Eucalyptus dunnii Maiden plant regeneration via shoot organogenesis on a new basal medium based on the mineral composition of young stump shoots

Autores
Oberschelp, Gustavo Pedro Javier; Gonçalves, Antônio Natal; Meneghetti, Eveline Calderan; Graner, Érika Mendes; Almeida, Marcílio de
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Eucalyptus dunnii is an excellent alternative for afforestation and reforestation in frost-prone areas; however, it has limitations in the availability of propagation material. Given the great potential of tissue culture techniques for plant propagation and plant genetic transformation, the competence for in vitro organogenesis from E. dunnii cotyledons and hypocotyls was assessed. Several combinations of 6-benzylaminopurine (BAP) plus α-naphthalene acetic acid (NAA) or indole-3-acetic acid (IAA) were added (0, 0.1, 0.5, and 1 mg l−1) to a specifically developed basal medium. Moreover, the effect of the hypocotyl segments position (distal and proximal) on bud and callus regeneration was studied. Finally, microcuttings obtained from in vitro multiplication of the regenerated buds were treated with different dosages of indole-3-butyric acid (IBA) or NAA (powder based) and rooted under ex vitro conditions. Bud regeneration was observed only in hypocotyl segments, where dosages of 0.5 mg l−1 BAP plus 0.5 mg l−1 NAA or 0.5 mg l−1 IAA and distal segments resulted in higher bud regeneration rates. Histological analyses suggest that callus regeneration areas in hypocotyl explants recreate an appropriate niche for the development of pluripotent cells that give rise to buds and shoots. Either IBA or NAA treatments failed to significantly improved the ex vitro rooting of E. dunnii microcuttings, whereas >50% microcuttings rooted without application of growth regulators.
EEA Concordia
Fil: Oberschelp, Gustavo Pedro Javier. Universidade de São Paulo. Escola Superior de Agricultura “Luiz de Queiroz”, Depto. de Ciências Florestais, PPG em Recursos Florestais; Brasil. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Gonçalves, Antônio Natal. Universidade de São Paulo. Escola Superior de Agricultura “Luiz de Queiroz”, Depto. de Ciências Florestais, PPG em Recursos Florestais; Brasil
Fil: Meneghetti, Eveline Calderan. Universidade de São Paulo. Escola Superior de Agricultura “Luiz de Queiroz”. Depto. de Ciências Biológicas. PPG em Fisiologia e Bioquímica de Plantas; Brasil
Fil: Graner, Érika Mendes. Universidade de São Paulo. Escola Superior de Agricultura “Luiz de Queiroz”. Depto. de Ciências Biológicas. PPG em Fisiologia e Bioquímica de Plantas; Brasil
Fil: Almeida, Marcílio de. Universidade de São Paulo. Escola Superior de Agricultura “Luiz de Queiroz”, Depto. de Ciências Florestais, PPG em Recursos Florestais; Brasil. Universidade de São Paulo. Escola Superior de Agricultura “Luiz de Queiroz”. Depto. de Ciências Biológicas. PPG em Fisiologia e Bioquímica de Plantas; Brasil
Fuente
In vitro cellular & developmental biology - Plant 51 (6) : 626–636. (December 2015)
Materia
Eucalyptus
Regeneración
Regeneración in Vitro
Enraizamiento
Organogénesis
Regeneration
In Vitro Regeneration
Rooting
Organogenesis
Eucalyptus dunnii
Propagación in vitro
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/2623
Acceder
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oai_identifier_str oai:localhost:20.500.12123/2623
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Eucalyptus dunnii Maiden plant regeneration via shoot organogenesis on a new basal medium based on the mineral composition of young stump shootsOberschelp, Gustavo Pedro JavierGonçalves, Antônio NatalMeneghetti, Eveline CalderanGraner, Érika MendesAlmeida, Marcílio deEucalyptusRegeneraciónRegeneración in VitroEnraizamientoOrganogénesisRegenerationIn Vitro RegenerationRootingOrganogenesisEucalyptus dunniiPropagación in vitroEucalyptus dunnii is an excellent alternative for afforestation and reforestation in frost-prone areas; however, it has limitations in the availability of propagation material. Given the great potential of tissue culture techniques for plant propagation and plant genetic transformation, the competence for in vitro organogenesis from E. dunnii cotyledons and hypocotyls was assessed. Several combinations of 6-benzylaminopurine (BAP) plus α-naphthalene acetic acid (NAA) or indole-3-acetic acid (IAA) were added (0, 0.1, 0.5, and 1 mg l−1) to a specifically developed basal medium. Moreover, the effect of the hypocotyl segments position (distal and proximal) on bud and callus regeneration was studied. Finally, microcuttings obtained from in vitro multiplication of the regenerated buds were treated with different dosages of indole-3-butyric acid (IBA) or NAA (powder based) and rooted under ex vitro conditions. Bud regeneration was observed only in hypocotyl segments, where dosages of 0.5 mg l−1 BAP plus 0.5 mg l−1 NAA or 0.5 mg l−1 IAA and distal segments resulted in higher bud regeneration rates. Histological analyses suggest that callus regeneration areas in hypocotyl explants recreate an appropriate niche for the development of pluripotent cells that give rise to buds and shoots. Either IBA or NAA treatments failed to significantly improved the ex vitro rooting of E. dunnii microcuttings, whereas >50% microcuttings rooted without application of growth regulators.EEA ConcordiaFil: Oberschelp, Gustavo Pedro Javier. Universidade de São Paulo. Escola Superior de Agricultura “Luiz de Queiroz”, Depto. de Ciências Florestais, PPG em Recursos Florestais; Brasil. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; ArgentinaFil: Gonçalves, Antônio Natal. Universidade de São Paulo. Escola Superior de Agricultura “Luiz de Queiroz”, Depto. de Ciências Florestais, PPG em Recursos Florestais; BrasilFil: Meneghetti, Eveline Calderan. Universidade de São Paulo. Escola Superior de Agricultura “Luiz de Queiroz”. Depto. de Ciências Biológicas. PPG em Fisiologia e Bioquímica de Plantas; BrasilFil: Graner, Érika Mendes. Universidade de São Paulo. Escola Superior de Agricultura “Luiz de Queiroz”. Depto. de Ciências Biológicas. PPG em Fisiologia e Bioquímica de Plantas; BrasilFil: Almeida, Marcílio de. Universidade de São Paulo. Escola Superior de Agricultura “Luiz de Queiroz”, Depto. de Ciências Florestais, PPG em Recursos Florestais; Brasil. Universidade de São Paulo. Escola Superior de Agricultura “Luiz de Queiroz”. Depto. de Ciências Biológicas. PPG em Fisiologia e Bioquímica de Plantas; Brasil2018-06-14T13:34:47Z2018-06-14T13:34:47Z2015-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://link.springer.com/article/10.1007/s11627-015-9715-8http://hdl.handle.net/20.500.12123/26231054-54761475-2689https://doi.org/10.1007/s11627-015-9715-8In vitro cellular & developmental biology - Plant 51 (6) : 626–636. (December 2015)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:19Zoai:localhost:20.500.12123/2623instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:20.496INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Eucalyptus dunnii Maiden plant regeneration via shoot organogenesis on a new basal medium based on the mineral composition of young stump shoots
title Eucalyptus dunnii Maiden plant regeneration via shoot organogenesis on a new basal medium based on the mineral composition of young stump shoots
spellingShingle Eucalyptus dunnii Maiden plant regeneration via shoot organogenesis on a new basal medium based on the mineral composition of young stump shoots
Oberschelp, Gustavo Pedro Javier
Eucalyptus
Regeneración
Regeneración in Vitro
Enraizamiento
Organogénesis
Regeneration
In Vitro Regeneration
Rooting
Organogenesis
Eucalyptus dunnii
Propagación in vitro
title_short Eucalyptus dunnii Maiden plant regeneration via shoot organogenesis on a new basal medium based on the mineral composition of young stump shoots
title_full Eucalyptus dunnii Maiden plant regeneration via shoot organogenesis on a new basal medium based on the mineral composition of young stump shoots
title_fullStr Eucalyptus dunnii Maiden plant regeneration via shoot organogenesis on a new basal medium based on the mineral composition of young stump shoots
title_full_unstemmed Eucalyptus dunnii Maiden plant regeneration via shoot organogenesis on a new basal medium based on the mineral composition of young stump shoots
title_sort Eucalyptus dunnii Maiden plant regeneration via shoot organogenesis on a new basal medium based on the mineral composition of young stump shoots
dc.creator.none.fl_str_mv Oberschelp, Gustavo Pedro Javier
Gonçalves, Antônio Natal
Meneghetti, Eveline Calderan
Graner, Érika Mendes
Almeida, Marcílio de
author Oberschelp, Gustavo Pedro Javier
author_facet Oberschelp, Gustavo Pedro Javier
Gonçalves, Antônio Natal
Meneghetti, Eveline Calderan
Graner, Érika Mendes
Almeida, Marcílio de
author_role author
author2 Gonçalves, Antônio Natal
Meneghetti, Eveline Calderan
Graner, Érika Mendes
Almeida, Marcílio de
author2_role author
author
author
author
dc.subject.none.fl_str_mv Eucalyptus
Regeneración
Regeneración in Vitro
Enraizamiento
Organogénesis
Regeneration
In Vitro Regeneration
Rooting
Organogenesis
Eucalyptus dunnii
Propagación in vitro
topic Eucalyptus
Regeneración
Regeneración in Vitro
Enraizamiento
Organogénesis
Regeneration
In Vitro Regeneration
Rooting
Organogenesis
Eucalyptus dunnii
Propagación in vitro
dc.description.none.fl_txt_mv Eucalyptus dunnii is an excellent alternative for afforestation and reforestation in frost-prone areas; however, it has limitations in the availability of propagation material. Given the great potential of tissue culture techniques for plant propagation and plant genetic transformation, the competence for in vitro organogenesis from E. dunnii cotyledons and hypocotyls was assessed. Several combinations of 6-benzylaminopurine (BAP) plus α-naphthalene acetic acid (NAA) or indole-3-acetic acid (IAA) were added (0, 0.1, 0.5, and 1 mg l−1) to a specifically developed basal medium. Moreover, the effect of the hypocotyl segments position (distal and proximal) on bud and callus regeneration was studied. Finally, microcuttings obtained from in vitro multiplication of the regenerated buds were treated with different dosages of indole-3-butyric acid (IBA) or NAA (powder based) and rooted under ex vitro conditions. Bud regeneration was observed only in hypocotyl segments, where dosages of 0.5 mg l−1 BAP plus 0.5 mg l−1 NAA or 0.5 mg l−1 IAA and distal segments resulted in higher bud regeneration rates. Histological analyses suggest that callus regeneration areas in hypocotyl explants recreate an appropriate niche for the development of pluripotent cells that give rise to buds and shoots. Either IBA or NAA treatments failed to significantly improved the ex vitro rooting of E. dunnii microcuttings, whereas >50% microcuttings rooted without application of growth regulators.
EEA Concordia
Fil: Oberschelp, Gustavo Pedro Javier. Universidade de São Paulo. Escola Superior de Agricultura “Luiz de Queiroz”, Depto. de Ciências Florestais, PPG em Recursos Florestais; Brasil. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Gonçalves, Antônio Natal. Universidade de São Paulo. Escola Superior de Agricultura “Luiz de Queiroz”, Depto. de Ciências Florestais, PPG em Recursos Florestais; Brasil
Fil: Meneghetti, Eveline Calderan. Universidade de São Paulo. Escola Superior de Agricultura “Luiz de Queiroz”. Depto. de Ciências Biológicas. PPG em Fisiologia e Bioquímica de Plantas; Brasil
Fil: Graner, Érika Mendes. Universidade de São Paulo. Escola Superior de Agricultura “Luiz de Queiroz”. Depto. de Ciências Biológicas. PPG em Fisiologia e Bioquímica de Plantas; Brasil
Fil: Almeida, Marcílio de. Universidade de São Paulo. Escola Superior de Agricultura “Luiz de Queiroz”, Depto. de Ciências Florestais, PPG em Recursos Florestais; Brasil. Universidade de São Paulo. Escola Superior de Agricultura “Luiz de Queiroz”. Depto. de Ciências Biológicas. PPG em Fisiologia e Bioquímica de Plantas; Brasil
description Eucalyptus dunnii is an excellent alternative for afforestation and reforestation in frost-prone areas; however, it has limitations in the availability of propagation material. Given the great potential of tissue culture techniques for plant propagation and plant genetic transformation, the competence for in vitro organogenesis from E. dunnii cotyledons and hypocotyls was assessed. Several combinations of 6-benzylaminopurine (BAP) plus α-naphthalene acetic acid (NAA) or indole-3-acetic acid (IAA) were added (0, 0.1, 0.5, and 1 mg l−1) to a specifically developed basal medium. Moreover, the effect of the hypocotyl segments position (distal and proximal) on bud and callus regeneration was studied. Finally, microcuttings obtained from in vitro multiplication of the regenerated buds were treated with different dosages of indole-3-butyric acid (IBA) or NAA (powder based) and rooted under ex vitro conditions. Bud regeneration was observed only in hypocotyl segments, where dosages of 0.5 mg l−1 BAP plus 0.5 mg l−1 NAA or 0.5 mg l−1 IAA and distal segments resulted in higher bud regeneration rates. Histological analyses suggest that callus regeneration areas in hypocotyl explants recreate an appropriate niche for the development of pluripotent cells that give rise to buds and shoots. Either IBA or NAA treatments failed to significantly improved the ex vitro rooting of E. dunnii microcuttings, whereas >50% microcuttings rooted without application of growth regulators.
publishDate 2015
dc.date.none.fl_str_mv 2015-12
2018-06-14T13:34:47Z
2018-06-14T13:34:47Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://link.springer.com/article/10.1007/s11627-015-9715-8
http://hdl.handle.net/20.500.12123/2623
1054-5476
1475-2689
https://doi.org/10.1007/s11627-015-9715-8
url https://link.springer.com/article/10.1007/s11627-015-9715-8
http://hdl.handle.net/20.500.12123/2623
https://doi.org/10.1007/s11627-015-9715-8
identifier_str_mv 1054-5476
1475-2689
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv In vitro cellular & developmental biology - Plant 51 (6) : 626–636. (December 2015)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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