Video imaging of the sperm acrosome reaction during in vitro fertilization
- Autores
- Hirohashi, Noritaka; Gerton, George L.; Buffone, Mariano Gabriel
- Año de publicación
- 2011
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Mammalian spermatozoa become competent for fusion with oocytes while traveling through the female reproductive tract and the oocyte´s extracellular investments. Recent studies highlighted the molecular mechanism of the sperm´s interactions with the zona pellucida (ZP), the extracellular coat surrounding the oocyte. Fertilizing spermatozoa initiate the sperm acrosome reaction (AR), essential for zona penetration and fusion with the oocyte plasma membrane, before they reach the ZP. However, the exact condition of spermatozoa that leads to successful penetration of the ZP remains unknown. We performed microscopic observations of in vitro fertilization with genetically (EGFP) and chemically (antibody and lectin) labeled spermatozoa to monitor the progression of the AR. Spermatozoa exhibiting EGFP(-)/PNA(+) prior to binding to the ZP initiated zona penetration. This result suggests that spermatozoa that have undergone the AR are still capable of binding and penetrating the ZP.
Fil: Hirohashi, Noritaka. Ochanomizu University; Japón
Fil: Gerton, George L.. University of Pennsylvania; Estados Unidos
Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina - Materia
-
ACROSOMAL EXOCYTOSIS
SPERM
FERTILIZATION
ACROSOME - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/10487
Ver los metadatos del registro completo
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Video imaging of the sperm acrosome reaction during in vitro fertilizationHirohashi, NoritakaGerton, George L.Buffone, Mariano GabrielACROSOMAL EXOCYTOSISSPERMFERTILIZATIONACROSOMEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Mammalian spermatozoa become competent for fusion with oocytes while traveling through the female reproductive tract and the oocyte´s extracellular investments. Recent studies highlighted the molecular mechanism of the sperm´s interactions with the zona pellucida (ZP), the extracellular coat surrounding the oocyte. Fertilizing spermatozoa initiate the sperm acrosome reaction (AR), essential for zona penetration and fusion with the oocyte plasma membrane, before they reach the ZP. However, the exact condition of spermatozoa that leads to successful penetration of the ZP remains unknown. We performed microscopic observations of in vitro fertilization with genetically (EGFP) and chemically (antibody and lectin) labeled spermatozoa to monitor the progression of the AR. Spermatozoa exhibiting EGFP(-)/PNA(+) prior to binding to the ZP initiated zona penetration. This result suggests that spermatozoa that have undergone the AR are still capable of binding and penetrating the ZP.Fil: Hirohashi, Noritaka. Ochanomizu University; JapónFil: Gerton, George L.. University of Pennsylvania; Estados UnidosFil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaLandes Bioscience2011-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/10487Hirohashi, Noritaka; Gerton, George L.; Buffone, Mariano Gabriel; Video imaging of the sperm acrosome reaction during in vitro fertilization; Landes Bioscience; Communicative and Integrative Biology; 4; 4; 7-2011; 471-4761942-08891942-0889enginfo:eu-repo/semantics/altIdentifier/doi/10.4161/cib.4.4.15636info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3181525/info:eu-repo/semantics/openAccessAtribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)https://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:48:20Zoai:ri.conicet.gov.ar:11336/10487instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:48:20.755CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Video imaging of the sperm acrosome reaction during in vitro fertilization |
title |
Video imaging of the sperm acrosome reaction during in vitro fertilization |
spellingShingle |
Video imaging of the sperm acrosome reaction during in vitro fertilization Hirohashi, Noritaka ACROSOMAL EXOCYTOSIS SPERM FERTILIZATION ACROSOME |
title_short |
Video imaging of the sperm acrosome reaction during in vitro fertilization |
title_full |
Video imaging of the sperm acrosome reaction during in vitro fertilization |
title_fullStr |
Video imaging of the sperm acrosome reaction during in vitro fertilization |
title_full_unstemmed |
Video imaging of the sperm acrosome reaction during in vitro fertilization |
title_sort |
Video imaging of the sperm acrosome reaction during in vitro fertilization |
dc.creator.none.fl_str_mv |
Hirohashi, Noritaka Gerton, George L. Buffone, Mariano Gabriel |
author |
Hirohashi, Noritaka |
author_facet |
Hirohashi, Noritaka Gerton, George L. Buffone, Mariano Gabriel |
author_role |
author |
author2 |
Gerton, George L. Buffone, Mariano Gabriel |
author2_role |
author author |
dc.subject.none.fl_str_mv |
ACROSOMAL EXOCYTOSIS SPERM FERTILIZATION ACROSOME |
topic |
ACROSOMAL EXOCYTOSIS SPERM FERTILIZATION ACROSOME |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Mammalian spermatozoa become competent for fusion with oocytes while traveling through the female reproductive tract and the oocyte´s extracellular investments. Recent studies highlighted the molecular mechanism of the sperm´s interactions with the zona pellucida (ZP), the extracellular coat surrounding the oocyte. Fertilizing spermatozoa initiate the sperm acrosome reaction (AR), essential for zona penetration and fusion with the oocyte plasma membrane, before they reach the ZP. However, the exact condition of spermatozoa that leads to successful penetration of the ZP remains unknown. We performed microscopic observations of in vitro fertilization with genetically (EGFP) and chemically (antibody and lectin) labeled spermatozoa to monitor the progression of the AR. Spermatozoa exhibiting EGFP(-)/PNA(+) prior to binding to the ZP initiated zona penetration. This result suggests that spermatozoa that have undergone the AR are still capable of binding and penetrating the ZP. Fil: Hirohashi, Noritaka. Ochanomizu University; Japón Fil: Gerton, George L.. University of Pennsylvania; Estados Unidos Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina |
description |
Mammalian spermatozoa become competent for fusion with oocytes while traveling through the female reproductive tract and the oocyte´s extracellular investments. Recent studies highlighted the molecular mechanism of the sperm´s interactions with the zona pellucida (ZP), the extracellular coat surrounding the oocyte. Fertilizing spermatozoa initiate the sperm acrosome reaction (AR), essential for zona penetration and fusion with the oocyte plasma membrane, before they reach the ZP. However, the exact condition of spermatozoa that leads to successful penetration of the ZP remains unknown. We performed microscopic observations of in vitro fertilization with genetically (EGFP) and chemically (antibody and lectin) labeled spermatozoa to monitor the progression of the AR. Spermatozoa exhibiting EGFP(-)/PNA(+) prior to binding to the ZP initiated zona penetration. This result suggests that spermatozoa that have undergone the AR are still capable of binding and penetrating the ZP. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-07 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/10487 Hirohashi, Noritaka; Gerton, George L.; Buffone, Mariano Gabriel; Video imaging of the sperm acrosome reaction during in vitro fertilization; Landes Bioscience; Communicative and Integrative Biology; 4; 4; 7-2011; 471-476 1942-0889 1942-0889 |
url |
http://hdl.handle.net/11336/10487 |
identifier_str_mv |
Hirohashi, Noritaka; Gerton, George L.; Buffone, Mariano Gabriel; Video imaging of the sperm acrosome reaction during in vitro fertilization; Landes Bioscience; Communicative and Integrative Biology; 4; 4; 7-2011; 471-476 1942-0889 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.4161/cib.4.4.15636 info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3181525/ |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR) https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR) https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Landes Bioscience |
publisher.none.fl_str_mv |
Landes Bioscience |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842268919273881600 |
score |
13.13397 |