N-acetylcysteine reduces markers of differentiation in 3T3-L1 adipocytes

Autores
Calzadilla, Pablo Ignacio; Sapochnik, Daiana; Cosentino, María Soledad; Diz, Virginia Emilse; Dicelio, Lelia Elina; Calvo, Juan Carlos; Guerra, Liliana Noemi
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Oxidative stress plays a critical role in the pathogenesis of diabetes, hypertension and atherosclerosis. Some authors reported that fat accumulation correlates to systemic oxidative stress in humans and mice, but the relationship of lipid production and oxidative metabolism is still unclear. In our laboratory we used 3T3-L1 preadipocytes, which are able to differentiate into mature adipocytes and accumulate lipids, as obesity model. We showed that intracellular reactive oxygen species (ROS) and antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities increased in parallel with fat accumulation. Meanwhile N-acetylcysteine (NAC), a well known antioxidant and Glutathione (GSH) precursor, inhibited ROS levels as well as fat accumulation in a concentration-dependent manner. NAC also inhibited both adipogenic transcription factors CCAAT/enhancer binding protein beta (C/EBP) and peroxisomal proliferator activated receptor gamma (PPAR β) expression; we suggested that intracellular GSH content could be responsible for these effects.
Fil: Calzadilla, Pablo Ignacio. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Quimica Biologica. Laboratorio de Química de Proteoglicanos y Matriz Extracelular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sapochnik, Daiana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cosentino, María Soledad. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Quimica Biologica. Laboratorio de Química de Proteoglicanos y Matriz Extracelular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Diz, Virginia Emilse. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica, Analítica y Quimica Fisica; Argentina
Fil: Dicelio, Lelia Elina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica, Analítica y Quimica Fisica; Argentina
Fil: Calvo, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Quimica Biologica. Laboratorio de Química de Proteoglicanos y Matriz Extracelular; Argentina
Fil: Guerra, Liliana Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Quimica Biologica. Laboratorio de Química de Proteoglicanos y Matriz Extracelular; Argentina
Materia
ADIPOCYTE DIFFERENTIATION
NAC
TRIGLYCERIDE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/14010
Acceder
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network_name_str CONICET Digital (CONICET)
spelling N-acetylcysteine reduces markers of differentiation in 3T3-L1 adipocytesCalzadilla, Pablo IgnacioSapochnik, DaianaCosentino, María SoledadDiz, Virginia EmilseDicelio, Lelia ElinaCalvo, Juan CarlosGuerra, Liliana NoemiADIPOCYTE DIFFERENTIATIONNACTRIGLYCERIDEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Oxidative stress plays a critical role in the pathogenesis of diabetes, hypertension and atherosclerosis. Some authors reported that fat accumulation correlates to systemic oxidative stress in humans and mice, but the relationship of lipid production and oxidative metabolism is still unclear. In our laboratory we used 3T3-L1 preadipocytes, which are able to differentiate into mature adipocytes and accumulate lipids, as obesity model. We showed that intracellular reactive oxygen species (ROS) and antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities increased in parallel with fat accumulation. Meanwhile N-acetylcysteine (NAC), a well known antioxidant and Glutathione (GSH) precursor, inhibited ROS levels as well as fat accumulation in a concentration-dependent manner. NAC also inhibited both adipogenic transcription factors CCAAT/enhancer binding protein beta (C/EBP) and peroxisomal proliferator activated receptor gamma (PPAR β) expression; we suggested that intracellular GSH content could be responsible for these effects.Fil: Calzadilla, Pablo Ignacio. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Quimica Biologica. Laboratorio de Química de Proteoglicanos y Matriz Extracelular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sapochnik, Daiana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cosentino, María Soledad. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Quimica Biologica. Laboratorio de Química de Proteoglicanos y Matriz Extracelular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Diz, Virginia Emilse. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica, Analítica y Quimica Fisica; ArgentinaFil: Dicelio, Lelia Elina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica, Analítica y Quimica Fisica; ArgentinaFil: Calvo, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Quimica Biologica. Laboratorio de Química de Proteoglicanos y Matriz Extracelular; ArgentinaFil: Guerra, Liliana Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Quimica Biologica. Laboratorio de Química de Proteoglicanos y Matriz Extracelular; ArgentinaMolecular Diversity Preservation International2011-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/14010Calzadilla, Pablo Ignacio; Sapochnik, Daiana; Cosentino, María Soledad; Diz, Virginia Emilse; Dicelio, Lelia Elina; et al.; N-acetylcysteine reduces markers of differentiation in 3T3-L1 adipocytes; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 12; 10; 10-2011; 6936-69511422-00671422-0067enginfo:eu-repo/semantics/altIdentifier/url/http://www.mdpi.com/1422-0067/12/10/6936info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms12106936info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3211019/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:11:26Zoai:ri.conicet.gov.ar:11336/14010instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:11:26.867CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv N-acetylcysteine reduces markers of differentiation in 3T3-L1 adipocytes
title N-acetylcysteine reduces markers of differentiation in 3T3-L1 adipocytes
spellingShingle N-acetylcysteine reduces markers of differentiation in 3T3-L1 adipocytes
Calzadilla, Pablo Ignacio
ADIPOCYTE DIFFERENTIATION
NAC
TRIGLYCERIDE
title_short N-acetylcysteine reduces markers of differentiation in 3T3-L1 adipocytes
title_full N-acetylcysteine reduces markers of differentiation in 3T3-L1 adipocytes
title_fullStr N-acetylcysteine reduces markers of differentiation in 3T3-L1 adipocytes
title_full_unstemmed N-acetylcysteine reduces markers of differentiation in 3T3-L1 adipocytes
title_sort N-acetylcysteine reduces markers of differentiation in 3T3-L1 adipocytes
dc.creator.none.fl_str_mv Calzadilla, Pablo Ignacio
Sapochnik, Daiana
Cosentino, María Soledad
Diz, Virginia Emilse
Dicelio, Lelia Elina
Calvo, Juan Carlos
Guerra, Liliana Noemi
author Calzadilla, Pablo Ignacio
author_facet Calzadilla, Pablo Ignacio
Sapochnik, Daiana
Cosentino, María Soledad
Diz, Virginia Emilse
Dicelio, Lelia Elina
Calvo, Juan Carlos
Guerra, Liliana Noemi
author_role author
author2 Sapochnik, Daiana
Cosentino, María Soledad
Diz, Virginia Emilse
Dicelio, Lelia Elina
Calvo, Juan Carlos
Guerra, Liliana Noemi
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ADIPOCYTE DIFFERENTIATION
NAC
TRIGLYCERIDE
topic ADIPOCYTE DIFFERENTIATION
NAC
TRIGLYCERIDE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Oxidative stress plays a critical role in the pathogenesis of diabetes, hypertension and atherosclerosis. Some authors reported that fat accumulation correlates to systemic oxidative stress in humans and mice, but the relationship of lipid production and oxidative metabolism is still unclear. In our laboratory we used 3T3-L1 preadipocytes, which are able to differentiate into mature adipocytes and accumulate lipids, as obesity model. We showed that intracellular reactive oxygen species (ROS) and antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities increased in parallel with fat accumulation. Meanwhile N-acetylcysteine (NAC), a well known antioxidant and Glutathione (GSH) precursor, inhibited ROS levels as well as fat accumulation in a concentration-dependent manner. NAC also inhibited both adipogenic transcription factors CCAAT/enhancer binding protein beta (C/EBP) and peroxisomal proliferator activated receptor gamma (PPAR β) expression; we suggested that intracellular GSH content could be responsible for these effects.
Fil: Calzadilla, Pablo Ignacio. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Quimica Biologica. Laboratorio de Química de Proteoglicanos y Matriz Extracelular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sapochnik, Daiana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cosentino, María Soledad. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Quimica Biologica. Laboratorio de Química de Proteoglicanos y Matriz Extracelular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Diz, Virginia Emilse. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica, Analítica y Quimica Fisica; Argentina
Fil: Dicelio, Lelia Elina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica, Analítica y Quimica Fisica; Argentina
Fil: Calvo, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Quimica Biologica. Laboratorio de Química de Proteoglicanos y Matriz Extracelular; Argentina
Fil: Guerra, Liliana Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Quimica Biologica. Laboratorio de Química de Proteoglicanos y Matriz Extracelular; Argentina
description Oxidative stress plays a critical role in the pathogenesis of diabetes, hypertension and atherosclerosis. Some authors reported that fat accumulation correlates to systemic oxidative stress in humans and mice, but the relationship of lipid production and oxidative metabolism is still unclear. In our laboratory we used 3T3-L1 preadipocytes, which are able to differentiate into mature adipocytes and accumulate lipids, as obesity model. We showed that intracellular reactive oxygen species (ROS) and antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities increased in parallel with fat accumulation. Meanwhile N-acetylcysteine (NAC), a well known antioxidant and Glutathione (GSH) precursor, inhibited ROS levels as well as fat accumulation in a concentration-dependent manner. NAC also inhibited both adipogenic transcription factors CCAAT/enhancer binding protein beta (C/EBP) and peroxisomal proliferator activated receptor gamma (PPAR β) expression; we suggested that intracellular GSH content could be responsible for these effects.
publishDate 2011
dc.date.none.fl_str_mv 2011-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/14010
Calzadilla, Pablo Ignacio; Sapochnik, Daiana; Cosentino, María Soledad; Diz, Virginia Emilse; Dicelio, Lelia Elina; et al.; N-acetylcysteine reduces markers of differentiation in 3T3-L1 adipocytes; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 12; 10; 10-2011; 6936-6951
1422-0067
1422-0067
url http://hdl.handle.net/11336/14010
identifier_str_mv Calzadilla, Pablo Ignacio; Sapochnik, Daiana; Cosentino, María Soledad; Diz, Virginia Emilse; Dicelio, Lelia Elina; et al.; N-acetylcysteine reduces markers of differentiation in 3T3-L1 adipocytes; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 12; 10; 10-2011; 6936-6951
1422-0067
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.mdpi.com/1422-0067/12/10/6936
info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms12106936
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3211019/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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