Transgenesis in farm animals

Autores
Salamone, Daniel Felipe; Bevacqua, Romina Jimena; Hiriart, María Inés; Buemo, Carla Paola; Luchetti, Carolina Griselda; Moro, Lucía Natalia; Fernández y Martín, Rafael
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The production of transgenic animals has many biotechnological applications; however, available techniques still remain inefficient. This review summarizes the contributions of our group to transgenesis in domestic animal. One of the most traditional transgenesis techniques in farm animals is somatic cell nuclear transfer using genetically modified somatic cells. Using this technique, we produced transgenic cloned cows capable of producing human growth hormone in the milk, and viable offspring was obtained after recloning of these transgenic animals. Another technique available to produce transgenic animals is intracytoplasmic sperm injection (ICSI). With this technique, we could obtain transgenic embryos in five different domestic species (bovine, ovine, feline, porcine and equine). To improve transgenic ICSI (ICSI-Tg) efficiency in cattle, different chemical activation treatments were evaluated, including ionomycin followed by 6-Dimethylaminopurine (6-DMAP), strontium chloride and ethanol. In addition, in the latest years, our group has developed several alternative methods to produce transgenic animals involving injection of ooplasmic vesicles, liposomes or cells previously incubated with the transgene, into zygotes or enucleated oocytes respectively. All of these treatments proved to be efficient to induce the expression of exogenous DNA into pre-implantatory embryos. However, high levels of transgene expression mosaicism were detected. For that reason, different approaches were also tested to reverse mosaicism, including a novel cloning gamete technique. We conclude this review with a brief description of novel molecular tools for transgenesis, including enzymes such as transposases, Zinc finger and Talem nucleases, which could help to increase transgenesis efficiency in domestic species. The new transgenesis variants described in this review aim to turn animal transgenesis accessible to a greater number of research groups.
Fil: Salamone, Daniel Felipe. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal. Cátedra de Fisiología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bevacqua, Romina Jimena. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal. Cátedra de Fisiología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hiriart, María Inés. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal. Cátedra de Fisiología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Buemo, Carla Paola. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal. Cátedra de Fisiología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Luchetti, Carolina Griselda. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal. Cátedra de Fisiología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Moro, Lucía Natalia. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal. Cátedra de Fisiología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernández y Martín, Rafael. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal. Cátedra de Fisiología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
GENE TRANSFER
ICSI-TG
MICROMANIPULATION
TRANSGENESIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/272076
Acceder
id CONICETDig_04d44c004b86c46b9f253511e4b752ca
oai_identifier_str oai:ri.conicet.gov.ar:11336/272076
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Transgenesis in farm animalsSalamone, Daniel FelipeBevacqua, Romina JimenaHiriart, María InésBuemo, Carla PaolaLuchetti, Carolina GriseldaMoro, Lucía NataliaFernández y Martín, RafaelGENE TRANSFERICSI-TGMICROMANIPULATIONTRANSGENESIShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The production of transgenic animals has many biotechnological applications; however, available techniques still remain inefficient. This review summarizes the contributions of our group to transgenesis in domestic animal. One of the most traditional transgenesis techniques in farm animals is somatic cell nuclear transfer using genetically modified somatic cells. Using this technique, we produced transgenic cloned cows capable of producing human growth hormone in the milk, and viable offspring was obtained after recloning of these transgenic animals. Another technique available to produce transgenic animals is intracytoplasmic sperm injection (ICSI). With this technique, we could obtain transgenic embryos in five different domestic species (bovine, ovine, feline, porcine and equine). To improve transgenic ICSI (ICSI-Tg) efficiency in cattle, different chemical activation treatments were evaluated, including ionomycin followed by 6-Dimethylaminopurine (6-DMAP), strontium chloride and ethanol. In addition, in the latest years, our group has developed several alternative methods to produce transgenic animals involving injection of ooplasmic vesicles, liposomes or cells previously incubated with the transgene, into zygotes or enucleated oocytes respectively. All of these treatments proved to be efficient to induce the expression of exogenous DNA into pre-implantatory embryos. However, high levels of transgene expression mosaicism were detected. For that reason, different approaches were also tested to reverse mosaicism, including a novel cloning gamete technique. We conclude this review with a brief description of novel molecular tools for transgenesis, including enzymes such as transposases, Zinc finger and Talem nucleases, which could help to increase transgenesis efficiency in domestic species. The new transgenesis variants described in this review aim to turn animal transgenesis accessible to a greater number of research groups.Fil: Salamone, Daniel Felipe. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal. Cátedra de Fisiología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bevacqua, Romina Jimena. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal. Cátedra de Fisiología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Hiriart, María Inés. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal. Cátedra de Fisiología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Buemo, Carla Paola. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal. Cátedra de Fisiología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Luchetti, Carolina Griselda. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal. Cátedra de Fisiología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Moro, Lucía Natalia. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal. Cátedra de Fisiología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fernández y Martín, Rafael. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal. Cátedra de Fisiología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaBrazilian College of Animal Reproduction2012-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/272076Salamone, Daniel Felipe; Bevacqua, Romina Jimena; Hiriart, María Inés; Buemo, Carla Paola; Luchetti, Carolina Griselda; et al.; Transgenesis in farm animals; Brazilian College of Animal Reproduction; Animal Reproduction; 12-2012; 772-7761806-9614CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.cbra.org.br/pages/publicacoes/animalreproduction/issues/download/v9n4/p772-776.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:38:23Zoai:ri.conicet.gov.ar:11336/272076instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:38:23.536CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Transgenesis in farm animals
title Transgenesis in farm animals
spellingShingle Transgenesis in farm animals
Salamone, Daniel Felipe
GENE TRANSFER
ICSI-TG
MICROMANIPULATION
TRANSGENESIS
title_short Transgenesis in farm animals
title_full Transgenesis in farm animals
title_fullStr Transgenesis in farm animals
title_full_unstemmed Transgenesis in farm animals
title_sort Transgenesis in farm animals
dc.creator.none.fl_str_mv Salamone, Daniel Felipe
Bevacqua, Romina Jimena
Hiriart, María Inés
Buemo, Carla Paola
Luchetti, Carolina Griselda
Moro, Lucía Natalia
Fernández y Martín, Rafael
author Salamone, Daniel Felipe
author_facet Salamone, Daniel Felipe
Bevacqua, Romina Jimena
Hiriart, María Inés
Buemo, Carla Paola
Luchetti, Carolina Griselda
Moro, Lucía Natalia
Fernández y Martín, Rafael
author_role author
author2 Bevacqua, Romina Jimena
Hiriart, María Inés
Buemo, Carla Paola
Luchetti, Carolina Griselda
Moro, Lucía Natalia
Fernández y Martín, Rafael
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv GENE TRANSFER
ICSI-TG
MICROMANIPULATION
TRANSGENESIS
topic GENE TRANSFER
ICSI-TG
MICROMANIPULATION
TRANSGENESIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The production of transgenic animals has many biotechnological applications; however, available techniques still remain inefficient. This review summarizes the contributions of our group to transgenesis in domestic animal. One of the most traditional transgenesis techniques in farm animals is somatic cell nuclear transfer using genetically modified somatic cells. Using this technique, we produced transgenic cloned cows capable of producing human growth hormone in the milk, and viable offspring was obtained after recloning of these transgenic animals. Another technique available to produce transgenic animals is intracytoplasmic sperm injection (ICSI). With this technique, we could obtain transgenic embryos in five different domestic species (bovine, ovine, feline, porcine and equine). To improve transgenic ICSI (ICSI-Tg) efficiency in cattle, different chemical activation treatments were evaluated, including ionomycin followed by 6-Dimethylaminopurine (6-DMAP), strontium chloride and ethanol. In addition, in the latest years, our group has developed several alternative methods to produce transgenic animals involving injection of ooplasmic vesicles, liposomes or cells previously incubated with the transgene, into zygotes or enucleated oocytes respectively. All of these treatments proved to be efficient to induce the expression of exogenous DNA into pre-implantatory embryos. However, high levels of transgene expression mosaicism were detected. For that reason, different approaches were also tested to reverse mosaicism, including a novel cloning gamete technique. We conclude this review with a brief description of novel molecular tools for transgenesis, including enzymes such as transposases, Zinc finger and Talem nucleases, which could help to increase transgenesis efficiency in domestic species. The new transgenesis variants described in this review aim to turn animal transgenesis accessible to a greater number of research groups.
Fil: Salamone, Daniel Felipe. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal. Cátedra de Fisiología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bevacqua, Romina Jimena. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal. Cátedra de Fisiología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hiriart, María Inés. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal. Cátedra de Fisiología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Buemo, Carla Paola. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal. Cátedra de Fisiología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Luchetti, Carolina Griselda. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal. Cátedra de Fisiología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Moro, Lucía Natalia. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal. Cátedra de Fisiología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernández y Martín, Rafael. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal. Cátedra de Fisiología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The production of transgenic animals has many biotechnological applications; however, available techniques still remain inefficient. This review summarizes the contributions of our group to transgenesis in domestic animal. One of the most traditional transgenesis techniques in farm animals is somatic cell nuclear transfer using genetically modified somatic cells. Using this technique, we produced transgenic cloned cows capable of producing human growth hormone in the milk, and viable offspring was obtained after recloning of these transgenic animals. Another technique available to produce transgenic animals is intracytoplasmic sperm injection (ICSI). With this technique, we could obtain transgenic embryos in five different domestic species (bovine, ovine, feline, porcine and equine). To improve transgenic ICSI (ICSI-Tg) efficiency in cattle, different chemical activation treatments were evaluated, including ionomycin followed by 6-Dimethylaminopurine (6-DMAP), strontium chloride and ethanol. In addition, in the latest years, our group has developed several alternative methods to produce transgenic animals involving injection of ooplasmic vesicles, liposomes or cells previously incubated with the transgene, into zygotes or enucleated oocytes respectively. All of these treatments proved to be efficient to induce the expression of exogenous DNA into pre-implantatory embryos. However, high levels of transgene expression mosaicism were detected. For that reason, different approaches were also tested to reverse mosaicism, including a novel cloning gamete technique. We conclude this review with a brief description of novel molecular tools for transgenesis, including enzymes such as transposases, Zinc finger and Talem nucleases, which could help to increase transgenesis efficiency in domestic species. The new transgenesis variants described in this review aim to turn animal transgenesis accessible to a greater number of research groups.
publishDate 2012
dc.date.none.fl_str_mv 2012-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/272076
Salamone, Daniel Felipe; Bevacqua, Romina Jimena; Hiriart, María Inés; Buemo, Carla Paola; Luchetti, Carolina Griselda; et al.; Transgenesis in farm animals; Brazilian College of Animal Reproduction; Animal Reproduction; 12-2012; 772-776
1806-9614
CONICET Digital
CONICET
url http://hdl.handle.net/11336/272076
identifier_str_mv Salamone, Daniel Felipe; Bevacqua, Romina Jimena; Hiriart, María Inés; Buemo, Carla Paola; Luchetti, Carolina Griselda; et al.; Transgenesis in farm animals; Brazilian College of Animal Reproduction; Animal Reproduction; 12-2012; 772-776
1806-9614
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.cbra.org.br/pages/publicacoes/animalreproduction/issues/download/v9n4/p772-776.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Brazilian College of Animal Reproduction
publisher.none.fl_str_mv Brazilian College of Animal Reproduction
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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