A novel combination of γ-tocopherol-rich mixture of tocopherols and ascorbic acid restores fertility in cases of tyrosine nitration-associated male infertility in mice

Autores
Scarlata, Eleonora; Fernández, María Celia; O’Flaherty, Cristian
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Infertility is an important health problem that affects up to 16% of couples worldwide. Male infertility is responsible for 50% of the cases. Currently, a physical examination, hormone profiling and the evaluation of two consecutive semen samples (to determine the sperm concentration, motility, morphology and, in very few cases, sperm DNA integrity) are the sole tools that physicians have to evaluate infertility in men. Antioxidant therapy is often used to improve sperm quality and function in infertile men. However, there are controversial results regarding the efficacy of these treatments. Prdx6-/- male mice are subfertile, displaying significant oxidative damage in the lipids, proteins and DNA of their spermatozoa. Here, we used Prdx6-/- male mice to test whether a novel combination of tocopherols that contained 60% γ-tocopherol and ascorbic acid could restore their fertility. These mice were fed with the supplemented (Vit. Mix) or control diets. To assess sperm quality, we determined the motility, levels of lipid peroxidation, DNA oxidation and tyrosine nitration in the spermatozoa. The number of pups sired by the Prdx6-/- mice fed with the Vit. Mix diet was higher than that sired by the males fed with the control diet, and the pups’ mortality was lower. The sperm quality was improved in the males fed with the supplemented diet. We concluded that treatment with a supplement composed of tocopherols and rich in γ-tocopherol and ascorbic acid is effective in restoring fertility in cases where oxidative stress and high levels of tyrosine nitration are associated with male infertility.
Fil: Scarlata, Eleonora. McGill University; Canadá
Fil: Fernández, María Celia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; Argentina
Fil: O’Flaherty, Cristian. McGill University; Canadá
Materia
FERTILIZATION
LIPID PEROXIDATION
REACTIVE OXYGEN SPECIES
SPERMATOZOA
VITAMIN C
VITAMIN E
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/139936
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/139936
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A novel combination of γ-tocopherol-rich mixture of tocopherols and ascorbic acid restores fertility in cases of tyrosine nitration-associated male infertility in miceScarlata, EleonoraFernández, María CeliaO’Flaherty, CristianFERTILIZATIONLIPID PEROXIDATIONREACTIVE OXYGEN SPECIESSPERMATOZOAVITAMIN CVITAMIN Ehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Infertility is an important health problem that affects up to 16% of couples worldwide. Male infertility is responsible for 50% of the cases. Currently, a physical examination, hormone profiling and the evaluation of two consecutive semen samples (to determine the sperm concentration, motility, morphology and, in very few cases, sperm DNA integrity) are the sole tools that physicians have to evaluate infertility in men. Antioxidant therapy is often used to improve sperm quality and function in infertile men. However, there are controversial results regarding the efficacy of these treatments. Prdx6-/- male mice are subfertile, displaying significant oxidative damage in the lipids, proteins and DNA of their spermatozoa. Here, we used Prdx6-/- male mice to test whether a novel combination of tocopherols that contained 60% γ-tocopherol and ascorbic acid could restore their fertility. These mice were fed with the supplemented (Vit. Mix) or control diets. To assess sperm quality, we determined the motility, levels of lipid peroxidation, DNA oxidation and tyrosine nitration in the spermatozoa. The number of pups sired by the Prdx6-/- mice fed with the Vit. Mix diet was higher than that sired by the males fed with the control diet, and the pups’ mortality was lower. The sperm quality was improved in the males fed with the supplemented diet. We concluded that treatment with a supplement composed of tocopherols and rich in γ-tocopherol and ascorbic acid is effective in restoring fertility in cases where oxidative stress and high levels of tyrosine nitration are associated with male infertility.Fil: Scarlata, Eleonora. McGill University; CanadáFil: Fernández, María Celia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; ArgentinaFil: O’Flaherty, Cristian. McGill University; CanadáMolecular Diversity Preservation International2020-07-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/139936Scarlata, Eleonora; Fernández, María Celia; O’Flaherty, Cristian; A novel combination of γ-tocopherol-rich mixture of tocopherols and ascorbic acid restores fertility in cases of tyrosine nitration-associated male infertility in mice; Molecular Diversity Preservation International; Antioxidants; 9; 7; 13-7-2020; 1-122076-3921CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-3921/9/7/613info:eu-repo/semantics/altIdentifier/doi/10.3390/antiox9070613info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:47:14Zoai:ri.conicet.gov.ar:11336/139936instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:47:14.495CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A novel combination of γ-tocopherol-rich mixture of tocopherols and ascorbic acid restores fertility in cases of tyrosine nitration-associated male infertility in mice
title A novel combination of γ-tocopherol-rich mixture of tocopherols and ascorbic acid restores fertility in cases of tyrosine nitration-associated male infertility in mice
spellingShingle A novel combination of γ-tocopherol-rich mixture of tocopherols and ascorbic acid restores fertility in cases of tyrosine nitration-associated male infertility in mice
Scarlata, Eleonora
FERTILIZATION
LIPID PEROXIDATION
REACTIVE OXYGEN SPECIES
SPERMATOZOA
VITAMIN C
VITAMIN E
title_short A novel combination of γ-tocopherol-rich mixture of tocopherols and ascorbic acid restores fertility in cases of tyrosine nitration-associated male infertility in mice
title_full A novel combination of γ-tocopherol-rich mixture of tocopherols and ascorbic acid restores fertility in cases of tyrosine nitration-associated male infertility in mice
title_fullStr A novel combination of γ-tocopherol-rich mixture of tocopherols and ascorbic acid restores fertility in cases of tyrosine nitration-associated male infertility in mice
title_full_unstemmed A novel combination of γ-tocopherol-rich mixture of tocopherols and ascorbic acid restores fertility in cases of tyrosine nitration-associated male infertility in mice
title_sort A novel combination of γ-tocopherol-rich mixture of tocopherols and ascorbic acid restores fertility in cases of tyrosine nitration-associated male infertility in mice
dc.creator.none.fl_str_mv Scarlata, Eleonora
Fernández, María Celia
O’Flaherty, Cristian
author Scarlata, Eleonora
author_facet Scarlata, Eleonora
Fernández, María Celia
O’Flaherty, Cristian
author_role author
author2 Fernández, María Celia
O’Flaherty, Cristian
author2_role author
author
dc.subject.none.fl_str_mv FERTILIZATION
LIPID PEROXIDATION
REACTIVE OXYGEN SPECIES
SPERMATOZOA
VITAMIN C
VITAMIN E
topic FERTILIZATION
LIPID PEROXIDATION
REACTIVE OXYGEN SPECIES
SPERMATOZOA
VITAMIN C
VITAMIN E
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Infertility is an important health problem that affects up to 16% of couples worldwide. Male infertility is responsible for 50% of the cases. Currently, a physical examination, hormone profiling and the evaluation of two consecutive semen samples (to determine the sperm concentration, motility, morphology and, in very few cases, sperm DNA integrity) are the sole tools that physicians have to evaluate infertility in men. Antioxidant therapy is often used to improve sperm quality and function in infertile men. However, there are controversial results regarding the efficacy of these treatments. Prdx6-/- male mice are subfertile, displaying significant oxidative damage in the lipids, proteins and DNA of their spermatozoa. Here, we used Prdx6-/- male mice to test whether a novel combination of tocopherols that contained 60% γ-tocopherol and ascorbic acid could restore their fertility. These mice were fed with the supplemented (Vit. Mix) or control diets. To assess sperm quality, we determined the motility, levels of lipid peroxidation, DNA oxidation and tyrosine nitration in the spermatozoa. The number of pups sired by the Prdx6-/- mice fed with the Vit. Mix diet was higher than that sired by the males fed with the control diet, and the pups’ mortality was lower. The sperm quality was improved in the males fed with the supplemented diet. We concluded that treatment with a supplement composed of tocopherols and rich in γ-tocopherol and ascorbic acid is effective in restoring fertility in cases where oxidative stress and high levels of tyrosine nitration are associated with male infertility.
Fil: Scarlata, Eleonora. McGill University; Canadá
Fil: Fernández, María Celia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; Argentina
Fil: O’Flaherty, Cristian. McGill University; Canadá
description Infertility is an important health problem that affects up to 16% of couples worldwide. Male infertility is responsible for 50% of the cases. Currently, a physical examination, hormone profiling and the evaluation of two consecutive semen samples (to determine the sperm concentration, motility, morphology and, in very few cases, sperm DNA integrity) are the sole tools that physicians have to evaluate infertility in men. Antioxidant therapy is often used to improve sperm quality and function in infertile men. However, there are controversial results regarding the efficacy of these treatments. Prdx6-/- male mice are subfertile, displaying significant oxidative damage in the lipids, proteins and DNA of their spermatozoa. Here, we used Prdx6-/- male mice to test whether a novel combination of tocopherols that contained 60% γ-tocopherol and ascorbic acid could restore their fertility. These mice were fed with the supplemented (Vit. Mix) or control diets. To assess sperm quality, we determined the motility, levels of lipid peroxidation, DNA oxidation and tyrosine nitration in the spermatozoa. The number of pups sired by the Prdx6-/- mice fed with the Vit. Mix diet was higher than that sired by the males fed with the control diet, and the pups’ mortality was lower. The sperm quality was improved in the males fed with the supplemented diet. We concluded that treatment with a supplement composed of tocopherols and rich in γ-tocopherol and ascorbic acid is effective in restoring fertility in cases where oxidative stress and high levels of tyrosine nitration are associated with male infertility.
publishDate 2020
dc.date.none.fl_str_mv 2020-07-13
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/139936
Scarlata, Eleonora; Fernández, María Celia; O’Flaherty, Cristian; A novel combination of γ-tocopherol-rich mixture of tocopherols and ascorbic acid restores fertility in cases of tyrosine nitration-associated male infertility in mice; Molecular Diversity Preservation International; Antioxidants; 9; 7; 13-7-2020; 1-12
2076-3921
CONICET Digital
CONICET
url http://hdl.handle.net/11336/139936
identifier_str_mv Scarlata, Eleonora; Fernández, María Celia; O’Flaherty, Cristian; A novel combination of γ-tocopherol-rich mixture of tocopherols and ascorbic acid restores fertility in cases of tyrosine nitration-associated male infertility in mice; Molecular Diversity Preservation International; Antioxidants; 9; 7; 13-7-2020; 1-12
2076-3921
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-3921/9/7/613
info:eu-repo/semantics/altIdentifier/doi/10.3390/antiox9070613
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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