A Novel, Quick, and Reliable Smartphone-Based Method for Serum PSA Quantification: Original Design of a Portable Microfluidic Immunosensor-Based System

Autores
Ortega Sánchez, Francisco Gabriel; Gómez, Carlos Germán; González Martinez, Coral; Valero, Teresa; Expósito Hernández, José; Pucheta, Pablo Ignacio; Rodriguez Martinez, Alba; Serrano, María José; Lorente, José Antonio; Fernández Baldo, Martín Alejandro
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We describe a versatile, portable, and simple platform that includes a microfluidic electrochemical immunosensor for prostate-specific antigen (PSA) detection. It is based on the covalent immobilization of the anti-PSA monoclonal antibody on magnetic microbeads retained in the central channel of a microfluidic device. Image flow cytometry and scanning electron microscopy were used to characterize the magnetic microbeads. A direct sandwich immunoassay (with horseradish peroxidase-conjugated PSA antibody) served to quantify the cancer biomarker in serum samples. The enzymatic product was detected at −100 mV by amperometry on sputtered thin-film electrodes. Electrochemical reaction produced a current proportional to the PSA level, with a linear range from 10 pg mL−1 to 1500 pg mL−1. The sensitivity was demonstrated by a detection limit of 2 pg mL−1 and the reproducibility by a coefficient of variation of 6.16%. The clinical performance of this platform was tested in serum samples from patients with prostate cancer (PCa), observing high specificity and full correlation with gold standard determinations. In conclusion, this analytical platform is a promising tool for measuring PSA levels in patients with PCa, offering a high sensitivity and reduced variability. The small platform size and low cost of this quantitative methodology support its suitability for the fast and sensitive analysis of PSA and other circulating biomarkers in patients. Further research is warranted to verify these findings and explore its potential application at all healthcare levels.
Fil: Ortega Sánchez, Francisco Gabriel. Instituto de Investigación Biosanitaria de Granada; España
Fil: Gómez, Carlos Germán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina
Fil: González Martinez, Coral. Universidad de Granada; España
Fil: Valero, Teresa. Universidad de Granada; España
Fil: Expósito Hernández, José. Universidad de Granada; España
Fil: Pucheta, Pablo Ignacio. Universidad de Granada; España
Fil: Rodriguez Martinez, Alba. Universidad de Granada; España
Fil: Serrano, María José. Universidad de Granada; España
Fil: Lorente, José Antonio. Universidad de Granada; España
Fil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Materia
CANCER BIOMARKER
CANCER DIAGNOSIS
LIQUID BIOPSY
MAGNETIC MICROBEADS
MICROFLUIDIC IMMUNOSENSOR
PSA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/201755
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/201755
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A Novel, Quick, and Reliable Smartphone-Based Method for Serum PSA Quantification: Original Design of a Portable Microfluidic Immunosensor-Based SystemOrtega Sánchez, Francisco GabrielGómez, Carlos GermánGonzález Martinez, CoralValero, TeresaExpósito Hernández, JoséPucheta, Pablo IgnacioRodriguez Martinez, AlbaSerrano, María JoséLorente, José AntonioFernández Baldo, Martín AlejandroCANCER BIOMARKERCANCER DIAGNOSISLIQUID BIOPSYMAGNETIC MICROBEADSMICROFLUIDIC IMMUNOSENSORPSAhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1We describe a versatile, portable, and simple platform that includes a microfluidic electrochemical immunosensor for prostate-specific antigen (PSA) detection. It is based on the covalent immobilization of the anti-PSA monoclonal antibody on magnetic microbeads retained in the central channel of a microfluidic device. Image flow cytometry and scanning electron microscopy were used to characterize the magnetic microbeads. A direct sandwich immunoassay (with horseradish peroxidase-conjugated PSA antibody) served to quantify the cancer biomarker in serum samples. The enzymatic product was detected at −100 mV by amperometry on sputtered thin-film electrodes. Electrochemical reaction produced a current proportional to the PSA level, with a linear range from 10 pg mL−1 to 1500 pg mL−1. The sensitivity was demonstrated by a detection limit of 2 pg mL−1 and the reproducibility by a coefficient of variation of 6.16%. The clinical performance of this platform was tested in serum samples from patients with prostate cancer (PCa), observing high specificity and full correlation with gold standard determinations. In conclusion, this analytical platform is a promising tool for measuring PSA levels in patients with PCa, offering a high sensitivity and reduced variability. The small platform size and low cost of this quantitative methodology support its suitability for the fast and sensitive analysis of PSA and other circulating biomarkers in patients. Further research is warranted to verify these findings and explore its potential application at all healthcare levels.Fil: Ortega Sánchez, Francisco Gabriel. Instituto de Investigación Biosanitaria de Granada; EspañaFil: Gómez, Carlos Germán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; ArgentinaFil: González Martinez, Coral. Universidad de Granada; EspañaFil: Valero, Teresa. Universidad de Granada; EspañaFil: Expósito Hernández, José. Universidad de Granada; EspañaFil: Pucheta, Pablo Ignacio. Universidad de Granada; EspañaFil: Rodriguez Martinez, Alba. Universidad de Granada; EspañaFil: Serrano, María José. Universidad de Granada; EspañaFil: Lorente, José Antonio. Universidad de Granada; EspañaFil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaMultidisciplinary Digital Publishing Institute2022-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/201755Ortega Sánchez, Francisco Gabriel; Gómez, Carlos Germán; González Martinez, Coral; Valero, Teresa; Expósito Hernández, José; et al.; A Novel, Quick, and Reliable Smartphone-Based Method for Serum PSA Quantification: Original Design of a Portable Microfluidic Immunosensor-Based System; Multidisciplinary Digital Publishing Institute; Cancers; 14; 18; 9-2022; 1-122072-6694CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2072-6694/14/18/4483info:eu-repo/semantics/altIdentifier/doi/10.3390/cancers14184483info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:19:33Zoai:ri.conicet.gov.ar:11336/201755instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:19:33.714CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A Novel, Quick, and Reliable Smartphone-Based Method for Serum PSA Quantification: Original Design of a Portable Microfluidic Immunosensor-Based System
title A Novel, Quick, and Reliable Smartphone-Based Method for Serum PSA Quantification: Original Design of a Portable Microfluidic Immunosensor-Based System
spellingShingle A Novel, Quick, and Reliable Smartphone-Based Method for Serum PSA Quantification: Original Design of a Portable Microfluidic Immunosensor-Based System
Ortega Sánchez, Francisco Gabriel
CANCER BIOMARKER
CANCER DIAGNOSIS
LIQUID BIOPSY
MAGNETIC MICROBEADS
MICROFLUIDIC IMMUNOSENSOR
PSA
title_short A Novel, Quick, and Reliable Smartphone-Based Method for Serum PSA Quantification: Original Design of a Portable Microfluidic Immunosensor-Based System
title_full A Novel, Quick, and Reliable Smartphone-Based Method for Serum PSA Quantification: Original Design of a Portable Microfluidic Immunosensor-Based System
title_fullStr A Novel, Quick, and Reliable Smartphone-Based Method for Serum PSA Quantification: Original Design of a Portable Microfluidic Immunosensor-Based System
title_full_unstemmed A Novel, Quick, and Reliable Smartphone-Based Method for Serum PSA Quantification: Original Design of a Portable Microfluidic Immunosensor-Based System
title_sort A Novel, Quick, and Reliable Smartphone-Based Method for Serum PSA Quantification: Original Design of a Portable Microfluidic Immunosensor-Based System
dc.creator.none.fl_str_mv Ortega Sánchez, Francisco Gabriel
Gómez, Carlos Germán
González Martinez, Coral
Valero, Teresa
Expósito Hernández, José
Pucheta, Pablo Ignacio
Rodriguez Martinez, Alba
Serrano, María José
Lorente, José Antonio
Fernández Baldo, Martín Alejandro
author Ortega Sánchez, Francisco Gabriel
author_facet Ortega Sánchez, Francisco Gabriel
Gómez, Carlos Germán
González Martinez, Coral
Valero, Teresa
Expósito Hernández, José
Pucheta, Pablo Ignacio
Rodriguez Martinez, Alba
Serrano, María José
Lorente, José Antonio
Fernández Baldo, Martín Alejandro
author_role author
author2 Gómez, Carlos Germán
González Martinez, Coral
Valero, Teresa
Expósito Hernández, José
Pucheta, Pablo Ignacio
Rodriguez Martinez, Alba
Serrano, María José
Lorente, José Antonio
Fernández Baldo, Martín Alejandro
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CANCER BIOMARKER
CANCER DIAGNOSIS
LIQUID BIOPSY
MAGNETIC MICROBEADS
MICROFLUIDIC IMMUNOSENSOR
PSA
topic CANCER BIOMARKER
CANCER DIAGNOSIS
LIQUID BIOPSY
MAGNETIC MICROBEADS
MICROFLUIDIC IMMUNOSENSOR
PSA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We describe a versatile, portable, and simple platform that includes a microfluidic electrochemical immunosensor for prostate-specific antigen (PSA) detection. It is based on the covalent immobilization of the anti-PSA monoclonal antibody on magnetic microbeads retained in the central channel of a microfluidic device. Image flow cytometry and scanning electron microscopy were used to characterize the magnetic microbeads. A direct sandwich immunoassay (with horseradish peroxidase-conjugated PSA antibody) served to quantify the cancer biomarker in serum samples. The enzymatic product was detected at −100 mV by amperometry on sputtered thin-film electrodes. Electrochemical reaction produced a current proportional to the PSA level, with a linear range from 10 pg mL−1 to 1500 pg mL−1. The sensitivity was demonstrated by a detection limit of 2 pg mL−1 and the reproducibility by a coefficient of variation of 6.16%. The clinical performance of this platform was tested in serum samples from patients with prostate cancer (PCa), observing high specificity and full correlation with gold standard determinations. In conclusion, this analytical platform is a promising tool for measuring PSA levels in patients with PCa, offering a high sensitivity and reduced variability. The small platform size and low cost of this quantitative methodology support its suitability for the fast and sensitive analysis of PSA and other circulating biomarkers in patients. Further research is warranted to verify these findings and explore its potential application at all healthcare levels.
Fil: Ortega Sánchez, Francisco Gabriel. Instituto de Investigación Biosanitaria de Granada; España
Fil: Gómez, Carlos Germán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina
Fil: González Martinez, Coral. Universidad de Granada; España
Fil: Valero, Teresa. Universidad de Granada; España
Fil: Expósito Hernández, José. Universidad de Granada; España
Fil: Pucheta, Pablo Ignacio. Universidad de Granada; España
Fil: Rodriguez Martinez, Alba. Universidad de Granada; España
Fil: Serrano, María José. Universidad de Granada; España
Fil: Lorente, José Antonio. Universidad de Granada; España
Fil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
description We describe a versatile, portable, and simple platform that includes a microfluidic electrochemical immunosensor for prostate-specific antigen (PSA) detection. It is based on the covalent immobilization of the anti-PSA monoclonal antibody on magnetic microbeads retained in the central channel of a microfluidic device. Image flow cytometry and scanning electron microscopy were used to characterize the magnetic microbeads. A direct sandwich immunoassay (with horseradish peroxidase-conjugated PSA antibody) served to quantify the cancer biomarker in serum samples. The enzymatic product was detected at −100 mV by amperometry on sputtered thin-film electrodes. Electrochemical reaction produced a current proportional to the PSA level, with a linear range from 10 pg mL−1 to 1500 pg mL−1. The sensitivity was demonstrated by a detection limit of 2 pg mL−1 and the reproducibility by a coefficient of variation of 6.16%. The clinical performance of this platform was tested in serum samples from patients with prostate cancer (PCa), observing high specificity and full correlation with gold standard determinations. In conclusion, this analytical platform is a promising tool for measuring PSA levels in patients with PCa, offering a high sensitivity and reduced variability. The small platform size and low cost of this quantitative methodology support its suitability for the fast and sensitive analysis of PSA and other circulating biomarkers in patients. Further research is warranted to verify these findings and explore its potential application at all healthcare levels.
publishDate 2022
dc.date.none.fl_str_mv 2022-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/201755
Ortega Sánchez, Francisco Gabriel; Gómez, Carlos Germán; González Martinez, Coral; Valero, Teresa; Expósito Hernández, José; et al.; A Novel, Quick, and Reliable Smartphone-Based Method for Serum PSA Quantification: Original Design of a Portable Microfluidic Immunosensor-Based System; Multidisciplinary Digital Publishing Institute; Cancers; 14; 18; 9-2022; 1-12
2072-6694
CONICET Digital
CONICET
url http://hdl.handle.net/11336/201755
identifier_str_mv Ortega Sánchez, Francisco Gabriel; Gómez, Carlos Germán; González Martinez, Coral; Valero, Teresa; Expósito Hernández, José; et al.; A Novel, Quick, and Reliable Smartphone-Based Method for Serum PSA Quantification: Original Design of a Portable Microfluidic Immunosensor-Based System; Multidisciplinary Digital Publishing Institute; Cancers; 14; 18; 9-2022; 1-12
2072-6694
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2072-6694/14/18/4483
info:eu-repo/semantics/altIdentifier/doi/10.3390/cancers14184483
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842981067714199552
score 12.48226

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