Chronic Protein Restriction in Mice Impacts Placental Function and Maternal Body Weight before Fetal Growth

Autores
Gonzalez, Paula Natalia; Gasperowicz, Malgorzata; Barbeito Andrés, Jimena; Klenin, Natasha; Cross, James C.; Hallgrimsson, Benedikt
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Mechanisms of resource allocation are essential for maternal and fetal survival, particularly when the availability of nutrients is limited. We investigated the responses of feto-placental development to maternal chronic protein malnutrition to test the hypothesis that maternal low protein diet produces differential growth restriction of placental and fetal tissues, and adaptive changes in the placenta that may mitigate impacts on fetal growth. C57BL/6J female mice were fed either a low-protein diet (6% protein) or control isocaloric diet (20% protein). On embryonic days E10.5, 17.5 and 18.5 tissue samples were prepared for morphometric, histological and quantitative RT-PCR analyses, which included markers of trophoblast cell subtypes. Potential endocrine adaptations were assessed by the expression of Prolactin-related hormone genes. In the low protein group, placenta weight was significantly lower at E10.5, followed by reduction of maternal weight at E17.5, while the fetuses became significantly lighter no earlier than at E18.5. Fetal head at E18.5 in the low protein group, though smaller than controls, was larger than expected for body size. The relative size and shape of the cranial vault and the flexion of the cranial base was affected by E17.5 and more severely by E18.5. The junctional zone, a placenta layer rich in endocrine and energy storing glycogen cells, was smaller in low protein placentas as well as the expression of Pcdh12, a marker of glycogen trophoblast cells. Placental hormone gene Prl3a1 was altered in response to low protein diet: expression was elevated at E17.5 when fetuses were still growing normally, but dropped sharply by E18.5 in parallel with the slowing of fetal growth. This model suggests that nutrients are preferentially allocated to sustain fetal and brain growth and suggests the placenta as a nutrient sensor in early gestation with a role in mitigating impacts of poor maternal nutrition on fetal growth.
Fil: Gonzalez, Paula Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria ; Argentina
Fil: Gasperowicz, Malgorzata. University of Calgary; Canadá
Fil: Barbeito Andrés, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria ; Argentina
Fil: Klenin, Natasha. University of Calgary; Canadá
Fil: Cross, James C.. University of Calgary; Canadá
Fil: Hallgrimsson, Benedikt. University of Calgary; Canadá
Materia
MATERNAL UNDERNUTRITION
BRAIN SPARING
RESOURCE ALLOCATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/48523

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network_name_str CONICET Digital (CONICET)
spelling Chronic Protein Restriction in Mice Impacts Placental Function and Maternal Body Weight before Fetal GrowthGonzalez, Paula NataliaGasperowicz, MalgorzataBarbeito Andrés, JimenaKlenin, NatashaCross, James C.Hallgrimsson, BenediktMATERNAL UNDERNUTRITIONBRAIN SPARINGRESOURCE ALLOCATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Mechanisms of resource allocation are essential for maternal and fetal survival, particularly when the availability of nutrients is limited. We investigated the responses of feto-placental development to maternal chronic protein malnutrition to test the hypothesis that maternal low protein diet produces differential growth restriction of placental and fetal tissues, and adaptive changes in the placenta that may mitigate impacts on fetal growth. C57BL/6J female mice were fed either a low-protein diet (6% protein) or control isocaloric diet (20% protein). On embryonic days E10.5, 17.5 and 18.5 tissue samples were prepared for morphometric, histological and quantitative RT-PCR analyses, which included markers of trophoblast cell subtypes. Potential endocrine adaptations were assessed by the expression of Prolactin-related hormone genes. In the low protein group, placenta weight was significantly lower at E10.5, followed by reduction of maternal weight at E17.5, while the fetuses became significantly lighter no earlier than at E18.5. Fetal head at E18.5 in the low protein group, though smaller than controls, was larger than expected for body size. The relative size and shape of the cranial vault and the flexion of the cranial base was affected by E17.5 and more severely by E18.5. The junctional zone, a placenta layer rich in endocrine and energy storing glycogen cells, was smaller in low protein placentas as well as the expression of Pcdh12, a marker of glycogen trophoblast cells. Placental hormone gene Prl3a1 was altered in response to low protein diet: expression was elevated at E17.5 when fetuses were still growing normally, but dropped sharply by E18.5 in parallel with the slowing of fetal growth. This model suggests that nutrients are preferentially allocated to sustain fetal and brain growth and suggests the placenta as a nutrient sensor in early gestation with a role in mitigating impacts of poor maternal nutrition on fetal growth.Fil: Gonzalez, Paula Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria ; ArgentinaFil: Gasperowicz, Malgorzata. University of Calgary; CanadáFil: Barbeito Andrés, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria ; ArgentinaFil: Klenin, Natasha. University of Calgary; CanadáFil: Cross, James C.. University of Calgary; CanadáFil: Hallgrimsson, Benedikt. University of Calgary; CanadáPublic Library of Science2016-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/48523Gonzalez, Paula Natalia; Gasperowicz, Malgorzata; Barbeito Andrés, Jimena; Klenin, Natasha; Cross, James C.; et al.; Chronic Protein Restriction in Mice Impacts Placental Function and Maternal Body Weight before Fetal Growth; Public Library of Science; Plos One; 11; 3; 3-2016; 1-18; e01522271932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0152227info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0152227info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:10:25Zoai:ri.conicet.gov.ar:11336/48523instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:10:25.451CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Chronic Protein Restriction in Mice Impacts Placental Function and Maternal Body Weight before Fetal Growth
title Chronic Protein Restriction in Mice Impacts Placental Function and Maternal Body Weight before Fetal Growth
spellingShingle Chronic Protein Restriction in Mice Impacts Placental Function and Maternal Body Weight before Fetal Growth
Gonzalez, Paula Natalia
MATERNAL UNDERNUTRITION
BRAIN SPARING
RESOURCE ALLOCATION
title_short Chronic Protein Restriction in Mice Impacts Placental Function and Maternal Body Weight before Fetal Growth
title_full Chronic Protein Restriction in Mice Impacts Placental Function and Maternal Body Weight before Fetal Growth
title_fullStr Chronic Protein Restriction in Mice Impacts Placental Function and Maternal Body Weight before Fetal Growth
title_full_unstemmed Chronic Protein Restriction in Mice Impacts Placental Function and Maternal Body Weight before Fetal Growth
title_sort Chronic Protein Restriction in Mice Impacts Placental Function and Maternal Body Weight before Fetal Growth
dc.creator.none.fl_str_mv Gonzalez, Paula Natalia
Gasperowicz, Malgorzata
Barbeito Andrés, Jimena
Klenin, Natasha
Cross, James C.
Hallgrimsson, Benedikt
author Gonzalez, Paula Natalia
author_facet Gonzalez, Paula Natalia
Gasperowicz, Malgorzata
Barbeito Andrés, Jimena
Klenin, Natasha
Cross, James C.
Hallgrimsson, Benedikt
author_role author
author2 Gasperowicz, Malgorzata
Barbeito Andrés, Jimena
Klenin, Natasha
Cross, James C.
Hallgrimsson, Benedikt
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv MATERNAL UNDERNUTRITION
BRAIN SPARING
RESOURCE ALLOCATION
topic MATERNAL UNDERNUTRITION
BRAIN SPARING
RESOURCE ALLOCATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Mechanisms of resource allocation are essential for maternal and fetal survival, particularly when the availability of nutrients is limited. We investigated the responses of feto-placental development to maternal chronic protein malnutrition to test the hypothesis that maternal low protein diet produces differential growth restriction of placental and fetal tissues, and adaptive changes in the placenta that may mitigate impacts on fetal growth. C57BL/6J female mice were fed either a low-protein diet (6% protein) or control isocaloric diet (20% protein). On embryonic days E10.5, 17.5 and 18.5 tissue samples were prepared for morphometric, histological and quantitative RT-PCR analyses, which included markers of trophoblast cell subtypes. Potential endocrine adaptations were assessed by the expression of Prolactin-related hormone genes. In the low protein group, placenta weight was significantly lower at E10.5, followed by reduction of maternal weight at E17.5, while the fetuses became significantly lighter no earlier than at E18.5. Fetal head at E18.5 in the low protein group, though smaller than controls, was larger than expected for body size. The relative size and shape of the cranial vault and the flexion of the cranial base was affected by E17.5 and more severely by E18.5. The junctional zone, a placenta layer rich in endocrine and energy storing glycogen cells, was smaller in low protein placentas as well as the expression of Pcdh12, a marker of glycogen trophoblast cells. Placental hormone gene Prl3a1 was altered in response to low protein diet: expression was elevated at E17.5 when fetuses were still growing normally, but dropped sharply by E18.5 in parallel with the slowing of fetal growth. This model suggests that nutrients are preferentially allocated to sustain fetal and brain growth and suggests the placenta as a nutrient sensor in early gestation with a role in mitigating impacts of poor maternal nutrition on fetal growth.
Fil: Gonzalez, Paula Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria ; Argentina
Fil: Gasperowicz, Malgorzata. University of Calgary; Canadá
Fil: Barbeito Andrés, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria ; Argentina
Fil: Klenin, Natasha. University of Calgary; Canadá
Fil: Cross, James C.. University of Calgary; Canadá
Fil: Hallgrimsson, Benedikt. University of Calgary; Canadá
description Mechanisms of resource allocation are essential for maternal and fetal survival, particularly when the availability of nutrients is limited. We investigated the responses of feto-placental development to maternal chronic protein malnutrition to test the hypothesis that maternal low protein diet produces differential growth restriction of placental and fetal tissues, and adaptive changes in the placenta that may mitigate impacts on fetal growth. C57BL/6J female mice were fed either a low-protein diet (6% protein) or control isocaloric diet (20% protein). On embryonic days E10.5, 17.5 and 18.5 tissue samples were prepared for morphometric, histological and quantitative RT-PCR analyses, which included markers of trophoblast cell subtypes. Potential endocrine adaptations were assessed by the expression of Prolactin-related hormone genes. In the low protein group, placenta weight was significantly lower at E10.5, followed by reduction of maternal weight at E17.5, while the fetuses became significantly lighter no earlier than at E18.5. Fetal head at E18.5 in the low protein group, though smaller than controls, was larger than expected for body size. The relative size and shape of the cranial vault and the flexion of the cranial base was affected by E17.5 and more severely by E18.5. The junctional zone, a placenta layer rich in endocrine and energy storing glycogen cells, was smaller in low protein placentas as well as the expression of Pcdh12, a marker of glycogen trophoblast cells. Placental hormone gene Prl3a1 was altered in response to low protein diet: expression was elevated at E17.5 when fetuses were still growing normally, but dropped sharply by E18.5 in parallel with the slowing of fetal growth. This model suggests that nutrients are preferentially allocated to sustain fetal and brain growth and suggests the placenta as a nutrient sensor in early gestation with a role in mitigating impacts of poor maternal nutrition on fetal growth.
publishDate 2016
dc.date.none.fl_str_mv 2016-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/48523
Gonzalez, Paula Natalia; Gasperowicz, Malgorzata; Barbeito Andrés, Jimena; Klenin, Natasha; Cross, James C.; et al.; Chronic Protein Restriction in Mice Impacts Placental Function and Maternal Body Weight before Fetal Growth; Public Library of Science; Plos One; 11; 3; 3-2016; 1-18; e0152227
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/48523
identifier_str_mv Gonzalez, Paula Natalia; Gasperowicz, Malgorzata; Barbeito Andrés, Jimena; Klenin, Natasha; Cross, James C.; et al.; Chronic Protein Restriction in Mice Impacts Placental Function and Maternal Body Weight before Fetal Growth; Public Library of Science; Plos One; 11; 3; 3-2016; 1-18; e0152227
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0152227
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0152227
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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