Simultaneous detection of bee viruses by multiplex PCR

Autores
Sguazza, Guillermo Hernán; Reynaldi, Francisco José; Galosi, Cecilia Monica; Pecoraro, Marcelo Ricardo
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Honey bee mortality is a serious problem that beekeepers in Argentina have had to face during the last 3 years. Itis known thatthe consequence ofthe complex interactions between environmental and beekeeping parameters added to the effect of different disease agents such as viruses, bacteria, fungi and parasitic mites may result in a sudden collapse of the colony. In addition, multiple viral infections are detected frequently concomitantly in bee colonies. The aim of this study was to establish a multiplex polymerase chain reaction method for rapid and simultaneous detection of the most prevalent bee viruses. This multiplex PCR assay will provide specific, rapid and reliable results and allow for the cost effective detection of a particular virus as well as multiple virus infections in a single reaction tube. This method could be a helpful tool in the surveillance of the most frequently found bee viruses and to study the dynamics and the interactions of the virus populations within colonies.
Fil: Sguazza, Guillermo Hernán. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Reynaldi, Francisco José. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Galosi, Cecilia Monica. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Pecoraro, Marcelo Ricardo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Apis Mellifera
Bee Viruses
Multiplex Rt-Pcr
Detection
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/23204
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Simultaneous detection of bee viruses by multiplex PCRSguazza, Guillermo HernánReynaldi, Francisco JoséGalosi, Cecilia MonicaPecoraro, Marcelo RicardoApis MelliferaBee VirusesMultiplex Rt-PcrDetectionhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Honey bee mortality is a serious problem that beekeepers in Argentina have had to face during the last 3 years. Itis known thatthe consequence ofthe complex interactions between environmental and beekeeping parameters added to the effect of different disease agents such as viruses, bacteria, fungi and parasitic mites may result in a sudden collapse of the colony. In addition, multiple viral infections are detected frequently concomitantly in bee colonies. The aim of this study was to establish a multiplex polymerase chain reaction method for rapid and simultaneous detection of the most prevalent bee viruses. This multiplex PCR assay will provide specific, rapid and reliable results and allow for the cost effective detection of a particular virus as well as multiple virus infections in a single reaction tube. This method could be a helpful tool in the surveillance of the most frequently found bee viruses and to study the dynamics and the interactions of the virus populations within colonies.Fil: Sguazza, Guillermo Hernán. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Reynaldi, Francisco José. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Galosi, Cecilia Monica. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Pecoraro, Marcelo Ricardo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Science2013-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/23204Sguazza, Guillermo Hernán; Reynaldi, Francisco José; Galosi, Cecilia Monica; Pecoraro, Marcelo Ricardo; Simultaneous detection of bee viruses by multiplex PCR ; Elsevier Science; Journal of Virological Methods; 194; 1-2; 8-2013; 102-1060166-0934CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0166093413003352info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jviromet.2013.08.003info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:10:39Zoai:ri.conicet.gov.ar:11336/23204instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:10:39.49CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Simultaneous detection of bee viruses by multiplex PCR
title Simultaneous detection of bee viruses by multiplex PCR
spellingShingle Simultaneous detection of bee viruses by multiplex PCR
Sguazza, Guillermo Hernán
Apis Mellifera
Bee Viruses
Multiplex Rt-Pcr
Detection
title_short Simultaneous detection of bee viruses by multiplex PCR
title_full Simultaneous detection of bee viruses by multiplex PCR
title_fullStr Simultaneous detection of bee viruses by multiplex PCR
title_full_unstemmed Simultaneous detection of bee viruses by multiplex PCR
title_sort Simultaneous detection of bee viruses by multiplex PCR
dc.creator.none.fl_str_mv Sguazza, Guillermo Hernán
Reynaldi, Francisco José
Galosi, Cecilia Monica
Pecoraro, Marcelo Ricardo
author Sguazza, Guillermo Hernán
author_facet Sguazza, Guillermo Hernán
Reynaldi, Francisco José
Galosi, Cecilia Monica
Pecoraro, Marcelo Ricardo
author_role author
author2 Reynaldi, Francisco José
Galosi, Cecilia Monica
Pecoraro, Marcelo Ricardo
author2_role author
author
author
dc.subject.none.fl_str_mv Apis Mellifera
Bee Viruses
Multiplex Rt-Pcr
Detection
topic Apis Mellifera
Bee Viruses
Multiplex Rt-Pcr
Detection
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Honey bee mortality is a serious problem that beekeepers in Argentina have had to face during the last 3 years. Itis known thatthe consequence ofthe complex interactions between environmental and beekeeping parameters added to the effect of different disease agents such as viruses, bacteria, fungi and parasitic mites may result in a sudden collapse of the colony. In addition, multiple viral infections are detected frequently concomitantly in bee colonies. The aim of this study was to establish a multiplex polymerase chain reaction method for rapid and simultaneous detection of the most prevalent bee viruses. This multiplex PCR assay will provide specific, rapid and reliable results and allow for the cost effective detection of a particular virus as well as multiple virus infections in a single reaction tube. This method could be a helpful tool in the surveillance of the most frequently found bee viruses and to study the dynamics and the interactions of the virus populations within colonies.
Fil: Sguazza, Guillermo Hernán. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Reynaldi, Francisco José. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Galosi, Cecilia Monica. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Pecoraro, Marcelo Ricardo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Honey bee mortality is a serious problem that beekeepers in Argentina have had to face during the last 3 years. Itis known thatthe consequence ofthe complex interactions between environmental and beekeeping parameters added to the effect of different disease agents such as viruses, bacteria, fungi and parasitic mites may result in a sudden collapse of the colony. In addition, multiple viral infections are detected frequently concomitantly in bee colonies. The aim of this study was to establish a multiplex polymerase chain reaction method for rapid and simultaneous detection of the most prevalent bee viruses. This multiplex PCR assay will provide specific, rapid and reliable results and allow for the cost effective detection of a particular virus as well as multiple virus infections in a single reaction tube. This method could be a helpful tool in the surveillance of the most frequently found bee viruses and to study the dynamics and the interactions of the virus populations within colonies.
publishDate 2013
dc.date.none.fl_str_mv 2013-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/23204
Sguazza, Guillermo Hernán; Reynaldi, Francisco José; Galosi, Cecilia Monica; Pecoraro, Marcelo Ricardo; Simultaneous detection of bee viruses by multiplex PCR ; Elsevier Science; Journal of Virological Methods; 194; 1-2; 8-2013; 102-106
0166-0934
CONICET Digital
CONICET
url http://hdl.handle.net/11336/23204
identifier_str_mv Sguazza, Guillermo Hernán; Reynaldi, Francisco José; Galosi, Cecilia Monica; Pecoraro, Marcelo Ricardo; Simultaneous detection of bee viruses by multiplex PCR ; Elsevier Science; Journal of Virological Methods; 194; 1-2; 8-2013; 102-106
0166-0934
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0166093413003352
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jviromet.2013.08.003
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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