Micropropagation of Prosopis chilensis (Mol.) Stuntz from young and mature plants

Autores
Caro, Luis Alberto; Polci, Pablo Aldo; Lindström, Lilia Ivone; Echenique, Carmen Viviana; Hernandez, Luis Francisco
Año de publicación
2002
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Prosopis chilensis (Mol.) Stuntz (Algarrobo de Chile) is an important native tree species that can be grown in arid and semiarid regions for wood and forage production and environmental protection. Developing a simple and reliable in vitro protocol for cloning it would enable to improve it genetically. Explants of P.chilensis were taken from 4 months-old plants grown in the greenhouse or from adult trees grown in a natural environment. Nodal segments 1 ? 2 cm long containing an axillary bud were selected from elongating shoots. These cuttings were aseptically cultured on two agar-solid basal media, MS or BTMm, and treated with 0.05 mg L-1 BA and 3 mg L-1 of either IAA, IBA or NAA. Sucrose (3% w/v) was used as carbon source. The percentage of sprouted cuttings and whole plant regeneration as well as its shoot and root length were recorded. Number, length and dry weight of shoots and roots were also measured. Rooting was successfulwith cuttings taken from young or adult plants, but explants from young plants showed a better response.Culturing in BTMm resulted in significantly greater shoot and root biomass than culturing in MS. Moreover, this response was higher in young explants when IBA was used as growth regulator. This paper reports a simple and effective method to micropropagate P. chilensis from young and adult plants.
Fil: Caro, Luis Alberto. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Polci, Pablo Aldo. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Lindström, Lilia Ivone. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Echenique, Carmen Viviana. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Hernandez, Luis Francisco. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Materia
ALGARROBO DE CHILE
IN VITRO CULTURE
PROSOPIS CHILENSIS
REGENERATION
ROOTING
ALGARROBO DE CHILE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/32659
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Micropropagation of Prosopis chilensis (Mol.) Stuntz from young and mature plantsCaro, Luis AlbertoPolci, Pablo AldoLindström, Lilia IvoneEchenique, Carmen VivianaHernandez, Luis FranciscoALGARROBO DE CHILEIN VITRO CULTUREPROSOPIS CHILENSISREGENERATIONROOTINGALGARROBO DE CHILEhttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4https://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Prosopis chilensis (Mol.) Stuntz (Algarrobo de Chile) is an important native tree species that can be grown in arid and semiarid regions for wood and forage production and environmental protection. Developing a simple and reliable in vitro protocol for cloning it would enable to improve it genetically. Explants of P.chilensis were taken from 4 months-old plants grown in the greenhouse or from adult trees grown in a natural environment. Nodal segments 1 ? 2 cm long containing an axillary bud were selected from elongating shoots. These cuttings were aseptically cultured on two agar-solid basal media, MS or BTMm, and treated with 0.05 mg L-1 BA and 3 mg L-1 of either IAA, IBA or NAA. Sucrose (3% w/v) was used as carbon source. The percentage of sprouted cuttings and whole plant regeneration as well as its shoot and root length were recorded. Number, length and dry weight of shoots and roots were also measured. Rooting was successfulwith cuttings taken from young or adult plants, but explants from young plants showed a better response.Culturing in BTMm resulted in significantly greater shoot and root biomass than culturing in MS. Moreover, this response was higher in young explants when IBA was used as growth regulator. This paper reports a simple and effective method to micropropagate P. chilensis from young and adult plants.Fil: Caro, Luis Alberto. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Polci, Pablo Aldo. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Lindström, Lilia Ivone. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Echenique, Carmen Viviana. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Hernandez, Luis Francisco. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaInstituto de Histología y Embriología2002-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/32659Echenique, Carmen Viviana; Lindström, Lilia Ivone; Polci, Pablo Aldo; Caro, Luis Alberto; Hernandez, Luis Francisco; Micropropagation of Prosopis chilensis (Mol.) Stuntz from young and mature plants; Instituto de Histología y Embriología; Biocell; 26; 1; 4-2002; 25-330327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.cricyt.edu.ar/biocell/vol/pdf/26/03.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:06Zoai:ri.conicet.gov.ar:11336/32659instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:07.133CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Micropropagation of Prosopis chilensis (Mol.) Stuntz from young and mature plants
title Micropropagation of Prosopis chilensis (Mol.) Stuntz from young and mature plants
spellingShingle Micropropagation of Prosopis chilensis (Mol.) Stuntz from young and mature plants
Caro, Luis Alberto
ALGARROBO DE CHILE
IN VITRO CULTURE
PROSOPIS CHILENSIS
REGENERATION
ROOTING
ALGARROBO DE CHILE
title_short Micropropagation of Prosopis chilensis (Mol.) Stuntz from young and mature plants
title_full Micropropagation of Prosopis chilensis (Mol.) Stuntz from young and mature plants
title_fullStr Micropropagation of Prosopis chilensis (Mol.) Stuntz from young and mature plants
title_full_unstemmed Micropropagation of Prosopis chilensis (Mol.) Stuntz from young and mature plants
title_sort Micropropagation of Prosopis chilensis (Mol.) Stuntz from young and mature plants
dc.creator.none.fl_str_mv Caro, Luis Alberto
Polci, Pablo Aldo
Lindström, Lilia Ivone
Echenique, Carmen Viviana
Hernandez, Luis Francisco
author Caro, Luis Alberto
author_facet Caro, Luis Alberto
Polci, Pablo Aldo
Lindström, Lilia Ivone
Echenique, Carmen Viviana
Hernandez, Luis Francisco
author_role author
author2 Polci, Pablo Aldo
Lindström, Lilia Ivone
Echenique, Carmen Viviana
Hernandez, Luis Francisco
author2_role author
author
author
author
dc.subject.none.fl_str_mv ALGARROBO DE CHILE
IN VITRO CULTURE
PROSOPIS CHILENSIS
REGENERATION
ROOTING
ALGARROBO DE CHILE
topic ALGARROBO DE CHILE
IN VITRO CULTURE
PROSOPIS CHILENSIS
REGENERATION
ROOTING
ALGARROBO DE CHILE
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Prosopis chilensis (Mol.) Stuntz (Algarrobo de Chile) is an important native tree species that can be grown in arid and semiarid regions for wood and forage production and environmental protection. Developing a simple and reliable in vitro protocol for cloning it would enable to improve it genetically. Explants of P.chilensis were taken from 4 months-old plants grown in the greenhouse or from adult trees grown in a natural environment. Nodal segments 1 ? 2 cm long containing an axillary bud were selected from elongating shoots. These cuttings were aseptically cultured on two agar-solid basal media, MS or BTMm, and treated with 0.05 mg L-1 BA and 3 mg L-1 of either IAA, IBA or NAA. Sucrose (3% w/v) was used as carbon source. The percentage of sprouted cuttings and whole plant regeneration as well as its shoot and root length were recorded. Number, length and dry weight of shoots and roots were also measured. Rooting was successfulwith cuttings taken from young or adult plants, but explants from young plants showed a better response.Culturing in BTMm resulted in significantly greater shoot and root biomass than culturing in MS. Moreover, this response was higher in young explants when IBA was used as growth regulator. This paper reports a simple and effective method to micropropagate P. chilensis from young and adult plants.
Fil: Caro, Luis Alberto. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Polci, Pablo Aldo. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Lindström, Lilia Ivone. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Echenique, Carmen Viviana. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Hernandez, Luis Francisco. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
description Prosopis chilensis (Mol.) Stuntz (Algarrobo de Chile) is an important native tree species that can be grown in arid and semiarid regions for wood and forage production and environmental protection. Developing a simple and reliable in vitro protocol for cloning it would enable to improve it genetically. Explants of P.chilensis were taken from 4 months-old plants grown in the greenhouse or from adult trees grown in a natural environment. Nodal segments 1 ? 2 cm long containing an axillary bud were selected from elongating shoots. These cuttings were aseptically cultured on two agar-solid basal media, MS or BTMm, and treated with 0.05 mg L-1 BA and 3 mg L-1 of either IAA, IBA or NAA. Sucrose (3% w/v) was used as carbon source. The percentage of sprouted cuttings and whole plant regeneration as well as its shoot and root length were recorded. Number, length and dry weight of shoots and roots were also measured. Rooting was successfulwith cuttings taken from young or adult plants, but explants from young plants showed a better response.Culturing in BTMm resulted in significantly greater shoot and root biomass than culturing in MS. Moreover, this response was higher in young explants when IBA was used as growth regulator. This paper reports a simple and effective method to micropropagate P. chilensis from young and adult plants.
publishDate 2002
dc.date.none.fl_str_mv 2002-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/32659
Echenique, Carmen Viviana; Lindström, Lilia Ivone; Polci, Pablo Aldo; Caro, Luis Alberto; Hernandez, Luis Francisco; Micropropagation of Prosopis chilensis (Mol.) Stuntz from young and mature plants; Instituto de Histología y Embriología; Biocell; 26; 1; 4-2002; 25-33
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/32659
identifier_str_mv Echenique, Carmen Viviana; Lindström, Lilia Ivone; Polci, Pablo Aldo; Caro, Luis Alberto; Hernandez, Luis Francisco; Micropropagation of Prosopis chilensis (Mol.) Stuntz from young and mature plants; Instituto de Histología y Embriología; Biocell; 26; 1; 4-2002; 25-33
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.cricyt.edu.ar/biocell/vol/pdf/26/03.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Instituto de Histología y Embriología
publisher.none.fl_str_mv Instituto de Histología y Embriología
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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