Canine distemper virus, atypical toxoplasma gondii, and neospora caninum co-infection, in a dog with neurological signs from Argentina

Autores
Postma, Gabriela; Dellarupe, Andrea; Streitenberger, Nicolás; Bratanich, Ana Cristina; Venturini, María Cecilia; Minatel, Leonardo
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The occurrence of combined infections by Toxoplasma gondii, Neospora caninum and Canine distemper virus (CDV) in domestic dogs and wildlife animals has not been frequently reported, and the histopathological findings were not exhaustively described. The objective of this study was to report a co-infection of CDV, T. gondii and N. caninum in a dog with neurological signs, as well as the molecular characterization of the protozoa involved. A young street dog was rescued with neurological clinical signs and died spontaneously. A complete necropsy was performed. Tissues were collected and fixed for histopathological evaluation. Additionally, sections of the central nervous system (CNS) and heart were assayed by immunohistochemistry (IHQ) for T. gondii and N. caninum. Sample of brain tissue was analyzed by PCR and nPCR-RFLP for T. gondii genotyping. Spleen was used for detection of CDV by RT-PCR. Gross lesions were not observed, with the exception of the lung. Microscopically, a severe necrosuppurative meningoencephalitis with vasculitis, tachyzoites and bradyzoites of T. gondii and N. caninum were found. Demyelination was also evident, associated with eosinophilic intranuclear inclusion bodies within astrocytes. CDV was PCR positive while both parasites were presented PCR and IHQ positive results. Molecular characterization of T. gondii was reported as atypical #14 (likely). To our knowledge, this is the first report of genetical identification of T. gondii obtained from the brain of a naturally infected dog in Argentina. The results emphasize the importance of different techniques as diagnostic tools to enhance the detection of causative agents in cases of fatal encephalitis.
Fil: Postma, Gabriela. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Dellarupe, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Streitenberger, Nicolás. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Cátedra de Patología Especial Veterinaria; Argentina
Fil: Bratanich, Ana Cristina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Venturini, María Cecilia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Minatel, Leonardo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Materia
CANINE DISTEMPER VIRUS
GENOTYPING
NEOSPORA CANINUM
TOXOPLASMA GONDII
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/131046
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/131046
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Canine distemper virus, atypical toxoplasma gondii, and neospora caninum co-infection, in a dog with neurological signs from ArgentinaPostma, GabrielaDellarupe, AndreaStreitenberger, NicolásBratanich, Ana CristinaVenturini, María CeciliaMinatel, LeonardoCANINE DISTEMPER VIRUSGENOTYPINGNEOSPORA CANINUMTOXOPLASMA GONDIIhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The occurrence of combined infections by Toxoplasma gondii, Neospora caninum and Canine distemper virus (CDV) in domestic dogs and wildlife animals has not been frequently reported, and the histopathological findings were not exhaustively described. The objective of this study was to report a co-infection of CDV, T. gondii and N. caninum in a dog with neurological signs, as well as the molecular characterization of the protozoa involved. A young street dog was rescued with neurological clinical signs and died spontaneously. A complete necropsy was performed. Tissues were collected and fixed for histopathological evaluation. Additionally, sections of the central nervous system (CNS) and heart were assayed by immunohistochemistry (IHQ) for T. gondii and N. caninum. Sample of brain tissue was analyzed by PCR and nPCR-RFLP for T. gondii genotyping. Spleen was used for detection of CDV by RT-PCR. Gross lesions were not observed, with the exception of the lung. Microscopically, a severe necrosuppurative meningoencephalitis with vasculitis, tachyzoites and bradyzoites of T. gondii and N. caninum were found. Demyelination was also evident, associated with eosinophilic intranuclear inclusion bodies within astrocytes. CDV was PCR positive while both parasites were presented PCR and IHQ positive results. Molecular characterization of T. gondii was reported as atypical #14 (likely). To our knowledge, this is the first report of genetical identification of T. gondii obtained from the brain of a naturally infected dog in Argentina. The results emphasize the importance of different techniques as diagnostic tools to enhance the detection of causative agents in cases of fatal encephalitis.Fil: Postma, Gabriela. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Dellarupe, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; ArgentinaFil: Streitenberger, Nicolás. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Cátedra de Patología Especial Veterinaria; ArgentinaFil: Bratanich, Ana Cristina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Venturini, María Cecilia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; ArgentinaFil: Minatel, Leonardo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaBrazilian Association of Veterinary Pathology2019-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/131046Postma, Gabriela; Dellarupe, Andrea; Streitenberger, Nicolás; Bratanich, Ana Cristina; Venturini, María Cecilia; et al.; Canine distemper virus, atypical toxoplasma gondii, and neospora caninum co-infection, in a dog with neurological signs from Argentina; Brazilian Association of Veterinary Pathology; Brazilian Journal of Veterinary Pathology; 12; 3; 11-2019; 101-1051983-0246CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.24070/bjvp.1983-0246.v12i3p101-105info:eu-repo/semantics/altIdentifier/url/https://pesquisa.bvsalud.org/portal/resource/pt/vti-24439info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:38Zoai:ri.conicet.gov.ar:11336/131046instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:38.291CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Canine distemper virus, atypical toxoplasma gondii, and neospora caninum co-infection, in a dog with neurological signs from Argentina
title Canine distemper virus, atypical toxoplasma gondii, and neospora caninum co-infection, in a dog with neurological signs from Argentina
spellingShingle Canine distemper virus, atypical toxoplasma gondii, and neospora caninum co-infection, in a dog with neurological signs from Argentina
Postma, Gabriela
CANINE DISTEMPER VIRUS
GENOTYPING
NEOSPORA CANINUM
TOXOPLASMA GONDII
title_short Canine distemper virus, atypical toxoplasma gondii, and neospora caninum co-infection, in a dog with neurological signs from Argentina
title_full Canine distemper virus, atypical toxoplasma gondii, and neospora caninum co-infection, in a dog with neurological signs from Argentina
title_fullStr Canine distemper virus, atypical toxoplasma gondii, and neospora caninum co-infection, in a dog with neurological signs from Argentina
title_full_unstemmed Canine distemper virus, atypical toxoplasma gondii, and neospora caninum co-infection, in a dog with neurological signs from Argentina
title_sort Canine distemper virus, atypical toxoplasma gondii, and neospora caninum co-infection, in a dog with neurological signs from Argentina
dc.creator.none.fl_str_mv Postma, Gabriela
Dellarupe, Andrea
Streitenberger, Nicolás
Bratanich, Ana Cristina
Venturini, María Cecilia
Minatel, Leonardo
author Postma, Gabriela
author_facet Postma, Gabriela
Dellarupe, Andrea
Streitenberger, Nicolás
Bratanich, Ana Cristina
Venturini, María Cecilia
Minatel, Leonardo
author_role author
author2 Dellarupe, Andrea
Streitenberger, Nicolás
Bratanich, Ana Cristina
Venturini, María Cecilia
Minatel, Leonardo
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CANINE DISTEMPER VIRUS
GENOTYPING
NEOSPORA CANINUM
TOXOPLASMA GONDII
topic CANINE DISTEMPER VIRUS
GENOTYPING
NEOSPORA CANINUM
TOXOPLASMA GONDII
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The occurrence of combined infections by Toxoplasma gondii, Neospora caninum and Canine distemper virus (CDV) in domestic dogs and wildlife animals has not been frequently reported, and the histopathological findings were not exhaustively described. The objective of this study was to report a co-infection of CDV, T. gondii and N. caninum in a dog with neurological signs, as well as the molecular characterization of the protozoa involved. A young street dog was rescued with neurological clinical signs and died spontaneously. A complete necropsy was performed. Tissues were collected and fixed for histopathological evaluation. Additionally, sections of the central nervous system (CNS) and heart were assayed by immunohistochemistry (IHQ) for T. gondii and N. caninum. Sample of brain tissue was analyzed by PCR and nPCR-RFLP for T. gondii genotyping. Spleen was used for detection of CDV by RT-PCR. Gross lesions were not observed, with the exception of the lung. Microscopically, a severe necrosuppurative meningoencephalitis with vasculitis, tachyzoites and bradyzoites of T. gondii and N. caninum were found. Demyelination was also evident, associated with eosinophilic intranuclear inclusion bodies within astrocytes. CDV was PCR positive while both parasites were presented PCR and IHQ positive results. Molecular characterization of T. gondii was reported as atypical #14 (likely). To our knowledge, this is the first report of genetical identification of T. gondii obtained from the brain of a naturally infected dog in Argentina. The results emphasize the importance of different techniques as diagnostic tools to enhance the detection of causative agents in cases of fatal encephalitis.
Fil: Postma, Gabriela. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Dellarupe, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Streitenberger, Nicolás. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Cátedra de Patología Especial Veterinaria; Argentina
Fil: Bratanich, Ana Cristina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Venturini, María Cecilia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Minatel, Leonardo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
description The occurrence of combined infections by Toxoplasma gondii, Neospora caninum and Canine distemper virus (CDV) in domestic dogs and wildlife animals has not been frequently reported, and the histopathological findings were not exhaustively described. The objective of this study was to report a co-infection of CDV, T. gondii and N. caninum in a dog with neurological signs, as well as the molecular characterization of the protozoa involved. A young street dog was rescued with neurological clinical signs and died spontaneously. A complete necropsy was performed. Tissues were collected and fixed for histopathological evaluation. Additionally, sections of the central nervous system (CNS) and heart were assayed by immunohistochemistry (IHQ) for T. gondii and N. caninum. Sample of brain tissue was analyzed by PCR and nPCR-RFLP for T. gondii genotyping. Spleen was used for detection of CDV by RT-PCR. Gross lesions were not observed, with the exception of the lung. Microscopically, a severe necrosuppurative meningoencephalitis with vasculitis, tachyzoites and bradyzoites of T. gondii and N. caninum were found. Demyelination was also evident, associated with eosinophilic intranuclear inclusion bodies within astrocytes. CDV was PCR positive while both parasites were presented PCR and IHQ positive results. Molecular characterization of T. gondii was reported as atypical #14 (likely). To our knowledge, this is the first report of genetical identification of T. gondii obtained from the brain of a naturally infected dog in Argentina. The results emphasize the importance of different techniques as diagnostic tools to enhance the detection of causative agents in cases of fatal encephalitis.
publishDate 2019
dc.date.none.fl_str_mv 2019-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/131046
Postma, Gabriela; Dellarupe, Andrea; Streitenberger, Nicolás; Bratanich, Ana Cristina; Venturini, María Cecilia; et al.; Canine distemper virus, atypical toxoplasma gondii, and neospora caninum co-infection, in a dog with neurological signs from Argentina; Brazilian Association of Veterinary Pathology; Brazilian Journal of Veterinary Pathology; 12; 3; 11-2019; 101-105
1983-0246
CONICET Digital
CONICET
url http://hdl.handle.net/11336/131046
identifier_str_mv Postma, Gabriela; Dellarupe, Andrea; Streitenberger, Nicolás; Bratanich, Ana Cristina; Venturini, María Cecilia; et al.; Canine distemper virus, atypical toxoplasma gondii, and neospora caninum co-infection, in a dog with neurological signs from Argentina; Brazilian Association of Veterinary Pathology; Brazilian Journal of Veterinary Pathology; 12; 3; 11-2019; 101-105
1983-0246
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.24070/bjvp.1983-0246.v12i3p101-105
info:eu-repo/semantics/altIdentifier/url/https://pesquisa.bvsalud.org/portal/resource/pt/vti-24439
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Brazilian Association of Veterinary Pathology
publisher.none.fl_str_mv Brazilian Association of Veterinary Pathology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269532212690944
score 13.13397

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