Molecular detection of honeybee viruses in Ecuador

Autores
Bravi, Maria Emilia; Avalos, Jorge; Rosero, Hugo; Maldonado, Gerald; Reynaldi, Francisco José; Genchi García, María Laura
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aim of study: The honeybee, Apis mellifera, is one of the most important pollinators in the world. Apicultural activity and pollination services have been affected by the decline in the honeybee population, which may be due to the interaction of multiple risk factors, such as changes in agricultural production, use of pesticides and presence of pathogens. Viruses, in particular, are sus-pected to be drivers of colony mortality. In this scenario, the aim of this study was to determine the presence of honeybee viruses (IAPV, DWV, SBV, ABPV, BQCV, CBPV) in A. mellifera populations using a RT-mPCR assay. Area of study: Apiaries were situated in Pichincha, Ecuador. Material and methods: Samples were collected from seventeen apiaries that exhibited mortality but without specific clinical signs. Each sample comprised 15 individuals. After RNA extraction, a multiplex PCR analysis was performed for presence of six viruses (IAPV, DWV, SBV, ABPV, BQCV, CBPV). Main results: Four of the viruses (ABPV, DWV, BQCV and SBV) were found in co-infections in these colonies, with ABPV and SBV also being found in simple infections. Research highlights: To our knowledge, this is the first molecular detection of BQCV and SBV in Ecuador. These findings sug-gest that some of the above viruses could be involved in weakening these colonies.
Fil: Bravi, Maria Emilia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Avalos, Jorge. Programa Nacional Sanitario Apícola; Ecuador
Fil: Rosero, Hugo. Programa Nacional Sanitario Apícola; Ecuador
Fil: Maldonado, Gerald. Programa Nacional Sanitario Apícola; Ecuador
Fil: Reynaldi, Francisco José. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Genchi García, María Laura. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Materia
ABPV
APIS MELLIFERA
BQCV
DWV
MULTIPLEX-PCR
SBV
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/131412
Acceder
id CONICETDig_5c45f4777657ae243617aaed8b58041d
oai_identifier_str oai:ri.conicet.gov.ar:11336/131412
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Molecular detection of honeybee viruses in EcuadorBravi, Maria EmiliaAvalos, JorgeRosero, HugoMaldonado, GeraldReynaldi, Francisco JoséGenchi García, María LauraABPVAPIS MELLIFERABQCVDWVMULTIPLEX-PCRSBVhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Aim of study: The honeybee, Apis mellifera, is one of the most important pollinators in the world. Apicultural activity and pollination services have been affected by the decline in the honeybee population, which may be due to the interaction of multiple risk factors, such as changes in agricultural production, use of pesticides and presence of pathogens. Viruses, in particular, are sus-pected to be drivers of colony mortality. In this scenario, the aim of this study was to determine the presence of honeybee viruses (IAPV, DWV, SBV, ABPV, BQCV, CBPV) in A. mellifera populations using a RT-mPCR assay. Area of study: Apiaries were situated in Pichincha, Ecuador. Material and methods: Samples were collected from seventeen apiaries that exhibited mortality but without specific clinical signs. Each sample comprised 15 individuals. After RNA extraction, a multiplex PCR analysis was performed for presence of six viruses (IAPV, DWV, SBV, ABPV, BQCV, CBPV). Main results: Four of the viruses (ABPV, DWV, BQCV and SBV) were found in co-infections in these colonies, with ABPV and SBV also being found in simple infections. Research highlights: To our knowledge, this is the first molecular detection of BQCV and SBV in Ecuador. These findings sug-gest that some of the above viruses could be involved in weakening these colonies.Fil: Bravi, Maria Emilia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Avalos, Jorge. Programa Nacional Sanitario Apícola; EcuadorFil: Rosero, Hugo. Programa Nacional Sanitario Apícola; EcuadorFil: Maldonado, Gerald. Programa Nacional Sanitario Apícola; EcuadorFil: Reynaldi, Francisco José. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Genchi García, María Laura. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaSpanish National Institute for Agriculture and Food Research and Technology2020-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/131412Bravi, Maria Emilia; Avalos, Jorge; Rosero, Hugo; Maldonado, Gerald; Reynaldi, Francisco José; et al.; Molecular detection of honeybee viruses in Ecuador; Spanish National Institute for Agriculture and Food Research and Technology; Spanish Journal Of Agricultural Research; 18; 1; 1-2020; 1-51695-971XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://revistas.inia.es/index.php/sjar/article/view/15779info:eu-repo/semantics/altIdentifier/doi/10.5424/sjar/2020181-15779info:eu-repo/semantics/altIdentifier/url/https://dialnet.unirioja.es/servlet/articulo?codigo=7398678info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:24:42Zoai:ri.conicet.gov.ar:11336/131412instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:24:42.603CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular detection of honeybee viruses in Ecuador
title Molecular detection of honeybee viruses in Ecuador
spellingShingle Molecular detection of honeybee viruses in Ecuador
Bravi, Maria Emilia
ABPV
APIS MELLIFERA
BQCV
DWV
MULTIPLEX-PCR
SBV
title_short Molecular detection of honeybee viruses in Ecuador
title_full Molecular detection of honeybee viruses in Ecuador
title_fullStr Molecular detection of honeybee viruses in Ecuador
title_full_unstemmed Molecular detection of honeybee viruses in Ecuador
title_sort Molecular detection of honeybee viruses in Ecuador
dc.creator.none.fl_str_mv Bravi, Maria Emilia
Avalos, Jorge
Rosero, Hugo
Maldonado, Gerald
Reynaldi, Francisco José
Genchi García, María Laura
author Bravi, Maria Emilia
author_facet Bravi, Maria Emilia
Avalos, Jorge
Rosero, Hugo
Maldonado, Gerald
Reynaldi, Francisco José
Genchi García, María Laura
author_role author
author2 Avalos, Jorge
Rosero, Hugo
Maldonado, Gerald
Reynaldi, Francisco José
Genchi García, María Laura
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ABPV
APIS MELLIFERA
BQCV
DWV
MULTIPLEX-PCR
SBV
topic ABPV
APIS MELLIFERA
BQCV
DWV
MULTIPLEX-PCR
SBV
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Aim of study: The honeybee, Apis mellifera, is one of the most important pollinators in the world. Apicultural activity and pollination services have been affected by the decline in the honeybee population, which may be due to the interaction of multiple risk factors, such as changes in agricultural production, use of pesticides and presence of pathogens. Viruses, in particular, are sus-pected to be drivers of colony mortality. In this scenario, the aim of this study was to determine the presence of honeybee viruses (IAPV, DWV, SBV, ABPV, BQCV, CBPV) in A. mellifera populations using a RT-mPCR assay. Area of study: Apiaries were situated in Pichincha, Ecuador. Material and methods: Samples were collected from seventeen apiaries that exhibited mortality but without specific clinical signs. Each sample comprised 15 individuals. After RNA extraction, a multiplex PCR analysis was performed for presence of six viruses (IAPV, DWV, SBV, ABPV, BQCV, CBPV). Main results: Four of the viruses (ABPV, DWV, BQCV and SBV) were found in co-infections in these colonies, with ABPV and SBV also being found in simple infections. Research highlights: To our knowledge, this is the first molecular detection of BQCV and SBV in Ecuador. These findings sug-gest that some of the above viruses could be involved in weakening these colonies.
Fil: Bravi, Maria Emilia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Avalos, Jorge. Programa Nacional Sanitario Apícola; Ecuador
Fil: Rosero, Hugo. Programa Nacional Sanitario Apícola; Ecuador
Fil: Maldonado, Gerald. Programa Nacional Sanitario Apícola; Ecuador
Fil: Reynaldi, Francisco José. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Genchi García, María Laura. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
description Aim of study: The honeybee, Apis mellifera, is one of the most important pollinators in the world. Apicultural activity and pollination services have been affected by the decline in the honeybee population, which may be due to the interaction of multiple risk factors, such as changes in agricultural production, use of pesticides and presence of pathogens. Viruses, in particular, are sus-pected to be drivers of colony mortality. In this scenario, the aim of this study was to determine the presence of honeybee viruses (IAPV, DWV, SBV, ABPV, BQCV, CBPV) in A. mellifera populations using a RT-mPCR assay. Area of study: Apiaries were situated in Pichincha, Ecuador. Material and methods: Samples were collected from seventeen apiaries that exhibited mortality but without specific clinical signs. Each sample comprised 15 individuals. After RNA extraction, a multiplex PCR analysis was performed for presence of six viruses (IAPV, DWV, SBV, ABPV, BQCV, CBPV). Main results: Four of the viruses (ABPV, DWV, BQCV and SBV) were found in co-infections in these colonies, with ABPV and SBV also being found in simple infections. Research highlights: To our knowledge, this is the first molecular detection of BQCV and SBV in Ecuador. These findings sug-gest that some of the above viruses could be involved in weakening these colonies.
publishDate 2020
dc.date.none.fl_str_mv 2020-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/131412
Bravi, Maria Emilia; Avalos, Jorge; Rosero, Hugo; Maldonado, Gerald; Reynaldi, Francisco José; et al.; Molecular detection of honeybee viruses in Ecuador; Spanish National Institute for Agriculture and Food Research and Technology; Spanish Journal Of Agricultural Research; 18; 1; 1-2020; 1-5
1695-971X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/131412
identifier_str_mv Bravi, Maria Emilia; Avalos, Jorge; Rosero, Hugo; Maldonado, Gerald; Reynaldi, Francisco José; et al.; Molecular detection of honeybee viruses in Ecuador; Spanish National Institute for Agriculture and Food Research and Technology; Spanish Journal Of Agricultural Research; 18; 1; 1-2020; 1-5
1695-971X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://revistas.inia.es/index.php/sjar/article/view/15779
info:eu-repo/semantics/altIdentifier/doi/10.5424/sjar/2020181-15779
info:eu-repo/semantics/altIdentifier/url/https://dialnet.unirioja.es/servlet/articulo?codigo=7398678
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Spanish National Institute for Agriculture and Food Research and Technology
publisher.none.fl_str_mv Spanish National Institute for Agriculture and Food Research and Technology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082672139436032
score 12.891075

Publicaciones similares