The surface organization of diacylglycerol pyrophosphate and its interaction with phosphatidic acid at the air-water interface

Autores
Villasuso, Ana Laura; Wilke, Natalia; Maggio, Bruno; Machado, Estela Edelmira
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Diacylglycerol pyrophosphate (DGPP), a phosphorylated form of phosphatidic acid (PA), gained attention recently due to its role as signaling lipid. However, little is known about its surface organization and potential impact on membrane-mediated function. In this work we investigated the interfacial behavior of Langmuir monolayers formed with pure DGPP and of its mixtures with PA. We found that changes of the subphase pH affect the surface behavior of DGPP. At pH 8, DGPP forms liquid expanded monolayers with a compressibility modulus of about 60 mN m−1 at collapse. On acidic solutions, the compressibility modulus increases to 90 mN m−1 and the average molecular area is smaller. At pH 8, DGPP and its precursor PA form thermodynamically favored topographically homogeneous non-ideal mixtures. The interaction among these lipids leads to a non-ideal diminution of the mean molecular area and consequently, to an increase of the compressibility modulus, with variations of the surface electrostatics. The favorable interaction of PA and DGPP, leading to changes of the film packing suggest that DGPP may act as a structural signal transducer in membrane-mediated cellular processes.
Fil: Villasuso, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Wilke, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Maggio, Bruno. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Machado, Estela Edelmira. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; Argentina
Materia
DIACYLGLYCEROL PYROPHOSPHATE
GLYCEROPHOSPHOLIPID MONOLAYERS
LIPID SIGNALING
MEMBRANE PACKING
PHOSPHATIDIC ACID
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/187611
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/187611
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The surface organization of diacylglycerol pyrophosphate and its interaction with phosphatidic acid at the air-water interfaceVillasuso, Ana LauraWilke, NataliaMaggio, BrunoMachado, Estela EdelmiraDIACYLGLYCEROL PYROPHOSPHATEGLYCEROPHOSPHOLIPID MONOLAYERSLIPID SIGNALINGMEMBRANE PACKINGPHOSPHATIDIC ACIDhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Diacylglycerol pyrophosphate (DGPP), a phosphorylated form of phosphatidic acid (PA), gained attention recently due to its role as signaling lipid. However, little is known about its surface organization and potential impact on membrane-mediated function. In this work we investigated the interfacial behavior of Langmuir monolayers formed with pure DGPP and of its mixtures with PA. We found that changes of the subphase pH affect the surface behavior of DGPP. At pH 8, DGPP forms liquid expanded monolayers with a compressibility modulus of about 60 mN m−1 at collapse. On acidic solutions, the compressibility modulus increases to 90 mN m−1 and the average molecular area is smaller. At pH 8, DGPP and its precursor PA form thermodynamically favored topographically homogeneous non-ideal mixtures. The interaction among these lipids leads to a non-ideal diminution of the mean molecular area and consequently, to an increase of the compressibility modulus, with variations of the surface electrostatics. The favorable interaction of PA and DGPP, leading to changes of the film packing suggest that DGPP may act as a structural signal transducer in membrane-mediated cellular processes.Fil: Villasuso, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Wilke, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Maggio, Bruno. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Machado, Estela Edelmira. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; ArgentinaElsevier Ireland2010-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/187611Villasuso, Ana Laura; Wilke, Natalia; Maggio, Bruno; Machado, Estela Edelmira; The surface organization of diacylglycerol pyrophosphate and its interaction with phosphatidic acid at the air-water interface; Elsevier Ireland; Chemistry and Physics of Lipids; 163; 8; 11-2010; 771-7770009-30841873-2941CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0009308410003439info:eu-repo/semantics/altIdentifier/doi/10.1016/j.chemphyslip.2010.09.002info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:44:55Zoai:ri.conicet.gov.ar:11336/187611instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:44:55.645CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The surface organization of diacylglycerol pyrophosphate and its interaction with phosphatidic acid at the air-water interface
title The surface organization of diacylglycerol pyrophosphate and its interaction with phosphatidic acid at the air-water interface
spellingShingle The surface organization of diacylglycerol pyrophosphate and its interaction with phosphatidic acid at the air-water interface
Villasuso, Ana Laura
DIACYLGLYCEROL PYROPHOSPHATE
GLYCEROPHOSPHOLIPID MONOLAYERS
LIPID SIGNALING
MEMBRANE PACKING
PHOSPHATIDIC ACID
title_short The surface organization of diacylglycerol pyrophosphate and its interaction with phosphatidic acid at the air-water interface
title_full The surface organization of diacylglycerol pyrophosphate and its interaction with phosphatidic acid at the air-water interface
title_fullStr The surface organization of diacylglycerol pyrophosphate and its interaction with phosphatidic acid at the air-water interface
title_full_unstemmed The surface organization of diacylglycerol pyrophosphate and its interaction with phosphatidic acid at the air-water interface
title_sort The surface organization of diacylglycerol pyrophosphate and its interaction with phosphatidic acid at the air-water interface
dc.creator.none.fl_str_mv Villasuso, Ana Laura
Wilke, Natalia
Maggio, Bruno
Machado, Estela Edelmira
author Villasuso, Ana Laura
author_facet Villasuso, Ana Laura
Wilke, Natalia
Maggio, Bruno
Machado, Estela Edelmira
author_role author
author2 Wilke, Natalia
Maggio, Bruno
Machado, Estela Edelmira
author2_role author
author
author
dc.subject.none.fl_str_mv DIACYLGLYCEROL PYROPHOSPHATE
GLYCEROPHOSPHOLIPID MONOLAYERS
LIPID SIGNALING
MEMBRANE PACKING
PHOSPHATIDIC ACID
topic DIACYLGLYCEROL PYROPHOSPHATE
GLYCEROPHOSPHOLIPID MONOLAYERS
LIPID SIGNALING
MEMBRANE PACKING
PHOSPHATIDIC ACID
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Diacylglycerol pyrophosphate (DGPP), a phosphorylated form of phosphatidic acid (PA), gained attention recently due to its role as signaling lipid. However, little is known about its surface organization and potential impact on membrane-mediated function. In this work we investigated the interfacial behavior of Langmuir monolayers formed with pure DGPP and of its mixtures with PA. We found that changes of the subphase pH affect the surface behavior of DGPP. At pH 8, DGPP forms liquid expanded monolayers with a compressibility modulus of about 60 mN m−1 at collapse. On acidic solutions, the compressibility modulus increases to 90 mN m−1 and the average molecular area is smaller. At pH 8, DGPP and its precursor PA form thermodynamically favored topographically homogeneous non-ideal mixtures. The interaction among these lipids leads to a non-ideal diminution of the mean molecular area and consequently, to an increase of the compressibility modulus, with variations of the surface electrostatics. The favorable interaction of PA and DGPP, leading to changes of the film packing suggest that DGPP may act as a structural signal transducer in membrane-mediated cellular processes.
Fil: Villasuso, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Wilke, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Maggio, Bruno. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Machado, Estela Edelmira. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; Argentina
description Diacylglycerol pyrophosphate (DGPP), a phosphorylated form of phosphatidic acid (PA), gained attention recently due to its role as signaling lipid. However, little is known about its surface organization and potential impact on membrane-mediated function. In this work we investigated the interfacial behavior of Langmuir monolayers formed with pure DGPP and of its mixtures with PA. We found that changes of the subphase pH affect the surface behavior of DGPP. At pH 8, DGPP forms liquid expanded monolayers with a compressibility modulus of about 60 mN m−1 at collapse. On acidic solutions, the compressibility modulus increases to 90 mN m−1 and the average molecular area is smaller. At pH 8, DGPP and its precursor PA form thermodynamically favored topographically homogeneous non-ideal mixtures. The interaction among these lipids leads to a non-ideal diminution of the mean molecular area and consequently, to an increase of the compressibility modulus, with variations of the surface electrostatics. The favorable interaction of PA and DGPP, leading to changes of the film packing suggest that DGPP may act as a structural signal transducer in membrane-mediated cellular processes.
publishDate 2010
dc.date.none.fl_str_mv 2010-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/187611
Villasuso, Ana Laura; Wilke, Natalia; Maggio, Bruno; Machado, Estela Edelmira; The surface organization of diacylglycerol pyrophosphate and its interaction with phosphatidic acid at the air-water interface; Elsevier Ireland; Chemistry and Physics of Lipids; 163; 8; 11-2010; 771-777
0009-3084
1873-2941
CONICET Digital
CONICET
url http://hdl.handle.net/11336/187611
identifier_str_mv Villasuso, Ana Laura; Wilke, Natalia; Maggio, Bruno; Machado, Estela Edelmira; The surface organization of diacylglycerol pyrophosphate and its interaction with phosphatidic acid at the air-water interface; Elsevier Ireland; Chemistry and Physics of Lipids; 163; 8; 11-2010; 771-777
0009-3084
1873-2941
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0009308410003439
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.chemphyslip.2010.09.002
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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