoid Arthritis
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv OBJECTIVE: High-density lipoprotein (HDL) particles exert potent antiatherogenic activities, including antioxidative actions, which are relevant to attenuation of atherosclerosis progression. Such activities are enriched in small, dense HDL and can be compromised under conditions of chronic inflammation like rheumatoid arthritis (RA). However, structure-function relationships of HDL largely remain indeterminate. METHODS: The relationships between HDL structure and function were evaluated in normolipidemic patients with active RA (DAS28 > 3.2; n = 12) and in normolipidemic age-matched controls (n = 10). Small, dense HDL3b and 3c particles were isolated from plasma or serum by density gradient ultracentrifugation and their physicochemical characteristics, lipidome (by LC/MS/MS) and antioxidative function (as protection of normolipidemic LDL from free radical-induced oxidation) were evaluated. RESULTS: As expected, active RA patients featured significantly elevated plasma levels of high-sensitivity C-reactive protein (hsCRP; p < 0.001) and serum amyloid A (SAA; p < 0.01) relative to controls. Antioxidative activity and weight % chemical composition of small, dense HDL did not differ between RA patients and controls (p > 0.05), whereas HDL phosphosphingolipidome was significantly altered in RA. Subgroup analyses revealed that RA patients featuring high levels of inflammation (hsCRP>10 mg/l) possessed small, dense HDL with reduced antioxidative activities (p < 0.01). Furthermore, antioxidative activity of HDL was inversely correlated with plasma hsCRP (p < 0.01). CONCLUSIONS: These data revealed that (i) despite normolipidemic state, the lipidome of small, dense HDL was altered in RA and (ii) high levels of inflammation can be responsible for the functional deficiency of small, dense HDL in RA.
Fil: Gomez Rosso, Leonardo Adrián. Inserm; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Lhomme, Marie. Inserm; Francia. Universite Pierre et Marie Curie; Francia
Fil: Meroño, Tomás. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Sorroche, Patricia Beatriz. Instituto Universidad Escuela de Medicina del Hospital Italiano; Argentina
Fil: Catoggio, Luis. Instituto Universidad Escuela de Medicina del Hospital Italiano; Argentina
Fil: Soriano, Enrique. Instituto Universidad Escuela de Medicina del Hospital Italiano; Argentina
Fil: Saucedo, Carla. Instituto Universidad Escuela de Medicina del Hospital Italiano; Argentina
Fil: Malah, Verónica. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Dauteuille, Carolane. Inserm; Francia. Universite Pierre et Marie Curie; Francia
Fil: Boero, Laura Estela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Lesnik, Philippe. Inserm; Francia. Universite Pierre et Marie Curie; Francia
Fil: Robillard, Paul. Inserm; Francia. Universite Pierre et Marie Curie; Francia
Fil: Chapman, M. John. Inserm; Francia. Universite Pierre et Marie Curie; Francia
Fil: Brites, Fernando Daniel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Kontush, Anatol. Inserm; Francia. Universite Pierre et Marie Curie; Francia
description OBJECTIVE: High-density lipoprotein (HDL) particles exert potent antiatherogenic activities, including antioxidative actions, which are relevant to attenuation of atherosclerosis progression. Such activities are enriched in small, dense HDL and can be compromised under conditions of chronic inflammation like rheumatoid arthritis (RA). However, structure-function relationships of HDL largely remain indeterminate. METHODS: The relationships between HDL structure and function were evaluated in normolipidemic patients with active RA (DAS28 > 3.2; n = 12) and in normolipidemic age-matched controls (n = 10). Small, dense HDL3b and 3c particles were isolated from plasma or serum by density gradient ultracentrifugation and their physicochemical characteristics, lipidome (by LC/MS/MS) and antioxidative function (as protection of normolipidemic LDL from free radical-induced oxidation) were evaluated. RESULTS: As expected, active RA patients featured significantly elevated plasma levels of high-sensitivity C-reactive protein (hsCRP; p < 0.001) and serum amyloid A (SAA; p < 0.01) relative to controls. Antioxidative activity and weight % chemical composition of small, dense HDL did not differ between RA patients and controls (p > 0.05), whereas HDL phosphosphingolipidome was significantly altered in RA. Subgroup analyses revealed that RA patients featuring high levels of inflammation (hsCRP>10 mg/l) possessed small, dense HDL with reduced antioxidative activities (p < 0.01). Furthermore, antioxidative activity of HDL was inversely correlated with plasma hsCRP (p < 0.01). CONCLUSIONS: These data revealed that (i) despite normolipidemic state, the lipidome of small, dense HDL was altered in RA and (ii) high levels of inflammation can be responsible for the functional deficiency of small, dense HDL in RA.
publishDate 2014
dc.date.none.fl_str_mv 2014-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/30536
Gomez Rosso, Leonardo Adrián; Lhomme, Marie; Meroño, Tomás; Sorroche, Patricia Beatriz; Catoggio, Luis; et al.; Altered lipidome and antioxidative activity of small, dense HDL in normolipidemic rheumatoid arthritis: Relevance of inflammation; Elsevier Ireland; Atherosclerosis; 237; 2; 10-2014; 652-660
0021-9150
CONICET Digital
CONICET
url http://hdl.handle.net/11336/30536
identifier_str_mv Gomez Rosso, Leonardo Adrián; Lhomme, Marie; Meroño, Tomás; Sorroche, Patricia Beatriz; Catoggio, Luis; et al.; Altered lipidome and antioxidative activity of small, dense HDL in normolipidemic rheumatoid arthritis: Relevance of inflammation; Elsevier Ireland; Atherosclerosis; 237; 2; 10-2014; 652-660
0021-9150
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.atherosclerosis.2014.09.034
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0021915014014579
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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