Development of an intestinal epithelial cell line and organoids derived from the same swine and characterization of their antiviral responses

Autores
Matsumoto, Kaho; Namai, Fu; Miyazaki, Ayako; Imamura, Yoshiya; Fukuyama, Kohtaro; Ikeda Ohtsubo, Wakako; Nishiyama, Keita; Villena, Julio Cesar; Miyazama, Kohtaro; Kitazawa, Haruki
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Intestinal homeostasis and integrity are important factors for maintaining host health. This study established intestinal epithelial cell lines and organoids from the same swine jejunal crypts to develop seamless swine intestinal in vitro evaluation systems. The study evaluated the proliferative capacity and tight junction formation of the epithelial cell line and characterized the cell differentiation potential of the intestinal organoids. The evaluation systems were subsequently exposed to the TLR3 agonist poly(I:C) to simulate viral infections and assess the antiviral responses. The results demonstrated no differences in the response to type I interferons. There were, however, significant differences in the expression of interferon-stimulated genes. This study collectively introduced a flexible evaluation system using cell lines and organoids and revealed notable differences in the expression of interferon-stimulated genes, highlighting the complexity of the immune responses in these in vitro systems and the importance of intestinal heterogeneity in assessing viral responses.
Fil: Matsumoto, Kaho. Tohoku University; Japón
Fil: Namai, Fu. Tohoku University; Japón
Fil: Miyazaki, Ayako. National Agriculture and Food Research Organization. National Institute of Animal Health. Division of Infectious Animal Disease Research; Japón
Fil: Imamura, Yoshiya. Tohoku University; Japón
Fil: Fukuyama, Kohtaro. Tohoku University; Japón
Fil: Ikeda Ohtsubo, Wakako. Tohoku University; Japón
Fil: Nishiyama, Keita. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón
Fil: Miyazama, Kohtaro. National Agriculture And Food Organization. Central Region Agricultural Research Center.; Japón
Fil: Kitazawa, Haruki. Tohoku University; Japón
Materia
SWINE SMALL INTESTINE
SWINE INTESTINAL EPITHELIAL CELL
SWINE JEJUNAL ORGANOID
ANTIVIRAL RESPONSE
POLY(I:C) RESPONSE
TLR-3 AGONIST
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/262984
Acceder
id CONICETDig_02ab40b4498ef1064a77100440c90c54
oai_identifier_str oai:ri.conicet.gov.ar:11336/262984
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Development of an intestinal epithelial cell line and organoids derived from the same swine and characterization of their antiviral responsesMatsumoto, KahoNamai, FuMiyazaki, AyakoImamura, YoshiyaFukuyama, KohtaroIkeda Ohtsubo, WakakoNishiyama, KeitaVillena, Julio CesarMiyazama, KohtaroKitazawa, HarukiSWINE SMALL INTESTINESWINE INTESTINAL EPITHELIAL CELLSWINE JEJUNAL ORGANOIDANTIVIRAL RESPONSEPOLY(I:C) RESPONSETLR-3 AGONISThttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Intestinal homeostasis and integrity are important factors for maintaining host health. This study established intestinal epithelial cell lines and organoids from the same swine jejunal crypts to develop seamless swine intestinal in vitro evaluation systems. The study evaluated the proliferative capacity and tight junction formation of the epithelial cell line and characterized the cell differentiation potential of the intestinal organoids. The evaluation systems were subsequently exposed to the TLR3 agonist poly(I:C) to simulate viral infections and assess the antiviral responses. The results demonstrated no differences in the response to type I interferons. There were, however, significant differences in the expression of interferon-stimulated genes. This study collectively introduced a flexible evaluation system using cell lines and organoids and revealed notable differences in the expression of interferon-stimulated genes, highlighting the complexity of the immune responses in these in vitro systems and the importance of intestinal heterogeneity in assessing viral responses.Fil: Matsumoto, Kaho. Tohoku University; JapónFil: Namai, Fu. Tohoku University; JapónFil: Miyazaki, Ayako. National Agriculture and Food Research Organization. National Institute of Animal Health. Division of Infectious Animal Disease Research; JapónFil: Imamura, Yoshiya. Tohoku University; JapónFil: Fukuyama, Kohtaro. Tohoku University; JapónFil: Ikeda Ohtsubo, Wakako. Tohoku University; JapónFil: Nishiyama, Keita. Tohoku University; JapónFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; JapónFil: Miyazama, Kohtaro. National Agriculture And Food Organization. Central Region Agricultural Research Center.; JapónFil: Kitazawa, Haruki. Tohoku University; JapónBMFH Press2024-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/262984Matsumoto, Kaho; Namai, Fu; Miyazaki, Ayako; Imamura, Yoshiya; Fukuyama, Kohtaro; et al.; Development of an intestinal epithelial cell line and organoids derived from the same swine and characterization of their antiviral responses; BMFH Press; Bioscience of microbiota, food and health; 43; 4; 5-2024; 342-3512186-69532186-3342CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.jstage.jst.go.jp/article/bmfh/advpub/0/advpub_2024-0046/_articleinfo:eu-repo/semantics/altIdentifier/doi/10.12938/bmfh.2024-0046info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:37:20Zoai:ri.conicet.gov.ar:11336/262984instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:37:20.731CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development of an intestinal epithelial cell line and organoids derived from the same swine and characterization of their antiviral responses
title Development of an intestinal epithelial cell line and organoids derived from the same swine and characterization of their antiviral responses
spellingShingle Development of an intestinal epithelial cell line and organoids derived from the same swine and characterization of their antiviral responses
Matsumoto, Kaho
SWINE SMALL INTESTINE
SWINE INTESTINAL EPITHELIAL CELL
SWINE JEJUNAL ORGANOID
ANTIVIRAL RESPONSE
POLY(I:C) RESPONSE
TLR-3 AGONIST
title_short Development of an intestinal epithelial cell line and organoids derived from the same swine and characterization of their antiviral responses
title_full Development of an intestinal epithelial cell line and organoids derived from the same swine and characterization of their antiviral responses
title_fullStr Development of an intestinal epithelial cell line and organoids derived from the same swine and characterization of their antiviral responses
title_full_unstemmed Development of an intestinal epithelial cell line and organoids derived from the same swine and characterization of their antiviral responses
title_sort Development of an intestinal epithelial cell line and organoids derived from the same swine and characterization of their antiviral responses
dc.creator.none.fl_str_mv Matsumoto, Kaho
Namai, Fu
Miyazaki, Ayako
Imamura, Yoshiya
Fukuyama, Kohtaro
Ikeda Ohtsubo, Wakako
Nishiyama, Keita
Villena, Julio Cesar
Miyazama, Kohtaro
Kitazawa, Haruki
author Matsumoto, Kaho
author_facet Matsumoto, Kaho
Namai, Fu
Miyazaki, Ayako
Imamura, Yoshiya
Fukuyama, Kohtaro
Ikeda Ohtsubo, Wakako
Nishiyama, Keita
Villena, Julio Cesar
Miyazama, Kohtaro
Kitazawa, Haruki
author_role author
author2 Namai, Fu
Miyazaki, Ayako
Imamura, Yoshiya
Fukuyama, Kohtaro
Ikeda Ohtsubo, Wakako
Nishiyama, Keita
Villena, Julio Cesar
Miyazama, Kohtaro
Kitazawa, Haruki
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv SWINE SMALL INTESTINE
SWINE INTESTINAL EPITHELIAL CELL
SWINE JEJUNAL ORGANOID
ANTIVIRAL RESPONSE
POLY(I:C) RESPONSE
TLR-3 AGONIST
topic SWINE SMALL INTESTINE
SWINE INTESTINAL EPITHELIAL CELL
SWINE JEJUNAL ORGANOID
ANTIVIRAL RESPONSE
POLY(I:C) RESPONSE
TLR-3 AGONIST
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Intestinal homeostasis and integrity are important factors for maintaining host health. This study established intestinal epithelial cell lines and organoids from the same swine jejunal crypts to develop seamless swine intestinal in vitro evaluation systems. The study evaluated the proliferative capacity and tight junction formation of the epithelial cell line and characterized the cell differentiation potential of the intestinal organoids. The evaluation systems were subsequently exposed to the TLR3 agonist poly(I:C) to simulate viral infections and assess the antiviral responses. The results demonstrated no differences in the response to type I interferons. There were, however, significant differences in the expression of interferon-stimulated genes. This study collectively introduced a flexible evaluation system using cell lines and organoids and revealed notable differences in the expression of interferon-stimulated genes, highlighting the complexity of the immune responses in these in vitro systems and the importance of intestinal heterogeneity in assessing viral responses.
Fil: Matsumoto, Kaho. Tohoku University; Japón
Fil: Namai, Fu. Tohoku University; Japón
Fil: Miyazaki, Ayako. National Agriculture and Food Research Organization. National Institute of Animal Health. Division of Infectious Animal Disease Research; Japón
Fil: Imamura, Yoshiya. Tohoku University; Japón
Fil: Fukuyama, Kohtaro. Tohoku University; Japón
Fil: Ikeda Ohtsubo, Wakako. Tohoku University; Japón
Fil: Nishiyama, Keita. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón
Fil: Miyazama, Kohtaro. National Agriculture And Food Organization. Central Region Agricultural Research Center.; Japón
Fil: Kitazawa, Haruki. Tohoku University; Japón
description Intestinal homeostasis and integrity are important factors for maintaining host health. This study established intestinal epithelial cell lines and organoids from the same swine jejunal crypts to develop seamless swine intestinal in vitro evaluation systems. The study evaluated the proliferative capacity and tight junction formation of the epithelial cell line and characterized the cell differentiation potential of the intestinal organoids. The evaluation systems were subsequently exposed to the TLR3 agonist poly(I:C) to simulate viral infections and assess the antiviral responses. The results demonstrated no differences in the response to type I interferons. There were, however, significant differences in the expression of interferon-stimulated genes. This study collectively introduced a flexible evaluation system using cell lines and organoids and revealed notable differences in the expression of interferon-stimulated genes, highlighting the complexity of the immune responses in these in vitro systems and the importance of intestinal heterogeneity in assessing viral responses.
publishDate 2024
dc.date.none.fl_str_mv 2024-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/262984
Matsumoto, Kaho; Namai, Fu; Miyazaki, Ayako; Imamura, Yoshiya; Fukuyama, Kohtaro; et al.; Development of an intestinal epithelial cell line and organoids derived from the same swine and characterization of their antiviral responses; BMFH Press; Bioscience of microbiota, food and health; 43; 4; 5-2024; 342-351
2186-6953
2186-3342
CONICET Digital
CONICET
url http://hdl.handle.net/11336/262984
identifier_str_mv Matsumoto, Kaho; Namai, Fu; Miyazaki, Ayako; Imamura, Yoshiya; Fukuyama, Kohtaro; et al.; Development of an intestinal epithelial cell line and organoids derived from the same swine and characterization of their antiviral responses; BMFH Press; Bioscience of microbiota, food and health; 43; 4; 5-2024; 342-351
2186-6953
2186-3342
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.jstage.jst.go.jp/article/bmfh/advpub/0/advpub_2024-0046/_article
info:eu-repo/semantics/altIdentifier/doi/10.12938/bmfh.2024-0046
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv BMFH Press
publisher.none.fl_str_mv BMFH Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083494788202496
score 13.22299

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