N-Acetylcysteine affects obesity-related protein expression in 3T3-L1 adipocytes

Autores
Calzadilla, Pablo Ignacio; Gómez Serrano, M.; Garcìa Santos, E.; Schiappacasse, Agustina; Abalde, Yasmin; Calvo, Juan Carlos; Peral, B.; Guerra, Liliana Noemi
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objectives: Oxidative stress plays critical roles in the pathogeneses of diabetes, hypertension, and atherosclerosis, but its effect on fat accumulation is still unclear. In this study, we analyzed the role of the well-known antioxidant and a glutathione (GSH) precursor N-acetylcysteine (NAC) in fat accumulation and the expression of obesity-associated proteins. Methods: We studied the effects of 10 μM NAC on obesity-related protein expression in cultured 3T3-L1 preadipocytes, which are able to differentiate into mature adipocytes and accumulate lipids. Results: NAC treatment inhibited fat accumulation and reduced the expression of obesity-related proteins, including monoamine oxidase A, heat shock protein 70 (HSP70), aminoacylase -1 (ACY-1), and transketolase. Discussion: Our results suggest that the effects of NAC on triglycerides (Tgs) and protein expression are correlated. In support of this, we showed that NAC treatment affected both the Tg synthesis pathway and the expression levels of proteins implicated in human obesity.
Fil: Calzadilla, Pablo Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Gómez Serrano, M.. Consejo Superior de Investigaciones Científicas; España. Universidad Autónoma de Madrid; España
Fil: Garcìa Santos, E.. Consejo Superior de Investigaciones Científica; España. Universidad Autónoma de Madrid; España
Fil: Schiappacasse, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Abalde, Yasmin. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Calvo, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Peral, B.. Consejo Superior de Investigaciones Científicas; España. Universidad Autónoma de Madrid; España
Fil: Guerra, Liliana Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Materia
Adipocytes
3t3-L1
Redox
Differentiation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/5733
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/5733
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling N-Acetylcysteine affects obesity-related protein expression in 3T3-L1 adipocytesCalzadilla, Pablo IgnacioGómez Serrano, M.Garcìa Santos, E.Schiappacasse, AgustinaAbalde, YasminCalvo, Juan CarlosPeral, B.Guerra, Liliana NoemiAdipocytes3t3-L1RedoxDifferentiationhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Objectives: Oxidative stress plays critical roles in the pathogeneses of diabetes, hypertension, and atherosclerosis, but its effect on fat accumulation is still unclear. In this study, we analyzed the role of the well-known antioxidant and a glutathione (GSH) precursor N-acetylcysteine (NAC) in fat accumulation and the expression of obesity-associated proteins. Methods: We studied the effects of 10 μM NAC on obesity-related protein expression in cultured 3T3-L1 preadipocytes, which are able to differentiate into mature adipocytes and accumulate lipids. Results: NAC treatment inhibited fat accumulation and reduced the expression of obesity-related proteins, including monoamine oxidase A, heat shock protein 70 (HSP70), aminoacylase -1 (ACY-1), and transketolase. Discussion: Our results suggest that the effects of NAC on triglycerides (Tgs) and protein expression are correlated. In support of this, we showed that NAC treatment affected both the Tg synthesis pathway and the expression levels of proteins implicated in human obesity.Fil: Calzadilla, Pablo Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Gómez Serrano, M.. Consejo Superior de Investigaciones Científicas; España. Universidad Autónoma de Madrid; EspañaFil: Garcìa Santos, E.. Consejo Superior de Investigaciones Científica; España. Universidad Autónoma de Madrid; EspañaFil: Schiappacasse, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Abalde, Yasmin. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Calvo, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Peral, B.. Consejo Superior de Investigaciones Científicas; España. Universidad Autónoma de Madrid; EspañaFil: Guerra, Liliana Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaManey Publishing2013-11-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/mswordapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/5733Calzadilla, Pablo Ignacio; Gómez Serrano, M.; Garcìa Santos, E.; Schiappacasse, Agustina; Abalde, Yasmin; et al.; N-Acetylcysteine affects obesity-related protein expression in 3T3-L1 adipocytes; Maney Publishing; Redox Report; 18; 6; 15-11-2013; 210-2181351-00021743-2928enginfo:eu-repo/semantics/altIdentifier/doi/10.1179/1351000213Y.0000000066info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/pmid/24112955info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.1179/1351000213Y.0000000066info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:09:43Zoai:ri.conicet.gov.ar:11336/5733instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:09:43.329CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv N-Acetylcysteine affects obesity-related protein expression in 3T3-L1 adipocytes
title N-Acetylcysteine affects obesity-related protein expression in 3T3-L1 adipocytes
spellingShingle N-Acetylcysteine affects obesity-related protein expression in 3T3-L1 adipocytes
Calzadilla, Pablo Ignacio
Adipocytes
3t3-L1
Redox
Differentiation
title_short N-Acetylcysteine affects obesity-related protein expression in 3T3-L1 adipocytes
title_full N-Acetylcysteine affects obesity-related protein expression in 3T3-L1 adipocytes
title_fullStr N-Acetylcysteine affects obesity-related protein expression in 3T3-L1 adipocytes
title_full_unstemmed N-Acetylcysteine affects obesity-related protein expression in 3T3-L1 adipocytes
title_sort N-Acetylcysteine affects obesity-related protein expression in 3T3-L1 adipocytes
dc.creator.none.fl_str_mv Calzadilla, Pablo Ignacio
Gómez Serrano, M.
Garcìa Santos, E.
Schiappacasse, Agustina
Abalde, Yasmin
Calvo, Juan Carlos
Peral, B.
Guerra, Liliana Noemi
author Calzadilla, Pablo Ignacio
author_facet Calzadilla, Pablo Ignacio
Gómez Serrano, M.
Garcìa Santos, E.
Schiappacasse, Agustina
Abalde, Yasmin
Calvo, Juan Carlos
Peral, B.
Guerra, Liliana Noemi
author_role author
author2 Gómez Serrano, M.
Garcìa Santos, E.
Schiappacasse, Agustina
Abalde, Yasmin
Calvo, Juan Carlos
Peral, B.
Guerra, Liliana Noemi
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Adipocytes
3t3-L1
Redox
Differentiation
topic Adipocytes
3t3-L1
Redox
Differentiation
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Objectives: Oxidative stress plays critical roles in the pathogeneses of diabetes, hypertension, and atherosclerosis, but its effect on fat accumulation is still unclear. In this study, we analyzed the role of the well-known antioxidant and a glutathione (GSH) precursor N-acetylcysteine (NAC) in fat accumulation and the expression of obesity-associated proteins. Methods: We studied the effects of 10 μM NAC on obesity-related protein expression in cultured 3T3-L1 preadipocytes, which are able to differentiate into mature adipocytes and accumulate lipids. Results: NAC treatment inhibited fat accumulation and reduced the expression of obesity-related proteins, including monoamine oxidase A, heat shock protein 70 (HSP70), aminoacylase -1 (ACY-1), and transketolase. Discussion: Our results suggest that the effects of NAC on triglycerides (Tgs) and protein expression are correlated. In support of this, we showed that NAC treatment affected both the Tg synthesis pathway and the expression levels of proteins implicated in human obesity.
Fil: Calzadilla, Pablo Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Gómez Serrano, M.. Consejo Superior de Investigaciones Científicas; España. Universidad Autónoma de Madrid; España
Fil: Garcìa Santos, E.. Consejo Superior de Investigaciones Científica; España. Universidad Autónoma de Madrid; España
Fil: Schiappacasse, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Abalde, Yasmin. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Calvo, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Peral, B.. Consejo Superior de Investigaciones Científicas; España. Universidad Autónoma de Madrid; España
Fil: Guerra, Liliana Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
description Objectives: Oxidative stress plays critical roles in the pathogeneses of diabetes, hypertension, and atherosclerosis, but its effect on fat accumulation is still unclear. In this study, we analyzed the role of the well-known antioxidant and a glutathione (GSH) precursor N-acetylcysteine (NAC) in fat accumulation and the expression of obesity-associated proteins. Methods: We studied the effects of 10 μM NAC on obesity-related protein expression in cultured 3T3-L1 preadipocytes, which are able to differentiate into mature adipocytes and accumulate lipids. Results: NAC treatment inhibited fat accumulation and reduced the expression of obesity-related proteins, including monoamine oxidase A, heat shock protein 70 (HSP70), aminoacylase -1 (ACY-1), and transketolase. Discussion: Our results suggest that the effects of NAC on triglycerides (Tgs) and protein expression are correlated. In support of this, we showed that NAC treatment affected both the Tg synthesis pathway and the expression levels of proteins implicated in human obesity.
publishDate 2013
dc.date.none.fl_str_mv 2013-11-15
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/5733
Calzadilla, Pablo Ignacio; Gómez Serrano, M.; Garcìa Santos, E.; Schiappacasse, Agustina; Abalde, Yasmin; et al.; N-Acetylcysteine affects obesity-related protein expression in 3T3-L1 adipocytes; Maney Publishing; Redox Report; 18; 6; 15-11-2013; 210-218
1351-0002
1743-2928
url http://hdl.handle.net/11336/5733
identifier_str_mv Calzadilla, Pablo Ignacio; Gómez Serrano, M.; Garcìa Santos, E.; Schiappacasse, Agustina; Abalde, Yasmin; et al.; N-Acetylcysteine affects obesity-related protein expression in 3T3-L1 adipocytes; Maney Publishing; Redox Report; 18; 6; 15-11-2013; 210-218
1351-0002
1743-2928
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1179/1351000213Y.0000000066
info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/pmid/24112955
info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.1179/1351000213Y.0000000066
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/msword
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Maney Publishing
publisher.none.fl_str_mv Maney Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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