Functional and genomic characterization of ligilactobacillus salivarius TUCO-L2 isolated from lama glama milk: a promising immunobiotic strain to combat infections

Autores
Quilodrán Vega, Sandra; Albarracín, Leonardo Miguel; Mansilla, Flavia Ivana; Arce, Lorena Paola; Zhou, Binghui; Islam, Md Aminul; Tomokiyo, Mikado; Al Kassaa, Imad; Suda, Yoshihito; Kitazawa, Haruki; Villena, Julio Cesar
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Potential probiotic or immunobiotic effects of lactic acid bacteria (LAB) isolated from the milk of the South American camelid llama (Lama glama) have not been reported in published studies. The aim of the present work was to isolate beneficial LAB from llama milk that can be used as potential probiotics active against bacterial pathogens. LAB strains were isolated from llama milk samples. In vitro functional characterization of the strains was performed by evaluating the resistance against gastrointestinal conditions and inhibition of the pathogen growth. Additionally, the adhesive and immunomodulatory properties of the strains were assessed. The functional studies were complemented with a comparative genomic evaluation and in vivo studies in mice. Ligilactobacillus salivarius TUCO-L2 showed enhanced probiotic/immunobiotic potential compared to that of other tested strains. The TUCO-L2 strain was resistant to pH and high bile salt concentrations and demonstrated antimicrobial activity against Gram-negative intestinal pathogens and adhesion to mucins and epithelial cells. L. salivarius TUCO-L2 modulated the innate immune response triggered by Toll-like receptor (TLR)-4 activation in intestinal epithelial cells. This effect involved differential regulation of the expression of inflammatory cytokines and chemokines mediated by the modulation of the negative regulators of the TLR signaling pathway. Moreover, the TUCO-L2 strain enhanced the resistance of mice to Salmonella infection. This is the first report on the isolation and characterization of a potential probiotic/immunobiotic strain from llama milk. The in vitro, in vivo, and in silico investigation performed in this study reveals several research directions that are needed to characterize the TUCO-L2 strain in detail to position this strain as a probiotic or immunobiotic that can be used against infections in humans or animals, including llama.
Fil: Quilodrán Vega, Sandra. Universidad de Concepción; Chile
Fil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología; Argentina. Tohoku University; Japón
Fil: Mansilla, Flavia Ivana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón
Fil: Arce, Lorena Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Tohoku University; Japón
Fil: Zhou, Binghui. Tohoku University; Japón
Fil: Islam, Md Aminul. Tohoku University; Japón. Bangladesh Agricultural University; Bangladesh
Fil: Tomokiyo, Mikado. Tohoku University; Japón
Fil: Al Kassaa, Imad. Lebanese University. Faculty of Public Health; Líbano
Fil: Suda, Yoshihito. Miyagi University; Japón
Fil: Kitazawa, Haruki. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón
Materia
BACTERIAL INFECTION
IMMUNOMODULATION
INTESTINAL EPITHELIA CELL
LAMA GLAMA MILK
LIGILACTOBACILLUS SALIVARIUS TUCO-L2
PROBIOTIC
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/131643
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/131643
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Functional and genomic characterization of ligilactobacillus salivarius TUCO-L2 isolated from lama glama milk: a promising immunobiotic strain to combat infectionsQuilodrán Vega, SandraAlbarracín, Leonardo MiguelMansilla, Flavia IvanaArce, Lorena PaolaZhou, BinghuiIslam, Md AminulTomokiyo, MikadoAl Kassaa, ImadSuda, YoshihitoKitazawa, HarukiVillena, Julio CesarBACTERIAL INFECTIONIMMUNOMODULATIONINTESTINAL EPITHELIA CELLLAMA GLAMA MILKLIGILACTOBACILLUS SALIVARIUS TUCO-L2PROBIOTIChttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Potential probiotic or immunobiotic effects of lactic acid bacteria (LAB) isolated from the milk of the South American camelid llama (Lama glama) have not been reported in published studies. The aim of the present work was to isolate beneficial LAB from llama milk that can be used as potential probiotics active against bacterial pathogens. LAB strains were isolated from llama milk samples. In vitro functional characterization of the strains was performed by evaluating the resistance against gastrointestinal conditions and inhibition of the pathogen growth. Additionally, the adhesive and immunomodulatory properties of the strains were assessed. The functional studies were complemented with a comparative genomic evaluation and in vivo studies in mice. Ligilactobacillus salivarius TUCO-L2 showed enhanced probiotic/immunobiotic potential compared to that of other tested strains. The TUCO-L2 strain was resistant to pH and high bile salt concentrations and demonstrated antimicrobial activity against Gram-negative intestinal pathogens and adhesion to mucins and epithelial cells. L. salivarius TUCO-L2 modulated the innate immune response triggered by Toll-like receptor (TLR)-4 activation in intestinal epithelial cells. This effect involved differential regulation of the expression of inflammatory cytokines and chemokines mediated by the modulation of the negative regulators of the TLR signaling pathway. Moreover, the TUCO-L2 strain enhanced the resistance of mice to Salmonella infection. This is the first report on the isolation and characterization of a potential probiotic/immunobiotic strain from llama milk. The in vitro, in vivo, and in silico investigation performed in this study reveals several research directions that are needed to characterize the TUCO-L2 strain in detail to position this strain as a probiotic or immunobiotic that can be used against infections in humans or animals, including llama.Fil: Quilodrán Vega, Sandra. Universidad de Concepción; ChileFil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología; Argentina. Tohoku University; JapónFil: Mansilla, Flavia Ivana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; JapónFil: Arce, Lorena Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Tohoku University; JapónFil: Zhou, Binghui. Tohoku University; JapónFil: Islam, Md Aminul. Tohoku University; Japón. Bangladesh Agricultural University; BangladeshFil: Tomokiyo, Mikado. Tohoku University; JapónFil: Al Kassaa, Imad. Lebanese University. Faculty of Public Health; LíbanoFil: Suda, Yoshihito. Miyagi University; JapónFil: Kitazawa, Haruki. Tohoku University; JapónFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; JapónFrontiers Media S.A.2020-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/131643Quilodrán Vega, Sandra; Albarracín, Leonardo Miguel; Mansilla, Flavia Ivana; Arce, Lorena Paola; Zhou, Binghui; et al.; Functional and genomic characterization of ligilactobacillus salivarius TUCO-L2 isolated from lama glama milk: a promising immunobiotic strain to combat infections; Frontiers Media S.A.; Frontiers in Microbiology; 11; 12-2020; 1-24; 6087521664-302X1664-302XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2020.608752info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fmicb.2020.608752info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:26:02Zoai:ri.conicet.gov.ar:11336/131643instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:26:02.355CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Functional and genomic characterization of ligilactobacillus salivarius TUCO-L2 isolated from lama glama milk: a promising immunobiotic strain to combat infections
title Functional and genomic characterization of ligilactobacillus salivarius TUCO-L2 isolated from lama glama milk: a promising immunobiotic strain to combat infections
spellingShingle Functional and genomic characterization of ligilactobacillus salivarius TUCO-L2 isolated from lama glama milk: a promising immunobiotic strain to combat infections
Quilodrán Vega, Sandra
BACTERIAL INFECTION
IMMUNOMODULATION
INTESTINAL EPITHELIA CELL
LAMA GLAMA MILK
LIGILACTOBACILLUS SALIVARIUS TUCO-L2
PROBIOTIC
title_short Functional and genomic characterization of ligilactobacillus salivarius TUCO-L2 isolated from lama glama milk: a promising immunobiotic strain to combat infections
title_full Functional and genomic characterization of ligilactobacillus salivarius TUCO-L2 isolated from lama glama milk: a promising immunobiotic strain to combat infections
title_fullStr Functional and genomic characterization of ligilactobacillus salivarius TUCO-L2 isolated from lama glama milk: a promising immunobiotic strain to combat infections
title_full_unstemmed Functional and genomic characterization of ligilactobacillus salivarius TUCO-L2 isolated from lama glama milk: a promising immunobiotic strain to combat infections
title_sort Functional and genomic characterization of ligilactobacillus salivarius TUCO-L2 isolated from lama glama milk: a promising immunobiotic strain to combat infections
dc.creator.none.fl_str_mv Quilodrán Vega, Sandra
Albarracín, Leonardo Miguel
Mansilla, Flavia Ivana
Arce, Lorena Paola
Zhou, Binghui
Islam, Md Aminul
Tomokiyo, Mikado
Al Kassaa, Imad
Suda, Yoshihito
Kitazawa, Haruki
Villena, Julio Cesar
author Quilodrán Vega, Sandra
author_facet Quilodrán Vega, Sandra
Albarracín, Leonardo Miguel
Mansilla, Flavia Ivana
Arce, Lorena Paola
Zhou, Binghui
Islam, Md Aminul
Tomokiyo, Mikado
Al Kassaa, Imad
Suda, Yoshihito
Kitazawa, Haruki
Villena, Julio Cesar
author_role author
author2 Albarracín, Leonardo Miguel
Mansilla, Flavia Ivana
Arce, Lorena Paola
Zhou, Binghui
Islam, Md Aminul
Tomokiyo, Mikado
Al Kassaa, Imad
Suda, Yoshihito
Kitazawa, Haruki
Villena, Julio Cesar
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BACTERIAL INFECTION
IMMUNOMODULATION
INTESTINAL EPITHELIA CELL
LAMA GLAMA MILK
LIGILACTOBACILLUS SALIVARIUS TUCO-L2
PROBIOTIC
topic BACTERIAL INFECTION
IMMUNOMODULATION
INTESTINAL EPITHELIA CELL
LAMA GLAMA MILK
LIGILACTOBACILLUS SALIVARIUS TUCO-L2
PROBIOTIC
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Potential probiotic or immunobiotic effects of lactic acid bacteria (LAB) isolated from the milk of the South American camelid llama (Lama glama) have not been reported in published studies. The aim of the present work was to isolate beneficial LAB from llama milk that can be used as potential probiotics active against bacterial pathogens. LAB strains were isolated from llama milk samples. In vitro functional characterization of the strains was performed by evaluating the resistance against gastrointestinal conditions and inhibition of the pathogen growth. Additionally, the adhesive and immunomodulatory properties of the strains were assessed. The functional studies were complemented with a comparative genomic evaluation and in vivo studies in mice. Ligilactobacillus salivarius TUCO-L2 showed enhanced probiotic/immunobiotic potential compared to that of other tested strains. The TUCO-L2 strain was resistant to pH and high bile salt concentrations and demonstrated antimicrobial activity against Gram-negative intestinal pathogens and adhesion to mucins and epithelial cells. L. salivarius TUCO-L2 modulated the innate immune response triggered by Toll-like receptor (TLR)-4 activation in intestinal epithelial cells. This effect involved differential regulation of the expression of inflammatory cytokines and chemokines mediated by the modulation of the negative regulators of the TLR signaling pathway. Moreover, the TUCO-L2 strain enhanced the resistance of mice to Salmonella infection. This is the first report on the isolation and characterization of a potential probiotic/immunobiotic strain from llama milk. The in vitro, in vivo, and in silico investigation performed in this study reveals several research directions that are needed to characterize the TUCO-L2 strain in detail to position this strain as a probiotic or immunobiotic that can be used against infections in humans or animals, including llama.
Fil: Quilodrán Vega, Sandra. Universidad de Concepción; Chile
Fil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología; Argentina. Tohoku University; Japón
Fil: Mansilla, Flavia Ivana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón
Fil: Arce, Lorena Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Tohoku University; Japón
Fil: Zhou, Binghui. Tohoku University; Japón
Fil: Islam, Md Aminul. Tohoku University; Japón. Bangladesh Agricultural University; Bangladesh
Fil: Tomokiyo, Mikado. Tohoku University; Japón
Fil: Al Kassaa, Imad. Lebanese University. Faculty of Public Health; Líbano
Fil: Suda, Yoshihito. Miyagi University; Japón
Fil: Kitazawa, Haruki. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón
description Potential probiotic or immunobiotic effects of lactic acid bacteria (LAB) isolated from the milk of the South American camelid llama (Lama glama) have not been reported in published studies. The aim of the present work was to isolate beneficial LAB from llama milk that can be used as potential probiotics active against bacterial pathogens. LAB strains were isolated from llama milk samples. In vitro functional characterization of the strains was performed by evaluating the resistance against gastrointestinal conditions and inhibition of the pathogen growth. Additionally, the adhesive and immunomodulatory properties of the strains were assessed. The functional studies were complemented with a comparative genomic evaluation and in vivo studies in mice. Ligilactobacillus salivarius TUCO-L2 showed enhanced probiotic/immunobiotic potential compared to that of other tested strains. The TUCO-L2 strain was resistant to pH and high bile salt concentrations and demonstrated antimicrobial activity against Gram-negative intestinal pathogens and adhesion to mucins and epithelial cells. L. salivarius TUCO-L2 modulated the innate immune response triggered by Toll-like receptor (TLR)-4 activation in intestinal epithelial cells. This effect involved differential regulation of the expression of inflammatory cytokines and chemokines mediated by the modulation of the negative regulators of the TLR signaling pathway. Moreover, the TUCO-L2 strain enhanced the resistance of mice to Salmonella infection. This is the first report on the isolation and characterization of a potential probiotic/immunobiotic strain from llama milk. The in vitro, in vivo, and in silico investigation performed in this study reveals several research directions that are needed to characterize the TUCO-L2 strain in detail to position this strain as a probiotic or immunobiotic that can be used against infections in humans or animals, including llama.
publishDate 2020
dc.date.none.fl_str_mv 2020-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/131643
Quilodrán Vega, Sandra; Albarracín, Leonardo Miguel; Mansilla, Flavia Ivana; Arce, Lorena Paola; Zhou, Binghui; et al.; Functional and genomic characterization of ligilactobacillus salivarius TUCO-L2 isolated from lama glama milk: a promising immunobiotic strain to combat infections; Frontiers Media S.A.; Frontiers in Microbiology; 11; 12-2020; 1-24; 608752
1664-302X
1664-302X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/131643
identifier_str_mv Quilodrán Vega, Sandra; Albarracín, Leonardo Miguel; Mansilla, Flavia Ivana; Arce, Lorena Paola; Zhou, Binghui; et al.; Functional and genomic characterization of ligilactobacillus salivarius TUCO-L2 isolated from lama glama milk: a promising immunobiotic strain to combat infections; Frontiers Media S.A.; Frontiers in Microbiology; 11; 12-2020; 1-24; 608752
1664-302X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2020.608752
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fmicb.2020.608752
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media S.A.
publisher.none.fl_str_mv Frontiers Media S.A.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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