Chronic Protein Restriction in Mice Impacts Placental Function and Maternal Body Weight before Fetal Growth
- Autores
- Gonzalez, Paula Natalia; Gasperowicz, Malgorzata; Barbeito Andrés, Jimena; Klenin, Natasha; Cross, James C.; Hallgrimsson, Benedikt
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Mechanisms of resource allocation are essential for maternal and fetal survival, particularly when the availability of nutrients is limited. We investigated the responses of feto-placental development to maternal chronic protein malnutrition to test the hypothesis that maternal low protein diet produces differential growth restriction of placental and fetal tissues, and adaptive changes in the placenta that may mitigate impacts on fetal growth. C57BL/6J female mice were fed either a low-protein diet (6% protein) or control isocaloric diet (20% protein). On embryonic days E10.5, 17.5 and 18.5 tissue samples were prepared for morphometric, histological and quantitative RT-PCR analyses, which included markers of trophoblast cell subtypes. Potential endocrine adaptations were assessed by the expression of Prolactin-related hormone genes. In the low protein group, placenta weight was significantly lower at E10.5, followed by reduction of maternal weight at E17.5, while the fetuses became significantly lighter no earlier than at E18.5. Fetal head at E18.5 in the low protein group, though smaller than controls, was larger than expected for body size. The relative size and shape of the cranial vault and the flexion of the cranial base was affected by E17.5 and more severely by E18.5. The junctional zone, a placenta layer rich in endocrine and energy storing glycogen cells, was smaller in low protein placentas as well as the expression of Pcdh12, a marker of glycogen trophoblast cells. Placental hormone gene Prl3a1 was altered in response to low protein diet: expression was elevated at E17.5 when fetuses were still growing normally, but dropped sharply by E18.5 in parallel with the slowing of fetal growth. This model suggests that nutrients are preferentially allocated to sustain fetal and brain growth and suggests the placenta as a nutrient sensor in early gestation with a role in mitigating impacts of poor maternal nutrition on fetal growth.
Fil: Gonzalez, Paula Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria ; Argentina
Fil: Gasperowicz, Malgorzata. University of Calgary; Canadá
Fil: Barbeito Andrés, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria ; Argentina
Fil: Klenin, Natasha. University of Calgary; Canadá
Fil: Cross, James C.. University of Calgary; Canadá
Fil: Hallgrimsson, Benedikt. University of Calgary; Canadá - Materia
-
MATERNAL UNDERNUTRITION
BRAIN SPARING
RESOURCE ALLOCATION - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/48523
Ver los metadatos del registro completo
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CONICET Digital (CONICET) |
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Chronic Protein Restriction in Mice Impacts Placental Function and Maternal Body Weight before Fetal GrowthGonzalez, Paula NataliaGasperowicz, MalgorzataBarbeito Andrés, JimenaKlenin, NatashaCross, James C.Hallgrimsson, BenediktMATERNAL UNDERNUTRITIONBRAIN SPARINGRESOURCE ALLOCATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Mechanisms of resource allocation are essential for maternal and fetal survival, particularly when the availability of nutrients is limited. We investigated the responses of feto-placental development to maternal chronic protein malnutrition to test the hypothesis that maternal low protein diet produces differential growth restriction of placental and fetal tissues, and adaptive changes in the placenta that may mitigate impacts on fetal growth. C57BL/6J female mice were fed either a low-protein diet (6% protein) or control isocaloric diet (20% protein). On embryonic days E10.5, 17.5 and 18.5 tissue samples were prepared for morphometric, histological and quantitative RT-PCR analyses, which included markers of trophoblast cell subtypes. Potential endocrine adaptations were assessed by the expression of Prolactin-related hormone genes. In the low protein group, placenta weight was significantly lower at E10.5, followed by reduction of maternal weight at E17.5, while the fetuses became significantly lighter no earlier than at E18.5. Fetal head at E18.5 in the low protein group, though smaller than controls, was larger than expected for body size. The relative size and shape of the cranial vault and the flexion of the cranial base was affected by E17.5 and more severely by E18.5. The junctional zone, a placenta layer rich in endocrine and energy storing glycogen cells, was smaller in low protein placentas as well as the expression of Pcdh12, a marker of glycogen trophoblast cells. Placental hormone gene Prl3a1 was altered in response to low protein diet: expression was elevated at E17.5 when fetuses were still growing normally, but dropped sharply by E18.5 in parallel with the slowing of fetal growth. This model suggests that nutrients are preferentially allocated to sustain fetal and brain growth and suggests the placenta as a nutrient sensor in early gestation with a role in mitigating impacts of poor maternal nutrition on fetal growth.Fil: Gonzalez, Paula Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria ; ArgentinaFil: Gasperowicz, Malgorzata. University of Calgary; CanadáFil: Barbeito Andrés, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria ; ArgentinaFil: Klenin, Natasha. University of Calgary; CanadáFil: Cross, James C.. University of Calgary; CanadáFil: Hallgrimsson, Benedikt. University of Calgary; CanadáPublic Library of Science2016-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/48523Gonzalez, Paula Natalia; Gasperowicz, Malgorzata; Barbeito Andrés, Jimena; Klenin, Natasha; Cross, James C.; et al.; Chronic Protein Restriction in Mice Impacts Placental Function and Maternal Body Weight before Fetal Growth; Public Library of Science; Plos One; 11; 3; 3-2016; 1-18; e01522271932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0152227info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0152227info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:10:25Zoai:ri.conicet.gov.ar:11336/48523instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:10:25.451CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Chronic Protein Restriction in Mice Impacts Placental Function and Maternal Body Weight before Fetal Growth |
title |
Chronic Protein Restriction in Mice Impacts Placental Function and Maternal Body Weight before Fetal Growth |
spellingShingle |
Chronic Protein Restriction in Mice Impacts Placental Function and Maternal Body Weight before Fetal Growth Gonzalez, Paula Natalia MATERNAL UNDERNUTRITION BRAIN SPARING RESOURCE ALLOCATION |
title_short |
Chronic Protein Restriction in Mice Impacts Placental Function and Maternal Body Weight before Fetal Growth |
title_full |
Chronic Protein Restriction in Mice Impacts Placental Function and Maternal Body Weight before Fetal Growth |
title_fullStr |
Chronic Protein Restriction in Mice Impacts Placental Function and Maternal Body Weight before Fetal Growth |
title_full_unstemmed |
Chronic Protein Restriction in Mice Impacts Placental Function and Maternal Body Weight before Fetal Growth |
title_sort |
Chronic Protein Restriction in Mice Impacts Placental Function and Maternal Body Weight before Fetal Growth |
dc.creator.none.fl_str_mv |
Gonzalez, Paula Natalia Gasperowicz, Malgorzata Barbeito Andrés, Jimena Klenin, Natasha Cross, James C. Hallgrimsson, Benedikt |
author |
Gonzalez, Paula Natalia |
author_facet |
Gonzalez, Paula Natalia Gasperowicz, Malgorzata Barbeito Andrés, Jimena Klenin, Natasha Cross, James C. Hallgrimsson, Benedikt |
author_role |
author |
author2 |
Gasperowicz, Malgorzata Barbeito Andrés, Jimena Klenin, Natasha Cross, James C. Hallgrimsson, Benedikt |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
MATERNAL UNDERNUTRITION BRAIN SPARING RESOURCE ALLOCATION |
topic |
MATERNAL UNDERNUTRITION BRAIN SPARING RESOURCE ALLOCATION |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Mechanisms of resource allocation are essential for maternal and fetal survival, particularly when the availability of nutrients is limited. We investigated the responses of feto-placental development to maternal chronic protein malnutrition to test the hypothesis that maternal low protein diet produces differential growth restriction of placental and fetal tissues, and adaptive changes in the placenta that may mitigate impacts on fetal growth. C57BL/6J female mice were fed either a low-protein diet (6% protein) or control isocaloric diet (20% protein). On embryonic days E10.5, 17.5 and 18.5 tissue samples were prepared for morphometric, histological and quantitative RT-PCR analyses, which included markers of trophoblast cell subtypes. Potential endocrine adaptations were assessed by the expression of Prolactin-related hormone genes. In the low protein group, placenta weight was significantly lower at E10.5, followed by reduction of maternal weight at E17.5, while the fetuses became significantly lighter no earlier than at E18.5. Fetal head at E18.5 in the low protein group, though smaller than controls, was larger than expected for body size. The relative size and shape of the cranial vault and the flexion of the cranial base was affected by E17.5 and more severely by E18.5. The junctional zone, a placenta layer rich in endocrine and energy storing glycogen cells, was smaller in low protein placentas as well as the expression of Pcdh12, a marker of glycogen trophoblast cells. Placental hormone gene Prl3a1 was altered in response to low protein diet: expression was elevated at E17.5 when fetuses were still growing normally, but dropped sharply by E18.5 in parallel with the slowing of fetal growth. This model suggests that nutrients are preferentially allocated to sustain fetal and brain growth and suggests the placenta as a nutrient sensor in early gestation with a role in mitigating impacts of poor maternal nutrition on fetal growth. Fil: Gonzalez, Paula Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria ; Argentina Fil: Gasperowicz, Malgorzata. University of Calgary; Canadá Fil: Barbeito Andrés, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria ; Argentina Fil: Klenin, Natasha. University of Calgary; Canadá Fil: Cross, James C.. University of Calgary; Canadá Fil: Hallgrimsson, Benedikt. University of Calgary; Canadá |
description |
Mechanisms of resource allocation are essential for maternal and fetal survival, particularly when the availability of nutrients is limited. We investigated the responses of feto-placental development to maternal chronic protein malnutrition to test the hypothesis that maternal low protein diet produces differential growth restriction of placental and fetal tissues, and adaptive changes in the placenta that may mitigate impacts on fetal growth. C57BL/6J female mice were fed either a low-protein diet (6% protein) or control isocaloric diet (20% protein). On embryonic days E10.5, 17.5 and 18.5 tissue samples were prepared for morphometric, histological and quantitative RT-PCR analyses, which included markers of trophoblast cell subtypes. Potential endocrine adaptations were assessed by the expression of Prolactin-related hormone genes. In the low protein group, placenta weight was significantly lower at E10.5, followed by reduction of maternal weight at E17.5, while the fetuses became significantly lighter no earlier than at E18.5. Fetal head at E18.5 in the low protein group, though smaller than controls, was larger than expected for body size. The relative size and shape of the cranial vault and the flexion of the cranial base was affected by E17.5 and more severely by E18.5. The junctional zone, a placenta layer rich in endocrine and energy storing glycogen cells, was smaller in low protein placentas as well as the expression of Pcdh12, a marker of glycogen trophoblast cells. Placental hormone gene Prl3a1 was altered in response to low protein diet: expression was elevated at E17.5 when fetuses were still growing normally, but dropped sharply by E18.5 in parallel with the slowing of fetal growth. This model suggests that nutrients are preferentially allocated to sustain fetal and brain growth and suggests the placenta as a nutrient sensor in early gestation with a role in mitigating impacts of poor maternal nutrition on fetal growth. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-03 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/48523 Gonzalez, Paula Natalia; Gasperowicz, Malgorzata; Barbeito Andrés, Jimena; Klenin, Natasha; Cross, James C.; et al.; Chronic Protein Restriction in Mice Impacts Placental Function and Maternal Body Weight before Fetal Growth; Public Library of Science; Plos One; 11; 3; 3-2016; 1-18; e0152227 1932-6203 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/48523 |
identifier_str_mv |
Gonzalez, Paula Natalia; Gasperowicz, Malgorzata; Barbeito Andrés, Jimena; Klenin, Natasha; Cross, James C.; et al.; Chronic Protein Restriction in Mice Impacts Placental Function and Maternal Body Weight before Fetal Growth; Public Library of Science; Plos One; 11; 3; 3-2016; 1-18; e0152227 1932-6203 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0152227 info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0152227 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Public Library of Science |
publisher.none.fl_str_mv |
Public Library of Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613993359998976 |
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13.070432 |