Determination of arylsulphatase and phosphatase enzyme activities in soil using screen-printed electrodes modified with multi-walled carbon nanotubes
- Autores
- Stege, Patricia Wanda; Messina, Germán Alejandro; Bianchi, Guillermo; Olsina, Roberto Antonio; Raba, Julio
- Año de publicación
- 2009
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Sustainability of agricultural systems has become an important issue all over the world. The activity of enzymes is potentially an important quality bioindicator in soils. The aim of the present study was to develop a simple and convenient assay to determine the activity of arylsulphatase (AS), acid (ACP) and alkaline phosphatase (ALP) in agricultural soil. The activities of these enzymes were detected using a non-electroactive substrate, which produces an electroactive product. To this end, p-aminophenyl phosphate (pAPP) was used as a substrate which is converted to p-aminophenol (pAP) after enzymatic dephosphorylation; and 4-nitrocatechol sulphate (4-NCS) was used as a substrate for AS activity based on its catalytic effect on the hydrolysis of 4-NCS into 4-nitrocatechol (4-NC). The products of both enzymatic reactions were quantified on carbon-based screen-printed electrodes (SPCEs) modified with carbon nanotubes (CNTs), using Osteryoung square-wave voltammetry (OSWV). The determination of the reaction products allowed more sensitive determination of ALP, ACP and AS activities in soil than that obtained with a spectrophotometric method. This assay also diminishes the generation of waste, which is desirable in green analytical chemistry. The optimization of the analytical procedure in terms of the nature of electrode type, applied potential, pH of solution, and precision of measurements is reported.
Fil: Stege, Patricia Wanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Messina, Germán Alejandro. Universidad Nacional de San Luis; Argentina
Fil: Bianchi, Guillermo. Universidad de Los Andes Facultad de Ciencias; Venezuela
Fil: Olsina, Roberto Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina - Materia
-
ACID PHOSPHATASE
ALKALINE PHOSPHATASE
ARYLSULPHATASE
ENZYMES ACTIVITIES
MULTI-WALLED CARBON NANOTUBES
SCREEN-PRINTED ELECTRODES
SOIL - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/92872
Ver los metadatos del registro completo
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Determination of arylsulphatase and phosphatase enzyme activities in soil using screen-printed electrodes modified with multi-walled carbon nanotubesStege, Patricia WandaMessina, Germán AlejandroBianchi, GuillermoOlsina, Roberto AntonioRaba, JulioACID PHOSPHATASEALKALINE PHOSPHATASEARYLSULPHATASEENZYMES ACTIVITIESMULTI-WALLED CARBON NANOTUBESSCREEN-PRINTED ELECTRODESSOILhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Sustainability of agricultural systems has become an important issue all over the world. The activity of enzymes is potentially an important quality bioindicator in soils. The aim of the present study was to develop a simple and convenient assay to determine the activity of arylsulphatase (AS), acid (ACP) and alkaline phosphatase (ALP) in agricultural soil. The activities of these enzymes were detected using a non-electroactive substrate, which produces an electroactive product. To this end, p-aminophenyl phosphate (pAPP) was used as a substrate which is converted to p-aminophenol (pAP) after enzymatic dephosphorylation; and 4-nitrocatechol sulphate (4-NCS) was used as a substrate for AS activity based on its catalytic effect on the hydrolysis of 4-NCS into 4-nitrocatechol (4-NC). The products of both enzymatic reactions were quantified on carbon-based screen-printed electrodes (SPCEs) modified with carbon nanotubes (CNTs), using Osteryoung square-wave voltammetry (OSWV). The determination of the reaction products allowed more sensitive determination of ALP, ACP and AS activities in soil than that obtained with a spectrophotometric method. This assay also diminishes the generation of waste, which is desirable in green analytical chemistry. The optimization of the analytical procedure in terms of the nature of electrode type, applied potential, pH of solution, and precision of measurements is reported.Fil: Stege, Patricia Wanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Messina, Germán Alejandro. Universidad Nacional de San Luis; ArgentinaFil: Bianchi, Guillermo. Universidad de Los Andes Facultad de Ciencias; VenezuelaFil: Olsina, Roberto Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaPergamon-Elsevier Science Ltd2009-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/92872Stege, Patricia Wanda; Messina, Germán Alejandro; Bianchi, Guillermo; Olsina, Roberto Antonio; Raba, Julio; Determination of arylsulphatase and phosphatase enzyme activities in soil using screen-printed electrodes modified with multi-walled carbon nanotubes; Pergamon-Elsevier Science Ltd; Soil Biology And Biochemistry; 41; 12; 12-2009; 2444-24520038-0717CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.soilbio.2009.08.024info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0038071709003265info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:18:02Zoai:ri.conicet.gov.ar:11336/92872instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:18:03.288CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Determination of arylsulphatase and phosphatase enzyme activities in soil using screen-printed electrodes modified with multi-walled carbon nanotubes |
| title |
Determination of arylsulphatase and phosphatase enzyme activities in soil using screen-printed electrodes modified with multi-walled carbon nanotubes |
| spellingShingle |
Determination of arylsulphatase and phosphatase enzyme activities in soil using screen-printed electrodes modified with multi-walled carbon nanotubes Stege, Patricia Wanda ACID PHOSPHATASE ALKALINE PHOSPHATASE ARYLSULPHATASE ENZYMES ACTIVITIES MULTI-WALLED CARBON NANOTUBES SCREEN-PRINTED ELECTRODES SOIL |
| title_short |
Determination of arylsulphatase and phosphatase enzyme activities in soil using screen-printed electrodes modified with multi-walled carbon nanotubes |
| title_full |
Determination of arylsulphatase and phosphatase enzyme activities in soil using screen-printed electrodes modified with multi-walled carbon nanotubes |
| title_fullStr |
Determination of arylsulphatase and phosphatase enzyme activities in soil using screen-printed electrodes modified with multi-walled carbon nanotubes |
| title_full_unstemmed |
Determination of arylsulphatase and phosphatase enzyme activities in soil using screen-printed electrodes modified with multi-walled carbon nanotubes |
| title_sort |
Determination of arylsulphatase and phosphatase enzyme activities in soil using screen-printed electrodes modified with multi-walled carbon nanotubes |
| dc.creator.none.fl_str_mv |
Stege, Patricia Wanda Messina, Germán Alejandro Bianchi, Guillermo Olsina, Roberto Antonio Raba, Julio |
| author |
Stege, Patricia Wanda |
| author_facet |
Stege, Patricia Wanda Messina, Germán Alejandro Bianchi, Guillermo Olsina, Roberto Antonio Raba, Julio |
| author_role |
author |
| author2 |
Messina, Germán Alejandro Bianchi, Guillermo Olsina, Roberto Antonio Raba, Julio |
| author2_role |
author author author author |
| dc.subject.none.fl_str_mv |
ACID PHOSPHATASE ALKALINE PHOSPHATASE ARYLSULPHATASE ENZYMES ACTIVITIES MULTI-WALLED CARBON NANOTUBES SCREEN-PRINTED ELECTRODES SOIL |
| topic |
ACID PHOSPHATASE ALKALINE PHOSPHATASE ARYLSULPHATASE ENZYMES ACTIVITIES MULTI-WALLED CARBON NANOTUBES SCREEN-PRINTED ELECTRODES SOIL |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
Sustainability of agricultural systems has become an important issue all over the world. The activity of enzymes is potentially an important quality bioindicator in soils. The aim of the present study was to develop a simple and convenient assay to determine the activity of arylsulphatase (AS), acid (ACP) and alkaline phosphatase (ALP) in agricultural soil. The activities of these enzymes were detected using a non-electroactive substrate, which produces an electroactive product. To this end, p-aminophenyl phosphate (pAPP) was used as a substrate which is converted to p-aminophenol (pAP) after enzymatic dephosphorylation; and 4-nitrocatechol sulphate (4-NCS) was used as a substrate for AS activity based on its catalytic effect on the hydrolysis of 4-NCS into 4-nitrocatechol (4-NC). The products of both enzymatic reactions were quantified on carbon-based screen-printed electrodes (SPCEs) modified with carbon nanotubes (CNTs), using Osteryoung square-wave voltammetry (OSWV). The determination of the reaction products allowed more sensitive determination of ALP, ACP and AS activities in soil than that obtained with a spectrophotometric method. This assay also diminishes the generation of waste, which is desirable in green analytical chemistry. The optimization of the analytical procedure in terms of the nature of electrode type, applied potential, pH of solution, and precision of measurements is reported. Fil: Stege, Patricia Wanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina Fil: Messina, Germán Alejandro. Universidad Nacional de San Luis; Argentina Fil: Bianchi, Guillermo. Universidad de Los Andes Facultad de Ciencias; Venezuela Fil: Olsina, Roberto Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina |
| description |
Sustainability of agricultural systems has become an important issue all over the world. The activity of enzymes is potentially an important quality bioindicator in soils. The aim of the present study was to develop a simple and convenient assay to determine the activity of arylsulphatase (AS), acid (ACP) and alkaline phosphatase (ALP) in agricultural soil. The activities of these enzymes were detected using a non-electroactive substrate, which produces an electroactive product. To this end, p-aminophenyl phosphate (pAPP) was used as a substrate which is converted to p-aminophenol (pAP) after enzymatic dephosphorylation; and 4-nitrocatechol sulphate (4-NCS) was used as a substrate for AS activity based on its catalytic effect on the hydrolysis of 4-NCS into 4-nitrocatechol (4-NC). The products of both enzymatic reactions were quantified on carbon-based screen-printed electrodes (SPCEs) modified with carbon nanotubes (CNTs), using Osteryoung square-wave voltammetry (OSWV). The determination of the reaction products allowed more sensitive determination of ALP, ACP and AS activities in soil than that obtained with a spectrophotometric method. This assay also diminishes the generation of waste, which is desirable in green analytical chemistry. The optimization of the analytical procedure in terms of the nature of electrode type, applied potential, pH of solution, and precision of measurements is reported. |
| publishDate |
2009 |
| dc.date.none.fl_str_mv |
2009-12 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/92872 Stege, Patricia Wanda; Messina, Germán Alejandro; Bianchi, Guillermo; Olsina, Roberto Antonio; Raba, Julio; Determination of arylsulphatase and phosphatase enzyme activities in soil using screen-printed electrodes modified with multi-walled carbon nanotubes; Pergamon-Elsevier Science Ltd; Soil Biology And Biochemistry; 41; 12; 12-2009; 2444-2452 0038-0717 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/92872 |
| identifier_str_mv |
Stege, Patricia Wanda; Messina, Germán Alejandro; Bianchi, Guillermo; Olsina, Roberto Antonio; Raba, Julio; Determination of arylsulphatase and phosphatase enzyme activities in soil using screen-printed electrodes modified with multi-walled carbon nanotubes; Pergamon-Elsevier Science Ltd; Soil Biology And Biochemistry; 41; 12; 12-2009; 2444-2452 0038-0717 CONICET Digital CONICET |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
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info:eu-repo/semantics/altIdentifier/doi/10.1016/j.soilbio.2009.08.024 info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0038071709003265 |
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openAccess |
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application/pdf application/pdf application/pdf application/pdf |
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Pergamon-Elsevier Science Ltd |
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Pergamon-Elsevier Science Ltd |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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