In vitro embryo culture to shorten the breeding cycle in lentil (Lens culinaris Medik)

Autores
Bermejo, Carolina Julieta; Gatti, Ileana; Cointry Peix, Enrique Luis
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Breeding in lentil involves hybridization followed by different selection methods and requires 10 years to obtain a cultivar, as only one field generation per year can be produced. To shorten the breeding time it is essential to use biotechnological methods such as in vitro embryo culture combined with SSD method since only one seed is enough to produce the next generation. An efficient in vitro–in vivo system was developed. The best time to extract immature embryos and the best culture medium to obtain their complete development were analyzed. Embryos of Pardina, B1181 (microsperma type), B1051 and A1145 (macrosperma type) were collected at 15, 18, 21, and 24 days after pollination (DAP) and cultured on MS medium with five different concentrations of 6-benzylaminopurine (BAP) (0–0.025–0.05–0.1–0.25 mg L−1). An ANOVA test among genotypes, media, DAP and their interactions was performed. Genotypes, DAP and its interaction showed significant effects on the percentage of shoot production (F = 61.8; F = 79.3; F = 8.5; p < 0.01) and germination (F = 70.7; F = 69.8; F = 3.9; p < 0.01). Medium effect was only significant for germination (F = 8.7; p < 0.01). The microsperma genotypes gave higher percentages of shoot production (>80 %) and germination (>70 %). Although in vitro culture efficiency increased with DAP, 18 DAP was selected due to its high percentages of germination (13–70 %). The medium without BAP was the most suitable for embryo complete development (41–87 %). All plants obtained were morphologically normal and fertile. Using this approach, four generations per year were obtained allowing a rapid development of RILs.
Fil: Bermejo, Carolina Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Gatti, Ileana. Consejo de Investigadores de la Universidad Nacional de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina
Fil: Cointry Peix, Enrique Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina
Materia
IMMATURE SEEDS
IN VITRO CULTURE
LENTIL
SHORT GENERATION CYCLES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/52851
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/52851
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In vitro embryo culture to shorten the breeding cycle in lentil (Lens culinaris Medik)Bermejo, Carolina JulietaGatti, IleanaCointry Peix, Enrique LuisIMMATURE SEEDSIN VITRO CULTURELENTILSHORT GENERATION CYCLEShttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Breeding in lentil involves hybridization followed by different selection methods and requires 10 years to obtain a cultivar, as only one field generation per year can be produced. To shorten the breeding time it is essential to use biotechnological methods such as in vitro embryo culture combined with SSD method since only one seed is enough to produce the next generation. An efficient in vitro–in vivo system was developed. The best time to extract immature embryos and the best culture medium to obtain their complete development were analyzed. Embryos of Pardina, B1181 (microsperma type), B1051 and A1145 (macrosperma type) were collected at 15, 18, 21, and 24 days after pollination (DAP) and cultured on MS medium with five different concentrations of 6-benzylaminopurine (BAP) (0–0.025–0.05–0.1–0.25 mg L−1). An ANOVA test among genotypes, media, DAP and their interactions was performed. Genotypes, DAP and its interaction showed significant effects on the percentage of shoot production (F = 61.8; F = 79.3; F = 8.5; p < 0.01) and germination (F = 70.7; F = 69.8; F = 3.9; p < 0.01). Medium effect was only significant for germination (F = 8.7; p < 0.01). The microsperma genotypes gave higher percentages of shoot production (>80 %) and germination (>70 %). Although in vitro culture efficiency increased with DAP, 18 DAP was selected due to its high percentages of germination (13–70 %). The medium without BAP was the most suitable for embryo complete development (41–87 %). All plants obtained were morphologically normal and fertile. Using this approach, four generations per year were obtained allowing a rapid development of RILs.Fil: Bermejo, Carolina Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Gatti, Ileana. Consejo de Investigadores de la Universidad Nacional de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; ArgentinaFil: Cointry Peix, Enrique Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; ArgentinaSpringer2016-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/52851Bermejo, Carolina Julieta; Gatti, Ileana; Cointry Peix, Enrique Luis; In vitro embryo culture to shorten the breeding cycle in lentil (Lens culinaris Medik); Springer; Plant Cell, Tissue and Organ Culture; 127; 3; 12-2016; 585-5900167-6857CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s11240-016-1065-7info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11240-016-1065-7info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:35:58Zoai:ri.conicet.gov.ar:11336/52851instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:35:59.122CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vitro embryo culture to shorten the breeding cycle in lentil (Lens culinaris Medik)
title In vitro embryo culture to shorten the breeding cycle in lentil (Lens culinaris Medik)
spellingShingle In vitro embryo culture to shorten the breeding cycle in lentil (Lens culinaris Medik)
Bermejo, Carolina Julieta
IMMATURE SEEDS
IN VITRO CULTURE
LENTIL
SHORT GENERATION CYCLES
title_short In vitro embryo culture to shorten the breeding cycle in lentil (Lens culinaris Medik)
title_full In vitro embryo culture to shorten the breeding cycle in lentil (Lens culinaris Medik)
title_fullStr In vitro embryo culture to shorten the breeding cycle in lentil (Lens culinaris Medik)
title_full_unstemmed In vitro embryo culture to shorten the breeding cycle in lentil (Lens culinaris Medik)
title_sort In vitro embryo culture to shorten the breeding cycle in lentil (Lens culinaris Medik)
dc.creator.none.fl_str_mv Bermejo, Carolina Julieta
Gatti, Ileana
Cointry Peix, Enrique Luis
author Bermejo, Carolina Julieta
author_facet Bermejo, Carolina Julieta
Gatti, Ileana
Cointry Peix, Enrique Luis
author_role author
author2 Gatti, Ileana
Cointry Peix, Enrique Luis
author2_role author
author
dc.subject.none.fl_str_mv IMMATURE SEEDS
IN VITRO CULTURE
LENTIL
SHORT GENERATION CYCLES
topic IMMATURE SEEDS
IN VITRO CULTURE
LENTIL
SHORT GENERATION CYCLES
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Breeding in lentil involves hybridization followed by different selection methods and requires 10 years to obtain a cultivar, as only one field generation per year can be produced. To shorten the breeding time it is essential to use biotechnological methods such as in vitro embryo culture combined with SSD method since only one seed is enough to produce the next generation. An efficient in vitro–in vivo system was developed. The best time to extract immature embryos and the best culture medium to obtain their complete development were analyzed. Embryos of Pardina, B1181 (microsperma type), B1051 and A1145 (macrosperma type) were collected at 15, 18, 21, and 24 days after pollination (DAP) and cultured on MS medium with five different concentrations of 6-benzylaminopurine (BAP) (0–0.025–0.05–0.1–0.25 mg L−1). An ANOVA test among genotypes, media, DAP and their interactions was performed. Genotypes, DAP and its interaction showed significant effects on the percentage of shoot production (F = 61.8; F = 79.3; F = 8.5; p < 0.01) and germination (F = 70.7; F = 69.8; F = 3.9; p < 0.01). Medium effect was only significant for germination (F = 8.7; p < 0.01). The microsperma genotypes gave higher percentages of shoot production (>80 %) and germination (>70 %). Although in vitro culture efficiency increased with DAP, 18 DAP was selected due to its high percentages of germination (13–70 %). The medium without BAP was the most suitable for embryo complete development (41–87 %). All plants obtained were morphologically normal and fertile. Using this approach, four generations per year were obtained allowing a rapid development of RILs.
Fil: Bermejo, Carolina Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Gatti, Ileana. Consejo de Investigadores de la Universidad Nacional de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina
Fil: Cointry Peix, Enrique Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina
description Breeding in lentil involves hybridization followed by different selection methods and requires 10 years to obtain a cultivar, as only one field generation per year can be produced. To shorten the breeding time it is essential to use biotechnological methods such as in vitro embryo culture combined with SSD method since only one seed is enough to produce the next generation. An efficient in vitro–in vivo system was developed. The best time to extract immature embryos and the best culture medium to obtain their complete development were analyzed. Embryos of Pardina, B1181 (microsperma type), B1051 and A1145 (macrosperma type) were collected at 15, 18, 21, and 24 days after pollination (DAP) and cultured on MS medium with five different concentrations of 6-benzylaminopurine (BAP) (0–0.025–0.05–0.1–0.25 mg L−1). An ANOVA test among genotypes, media, DAP and their interactions was performed. Genotypes, DAP and its interaction showed significant effects on the percentage of shoot production (F = 61.8; F = 79.3; F = 8.5; p < 0.01) and germination (F = 70.7; F = 69.8; F = 3.9; p < 0.01). Medium effect was only significant for germination (F = 8.7; p < 0.01). The microsperma genotypes gave higher percentages of shoot production (>80 %) and germination (>70 %). Although in vitro culture efficiency increased with DAP, 18 DAP was selected due to its high percentages of germination (13–70 %). The medium without BAP was the most suitable for embryo complete development (41–87 %). All plants obtained were morphologically normal and fertile. Using this approach, four generations per year were obtained allowing a rapid development of RILs.
publishDate 2016
dc.date.none.fl_str_mv 2016-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/52851
Bermejo, Carolina Julieta; Gatti, Ileana; Cointry Peix, Enrique Luis; In vitro embryo culture to shorten the breeding cycle in lentil (Lens culinaris Medik); Springer; Plant Cell, Tissue and Organ Culture; 127; 3; 12-2016; 585-590
0167-6857
CONICET Digital
CONICET
url http://hdl.handle.net/11336/52851
identifier_str_mv Bermejo, Carolina Julieta; Gatti, Ileana; Cointry Peix, Enrique Luis; In vitro embryo culture to shorten the breeding cycle in lentil (Lens culinaris Medik); Springer; Plant Cell, Tissue and Organ Culture; 127; 3; 12-2016; 585-590
0167-6857
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s11240-016-1065-7
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11240-016-1065-7
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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