N-acetylcysteine reduces markers of differentiation in 3T3-L1 adipocytes

• Autores: Calzadilla, P.; Sapochnik, D.; Cosentino, S.; Diz, V.; Dicelio, L.; Calvo, J.C.; Guerra, L.N.
• Año de publicación: 2011
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Int. J. Mol. Sci. 2011;12(10):6936-6951
• Materia: Adipocyte differentiation; NAC; Triglyceride; acetylcysteine; beta actin; CCAAT enhancer binding protein; copper zinc superoxide dismutase; glutathione peroxidase; glutathione peroxidase 1; insulin receptor; manganese superoxide dismutase; metallothionein; peroxisome proliferator activated receptor gamma; protein kinase B; reactive oxygen metabolite; somatomedin C; superoxide dismutase; triacylglycerol; acetylcysteine; biological marker; CCAAT enhancer binding protein beta; glutathione; glutathione peroxidase; peroxisome proliferator activated receptor gamma; reactive oxygen metabolite; superoxide dismutase; triacylglycerol; adipocyte; aerobic metabolism; article; cell culture; cell differentiation; concentration response; controlled study; culture medium; DNA content; down regulation; enzyme activity; enzyme assay; human; human cell; lipid peroxidation; lipid storage; low drug dose; obesity; oxidative stress; protein content; protein expression; protein phosphorylation; Western blotting; 3T3 cell line; adipocyte; animal; cell differentiation; cytology; metabolism; mouse; Mus; 3T3-L1 Cells; Acetylcysteine; Adipocytes; Animals; Biological Markers; CCAAT-Enhancer-Binding Protein-beta; Cell Differentiation; Glutathione; Glutathione Peroxidase; Mice; PPAR gamma; Reactive Oxygen Species; Superoxide Dismutase; Triglycerides
• Institución: Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales

Oxidative stress plays a critical role in the pathogenesis of diabetes, hypertension and atherosclerosis. Some authors reported that fat accumulation correlates to systemic oxidative stress in humans and mice, but the relationship of lipid production and oxidative metabolism is still unclear. In our laboratory we used 3T3-L1 preadipocytes, which are able to differentiate into mature adipocytes and accumulate lipids, as obesity model. We showed that intracellular reactive oxygen species (ROS) and antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities increased in parallel with fat accumulation. Meanwhile N-acetylcysteine (NAC), a well known antioxidant and Glutathione (GSH) precursor, inhibited ROS levels as well as fat accumulation in a concentration-dependent manner. NAC also inhibited both adipogenic transcription factors CCAAT/enhancer binding protein beta (C/EBP) and peroxisomal proliferator activated receptor gamma (PPAR β) expression; we suggested that intracellular GSH content could be responsible for these effects. © 2011 by the authors; licensee MDPI, Basel, Switzerland.
Fil:Calzadilla, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Sapochnik, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Cosentino, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Diz, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Dicelio, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Calvo, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Guerra, L.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.