Differences in phosphatidic acid signalling and metabolism between ABA and GA treatments of barley aleurone cells

Autores
Villasuso, Ana Laura; Di Palma, María Albana; Aveldaño, Marta Isabel; Pasquaré, Susana J.; Racagni, Graciela; Giusto, Norma Maria; Machado, Estela E.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Phosphatidic acid (PA) is the common lipid product in abscisic acid (ABA) and gibberellic acid (GA)response. In this work we investigated the lipid metabolism in response to both hormones. We could detect an in vivo phospholipase D activity (PLD, EC 3.1.4.4). This PLD produced [32P]PA (phosphatidic acid) rapidly (minutes) in the presence of ABA, confirming PA involvement in signal transduction, and transiently, indicating rapid PA removal after generation. The presence of PA removal by phosphatidate phosphatase 1 and 2 isoforms (E.C. 3.1.3.4) was verified in isolated aleurone membranes in vitro, the former but not the latter being Specifically responsive to the presence of GA or ABA. The in vitro DGPP phosphatase activity was not modified by short time incubation with GA or ABA while the in vitro PA kinase e that allows the production of 18:2-DGPP from 18:2-PA e is stimulated by ABA. The long term effects (24 h) of ABA or GA on lipid and fatty acid composition of aleurone layer cells were then investigated. An increase in PC and, to a lesser extent, in PE levels is the consequence of both hormone treatments. ABA, in aleurone layer cells, specifically activates a PLD whose product, PA, could be the substrate of PAP1 and/or PAK activities. Neither PLD nor PAK activation can be monitored by GA treatment. The increase in PAP1 activity monitored after ABA or GA treatment might participate in the increase in PC level observed after 24 h hormone incubation
Fil: Villasuso, Ana Laura. Universidad Nacional de Rio Cuarto; Argentina
Fil: Di Palma, María Albana. Universidad Nacional de Rio Cuarto; Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina
Fil: Pasquaré, Susana J.. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina
Fil: Racagni, Graciela. Universidad Nacional de Rio Cuarto; Argentina
Fil: Giusto, Norma Maria. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina
Fil: Machado, Estela E.. Universidad Nacional de Rio Cuarto; Argentina
Materia
Aleurona
Abscisic Acid
Diacylglycerol Pyrophosphate
Gibberellic Acid
Phosphatidic Acid
Phosphatidate Phosphatases
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7393
Acceder
id CONICETDig_088391c5a318217831d13a43cbe97457
oai_identifier_str oai:ri.conicet.gov.ar:11336/7393
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Differences in phosphatidic acid signalling and metabolism between ABA and GA treatments of barley aleurone cellsVillasuso, Ana LauraDi Palma, María AlbanaAveldaño, Marta IsabelPasquaré, Susana J.Racagni, GracielaGiusto, Norma MariaMachado, Estela E.AleuronaAbscisic AcidDiacylglycerol PyrophosphateGibberellic AcidPhosphatidic AcidPhosphatidate Phosphataseshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Phosphatidic acid (PA) is the common lipid product in abscisic acid (ABA) and gibberellic acid (GA)response. In this work we investigated the lipid metabolism in response to both hormones. We could detect an in vivo phospholipase D activity (PLD, EC 3.1.4.4). This PLD produced [32P]PA (phosphatidic acid) rapidly (minutes) in the presence of ABA, confirming PA involvement in signal transduction, and transiently, indicating rapid PA removal after generation. The presence of PA removal by phosphatidate phosphatase 1 and 2 isoforms (E.C. 3.1.3.4) was verified in isolated aleurone membranes in vitro, the former but not the latter being Specifically responsive to the presence of GA or ABA. The in vitro DGPP phosphatase activity was not modified by short time incubation with GA or ABA while the in vitro PA kinase e that allows the production of 18:2-DGPP from 18:2-PA e is stimulated by ABA. The long term effects (24 h) of ABA or GA on lipid and fatty acid composition of aleurone layer cells were then investigated. An increase in PC and, to a lesser extent, in PE levels is the consequence of both hormone treatments. ABA, in aleurone layer cells, specifically activates a PLD whose product, PA, could be the substrate of PAP1 and/or PAK activities. Neither PLD nor PAK activation can be monitored by GA treatment. The increase in PAP1 activity monitored after ABA or GA treatment might participate in the increase in PC level observed after 24 h hormone incubationFil: Villasuso, Ana Laura. Universidad Nacional de Rio Cuarto; ArgentinaFil: Di Palma, María Albana. Universidad Nacional de Rio Cuarto; ArgentinaFil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); ArgentinaFil: Pasquaré, Susana J.. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); ArgentinaFil: Racagni, Graciela. Universidad Nacional de Rio Cuarto; ArgentinaFil: Giusto, Norma Maria. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); ArgentinaFil: Machado, Estela E.. Universidad Nacional de Rio Cuarto; ArgentinaElsevier Masson2013-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7393Villasuso, Ana Laura; Di Palma, María Albana; Aveldaño, Marta Isabel; Pasquaré, Susana J.; Racagni, Graciela; et al.; Differences in phosphatidic acid signalling and metabolism between ABA and GA treatments of barley aleurone cells; Elsevier Masson; Plant Physiology And Biochemistry; 65; 2-2013; 1-80981-9428enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0981942813000247info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plaphy.2013.01.005info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:51:32Zoai:ri.conicet.gov.ar:11336/7393instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:51:32.938CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Differences in phosphatidic acid signalling and metabolism between ABA and GA treatments of barley aleurone cells
title Differences in phosphatidic acid signalling and metabolism between ABA and GA treatments of barley aleurone cells
spellingShingle Differences in phosphatidic acid signalling and metabolism between ABA and GA treatments of barley aleurone cells
Villasuso, Ana Laura
Aleurona
Abscisic Acid
Diacylglycerol Pyrophosphate
Gibberellic Acid
Phosphatidic Acid
Phosphatidate Phosphatases
title_short Differences in phosphatidic acid signalling and metabolism between ABA and GA treatments of barley aleurone cells
title_full Differences in phosphatidic acid signalling and metabolism between ABA and GA treatments of barley aleurone cells
title_fullStr Differences in phosphatidic acid signalling and metabolism between ABA and GA treatments of barley aleurone cells
title_full_unstemmed Differences in phosphatidic acid signalling and metabolism between ABA and GA treatments of barley aleurone cells
title_sort Differences in phosphatidic acid signalling and metabolism between ABA and GA treatments of barley aleurone cells
dc.creator.none.fl_str_mv Villasuso, Ana Laura
Di Palma, María Albana
Aveldaño, Marta Isabel
Pasquaré, Susana J.
Racagni, Graciela
Giusto, Norma Maria
Machado, Estela E.
author Villasuso, Ana Laura
author_facet Villasuso, Ana Laura
Di Palma, María Albana
Aveldaño, Marta Isabel
Pasquaré, Susana J.
Racagni, Graciela
Giusto, Norma Maria
Machado, Estela E.
author_role author
author2 Di Palma, María Albana
Aveldaño, Marta Isabel
Pasquaré, Susana J.
Racagni, Graciela
Giusto, Norma Maria
Machado, Estela E.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Aleurona
Abscisic Acid
Diacylglycerol Pyrophosphate
Gibberellic Acid
Phosphatidic Acid
Phosphatidate Phosphatases
topic Aleurona
Abscisic Acid
Diacylglycerol Pyrophosphate
Gibberellic Acid
Phosphatidic Acid
Phosphatidate Phosphatases
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Phosphatidic acid (PA) is the common lipid product in abscisic acid (ABA) and gibberellic acid (GA)response. In this work we investigated the lipid metabolism in response to both hormones. We could detect an in vivo phospholipase D activity (PLD, EC 3.1.4.4). This PLD produced [32P]PA (phosphatidic acid) rapidly (minutes) in the presence of ABA, confirming PA involvement in signal transduction, and transiently, indicating rapid PA removal after generation. The presence of PA removal by phosphatidate phosphatase 1 and 2 isoforms (E.C. 3.1.3.4) was verified in isolated aleurone membranes in vitro, the former but not the latter being Specifically responsive to the presence of GA or ABA. The in vitro DGPP phosphatase activity was not modified by short time incubation with GA or ABA while the in vitro PA kinase e that allows the production of 18:2-DGPP from 18:2-PA e is stimulated by ABA. The long term effects (24 h) of ABA or GA on lipid and fatty acid composition of aleurone layer cells were then investigated. An increase in PC and, to a lesser extent, in PE levels is the consequence of both hormone treatments. ABA, in aleurone layer cells, specifically activates a PLD whose product, PA, could be the substrate of PAP1 and/or PAK activities. Neither PLD nor PAK activation can be monitored by GA treatment. The increase in PAP1 activity monitored after ABA or GA treatment might participate in the increase in PC level observed after 24 h hormone incubation
Fil: Villasuso, Ana Laura. Universidad Nacional de Rio Cuarto; Argentina
Fil: Di Palma, María Albana. Universidad Nacional de Rio Cuarto; Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina
Fil: Pasquaré, Susana J.. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina
Fil: Racagni, Graciela. Universidad Nacional de Rio Cuarto; Argentina
Fil: Giusto, Norma Maria. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina
Fil: Machado, Estela E.. Universidad Nacional de Rio Cuarto; Argentina
description Phosphatidic acid (PA) is the common lipid product in abscisic acid (ABA) and gibberellic acid (GA)response. In this work we investigated the lipid metabolism in response to both hormones. We could detect an in vivo phospholipase D activity (PLD, EC 3.1.4.4). This PLD produced [32P]PA (phosphatidic acid) rapidly (minutes) in the presence of ABA, confirming PA involvement in signal transduction, and transiently, indicating rapid PA removal after generation. The presence of PA removal by phosphatidate phosphatase 1 and 2 isoforms (E.C. 3.1.3.4) was verified in isolated aleurone membranes in vitro, the former but not the latter being Specifically responsive to the presence of GA or ABA. The in vitro DGPP phosphatase activity was not modified by short time incubation with GA or ABA while the in vitro PA kinase e that allows the production of 18:2-DGPP from 18:2-PA e is stimulated by ABA. The long term effects (24 h) of ABA or GA on lipid and fatty acid composition of aleurone layer cells were then investigated. An increase in PC and, to a lesser extent, in PE levels is the consequence of both hormone treatments. ABA, in aleurone layer cells, specifically activates a PLD whose product, PA, could be the substrate of PAP1 and/or PAK activities. Neither PLD nor PAK activation can be monitored by GA treatment. The increase in PAP1 activity monitored after ABA or GA treatment might participate in the increase in PC level observed after 24 h hormone incubation
publishDate 2013
dc.date.none.fl_str_mv 2013-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7393
Villasuso, Ana Laura; Di Palma, María Albana; Aveldaño, Marta Isabel; Pasquaré, Susana J.; Racagni, Graciela; et al.; Differences in phosphatidic acid signalling and metabolism between ABA and GA treatments of barley aleurone cells; Elsevier Masson; Plant Physiology And Biochemistry; 65; 2-2013; 1-8
0981-9428
url http://hdl.handle.net/11336/7393
identifier_str_mv Villasuso, Ana Laura; Di Palma, María Albana; Aveldaño, Marta Isabel; Pasquaré, Susana J.; Racagni, Graciela; et al.; Differences in phosphatidic acid signalling and metabolism between ABA and GA treatments of barley aleurone cells; Elsevier Masson; Plant Physiology And Biochemistry; 65; 2-2013; 1-8
0981-9428
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0981942813000247
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plaphy.2013.01.005
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/vnd.openxmlformats-officedocument.wordprocessingml.document
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Masson
publisher.none.fl_str_mv Elsevier Masson
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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