Eucalyptus dunnii Maiden plant regeneration via shoot organogenesis on a new basal medium based on the mineral composition of young stump shoots

Authors
Oberschelp, Gustavo Pedro Javier; Gonçalves, Antônio Natal; Meneghetti, Eveline Calderan; Graner, Érika Mendes; Almeida, Marcílio de
Publication Year
2015
Language
English
Format
article
Status
Published version
Description
Eucalyptus dunnii is an excellent alternative for afforestation and reforestation in frost-prone areas; however, it has limitations in the availability of propagation material. Given the great potential of tissue culture techniques for plant propagation and plant genetic transformation, the competence for in vitro organogenesis from E. dunnii cotyledons and hypocotyls was assessed. Several combinations of 6-benzylaminopurine (BAP) plus α-naphthalene acetic acid (NAA) or indole-3-acetic acid (IAA) were added (0, 0.1, 0.5, and 1 mg l−1) to a specifically developed basal medium. Moreover, the effect of the hypocotyl segments position (distal and proximal) on bud and callus regeneration was studied. Finally, microcuttings obtained from in vitro multiplication of the regenerated buds were treated with different dosages of indole-3-butyric acid (IBA) or NAA (powder based) and rooted under ex vitro conditions. Bud regeneration was observed only in hypocotyl segments, where dosages of 0.5 mg l−1 BAP plus 0.5 mg l−1 NAA or 0.5 mg l−1 IAA and distal segments resulted in higher bud regeneration rates. Histological analyses suggest that callus regeneration areas in hypocotyl explants recreate an appropriate niche for the development of pluripotent cells that give rise to buds and shoots. Either IBA or NAA treatments failed to significantly improved the ex vitro rooting of E. dunnii microcuttings, whereas >50% microcuttings rooted without application of growth regulators.
EEA Concordia
Fil: Oberschelp, Gustavo Pedro Javier. Universidade de São Paulo. Escola Superior de Agricultura “Luiz de Queiroz”, Depto. de Ciências Florestais, PPG em Recursos Florestais; Brasil. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Gonçalves, Antônio Natal. Universidade de São Paulo. Escola Superior de Agricultura “Luiz de Queiroz”, Depto. de Ciências Florestais, PPG em Recursos Florestais; Brasil
Fil: Meneghetti, Eveline Calderan. Universidade de São Paulo. Escola Superior de Agricultura “Luiz de Queiroz”. Depto. de Ciências Biológicas. PPG em Fisiologia e Bioquímica de Plantas; Brasil
Fil: Graner, Érika Mendes. Universidade de São Paulo. Escola Superior de Agricultura “Luiz de Queiroz”. Depto. de Ciências Biológicas. PPG em Fisiologia e Bioquímica de Plantas; Brasil
Fil: Almeida, Marcílio de. Universidade de São Paulo. Escola Superior de Agricultura “Luiz de Queiroz”, Depto. de Ciências Florestais, PPG em Recursos Florestais; Brasil. Universidade de São Paulo. Escola Superior de Agricultura “Luiz de Queiroz”. Depto. de Ciências Biológicas. PPG em Fisiologia e Bioquímica de Plantas; Brasil
Source
In vitro cellular & developmental biology - Plant 51 (6) : 626–636. (December 2015)
Subject
Eucalyptus
Regeneración
Regeneración in Vitro
Enraizamiento
Organogénesis
Regeneration
In Vitro Regeneration
Rooting
Organogenesis
Eucalyptus dunnii
Propagación in vitro
Access level
Restricted access
License
Repository
INTA Digital (INTA)
Institution
Instituto Nacional de Tecnología Agropecuaria
OAI Identifier
oai:localhost:20.500.12123/2623