In vitro plant regeneration from cotyledonary nodes of recombinant inbred lines of lentil

Autores
Bermejo, Carolina Julieta; Espósito, María Andrea; Cravero, Vanina Pamela; López Anido, Fernando; Cointry Peix, Enrique Luis
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
An efficient and reproducible in vitro regeneration protocol for lentil was developed. For shoot regeneration, cotyledonary node explants of ten elite genotypes were cultured in an inverted orientation on different shoot regeneration media that consisted of Murashige and Skoog (MS) medium supplemented with 1mgL -1 6-benzylaminopurine (BAP) (M1), 1mgL -1 BAP+0.45mgL -1 indole-3-acetic acid (IAA) (M2), and 2mgL -1 BAP (M3). High percentages of shoot regeneration ranging from 80 to 100% on M1 and M3 media and from 50 to 100% on M2 medium were induced. M1 was the most efficient shoot regeneration medium for most genotypes tested. For rooting, in vitro and in vitro-in vivo methods were used. Low and variable rooting percentages ranging from 0 to 45% were recorded with in vitro-in vivo method. Efficiency of rooting on in vitro medium varied depending on the medium in which shoots had been previously regenerated and the genotype tested. When M1 medium was used, high rooting percentages (over 40%) for most genotypes except for microsperma genotypes were found. When the 10 genotypes were screened for good regeneration performance using M1 medium, 2 main clusters and 3 subgroups within one of the clusters were formed based on similarities respect of the number of regenerated shoots per explant and rooting percentages. Subgroup 1 composed by A1146 genotype produced the highest number of shoots per explant (6.17 shoots) and a high rooting percentage (60%) so was selected for further transformation and use as a potential commercial variety.
Fil: Bermejo, Carolina Julieta. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Departamento de Producción Vegetal. Cátedra de Mejoramiento Vegetal y Producción de Semillas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Espósito, María Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cravero, Vanina Pamela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: López Anido, Fernando. Universidad Nacional de Rosario; Argentina
Fil: Cointry Peix, Enrique Luis. Universidad Nacional de Rosario; Argentina
Materia
COTYLEDONARY NODE
IN VITRO ROOTING
IN VITRO-IN VIVO ROOTING
LENTIL
SHOOT REGENERATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/195928
Acceder
id CONICETDig_02ba7acbae02672548eea27762bc9f2a
oai_identifier_str oai:ri.conicet.gov.ar:11336/195928
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In vitro plant regeneration from cotyledonary nodes of recombinant inbred lines of lentilBermejo, Carolina JulietaEspósito, María AndreaCravero, Vanina PamelaLópez Anido, FernandoCointry Peix, Enrique LuisCOTYLEDONARY NODEIN VITRO ROOTINGIN VITRO-IN VIVO ROOTINGLENTILSHOOT REGENERATIONhttps://purl.org/becyt/ford/4.5https://purl.org/becyt/ford/4An efficient and reproducible in vitro regeneration protocol for lentil was developed. For shoot regeneration, cotyledonary node explants of ten elite genotypes were cultured in an inverted orientation on different shoot regeneration media that consisted of Murashige and Skoog (MS) medium supplemented with 1mgL -1 6-benzylaminopurine (BAP) (M1), 1mgL -1 BAP+0.45mgL -1 indole-3-acetic acid (IAA) (M2), and 2mgL -1 BAP (M3). High percentages of shoot regeneration ranging from 80 to 100% on M1 and M3 media and from 50 to 100% on M2 medium were induced. M1 was the most efficient shoot regeneration medium for most genotypes tested. For rooting, in vitro and in vitro-in vivo methods were used. Low and variable rooting percentages ranging from 0 to 45% were recorded with in vitro-in vivo method. Efficiency of rooting on in vitro medium varied depending on the medium in which shoots had been previously regenerated and the genotype tested. When M1 medium was used, high rooting percentages (over 40%) for most genotypes except for microsperma genotypes were found. When the 10 genotypes were screened for good regeneration performance using M1 medium, 2 main clusters and 3 subgroups within one of the clusters were formed based on similarities respect of the number of regenerated shoots per explant and rooting percentages. Subgroup 1 composed by A1146 genotype produced the highest number of shoots per explant (6.17 shoots) and a high rooting percentage (60%) so was selected for further transformation and use as a potential commercial variety.Fil: Bermejo, Carolina Julieta. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Departamento de Producción Vegetal. Cátedra de Mejoramiento Vegetal y Producción de Semillas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Espósito, María Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cravero, Vanina Pamela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: López Anido, Fernando. Universidad Nacional de Rosario; ArgentinaFil: Cointry Peix, Enrique Luis. Universidad Nacional de Rosario; ArgentinaElsevier Science2012-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/195928Bermejo, Carolina Julieta; Espósito, María Andrea; Cravero, Vanina Pamela; López Anido, Fernando; Cointry Peix, Enrique Luis; In vitro plant regeneration from cotyledonary nodes of recombinant inbred lines of lentil; Elsevier Science; Scientia Horticulturae; 134; 2-2012; 13-190304-4238CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.scienta.2011.11.029info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S030442381100625Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:19:57Zoai:ri.conicet.gov.ar:11336/195928instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:19:57.601CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vitro plant regeneration from cotyledonary nodes of recombinant inbred lines of lentil
title In vitro plant regeneration from cotyledonary nodes of recombinant inbred lines of lentil
spellingShingle In vitro plant regeneration from cotyledonary nodes of recombinant inbred lines of lentil
Bermejo, Carolina Julieta
COTYLEDONARY NODE
IN VITRO ROOTING
IN VITRO-IN VIVO ROOTING
LENTIL
SHOOT REGENERATION
title_short In vitro plant regeneration from cotyledonary nodes of recombinant inbred lines of lentil
title_full In vitro plant regeneration from cotyledonary nodes of recombinant inbred lines of lentil
title_fullStr In vitro plant regeneration from cotyledonary nodes of recombinant inbred lines of lentil
title_full_unstemmed In vitro plant regeneration from cotyledonary nodes of recombinant inbred lines of lentil
title_sort In vitro plant regeneration from cotyledonary nodes of recombinant inbred lines of lentil
dc.creator.none.fl_str_mv Bermejo, Carolina Julieta
Espósito, María Andrea
Cravero, Vanina Pamela
López Anido, Fernando
Cointry Peix, Enrique Luis
author Bermejo, Carolina Julieta
author_facet Bermejo, Carolina Julieta
Espósito, María Andrea
Cravero, Vanina Pamela
López Anido, Fernando
Cointry Peix, Enrique Luis
author_role author
author2 Espósito, María Andrea
Cravero, Vanina Pamela
López Anido, Fernando
Cointry Peix, Enrique Luis
author2_role author
author
author
author
dc.subject.none.fl_str_mv COTYLEDONARY NODE
IN VITRO ROOTING
IN VITRO-IN VIVO ROOTING
LENTIL
SHOOT REGENERATION
topic COTYLEDONARY NODE
IN VITRO ROOTING
IN VITRO-IN VIVO ROOTING
LENTIL
SHOOT REGENERATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.5
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv An efficient and reproducible in vitro regeneration protocol for lentil was developed. For shoot regeneration, cotyledonary node explants of ten elite genotypes were cultured in an inverted orientation on different shoot regeneration media that consisted of Murashige and Skoog (MS) medium supplemented with 1mgL -1 6-benzylaminopurine (BAP) (M1), 1mgL -1 BAP+0.45mgL -1 indole-3-acetic acid (IAA) (M2), and 2mgL -1 BAP (M3). High percentages of shoot regeneration ranging from 80 to 100% on M1 and M3 media and from 50 to 100% on M2 medium were induced. M1 was the most efficient shoot regeneration medium for most genotypes tested. For rooting, in vitro and in vitro-in vivo methods were used. Low and variable rooting percentages ranging from 0 to 45% were recorded with in vitro-in vivo method. Efficiency of rooting on in vitro medium varied depending on the medium in which shoots had been previously regenerated and the genotype tested. When M1 medium was used, high rooting percentages (over 40%) for most genotypes except for microsperma genotypes were found. When the 10 genotypes were screened for good regeneration performance using M1 medium, 2 main clusters and 3 subgroups within one of the clusters were formed based on similarities respect of the number of regenerated shoots per explant and rooting percentages. Subgroup 1 composed by A1146 genotype produced the highest number of shoots per explant (6.17 shoots) and a high rooting percentage (60%) so was selected for further transformation and use as a potential commercial variety.
Fil: Bermejo, Carolina Julieta. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Departamento de Producción Vegetal. Cátedra de Mejoramiento Vegetal y Producción de Semillas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Espósito, María Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cravero, Vanina Pamela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: López Anido, Fernando. Universidad Nacional de Rosario; Argentina
Fil: Cointry Peix, Enrique Luis. Universidad Nacional de Rosario; Argentina
description An efficient and reproducible in vitro regeneration protocol for lentil was developed. For shoot regeneration, cotyledonary node explants of ten elite genotypes were cultured in an inverted orientation on different shoot regeneration media that consisted of Murashige and Skoog (MS) medium supplemented with 1mgL -1 6-benzylaminopurine (BAP) (M1), 1mgL -1 BAP+0.45mgL -1 indole-3-acetic acid (IAA) (M2), and 2mgL -1 BAP (M3). High percentages of shoot regeneration ranging from 80 to 100% on M1 and M3 media and from 50 to 100% on M2 medium were induced. M1 was the most efficient shoot regeneration medium for most genotypes tested. For rooting, in vitro and in vitro-in vivo methods were used. Low and variable rooting percentages ranging from 0 to 45% were recorded with in vitro-in vivo method. Efficiency of rooting on in vitro medium varied depending on the medium in which shoots had been previously regenerated and the genotype tested. When M1 medium was used, high rooting percentages (over 40%) for most genotypes except for microsperma genotypes were found. When the 10 genotypes were screened for good regeneration performance using M1 medium, 2 main clusters and 3 subgroups within one of the clusters were formed based on similarities respect of the number of regenerated shoots per explant and rooting percentages. Subgroup 1 composed by A1146 genotype produced the highest number of shoots per explant (6.17 shoots) and a high rooting percentage (60%) so was selected for further transformation and use as a potential commercial variety.
publishDate 2012
dc.date.none.fl_str_mv 2012-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/195928
Bermejo, Carolina Julieta; Espósito, María Andrea; Cravero, Vanina Pamela; López Anido, Fernando; Cointry Peix, Enrique Luis; In vitro plant regeneration from cotyledonary nodes of recombinant inbred lines of lentil; Elsevier Science; Scientia Horticulturae; 134; 2-2012; 13-19
0304-4238
CONICET Digital
CONICET
url http://hdl.handle.net/11336/195928
identifier_str_mv Bermejo, Carolina Julieta; Espósito, María Andrea; Cravero, Vanina Pamela; López Anido, Fernando; Cointry Peix, Enrique Luis; In vitro plant regeneration from cotyledonary nodes of recombinant inbred lines of lentil; Elsevier Science; Scientia Horticulturae; 134; 2-2012; 13-19
0304-4238
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.scienta.2011.11.029
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S030442381100625X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781683381043200
score 12.8982525

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