Video imaging of the sperm acrosome reaction during in vitro fertilization

Autores
Hirohashi, Noritaka; Gerton, George L.; Buffone, Mariano Gabriel
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Mammalian spermatozoa become competent for fusion with oocytes while traveling through the female reproductive tract and the oocyte´s extracellular investments. Recent studies highlighted the molecular mechanism of the sperm´s interactions with the zona pellucida (ZP), the extracellular coat surrounding the oocyte. Fertilizing spermatozoa initiate the sperm acrosome reaction (AR), essential for zona penetration and fusion with the oocyte plasma membrane, before they reach the ZP. However, the exact condition of spermatozoa that leads to successful penetration of the ZP remains unknown. We performed microscopic observations of in vitro fertilization with genetically (EGFP) and chemically (antibody and lectin) labeled spermatozoa to monitor the progression of the AR. Spermatozoa exhibiting EGFP(-)/PNA(+) prior to binding to the ZP initiated zona penetration. This result suggests that spermatozoa that have undergone the AR are still capable of binding and penetrating the ZP.
Fil: Hirohashi, Noritaka. Ochanomizu University; Japón
Fil: Gerton, George L.. University of Pennsylvania; Estados Unidos
Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Materia
ACROSOMAL EXOCYTOSIS
SPERM
FERTILIZATION
ACROSOME
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/10487
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/10487
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Video imaging of the sperm acrosome reaction during in vitro fertilizationHirohashi, NoritakaGerton, George L.Buffone, Mariano GabrielACROSOMAL EXOCYTOSISSPERMFERTILIZATIONACROSOMEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Mammalian spermatozoa become competent for fusion with oocytes while traveling through the female reproductive tract and the oocyte´s extracellular investments. Recent studies highlighted the molecular mechanism of the sperm´s interactions with the zona pellucida (ZP), the extracellular coat surrounding the oocyte. Fertilizing spermatozoa initiate the sperm acrosome reaction (AR), essential for zona penetration and fusion with the oocyte plasma membrane, before they reach the ZP. However, the exact condition of spermatozoa that leads to successful penetration of the ZP remains unknown. We performed microscopic observations of in vitro fertilization with genetically (EGFP) and chemically (antibody and lectin) labeled spermatozoa to monitor the progression of the AR. Spermatozoa exhibiting EGFP(-)/PNA(+) prior to binding to the ZP initiated zona penetration. This result suggests that spermatozoa that have undergone the AR are still capable of binding and penetrating the ZP.Fil: Hirohashi, Noritaka. Ochanomizu University; JapónFil: Gerton, George L.. University of Pennsylvania; Estados UnidosFil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaLandes Bioscience2011-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/10487Hirohashi, Noritaka; Gerton, George L.; Buffone, Mariano Gabriel; Video imaging of the sperm acrosome reaction during in vitro fertilization; Landes Bioscience; Communicative and Integrative Biology; 4; 4; 7-2011; 471-4761942-08891942-0889enginfo:eu-repo/semantics/altIdentifier/doi/10.4161/cib.4.4.15636info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3181525/info:eu-repo/semantics/openAccessAtribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)https://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:41:27Zoai:ri.conicet.gov.ar:11336/10487instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:41:27.784CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Video imaging of the sperm acrosome reaction during in vitro fertilization
title Video imaging of the sperm acrosome reaction during in vitro fertilization
spellingShingle Video imaging of the sperm acrosome reaction during in vitro fertilization
Hirohashi, Noritaka
ACROSOMAL EXOCYTOSIS
SPERM
FERTILIZATION
ACROSOME
title_short Video imaging of the sperm acrosome reaction during in vitro fertilization
title_full Video imaging of the sperm acrosome reaction during in vitro fertilization
title_fullStr Video imaging of the sperm acrosome reaction during in vitro fertilization
title_full_unstemmed Video imaging of the sperm acrosome reaction during in vitro fertilization
title_sort Video imaging of the sperm acrosome reaction during in vitro fertilization
dc.creator.none.fl_str_mv Hirohashi, Noritaka
Gerton, George L.
Buffone, Mariano Gabriel
author Hirohashi, Noritaka
author_facet Hirohashi, Noritaka
Gerton, George L.
Buffone, Mariano Gabriel
author_role author
author2 Gerton, George L.
Buffone, Mariano Gabriel
author2_role author
author
dc.subject.none.fl_str_mv ACROSOMAL EXOCYTOSIS
SPERM
FERTILIZATION
ACROSOME
topic ACROSOMAL EXOCYTOSIS
SPERM
FERTILIZATION
ACROSOME
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Mammalian spermatozoa become competent for fusion with oocytes while traveling through the female reproductive tract and the oocyte´s extracellular investments. Recent studies highlighted the molecular mechanism of the sperm´s interactions with the zona pellucida (ZP), the extracellular coat surrounding the oocyte. Fertilizing spermatozoa initiate the sperm acrosome reaction (AR), essential for zona penetration and fusion with the oocyte plasma membrane, before they reach the ZP. However, the exact condition of spermatozoa that leads to successful penetration of the ZP remains unknown. We performed microscopic observations of in vitro fertilization with genetically (EGFP) and chemically (antibody and lectin) labeled spermatozoa to monitor the progression of the AR. Spermatozoa exhibiting EGFP(-)/PNA(+) prior to binding to the ZP initiated zona penetration. This result suggests that spermatozoa that have undergone the AR are still capable of binding and penetrating the ZP.
Fil: Hirohashi, Noritaka. Ochanomizu University; Japón
Fil: Gerton, George L.. University of Pennsylvania; Estados Unidos
Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
description Mammalian spermatozoa become competent for fusion with oocytes while traveling through the female reproductive tract and the oocyte´s extracellular investments. Recent studies highlighted the molecular mechanism of the sperm´s interactions with the zona pellucida (ZP), the extracellular coat surrounding the oocyte. Fertilizing spermatozoa initiate the sperm acrosome reaction (AR), essential for zona penetration and fusion with the oocyte plasma membrane, before they reach the ZP. However, the exact condition of spermatozoa that leads to successful penetration of the ZP remains unknown. We performed microscopic observations of in vitro fertilization with genetically (EGFP) and chemically (antibody and lectin) labeled spermatozoa to monitor the progression of the AR. Spermatozoa exhibiting EGFP(-)/PNA(+) prior to binding to the ZP initiated zona penetration. This result suggests that spermatozoa that have undergone the AR are still capable of binding and penetrating the ZP.
publishDate 2011
dc.date.none.fl_str_mv 2011-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/10487
Hirohashi, Noritaka; Gerton, George L.; Buffone, Mariano Gabriel; Video imaging of the sperm acrosome reaction during in vitro fertilization; Landes Bioscience; Communicative and Integrative Biology; 4; 4; 7-2011; 471-476
1942-0889
1942-0889
url http://hdl.handle.net/11336/10487
identifier_str_mv Hirohashi, Noritaka; Gerton, George L.; Buffone, Mariano Gabriel; Video imaging of the sperm acrosome reaction during in vitro fertilization; Landes Bioscience; Communicative and Integrative Biology; 4; 4; 7-2011; 471-476
1942-0889
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4161/cib.4.4.15636
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3181525/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Landes Bioscience
publisher.none.fl_str_mv Landes Bioscience
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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